Abstract:
:In the present study, we have investigated the effect of cytotropic heterogeneous molecular lipid (CHML), a new anticancer agent, on growth suppression in a variety of human tumor cell lines. At a non-toxic concentration (a range from 25 micrograms/ml to 100 micrograms/ml), CHML has shown to strongly inhibit tumor cell growth by using a typical colony survival assay. At a treatment of concentration of 50 micrograms/ml for 6 hours, CHML is able to suppress 50% of the tumor cell colony formation. At a concentration of 100 micrograms/ml (the therapeutic dosage in the clinical trial), more than 90% of the cells were killed in human breast carcinoma MCF-7, colorectal carcinoma RKO, kidney carcinoma G410, lung carcinoma and human myeloid leukemia ML-1 lines. In contrast, growth suppression of non-cancerous human skin fibroblasts by CHML was observed much less than that seen in tumor lines. These results indicate that CHML is an efficient inhibiting agent in tumor cell growth and is able to generate greater suppression in tumor cells than in noncancerous cells. With the use of DNA fragmentation assay, CHML was found to induce apoptosis in MCF-7, ML-1, H1299 and RKO lines after treatment at a concentration of 75 micrograms/ml for 8 hours. Following the CHML treatment, the tumor suppressor p53 protein elevated in RKO cells at 2 h posttreatment. The induction of p53 reached a peak at 4 hr and returned to normal level 16 hr later. Consistent with this result, Bax, which is regulated by p53 and is able to promote apoptosis, was also found to increase in a same kinetic manner as p53. These results suggest that the p53-pathway is activated by CHML and the activation of p53 may contribute to CHML-induced apoptosis in some tumor cells, such as MCF-7, RKO and ML-1. Considering that CHML is able to induce apoptosis in H1299 cells, which are of p53-negative status, it is speculated that CHML induces programmed cell death through both the p53-dependent and- independent pathways.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Zhan Q,Xu Zsubject
Has Abstractpub_date
1999-07-01 00:00:00pages
2893-9issue
4Beissn
0250-7005issn
1791-7530journal_volume
19pub_type
杂志文章abstract:BACKGROUND/AIM:The main objective of the present study was to evaluate the significance of apoptosis in the Fletcher's risk classification for gastrointestinal stromal tumors (GISTs). MATERIALS AND METHODS:Apoptotic cells were identified by immunostaining for single-stranded DNA (ssDNA). We assigned each GIST to one o...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.12542
更新日期:2018-05-01 00:00:00
abstract:BACKGROUND:The proportion of geriatric gastric cancer (GC) patients with comorbidities has been increasing in Japan. This study was designed to evaluate the prognostic influence of the extent of lymph node dissection (END) and perioperative morbidities in patients with GC. PATIENTS AND METHODS:Between 2008 and 2013, t...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2016-04-01 00:00:00
abstract:BACKGROUND:Peroxisome proliferator activated receptor gamma (PPARgamma) involvement in thyroid tumorigenesis has recently been studied, especially in follicular neoplasms. Conflicting results concerning the regulation of this receptor in human papillary carcinoma have been reported. Therefore, we quantitatively assesse...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2004-05-01 00:00:00
abstract:BACKGROUND:The safety and advantages of perioperative autologous blood transfusion (ABT) were evaluated on hepatectomy for hepatocellular carcinoma (HCC). MATERIALS AND METHODS:Blood samples were obtained and stored from 30 patients with HCC. HCC cells were investigated by the presence of AFPmRNA using RT-PCR after st...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2001-09-01 00:00:00
abstract:BACKGROUND:The aim of the study was to document the delay in diagnosis of Hodgkin's disease (HD) and clarify the impact on clinical stage and survival in our region. MATERIAL AND METHODS:A retrospective study of the records of 50 patients treated for primary HD in northern-Norway in the time period 1985 - 1993 was per...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1995-11-01 00:00:00
abstract::Since the discovery of cisplatin about 40 years ago, the design of innovative metal-based anticancer drugs is a growing area of research. Transition metal coordination complexes offer potential advantages over the more common organic-based drugs, including a wide range of coordination number and geometries, accessible...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:2014-01-01 00:00:00
