Abstract：
:Inhalation of swine dust causes intense airway inflammation with a multifold increase of inflammatory cells and lymphocyte activation as assessed by bronchoalveolar lavage. To further investigate the mechanism for lymphocyte activation the present in vitro study focuses on the lymphocyte response to swine dust in whole blood. Various concentrations of phytohaemagglutinin (PHA) (final concentrations: 3.16, 10.0, 3.16 and 100 microg ml(-1)) and swine dust (final concentrations: 10.0, 31.6, 100 and 316 microg ml(-1)) were added to heparinized whole blood from healthy donors. The blood samples were incubated in duplicate, using the homologous unstimulated blood as control, for 4, 24, 48 and 72 h in a water bath at 37 degrees C. The cells were stained with fluorochrome conjugated monoclonal antibodies. For analysis of T-cell activation CD3 was double-stained together with the activation markers CD69, CD25 and HLADR. Cell count percentages were analysed by flow cytometry. Soluble IL-2sRalpha in plasma was analysed using commercial sandwich ELISA technique. At baseline CD69, CD25 and HLA-DR were expressed in < 1%, approx 5% and < 1% of the T-cells respectively. We found a dose response relationship between swine dust exposure and the expression of all three T-cell activation markers which appeared at different time-points. Maximal expression of CD69 (8%, P<0.05) and CD25 (15%, P<0.001) was found after 24h of activation. HLA-DR was significantly expressed after 48h (8%) and maximally expressed after 72 h of activation (13%, P<0.05). The soluble IL-2sRalpha in plasma was maximally expressed after 24-48 h (1200 pg ml(1) and 1500 pg ml(-1), respectively. In conclusion, T-cells were activated by swine dust in vitro. Thus, our previous findings of T-cell activation following swine dust exposure, in vivo may be an effect of the dust either directly on T-cells or on other cells which in turn contribute to the T-cell activation.
journal_name
Respir Medjournal_title
Respiratory medicineauthors
Müller-Suur C,Larsson PH,Larsson Kdoi
10.1053/rmed.2000.0828subject
Has Abstractpub_date
2000-08-01 00:00:00pages
821-7issue
8eissn
0954-6111issn
1532-3064pii
S0954-6111(00)90828-Xjournal_volume
94pub_type
杂志文章abstract：BACKGROUND:Gas6 protein is involved in pulmonary embolism (PE) and acute inflammation in animal models. METHODS:We enrolled 82 consecutive patients with acute dyspnea and suspected PE (Geneva score with high (HCP) or low/intermediate clinical probability (LICP)+D-dimer >or=0.5microg/mL) and 29 age-matched healthy volu...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/j.rmed.2008.10.018
更新日期：2009-04-01 00:00:00
abstract：:The results of pulmonary function testing with a turbine spirometer (TS) and a bell spirometer (BS) of a randomized group of 275 patients aged 4-18 years were compared. In the TS, an inexpensive device without graphical display was used. The difference BS minus TS (d) for forced expiratory volume in 1 s (FEV1) was cal...
journal_title：Respiratory medicine
pub_type： 临床试验,杂志文章
doi：10.1016/s0954-6111(97)90064-0
更新日期：1997-07-01 00:00:00
abstract：:Although pleural manometry is a relatively simple medical procedure it is only occasionally used to follow pleural pressure (Ppl) changes during a therapeutic thoracentesis and pneumothorax drainage. As some studies showed that pleural pressure monitoring might be associated with significant advantages, pleural manome...
journal_title：Respiratory medicine
pub_type： 杂志文章,评审
doi：10.1016/j.rmed.2018.01.014
更新日期：2018-12-01 00:00:00
abstract：INTRODUCTION:Primary ciliary dyskinesia (PCD) is a rare genetically heterogeneous disorder of motile cilia, which leads to recurrent and chronic airway infections. Detailed information about infection causing pathogens is scarce. With this study, we aimed to determine the prevalence and susceptibility of the most commo...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/j.rmed.2019.08.009
更新日期：2019-09-01 00:00:00
abstract：BACKGROUND:Patients with idiopathic pulmonary fibrosis (IPF) present an important ventilatory limitation reducing their exercise capacity. Non-invasive ventilatory support has been shown to improve exercise capacity in patients with obstructive diseases; however, its effect on IPF patients remains unknown. OBJECTIVE:T...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/j.rmed.2009.08.001
更新日期：2010-01-01 00:00:00
abstract：:Specific targeting of patients with a previous asthma hospitalisation could be more focused if predictors could be identified. This study was an observational retrospective analysis using ridge and linear multivariate regression analysis. Patient asthma management data were extracted from the hospital and general prac...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/j.rmed.2004.11.003
更新日期：2005-06-01 00:00:00
abstract：BACKGROUND:Pneumonia is a leading cause of morbidity and mortality in children worldwide; however, its diagnosis can be challenging, especially in settings where skilled clinicians or standard imaging are unavailable. We sought to determine the diagnostic accuracy of lung ultrasound when compared to radiographically-co...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/j.rmed.2017.05.007
更新日期：2017-07-01 00:00:00
abstract：INTRODUCTION:The aim was to investigate temporal variation in Health-Related Quality of Life (HRQL) and factors influencing low HRQL, in patients with asthma. MATERIAL AND METHODS:Questionnaire data on patient characteristics and the mini-Asthma Quality of Life Questionnaire (mini-AQLQ) scores from two separate cohort...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/j.rmed.2017.10.010
更新日期：2017-11-01 00:00:00
abstract：:Chlorofluorocarbon (CFC)-containing inhalers for use in the treatment of asthma are to be phased out under the terms of the Montreal Protocol (1). In this multi-centre, randomized, double-blind study, the therapeutic equivalence of two formulations of beclomethasone dipropionate (BDP) containing CFC or non-CFC (HFA134...
