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Measurement of proteases in human subgingival dental plaque by fluorescence polarization.

Abstract：

:Fluorescence polarization (FP) was examined as a rapid quantitative method to assay the proteases in subgingival plaque. Protease activity was measured by a decrease in FP at 0.5-min intervals over 5 min, using BODIPY-alpha-casein, a protein substrate. To quantitate activity, the least absolute deviation (LAD) slope for each assay was determined. Protease activity increased with the quantity of plaque (r=0.416, P<0.001). Of the 208 subgingival plaque samples, 87 contained detectable protease activity, with a mean of about 4 microg trypsin equivalents above a general background of 1 microg per site. The mean plaque protease activity of 89 paired samples from 15 individuals had decreased by 1.1 microg trypsin equivalents per site when measured at 8 months after tooth scaling and root planing (P<0.01). Most isolates of Porphyromonas gingivalis, Treponema denticola, Prevotella nigrescens, and Prevotella intermedia implicated in the pathogenesis of adult periodontitis exhibited high activity in the FP assay. The assay is rapid, quantitative and requires only one-tenth of the plaque sampled using a single pass with a Gracey curette at a single tooth site.

journal_name

Arch Oral Biol

journal_title

Archives of oral biology

authors

Grys EL,Schade SZ,Cohen ME,Geivelis M,Robinson PJ,Simonson LG

doi

10.1016/s0003-9969(00)00073-x

subject

Has Abstract

pub_date

2000-12-01 00:00:00

pages

1101-6

issue

12

eissn

0003-9969

issn

1879-1506

pii

S0003-9969(00)00073-X

journal_volume

45

pub_type

杂志文章

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