Expression of messenger RNA of the cardiac isoforms of troponin T and I in myopathic skeletal muscle.

Abstract:

:In the absence of clinical signs, elevated values of the cardiac isoforms of troponin T (cTnT) and I (cTnI) can be found in the serum samples of some patients with skeletal muscle myopathies; the cause is unclear. We studied the messenger RNA (mRNA) expression of cTnT and cTnI in the skeletal muscles of 24 patients with histologically proven myopathies and in 18 patients in whom a myopathy could be excluded. For cTnT- and cTnI-mRNA determination, we designed specific primer pairs for nested polymerase chain reaction. After amplification, the products were digested with 2 restriction enzymes and visualized. We found cTnT mRNA in 7 skeletal muscle biopsy specimens (6 patients with Duchenne muscular dystrophy, 1 patient with a primary sarcoglycanopathy) and cTnI mRNA in 6 (5 with Duchenne muscular dystrophy, 1 patient with a histologically negative biopsy). The mRNA of the cardiac isoforms, cTnT and cTnI, is expressed in the skeletal muscles of patients with Duchenne muscular dystrophy, but also in some other myopathies. Further studies are needed to show whether the mRNA is translated into the protein, but serum levels of cTnT and cTnI in patients with Duchenne muscular dystrophy would seem to indicate this.

journal_name

Am J Clin Pathol

authors

Messner B,Baum H,Fischer P,Quasthoff S,Neumeier D

subject

Has Abstract

pub_date

2000-10-01 00:00:00

pages

544-9

issue

4

eissn

0002-9173

issn

1943-7722

journal_volume

114

pub_type

杂志文章
  • Catenoidal shape of the interventricular septum acquired secondary to myocardial infarction.

    abstract::A catenoidal, or saddle-shaped, configuration of the interventricular septum, concave toward the left ventricular cavity in the transverse plane, but convex toward the left ventricular cavity in the apex to base plane, occurs in idiopathic hypertrophic subaortic stenosis and possibly may lead to septal hypertrophy and...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/83.1.53

    authors: Hutchins GM,Moore GW,Skoog DK

    更新日期:1985-01-01 00:00:00

  • Blue nevus of the lymph node capsule. Report of a new case with review of the literature.

    abstract::This article describes the seventh known case of blue nevus of the lymph node capsule and reviews the previously reported cases. The migration arrest hypothesis as an explanation for the occurrence of this lesion is favored rather than the "benign metastasis" hypothesis. The importance of the recognition of this pheno...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/81.3.367

    authors: Lamovec J

    更新日期:1984-03-01 00:00:00

  • Morphonuclear relationship between prostatic intraepithelial neoplasia and cancers as assessed by digital cell image analysis.

    abstract::Using digital cell image analysis performed on Feulgen-stained nuclei, the nuclear characteristics of prostatic neoplasia, ranging from benign (benign prostatic hyperplasia [BPH]), through dysplastic (prostatic intraepithelial neoplasia [PIN] 1-3), to carcinoma were studied. Four histopathologic groups were studied: g...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/96.5.628

    authors: Petein M,Michel P,van Velthoven R,Pasteels JL,Brawer MK,Davis JR,Nagle RB,Kiss R

    更新日期:1991-11-01 00:00:00

  • Protein C assay performance: an analysis of North American specialized coagulation laboratory association proficiency testing results.

    abstract::To determine the performance and frequency of protein C reagents currently used by clinical laboratories, we analyzed North American Specialized Coagulation Laboratory Association (NASCOLA) protein C proficiency testing data from 6 surveys conducted in 2009 and 2010 (2009-1 to 2009-3 and 2010-1 to 2010-3). Interlabora...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCP8MWU4QSTCLPU

    authors: Baron JM,Johnson SM,Ledford-Kraemer MR,Hayward CP,Meijer P,Van Cott EM

    更新日期:2012-06-01 00:00:00

  • Molecular and Immunohistochemical Analysis of Mucinous Cystic Neoplasm of the Liver.

    abstract:OBJECTIVES:Mucinous cystic neoplasm of the liver is characterized by neoplastic mucinous and/or biliary epithelium surrounded by ovarian-type stroma. Immunohistochemical studies have shown that the ovarian-type stroma expresses estrogen receptor, suggesting potential hormonal responsiveness. The molecular biology of mu...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/aqaa115

    authors: Van Treeck BJ,Lotfalla M,Czeczok TW,Mounajjed T,Moreira RK,Allende DS,Reid MD,Naini BV,Westerhoff M,Adsay NV,Kerr SE,Rizvi SH,Smoot RL,Liu Y,Davila J,Graham RP

