Abstract:
:Cytologic examination of peritoneal lavage fluid is a useful predictor of peritoneal recurrence in gastric cancer. However, this technique is not overly sensitive and requires special abilities in the cytologist. In this study, telomerase activity was used to detect free cancer cells in peritoneal lavage fluid from patients with gastric cancer. In the first part, 12 lavage-fluid samples obtained from 12 patients with gastric cancer were analysed using the conventional telomeric repeat amplification protocol (TRAP) assay. Three of five patients with early gastric cancer had positive telomerase activity. These false-positive results may have been due to lymphocyte contamination. Furthermore, polymerase chain reaction inhibitors were also detected in the lavage-fluid samples. Therefore, we developed a novel method for elimination of haematopoietic cell and Taq polymerase inhibitors to increase the accuracy of the TRAP assay using immunomagnetic beads, which bind to most normal and neoplastic human epithelial cells. Telomerase activity was found in 10 of 20 (50%) lavage-fluid samples from patients with serosal or subserosal invasion. Cytologic examination was positive in nine of 20 (45%) samples. Both the telomerase activity and cytology were negative in all 14 patients without serosal or subserosal invasion. These results suggest that the TRAP assay combined with immunomagnetic beads might be useful for detection of free cancer cells in the peritoneal space in gastric cancer without the aid of an experienced cytologist.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Mori N,Oka M,Hazama S,Iizuka N,Yamamoto K,Yoshino S,Tangoku A,Noma T,Hirose Kdoi
10.1054/bjoc.2000.1408subject
Has Abstractpub_date
2000-10-01 00:00:00pages
1026-32issue
8eissn
0007-0920issn
1532-1827pii
S0007092000914083journal_volume
83pub_type
杂志文章abstract::Associations between work in the electrical and electronic industry and cancer incidence were assessed using data for 371 890 cancers registered in England between 1981 and 1987, of which 7981 were in electrical workers. Proportional registration ratios (PRRs) were calculated, both with and without the commonest cance...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1996.167
更新日期:1996-04-01 00:00:00
abstract::Tamoxifen is the current therapy of choice for patients with oestrogen receptor positive breast cancer, and it is currently under evaluation as a prophylactic for women at high risk of developing the disease. However, tamoxifen is also known to induce proliferative changes in the endometrium increasing the risk of dev...
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journal_title:British journal of cancer
pub_type: 杂志文章
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journal_title:British journal of cancer
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更新日期:2020-02-01 00:00:00
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更新日期:2016-05-10 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1038/sj.bjc.6690108
更新日期:1999-02-01 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章,多中心研究,随机对照试验
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更新日期:2018-03-20 00:00:00
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doi:10.1038/sj.bjc.6602744
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pub_type: 临床试验,杂志文章,随机对照试验
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6690601
更新日期:1999-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1038/bjc.1980.203
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更新日期:2011-03-15 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2019-01-01 00:00:00
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pub_type: 杂志文章,随机对照试验
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1981.26
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