journal_title：Respiratory medicine
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1053/rmed.1999.0678
更新日期：2000-01-01 00:00:00
abstract：:The diagnostic value of isocapnic hyperventilation of cold air (IHCA) is not fully established. All 342 adult patients in whom IHCA had been performed because of a clinical suspicion of asthma between 1992 and 1994 were analysed retrospectively in the authors' hospital. In addition, 26 healthy subjects were recruited....
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/s0954-6111(97)90112-8
更新日期：1997-09-01 00:00:00
abstract：BACKGROUND:Genes that contribute to the risk of developing Chronic Obstructive Pulmonary Disease (COPD) have been identified, but an attempt to accurately quantify the total genetic contribution to COPD has to our knowledge never been conducted. METHODS:Hospital discharge diagnoses data on COPD were analysed in 22,422...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/j.rmed.2010.05.004
更新日期：2010-12-01 00:00:00
abstract：BACKGROUND:Anxiety and depression are prevalent in patients with asthma, and associated with more exacerbations and increased health care utilization. Since psychiatric intervention might improve asthma control, we examined whether patients with severe, prednisone-dependent asthma are at higher risk of these disorders ...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/j.rmed.2013.12.012
更新日期：2014-03-01 00:00:00
abstract：:This manuscript offers an approach to the treatment of pulmonary sarcoidosis based on current available information. The treatment of pulmonary sarcoidosis is not mandatory as the disease may be self-limiting and therapy is often associated with significant drug side effects. The decision to treat rests predominantly ...
journal_title：Respiratory medicine
pub_type： 杂志文章,评审
doi：10.1016/j.rmed.2012.01.013
更新日期：2012-10-01 00:00:00
abstract：:Chronic obstructive pulmonary disease (COPD) is now the most common indication for single lung transplantation. In long-term follow-up, obliterative bronchiolitis is a major problem. The aim of the present study was to perform a long-term follow-up of the pulmonary function and to examine the effect of development of ...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/s0954-6111(96)90148-1
更新日期：1996-10-01 00:00:00
abstract：BACKGROUND:Exhaled breath condensate (EBC) is simple to collect and as such a non-invasive method that has attracted substantial interest in the last few years. However, several methodological concerns have been raised and it has been difficult to reproduce results between different centres. Because of low concentratio...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/j.rmed.2005.04.007
更新日期：2006-01-01 00:00:00
abstract：:Behçet's disease (BD) is a multisystemic disease that may involve all systems, the most common symptoms being oral and genital ulcerations and ocular involvement. Pulmonary involvement is not usually investigated in BD unless there is a specific complaint. In this study, pulmonary parameters and findings were investig...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/j.rmed.2012.04.005
更新日期：2013-03-01 00:00:00
abstract：:Long-term oxygen therapy (LTOT) in patients with chronic hypoxaemia is an expanding field. Traditional stationary oxygen, with and without portable compressed gas cylinders, and portable liquid oxygen equipment is available today. No official guidelines exist for the prescription of liquid oxygen vs traditional oxygen...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/s0954-6111(98)90037-3
更新日期：1998-01-01 00:00:00
abstract：:We studied the effects of chronic nocturnal hypoxaemia due to obstructive sleep apnoea syndrome (OSAS) on the hypothalamic-pituitary-thyroid and hypothalamic-pituitary-testicular axes and on catecholamine and cortisol secretion. We investigated whether hormones other than catecholamines may serve as markers for chroni...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/s0954-6111(99)90068-9
更新日期：1999-01-01 00:00:00
abstract：:Antigen detection is a useful adjunct for the diagnosis of histoplasmosis. The purpose of this study was to evaluate antigen detection in bronchoalveolar lavage (BAL) fluid using an improved second-generation Histoplasma antigen assay. Antigen was detected in 16 of 19 (84%) cases of histoplasmosis and 5 of 6 (83.3%) b...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/j.rmed.2006.04.017
更新日期：2007-01-01 00:00:00
abstract：:Patients with congestive heart failure and Cheyne-Stokes respiration have a low arterial oxygen saturation, especially during sleep, which can be increased by breathing oxygen. Chronic alterations in blood gases are known to modulate the hypercapnic ventilatory response (HCVR). We therefore evaluated whether the HCVR ...
