Abstract:
:Changes in the behavior of the sarcoplasmic reticulum (SR) in rat ventricular myocytes were investigated under conditions of metabolic inhibition using laser-scanning confocal microscopy to measure intracellular Ca(2+) and the perforated patch-clamp technique to measure SR Ca(2+) content. Metabolic inhibition had several effects on SR function, including reduced frequency of spontaneous releases of Ca(2+) (sparks and waves of Ca(2+)-induced Ca(2+) release), increased SR Ca(2+) content (79.4+/-5.7 to 115.2+/-6.6 micromol/L cell volume [mean+/-SEM; P:<0.001]), and, after a wave of Ca(2+) release, slower reuptake of Ca(2+) into the SR (rate constant of fall of Ca(2+) reduced from 8.5+/-1.1 s(-)(1) in control to 5.2+/-0.4 s(-)(1) in metabolic inhibition [P:<0.01]). Inhibition of L-type Ca(2+) channels with Cd(2+) (100 micromol/L) did not reproduce the effects of metabolic inhibition on spontaneous Ca(2+) sparks. These results are evidence of inhibition of both Ca(2+) release and reuptake mechanisms. Reduced frequency of release could be attributable to either of these effects, but the increased SR Ca(2+) content at the time of reduced frequency of spontaneous release of Ca(2+) shows that the dominant effect of metabolic inhibition is to inhibit release of Ca(2+) from the SR, allowing the accumulation of greater than normal amounts of Ca(2+). In the context of ischemia, this extra accumulation of Ca(2+) would present a risk of potentially arrhythmogenic, spontaneous release of Ca(2+) on reperfusion of the tissue.
journal_name
Circ Resjournal_title
Circulation researchauthors
Overend CL,Eisner DA,O'Neill SCdoi
10.1161/01.res.88.2.181subject
Has Abstractpub_date
2001-02-02 00:00:00pages
181-7issue
2eissn
0009-7330issn
1524-4571journal_volume
88pub_type
杂志文章abstract::Transmural heterogeneity of myocardial metabolism and function are present in the left ventricle under normal and ischemic conditions. To determine if endocardial versus epicardial differences of [Ca2+]i are also present, perfused rat heart studies using indo-1 fluorescence as an index of [Ca2+]i were performed in the...
journal_title:Circulation research
pub_type: 杂志文章
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更新日期:1990-11-01 00:00:00
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更新日期:2006-08-04 00:00:00
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更新日期:1987-10-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2013-08-30 00:00:00
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pub_type: 杂志文章
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更新日期:1994-10-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.42.2.263
更新日期:1978-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.111.249482
更新日期:2012-01-20 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.RES.0000187456.06162.cb
更新日期:2005-10-28 00:00:00
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pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.109.204040
更新日期:2009-10-23 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:2001-10-26 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:2003-03-07 00:00:00
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更新日期:1991-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2019-10-25 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:2001-04-27 00:00:00
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pub_type: 杂志文章
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更新日期:2004-06-25 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:1992-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1995-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-02-20 00:00:00
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pub_type: 杂志文章
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更新日期:2019-04-12 00:00:00
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pub_type: 杂志文章
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更新日期:2019-01-18 00:00:00