Evidence that estrogens directly alter androgen-regulated prostate development.


:Neonatal exposure to high doses of estrogen results in permanent suppression of prostate growth and reduced sensitivity to androgens in adulthood. It is unclear whether alterations in prostate growth are due to a direct effect of estrogens on the gland or are the result of hypothalamic-pituitary-gonadal axis suppression and a subsequent reduction in androgen levels. Therefore, the aim of this study was to determine whether estrogens have a direct effect on the prostate using a defined method of culturing neonatal prostates. Newborn rat ventral prostates were microdissected and cultured in the presence of testosterone, which resulted in branching morphogenesis and ductal canalization. Solid cords of epithelium differentiated into acini lined by tall columnar epithelial cells; these acini were surrounded by stromal cells, expressing smooth muscle alpha-actin. When cultured in the presence of 17beta-estradiol or diethylstilbestrol in addition to testosterone, androgen-induced prostatic growth was reduced, and differentiation was altered. Although estrogen-treated explants were smaller than controls, quantification of epithelial, stromal, and luminal volumes using unbiased stereology revealed significant changes; the proportion of epithelial cells and lumen decreased, and the proportion of stroma increased compared with control values. Concurrent with this reduced growth rate, we observed a disturbance in the branching pattern and a reduction in ductal canalization. Specifically, stromal differentiation and organization were disrupted, so that a discontinuous smooth muscle layer was observed around the epithelial ducts, and epithelial differentiation was altered. The effects of estrogens were not accompanied by a decrease in androgen response via the androgen receptor, because immunolocalization of this receptor remained constant. These data demonstrate that high doses of estrogens are growth inhibitory and have direct effects on prostate development in vitro, which may occur in vivo in addition to indirect effects via suppression of the hypothalamic-pituitary-gonadal axis.






Jarred RA,Cancilla B,Prins GS,Thayer KA,Cunha GR,Risbridger GP




Has Abstract


2000-09-01 00:00:00












  • Wingless (Wnt)-3A induces trophoblast migration and matrix metalloproteinase-2 secretion through canonical Wnt signaling and protein kinase B/AKT activation.

    abstract::Invasion of human trophoblasts is promoted through activation of wingless (Wnt) signaling, suggesting a role of the pathway in placental development and morphogenesis. However, details on the process such as involvement of canonical and/or noncanonical Wnt signaling cascades as well as their target genes are largely u...


    pub_type: 杂志文章


    authors: Sonderegger S,Haslinger P,Sabri A,Leisser C,Otten JV,Fiala C,Knöfler M

    更新日期:2010-01-01 00:00:00

  • Regulation of estrogen and progesterone receptor levels in mouse mammary epithelial cells grown in serum-free collagen gel cultures.

    abstract::The effect of collagenase dissociation of virgin mouse mammary glands on the level of mammary epithelial cytosolic estrogen receptors (ER) and progesterone receptors (PR) was assessed. After cell dissociation, ER was present in mammary epithelial cells at concentrations similar to those found in the whole gland. Howev...


    pub_type: 杂志文章


    authors: Edery M,Imagawa W,Larson L,Nandi S

    更新日期:1985-01-01 00:00:00

  • Role of the hypophysis in regulation of pancreatic and gastric somatostatin.

    abstract::Since hypophysectomy and GH deficiency are associated with decreases in hypothalamic content and release of SRIF, it was of interest to determine whether these hormonal alterations also affect peripheral tissue levels of SRIF. Hypophysectomized (hypox) rats were studied at various times after surgery and compared with...


    pub_type: 杂志文章


    authors: Recant L,Voyles NR,Wilkins SD,Fairtile RD,King C

    更新日期:1984-12-01 00:00:00

  • Effect of estrogen on adenosine 3'5',cyclic monophosphate in quail oviduct: possible involvement in estradiol-activated growth.

    abstract::Previous studies have shown that estradiol indirectly stimulated the proliferation of oviduct epithelial cells in the quail. The present study was undertaken to investigate the effects of estradiol and other steroid hormones on the cAMP concentration. The ability of forskolin, a specific activator of the catalytic sub...


    pub_type: 杂志文章


    authors: Laugier C,Courion C,Pageaux JF,Fanidi A,Dumas MY,Sandoz D,Nemoz G,Prigent AF,Pacheco H

    更新日期:1988-01-01 00:00:00

  • Regulation of Na-K-Cl cotransport, Na,K-adenosine triphosphatase, and Na/H exchanger in human neuroblastoma NB-OK-1 cells by atrial natriuretic peptide.

    abstract::The occupancy of atrial natriuretic peptide (ANP) receptors of the ANPA type in human neuroblastoma NB-OK-1 cells elevates cGMP. In this study, ANP concentrations of 10 nM or more increased total K+ uptake. Data obtained in the presence of bumetanide and/or ouabain demonstrated that 1 microM ANP induced a primary stim...


    pub_type: 杂志文章


    authors: Delporte C,Winand J,Poloczek P,Christophe J

    更新日期:1993-07-01 00:00:00

  • Alcohol and fatty acid stimulation of neurotensin release from rat small intestine.

