[Molecular biological methods and their consequences in taxonomy and diagnosis of dermatophytes].

Abstract:

:Trichophyton rubrum, T. mentagrophytes, T. verrucosum, Microsporum canis, M. gypseum and Epidermophyton flocoosum represent the cause of human dermatomycoses isolated most often at the University Hospital of Dermatology in Graz, Austria, between 1991 and 1998. So far, identification was mainly based on the cultivation of fungal isolates on special media as well as on the analysis of their microscopic characters. Restriction enzyme length polymorphisms (RFLPs) were now used to identify these human fungal parasites. For this purpose, internal transcribed spacers (ITS) which are located between ribosomal RNA genes have been amplified by using PCR and have afterwards been used to generate species specific RFLP patterns. By this method, a fast and reliable identification of these species was made possible. Nucleotide sequence data of this region not needed for identification have been worked out to show RFLPs in more detail.

journal_name

Mycoses

journal_title

Mycoses

authors

Blanz P,Buzina W,Ginter G,Gräser Y

subject

Has Abstract

pub_date

2000-01-01 00:00:00

pages

11-6

eissn

0933-7407

issn

1439-0507

journal_volume

43 Suppl 1

pub_type

杂志文章

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