    更新日期:2020-11-04 00:00:00

  • Immunophenotypic analysis of hematologic malignancy by laser scanning cytometry.

    abstract::The authors tested a newly-developed computerized laser scanning cytometer (LSC) as a means of performing immunophenotypic analysis of hematologic specimens within their community hospital. Results were compared on a case-by-case basis with parallel flow cytometric and immunohistochemical data. A total of 71 specimens...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/105.6.744

    authors: Clatch RJ,Walloch JL,Zutter MM,Kamentsky LA

    更新日期:1996-06-01 00:00:00

  • The expression of histocompatibility-related leukocyte antigens in the pathway to cervical carcinoma.

    abstract::The major histocompatibility complex probably plays a crucial role in the efficacy of the cellular immune response against virally infected cervical diseases. Therefore, the allele-specific histocompatibility-related leukocyte antigens (HLA) class I and II expression on normal (n = 10), premalignant (n = 25), and mali...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/101.1.5

    authors: Hilders CG,Houbiers JG,Krul EJ,Fleuren GJ

    更新日期:1994-01-01 00:00:00

  • A new Mycobacterium species causing diffuse lepromatous leprosy.

    abstract::Mycobacterium leprae causes leprosy. M leprae strains collected worldwide have been genetically clonal, which poorly explains the varying severity and clinical features of the disease. We discovered a new Mycobacterium species from 2 patients who died of diffuse lepromatous leprosy (DLL). The Mycobacterium was purifie...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPP72FJZZRRVMM

    authors: Han XY,Seo YH,Sizer KC,Schoberle T,May GS,Spencer JS,Li W,Nair RG

    更新日期:2008-12-01 00:00:00

  • Correlation of pathologic features with clinical outcome in pediatric adrenocortical neoplasia. A study of a Brazilian population. Brazilian Group for Treatment of Childhood Adrenocortical Tumors.

    abstract::Although the relationship between pathologic features and clinical outcome is well established in adult adrenocortical neoplasms, the prognostic value of these features in pediatric adrenocortical neoplasms (PACN) is unclear. In a series of PACNs from 54 Brazilian children, the authors retrospectively investigated the...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/101.5.625

    authors: Bugg MF,Ribeiro RC,Roberson PK,Lloyd RV,Sandrini R,Silva JB,Epelman S,Shapiro DN,Parham DM

    更新日期:1994-05-01 00:00:00

  • Restricted kappa/lambda light chain ratio by flow cytometry in germinal center B cells in Hashimoto thyroiditis.

    abstract::To determine the diagnostic significance of the kappa/lambda ratio in germinal center (GC) B cells in Hashimoto thyroiditis (HT), we used 4-color flow cytometry to immunophenotype 27 samples (21 patients) of well-characterized HT B-cell clonality was analyzed further by polymerase chain reaction (PCR) of the immunoglo...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:

    authors: Chen HI,Akpolat I,Mody DR,Lopez-Terrada D,De Leon AP,Luo Y,Jorgensen J,Schwartz MR,Chang CC

    更新日期:2006-01-01 00:00:00

  • Serum enzyme alterations in polymyositis. Possible pitfalls in diagnosis.

    abstract::We report serum enzyme patterns in three patients with polymyositis or dermatomyositis whose cases posed potential problems in distinguishing malignancy, hepatocellular damage, and myocardial infarction from myositis. The alanine aminotransferase showed five- to 16-fold elevations. The creatine kinase MB isoenzyme and...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/73.4.556

    authors: Morton BD 3rd,Statland BE

    更新日期:1980-04-01 00:00:00

  • The stability of clinical chemistry specimens during refrigerated storage for 24 hours.

    abstract::The authors investigated the stability of the tests comprising the SMA-6 and SMA-12 chemistry profiles during overnight refrigerated storage. Several constituents were found to undergo changes that were statistically significant when evaluated by a t test for paired comparisons. A recently published sequential method ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/73.1.91

    authors: Rossing RG,Foster DM

    更新日期:1980-01-01 00:00:00

  • Comparison of bcr-abl protein expression and Philadelphia chromosome analyses in chronic myelogenous leukemia patients.

    abstract::The Philadelphia chromosome (Ph) is found in most chronic myelogenous leukemia (CML) patients. The bcr-abl oncoprotein (P210 or P185), the product of the fused bcr-abl gene produced by the Ph, is known to be the major factor in initiation and maintenance of the leukemic state in these types of leukemias. The authors h...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/106.4.442

    authors: Guo JQ,Lian J,Glassman A,Talpaz M,Kantarjian H,Deisseroth AB,Arlinghaus RB

