Abstract:
:Telomerase, an enzyme related with cellular immortality, has been extensively studied in many kinds of malignant tumours for clinical diagnostic or prognostic utilities. Telomerase activity is mainly regulated by the expression of hTERT (human telomerase reverse transcriptase), which is a catalytic component of human telomerase. To evaluate whether the levels of hTERT mRNA provides a molecular marker of hepatoblastoma malignancy, we examined hTERT mRNA expression levels in the primary hepatoblastoma tissues by fluorescent RT-PCR using LightCycler technology and followed up the clinical outcomes in 63 patients listed in the Japanese Study Group of Pediatric Liver Tumor between 1991 and 2002. The hTERT mRNA expression was detected in 61 (96.8%) specimens and their expression levels ranged between 0.1/1000 and 745.1/1000 copies of PBGD gene that was used as an internal control. Among these cases, frozen 39 tumour samples and 14 adjacent noncancerous liver tissues were analysed for semiquantitative telomerase assay. In the 39 tumour samples, the levels of telomerase activity ranged between 0.11 and 2709 TPG and 12 (30.7%) had high telomerase activity (>100 TPG), whereas only nine of 14 noncancerous liver tissue samples showed telomerase activity which was less than 1.0 TPG. The levels of telomerase activity were significantly correlated with the levels of hTERT mRNA expression (P<0.001). The frequency of high hTERT mRNA expression and/or high telomerase activity did not significantly associate with the clinicopathological factors except for stage of disease. The prognosis of the patients with high hTERT mRNA expression was significantly worse than that of others (P<0.01), as was the patients with high telomerase activity (P<0.01). Multivariate analysis indicated that high levels of hTERT mRNA expression as well as telomerase activity are independent prognosis-predicting factors in patients with hepatoblastoma.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Hiyama E,Yamaoka H,Matsunaga T,Hayashi Y,Ando H,Suita S,Horie H,Kaneko M,Sasaki F,Hashizume K,Nakagawara A,Ohnuma N,Yokoyama Tdoi
10.1038/sj.bjc.6602054subject
Has Abstractpub_date
2004-08-31 00:00:00pages
972-9issue
5eissn
0007-0920issn
1532-1827pii
6602054journal_volume
91pub_type
杂志文章abstract::In vitro studies identified three Burkitts lymphoma cell lines, Ramos, MUTU-I and Daudi, that were growth inhibited by anti-IgM antibody. However, only Ramos and MUTU-I were sensitive to monoclonal antibodies (mAb) recognizing the Fc region of surface IgM (anti-Fc mu). Experiments using anti-Fc mu mAb (single or non-c...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6690136
更新日期:1999-02-01 00:00:00
abstract::People affected by cancer want information about their prognosis but clinicians have trouble estimating and talking about it. We sought to determine the nature and accuracy of medical oncologists' estimates of life expectancy in newly referred patients with incurable cancer. With reference to each patient, medical onc...
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doi:10.1038/sj.bjc.6602908
更新日期:2006-01-30 00:00:00
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journal_title:British journal of cancer
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doi:10.1038/bjc.1998.549
更新日期:1998-09-01 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章,多中心研究,随机对照试验
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更新日期:2006-10-09 00:00:00
abstract:BACKGROUND:Activated anaplastic lymphoma kinase (ALK) gene fusions are recurrent events in a small fraction of colorectal cancers (CRCs), although these events have not yet been exploited as in other malignancies. METHODS:We detected ALK protein expression by immunohistochemistry and gene rearrangements by fluorescenc...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2015.401
更新日期:2015-12-22 00:00:00
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doi:10.1038/bjc.2012.563
更新日期:2013-02-05 00:00:00
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pub_type: 杂志文章
doi:10.1038/bjc.1998.89
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1994.136
更新日期:1994-04-01 00:00:00
abstract::The combination of 5-fluorouracil (5-FU) and interferon-alpha (IFN-alpha) has reported activity in the treatment of advanced colorectal carcinoma. Laboratory studies of IFN-beta suggest that this agent may offer theoretical advantages over IFN-alpha in combination with 5-FU. A total of 27 patients with advanced or rec...
journal_title:British journal of cancer
pub_type: 临床试验,杂志文章
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journal_title:British journal of cancer
pub_type: 杂志文章,meta分析,多中心研究
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pub_type: 杂志文章
doi:10.1038/bjc.2011.276
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1976.186
更新日期:1976-10-01 00:00:00
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更新日期:1998-12-01 00:00:00
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journal_title:British journal of cancer
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journal_title:British journal of cancer
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doi:10.1038/bjc.1979.228
更新日期:1979-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1038/sj.bjc.6601578
更新日期:2004-01-26 00:00:00
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pub_type: 杂志文章
doi:10.1038/sj.bjc.6602161
更新日期:2004-11-01 00:00:00
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pub_type: 临床试验,杂志文章
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journal_title:British journal of cancer
pub_type: 杂志文章
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更新日期:1995-05-01 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6690173
更新日期:1999-03-01 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1993.17
更新日期:1993-01-01 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
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更新日期:2011-03-15 00:00:00
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