journal_title：Respiratory medicine
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0954-6111(98)90286-4
更新日期：1998-03-01 00:00:00
abstract：RATIONALE:COPD is a debilitating disease with increasing mortality worldwide. The BODE index evaluates disease severity and the St George's Respiratory Questionnaire (SGRQ) measures health status. OBJECTIVE:To identify the relationship between BODE index and the SGRQ and to test the predictive value of both tools agai...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/j.rmed.2011.01.007
更新日期：2011-06-01 00:00:00
abstract：OBJECTIVES:To evaluate the relationship between cephalometric parameters, upper airway morphological factors and obstructive sleep apnea (OSA) in Chinese subjects. DESIGN:Polysomnogram (PSG) were performed and scored using standard criteria. Supine lateral cephalometric parameters and pharyngeal cross-sectional areas ...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/j.rmed.2003.10.009
更新日期：2004-04-01 00:00:00
abstract：:The prevalence of tuberculosis in small solitary lesions of the lung obtained by video-assisted thoracoscopic surgery (VATS) is still unclear. Of 103 lung lesions resected by VATS in 98 patients (47 men, 51 women), 19 were identified macroscopically as inflammatory changes, 78 were neoplastic, and 6 were undefined. Pr...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/j.rmed.2005.05.004
更新日期：2006-02-01 00:00:00
abstract：:There is no agreement on the minimum absolute eosinophil count essential for the diagnosis of tropical pulmonary eosinophilia (TPE) at present. The aim of this study was to determine this figure as well as to evaluate the other diagnostic criteria of TPE. The response to diethylcarbamazine (DEC) was tested in 98 patie...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/s0954-6111(99)90106-3
更新日期：1999-09-01 00:00:00
abstract：OBJECTIVE:Small fiber neuropathy commonly affects patients with sarcoidosis and is often refractory to standard immunosuppressive therapies used for systemic disease. The clinical features of sarcoidosis-associated small fiber neuropathy (SSFN) and its response to medical therapy have not been described in a large popu...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/j.rmed.2017.03.011
更新日期：2017-05-01 00:00:00
abstract：BACKGROUND:Spirometry data predict mortality, but are less sensitive to detect dysfunction in small airways as compared to the slope of phase III (the N2 slope) of the single breath nitrogen test. The association between the N2 slope and mortality has been studied with conflicting results. In the present study the prog...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/j.rmed.2016.01.002
更新日期：2016-03-01 00:00:00
abstract：BACKGROUND:The aim of the present study was to estimate the societal costs and the key cost drivers for patients with severe asthma in Sweden. In addition, health-related quality of life (HRQOL) and morbidity of patients with severe asthma is described. METHODS:The study population comprised adults with severe asthma ...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/j.rmed.2019.105860
更新日期：2020-02-01 00:00:00
abstract：BACKGROUND:The efficacy and safety of extrafine beclomethasone dipropionate 100 μg/formoterol 6 μg (BDP/F HFA) pressurized metered dose inhaler (pMDI) in patients with moderate-to-severe persistent asthma, has been demonstrated in randomised controlled trials (RCTs). The aim of this prospective observational study was ...
journal_title：Respiratory medicine
pub_type： 杂志文章,多中心研究
doi：10.1016/j.rmed.2012.01.010
更新日期：2012-06-01 00:00:00
abstract：BACKGROUND:A predominant feature of asthma is an accelerated rate of decline in forced expiratory volume in 1 s (FEV1), but data on the variability and factors associated with this change in patients with controlled asthma are largely unknown. METHODS:140 patients with controlled asthma were enrolled based on the Glob...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/j.rmed.2014.04.014
更新日期：2014-07-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Mycobacterium tuberculosis has been proposed as a candidate agent for the cause of sarcoidosis. The QuantiFERON-TB Gold test has a higher specificity for detecting M. tuberculosis infection than the conventional tuberculin skin test. This study aimed to investigate the rate of positive QuantiFE...
journal_title：Respiratory medicine
pub_type： 杂志文章
doi：10.1016/j.rmed.2007.09.013
更新日期：2008-02-01 00:00:00