    abstract::We have previously reported that neurotensin (NT) is released from the small intestine and elevated in the hepatic-portal circulation in response to the perfusion of the small intestine with a micellar solution of oleic acid. In order to determine the minimum acyl chain length and whether the presence of a carboxylic ...


    pub_type: 杂志文章


    authors: Ferris CF,Armstrong MJ,George JK,Stevens CA,Carraway RE,Leeman SE

    更新日期:1985-03-01 00:00:00

  • Interleukin-6 is an essential determinant of on-time parturition in the mouse.

    abstract::IL-6 abundance in amniotic fluid and uterine tissues increases in late gestation or with infection-associated preterm labor. A role in regulation of labor onset is suggested by observations that IL-6 increases expression of genes controlling prostaglandin synthesis and signaling in isolated uterine cells, but whether ...


    pub_type: 杂志文章


    authors: Robertson SA,Christiaens I,Dorian CL,Zaragoza DB,Care AS,Banks AM,Olson DM

    更新日期:2010-08-01 00:00:00

  • Localization and measurement of corticostatin-I in nonpregnant and pregnant rabbit tissues during late gestation.

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    pub_type: 杂志文章


    authors: Hu J,Jothy S,Solomon S

    更新日期:1993-06-01 00:00:00

  • GLP-2 Dysregulates Hepatic Lipoprotein Metabolism, Inducing Fatty Liver and VLDL Overproduction in Male Hamsters and Mice.

    abstract::Fundamental complications of insulin resistance and type 2 diabetes include the development of nonalcoholic fatty liver disease and an atherogenic fasting dyslipidemic profile, primarily due to increases in hepatic very-low-density lipoprotein (VLDL) production. Recently, central glucagon-like peptide-2 receptor (GLP2...


    pub_type: 杂志文章


    authors: Taher J,Baker C,Alvares D,Ijaz L,Hussain M,Adeli K

    更新日期:2018-09-01 00:00:00

  • Autoimmune oophoritis with multiple molecular targets mitigated by transgenic expression of mater.

    abstract::Primary ovarian insufficiency (POI) resulting from ovarian autoimmunity is a poorly understood clinical condition lacking in effective treatments. Understanding the targets of the autoimmune response and induction of ovarian-specific tolerance would allow development of focused therapies to preserve fertility in an at...


    pub_type: 杂志文章


    authors: Otsuka N,Tong ZB,Vanevski K,Tu W,Cheng MH,Nelson LM

    更新日期:2011-06-01 00:00:00

  • In vitro evidence that an 11-kilodalton N-terminal fragment of proopiomelanocortin is a growth factor specifically stimulating the development of lactotrophs in rat pituitary during postnatal life.

    abstract::We have previously shown that in reaggregate cell cultures of 14-day-old female rat pituitary, LHRH is capable of stimulating lactotrophs to enter the S-phase of the cell cycle, and that this effect is mediated by paracrine growth factors secreted from gonadotrophs. In the present report we describe the isolation, pur...


    pub_type: 杂志文章


    authors: Tilemans D,Andries M,Proost P,Devreese B,Van Beeumen J,Denef C

    更新日期:1994-07-01 00:00:00

  • Lysophosphatidic acid inhibits C-type natriuretic peptide activation of guanylyl cyclase-B.

    abstract::C-type natriuretic peptide (CNP), found in endothelial cells, chondrocytes, and neurons, binds its cognate transmembrane receptor, natriuretic peptide receptor-B (NPR-B/GC-B), and stimulates the synthesis of the intracellular signaling molecule, cGMP. The known physiologic consequences of this binding event are vasore...


    pub_type: 杂志文章


    authors: Abbey SE,Potter LR

    更新日期:2003-01-01 00:00:00

  • Effects of insulin on ribonucleic acid synthesis in toad urinary bladder.

    abstract::We previously found that the sustained rise in active sodium transport by the toad urinary bladder after exposure to insulin is associated with the synthesis of a membrane protein with a molecular weight of 25,000. Studying the effects of insulin on the incorporation of uridine into RNA of epithelial cells of the blad...


    pub_type: 杂志文章


    authors: Cobb MH,Yang CP,Scott WN

    更新日期:1981-12-01 00:00:00

  • Identification by photoaffinity labeling of a membrane thyroid hormone-binding protein associated with the triiodothyronine transport system in rat erythrocytes.

    abstract::Photoaffinity labeling with underivatized T3 was used to identify T3-binding proteins in the membrane of rat erythrocytes. UV irradiation of ghosts and peripheral protein-depleted membranes in the presence of [125I]T3 resulted in the covalent attachment of 125I to membrane proteins (analyzed by polyacrylamide gel elec...


    pub_type: 杂志文章


    authors: Samson M,Osty J,Blondeau JP

    更新日期:1993-06-01 00:00:00

  • Processing of human growth hormone by rat hepatocytes in monolayer culture.

    abstract::The fate of cell-bound [125I]iodo-human GH ([125I]iodo-hGH) was studied in monolayer cultures of hepatocytes from pregnant and nonpregnant rats. Both the binding of [125I]iodo-hGH and its degradation were significantly higher in cells from pregnant than from nonpregnant rats. The positive correlation between the numbe...


    pub_type: 杂志文章


    authors: Wakai K,Tsushima T,Isozaki O,Sato Y,Sato K,Shizume K

    更新日期:1984-05-01 00:00:00

  • Cessation of long day melatonin rhythms time puberty in a short day breeder.