abstract::Human peripheral blood monocytes derived from normal donors, melanoma patients (MP) before and after chemotherapy (MPa) were assayed for their capacity to inhibit SK-MEL-28 melanoma cell growth in vitro; in addition, growth modulating effects by prothymosin alpha 1 (ProTa) were studied. After preincubation with or wit...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1994-11-01 00:00:00
abstract:PURPOSE:To further evaluate lung cancer risk associated with rare Hras1 VNTR alleles and possible biological mechanisms. MATERIALS AND METHODS:The Hras1 VNTR was genotyped in 295 lung cancer patients and 500 healthy controls by PCR and high resolution electrophoresis. Microsatellite alterations were examined in 168 tu...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1999-11-01 00:00:00
abstract::Ascorbate has dose-dependent inverse effects on cancer cells growth and expression of matrixmetalloproteinases (MMP) and transforming growth factor-beta (TGF-beta), which regulate extracellular matrix (ECM) remodeling. We examined melanoma cell viability and ECM remodeling mechanisms of ascorbate and its modulation by...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2009-08-01 00:00:00
abstract:UNLABELLED:Distant metastases to the oral cavity, the face or facial skeleton are rare and/in are usually found in such locations at a late stage of the malignancy. In the majority of cases, the malignancy is already known at the time of admission. However, a distant metastasis as the first sign of a cancer developing ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2010-05-01 00:00:00
abstract::T-CAR1 is a human carcinoma cell line established from a brain metastasis. The tumour cells overexpress EGFR and contain an amplified EGFR gene. In vitro in the presence of 5% human serum the tumour cells grow as adherent cells in monolayer. Shortly after exposure to EGF a large number of tumour cells round up and det...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1995-05-01 00:00:00
abstract::The small molecular weight protein (p7) was overexpressed in the human ovarian carcinoma cell lines, SKVLB600 (selected for vinblastine resistance from SKOV3 cells). OVCAR 4/ADR100 (selected for doxorubicin resistance from NIH:OVCAR4) and (OVCAR4/VBL200 (selected for vinblastine resistance from NIH:OVCAR4). Trace amou...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::Tumor-specific antigens are important in the study of tumor biology, tumor diagnosis and prognosis and possibly in tumor therapy. We demonstrate in this report that surgical specimens of colon, breast and ovarian tumors in native-state in vitro gel-supported three-dimensional in vitro histoculture maintain in vivo-lik...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1991-03-01 00:00:00
abstract::TPS was determined by immunohistochemistry in 44 different tissues (normal and pathological) from various organs such as cervix, endometrium, breast, stomach, bowel and bladder. TPS was expressed in several physiologic situations: in different phases of the endometrial cycle and in cervical metaplasia. In other organs...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1994-03-01 00:00:00
abstract::The poor response of pancreatic adenocarcinoma to conventional chemotherapy has prompted us to search for innovative treatment approaches. Verapamil, a calcium channel blocker, has been reported to increase the doxorubicin sensitivity of several tumor models with acquired resistance, often in association with decrease...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1989-03-01 00:00:00
abstract:BACKGROUND/AIM:Cold atmospheric plasma (CAP) attenuates tumor cell proliferation and induces apoptosis in various cell lines. While exerting marginal effects on non-neoplastic cells this unfolds promising applications in cancer therapy. The aim of the study was to analyse the effects of different CAP sources and applic...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.11968
更新日期:2017-10-01 00:00:00
abstract:BACKGROUND:PS2-expression is controlled by estrogens and is a prognostic factor for long-term and post-relapse survival. Breast carcinomas associated with a BRCA1 mutation (BRCA1-BC'S) exhibit many specific features, particularly for ER expression. Our purpose was to determine if pS2-expression was different in BRCA1-B...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2001-07-01 00:00:00
abstract:BACKGROUND/AIM:The role of cathepsin-D is well established in breast cancer progression, being correlated with worse clinical outcomes. However, to our knowledge, no study has been performed investigating its expression in primary breast cancer tumors and their corresponding recurrences or metastasis. MATERIALS AND ME...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2012-03-01 00:00:00