    更新日期:1996-10-01 00:00:00

  • Rapid immunocytochemical analysis of acute leukemias.

    abstract::Immunophenotypic analysis of acute leukemias is time consuming and often requires flow cytometric analysis. A 1-hour alkaline phosphatase-labeled streptavidin-biotin immunocytochemical procedure was evaluated as an alternative. Seventeen cases of acute leukemia, including 10 acute lymphocytic (ALL) and 7 acute nonlymp...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/97.6.876

    authors: Hammer RD,Collins RD,Ebrahimi S,Casey TT

    更新日期:1992-06-01 00:00:00

  • An analysis of the relationship between a pathology department and its laboratory information system vendor.

    abstract::The ongoing collaborative relationship between a pathology department and the vendor of its laboratory information system is vitally important because of the strategic importance of the laboratory information system in managing the information product of the department. Laboratory information system vendors are value-...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/97.3.363

    authors: Friedman BA,Mitchell W

    更新日期:1992-03-01 00:00:00

  • An Audit of Repeat Testing at an Academic Medical Center: Consistency of Order Patterns With Recommendations and Potential Cost Savings.

    abstract:Objectives:To evaluate the prevalence of potentially unnecessary repeat testing (PURT) and the associated economic burden for an inpatient population at a large academic medical facility. Methods:We evaluated all inpatient test orders during 2016 for PURT by comparing the intertest times to published recommendations. ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/aqy020

    authors: Hueth KD,Jackson BR,Schmidt RL

    更新日期:2018-05-31 00:00:00

  • Estimation of PR and ER by immunocytochemistry in breast cancer. Comparison with radioligand binding methods.

    abstract::Immunocytochemical assays for progesterone receptor (PR) using monoclonal antirabbit PR antibodies (PR-ICA) and for estrogen receptor (ER) (ER-ICA) were compared with radioligand binding (dextran-coated charcoal [DCC]) methods for receptor determination in patients with breast cancer. Immunocytochemical staining for P...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:

    authors: Seymour L,Meyer K,Esser J,MacPhail AP,Behr A,Bezwoda WR

    更新日期:1990-10-01 00:00:00

  • The diagnostic utility of the minimal carcinoma triple stain in breast carcinomas.

    abstract::Pathologists are expected to accurately diagnose increasingly smaller breast carcinomas. Correct classification (ie, lobular vs ductal or in situ vs invasive) directly affects subsequent management, especially when the focus is near a surgical margin or present in a needle core biopsy and is further challenging if the...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPF4O0ADKFMGRJ

    authors: Ross DS,Liu YF,Pipa J,Shin SJ

    更新日期:2013-01-01 00:00:00

  • A hospital-wide outbreak of septicemia due to a few strains of Staphylococcus aureus.

    abstract::During a 6-month period at Walter Reed Army Hospital the monthly attack rate of Staphylococcus aureus bacteremia increased to 3.8 +/- 0.5 (mean +/- SEM) from 2.5 +/- 0.2 cases per 1,000 dispositions for the previous 48 months (P less than 0.05). A predominant phage pattern, designated S, was found in 12 (39%) of 31 ba...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/79.5.598

    authors: Cross AS,Zierdt CH,Roup B,Almazan R,Swan JC

    更新日期:1983-05-01 00:00:00

  • Pseudo-sulfur granules associated with intrauterine contraceptive devices.

    abstract::A structure resembling a sulfur granule found in histologic sections of endometrium from patients with intrauterine contraceptive devices is described. It is distinguished from a true sulfur granule on morphologic grounds and is further characterized as a foreign substance, most probably fragments of the synthetic con...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/75.6.822

    authors: O'Brien PK,Roth-Moyo LA,Davis BA

    更新日期:1981-06-01 00:00:00

  • Serum percent-free PSA does not predict extraprostatic spread of prostate cancer.

    abstract::Percent-free prostate-specific antigen (proportion of free prostate-specific antigen [PSA] to total PSA) has been shown recently in studies on frozen serum samples to be more useful than total PSA alone in distinguishing prostate cancer from benign conditions of the prostate gland. The primary purpose of our study was...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/109.5.533

    authors: Henricks WH,England BG,Giacherio DA,Oesterling JE,Wojno KJ

    更新日期:1998-05-01 00:00:00

  • Gleason Grading, Biochemical Failure, and Prostate Cancer-Specific Death.