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    pub_type: 杂志文章


    authors: Foster DL,Ebling FJ,Claypool LE,Woodfill CJ

    更新日期:1988-09-01 00:00:00

  • The role of calcium/phospholipid-dependent protein kinase in Leydig cell steroidogenesis.

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    pub_type: 杂志文章


    authors: Lin T

    更新日期:1985-07-01 00:00:00

  • Production of the rat type 1 insulin-like growth factor-binding protein by well differentiated H4EIIC3 hepatoma cells: identification, purification, and N-terminal amino acid analysis.

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    pub_type: 杂志文章


    authors: Unterman TG,Oehler DT,Gotway MB,Morris PW

    更新日期:1990-08-01 00:00:00

  • Aromatase cytochrome P450 and cholesterol side-chain cleavage cytochrome P450 in corpora lutea of pregnant rats: diverse regulation by peptide and steroid hormones.

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    pub_type: 杂志文章


    authors: Hickey GJ,Oonk RB,Hall PF,Richards JS

    更新日期:1989-09-01 00:00:00

  • Maximal testosterone production in Leydig cells from inbred mice relates to the activity of 3 beta-hydroxysteroid dehydrogenase-isomerase.

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    pub_type: 杂志文章


    authors: Stalvey JR,Payne AH

    更新日期:1984-10-01 00:00:00

  • Failure of growth hormone (GH) to feed back at the level of the pituitary to alter the response of the somatotrophs to GH-releasing factor.

    abstract::GH feeds back at the level of the central nervous system to alter the release of somatostatin and GRF, resulting in altered GH release. The purpose of this study was to see whether the concentration of GH, impinging directly on the somatotrophs of the adenohypophysis, would alter the responsiveness of the somatotrophs...


    pub_type: 杂志文章


    authors: Kraicer J,Lussier B,Moor BC,Cowan JS

    更新日期:1988-04-01 00:00:00

  • The effect of lithium on glucose- and tolbutamide-induced insulin release and glucose tolerance in the intact rat.

    abstract::The effects of lithium on stimulus-induced insulin release and glucose tolerance were examined in intact, unanesthetized, and unrestrained rats with indwelling intravascular catheters. In the first study, a 150-mg iv glucose pulse was administered at 30 min after rapid iv injection of lithium carbonate (1 meq/kg) or v...


    pub_type: 杂志文章


    authors: Shah JH,Pishdad G

    更新日期:1980-11-01 00:00:00

  • Estrogen feedback needed at the paraventricular nucleus or A2 to suppress pulsatile luteinizing hormone release in fasting female rats.

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    pub_type: 杂志文章


    authors: Nagatani S,Tsukamura H,Maeda K

    更新日期:1994-09-01 00:00:00

  • Sertoli Cell Number Defines and Predicts Germ and Leydig Cell Population Sizes in the Adult Mouse Testis.

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    pub_type: 杂志文章


    authors: Rebourcet D,Darbey A,Monteiro A,Soffientini U,Tsai YT,Handel I,Pitetti JL,Nef S,Smith LB,O'Shaughnessy PJ

    更新日期:2017-09-01 00:00:00

  • α7-Nicotinic Acetylcholine Receptor Agonist Ameliorates Nicotine Plus High-Fat Diet-Induced Hepatic Steatosis in Male Mice by Inhibiting Oxidative Stress and Stimulating AMPK Signaling.

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    pub_type: 杂志文章


    authors: Hasan MK,Friedman TC,Sims C,Lee DL,Espinoza-Derout J,Ume A,Chalfant V,Lee ML,Sinha-Hikim I,Lutfy K,Liu Y,Mahata SK,Sinha-Hikim AP

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    pub_type: 杂志文章


    authors: Muramami N,Fukata J,Tsukada T,Kobayashi H,Ebisui O,Segawa H,Muro S,Imura H,Nakao K

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    pub_type: 杂志文章


    authors: Abe Y,Cooper E,Spaulding SW

    更新日期:1982-06-01 00:00:00

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    pub_type: 杂志文章


    authors: Toaff ME,Schleyer H,Strauss JF 3rd

    更新日期:1982-12-01 00:00:00

  • Effects of free fatty acids on luteinizing hormone and growth hormone secretion in ovariectomized lambs.

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    pub_type: 杂志文章


    authors: Estienne MJ,Schillo KK,Hileman SM,Green MA,Hayes SH,Boling JA

    更新日期:1990-04-01 00:00:00

  • Creation of estrogen resistance in vivo by transgenic overexpression of the heterogeneous nuclear ribonucleoprotein-related estrogen response element binding protein.

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    pub_type: 杂志文章


    authors: Chen H,Stuart W,Hu B,Nguyen L,Huang G,Clemens TL,Adams JS

    更新日期:2005-10-01 00:00:00