abstract::Recently, it has been reported that total clearance (CLtot) of vancomycin is significantly higher in patients with malignancies compared to those without malignancies. In the present study, to clarify the mechanism of this enhancement in malignancy, we adopted rat animal models, using chemical carcinogen-induced osteo...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2012-03-01 00:00:00
abstract:BACKGROUND:The benefit of tissue microarray (TMA) is its capability to analyse large numbers of tissue samples in a uniform fashion. This study was designed to evaluate the capability of TMA for analyzing the status of death-associated protein kinase 1 (DAPK1) in breast cancer patient and to explore its potential in th...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2011-10-01 00:00:00
abstract:BACKGROUND:Embryonic stem cell expressed Ras (ERas) oncogene is associated with the tumorigenicity of embryonic stem cells. The aim of this study was to clarify the significance of ERas expression in clinical samples of gastric carcinomas. MATERIALS AND METHODS:Three hundred and few tissues from gastric cancer patient...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2009-06-01 00:00:00
abstract:BACKGROUND:Histone acetyltransferase E1A-binding protein p300 (EP300), tumor protein p53 (TP53) and B-cell lymphoma-2-associated X protein (BAX) contribute to the regulation of the cell cycle and apoptosis, cellular processes that are often impaired in cancer cells. The aim of this study was to determine the expression...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.11646
更新日期:2017-06-01 00:00:00
abstract:AIM:To evaluate whether the congested area that develops in associating liver partition with portal vein ligation for staged hepatectomy (ALPPS) contributes to rapid future liver remnant (FLR) hypertrophy. PATIENTS AND METHODS:Eight patients undergoing liver partition and right portal vein (RPV) ligation within the FL...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.11028
更新日期:2016-09-01 00:00:00
abstract:BACKGROUND:The optimal palliative chemotherapy in endometrial cancer is unknown. CASE REPORT:A patient was diagnosed to have primary endometrioid endometrial cancer metastatic to the pelvic, paraaortic and right inguinal lymph nodes (FIGO IVb). After radical surgery, she received 6 adjuvant cycles of cisplatin and epi...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2009-05-01 00:00:00
abstract:BACKGROUND:Complementary alternative medicine (CAM) is widely used among patients with cancer. This usage may have potentially harmful effects, especially when combined with anticancer drugs. However, some complementary methods may benefit patients. This review investigated the prevalence of CAM use among patients with...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:2016-07-01 00:00:00
abstract:BACKGROUND:The effects of oxaliplatin (L-OHP) on cell cycle and apoptosis were examined. MATERIALS AND METHODS:The HT29, MCF7, Hela and A549 cell lines were treated and dual parameter flow cytometry BrdU/PI was then performed to monitor cell cycle modifications. Annexin V-FITC and PI probes were used for the detection...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2006-05-01 00:00:00
abstract::One hundred and eighty-seven patients with a transitional cell bladder cancer were followed by retrospectively for a mean of 9.5 years. Clinical stage (p less than 0.0001), histological grade (p less than 0.0001), papillary (p less than 0.0001), SPF (p less than 0.0001), M/V index (p less than 0.0001), MAI (p less tha...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1991-03-01 00:00:00
abstract:BACKGROUND:Early detection before scintigraphic appearance of osseous metastatic spread might improve the outcome of breast cancer patients. The amino-terminal propeptide (PINP) of type I collagen as an indicator of bone formation is a very promising candidate among all markers of bone metabolism. We investigated the u...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2005-05-01 00:00:00
abstract:BACKGROUND:Increasing evidence suggests that a pro-inflammatory microenvironment affects distant metastasis of breast cancer cells, in particular by favoring tumor cell adhesion to endothelium. The aim of this study was to investigate the potential of different anti-inflammatory drugs to inhibit this effect in vitro. ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2012-03-01 00:00:00
abstract::The effect of 4,6-benzylidene-D-glucose (BG) on chemically induced primary hepatocellular carcinomas in rats was tested after daily single injections of 85 mg per kg body weight into the aorta through a canula implanted through the right carotid artery. Treatment started immediately following a biopsy confirming the d...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1986-03-01 00:00:00