    abstract:Objectives:To examine the relationship between the recently defined Gleason grade groups and prostate cancer-specific mortality. Methods:If the probability of prostate cancer-specific death is symbolized as P(PSD), the probability of biochemical failure is symbolized as P(BF), and the probability of prostate cancer-sp...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/aqw212

    authors: Vollmer RT

    更新日期:2017-03-01 00:00:00

  • Immunohistochemical characterization of renal amyloidosis.

    abstract::Forty-five renal biopsies with amyloidosis were studied by light microscopy with Congo red staining and action of potassium permanganate and by immunofluorescence with antihuman tissue A component antiserum antilight and heavy chains of immunoglobulins antisera. The patients were classified on the basis of concordance...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/87.6.756

    authors: Noel LH,Droz D,Ganeval D

    更新日期:1987-06-01 00:00:00

  • Nocardia asteroides bacteremia in an immunosuppressed renal-transplant patient.

    abstract::A patient with a polymicrobial bacteremia due to Nocardia asteroides and Escherichia coli is presented. Rapid overgrowth of all cultures with the E. coli necessitated the use of selective media containing antimicrobial agents to which the E. coli was sensitive. Only five previous cases of N. asteroides bacteremia have...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/64.4.537

    authors: Ruebush TK 2nd,Goodman JS

    更新日期:1975-10-01 00:00:00

  • Accurate Real-Time Diagnosis of Lymphoproliferative Disorders in Malawi Through Clinicopathologic Teleconferences:  A Model for Pathology Services in Sub-Saharan Africa.

    abstract:OBJECTIVES:The incidence of lymphoproliferative disorders (LPDs) is increasing in sub-Saharan Africa (SSA) due to population growth, aging, and human immunodeficiency virus (HIV). Despite significant burden, resources for diagnosis and treatment of LPDs are limited, with little infrastructure to deliver modern patholog...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/aqw118

    authors: Montgomery ND,Liomba NG,Kampani C,Krysiak R,Stanley CC,Tomoka T,Kamiza S,Dhungel BM,Gopal S,Fedoriw Y

    更新日期:2016-10-01 00:00:00

  • Quantitative Cytologic Descriptors to Differentiate CLL, Sézary, Granular, and Villous Lymphocytes Through Image Analysis.

    abstract:OBJECTIVES:We aimed to find descriptors to identify chronic lymphocytic leukemia (CLL), Sézary, granular, and villous lymphocytes among normal and abnormal lymphocytes in peripheral blood. METHODS:Image analysis was applied to 768 images from 15 different types of lymphoid cells and monocytes to determine four discrim...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/aqz025

    authors: Puigví L,Merino A,Alférez S,Boldú L,Acevedo A,Rodellar J

    更新日期:2019-06-05 00:00:00

  • The effects of heparin on lipoproteins in high-resolution electrophoresis of serum.

    abstract::Sera from heparinized patients commonly display anodal slurring of both the alpha and beta lipoproteins when they are examined by high-resolution electrophoresis (HRE). In this study, the authors examined the effect of heparin and lipoprotein lipase on the electrophoretic migration of alpha and beta lipoproteins in se...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/104.4.468

    authors: Pearson JP,Keren DF

    更新日期:1995-10-01 00:00:00

  • Profiles of black and Latin-American patients having pernicious anemia. HLA antigens, lymphocytotoxic antibody, anti-parietal cell antibody serum gastrin levels, and ABO blood groups.

    abstract::Because of the unusual presentation of pernicious anemia in many black women, a further characterization of this disease among the different racial groups was undertaken. The following were found. (1) Black women under the age of 50 years had a significantly lower prevalence of anti-parietal cell antibody than did "Eu...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/75.3.291

    authors: Carmel R,Ozturk G,Johnson CS,Tung KS,Terasaki PI

    更新日期:1981-03-01 00:00:00

  • Lack of in vitro interaction between heparin and nitroglycerin.

    abstract::Nitroglycerin has been reported to reduce activated partial thromboplastin time (aPTT) values in patients treated with concurrent heparin and nitroglycerin. However, in vivo studies have yielded conflicting results. In this in vitro evaluation, nitroglycerin was added to samples of pooled plasma from normal volunteers...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/105.3.298

    authors: Barnes AD,Horn JR,Wittkowsky AK

    更新日期:1996-03-01 00:00:00

  • The effects of tissue processing on markers for T and B cells from solid tissues.

    abstract::Suspensions of lymphoid cells from tissues have been used for the determination of the quantitative relationship between the T and B cell populations. The distribution of the lymphocytes within a given tissue, however, cannot be demonstrated once such a suspension has been prepared. Various methods of characterizing l...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/67.3.230

    authors: Millard PR,Rabin BS,Whiteside TL,Hubbard JD

    更新日期:1977-03-01 00:00:00