Abstract:
:The technique of comparative genomic hybridisation (CGH) has until recently been used to screen for common genomic abnormalities in fresh tumour material; it has identified previously unrecognised regions of amplification associated with poor prognosis subtypes of breast cancer and lymphoma. Our group has applied this technique to resistant cell lines and their sensitive counterparts in order to define chromosomal abnormalities associated with acquired drug resistance. We have demonstrated the applicability of this technique to the study of drug resistance using cell lines with known mechanisms of resistance. The ability to detect novel genomic alterations in cell lines with novel mechanisms of resistance was also demonstrated. We subsequently examined the CGH profiles of seven different cell lines made resistant to three platinum analogues and showed the most consistent abnormalities to involve over-representation of regions 4q and 6q. More recently, we have applied the CGH technique to a series of testicular germ cell tumours (TGCTs) collected as formalin-fixed paraffin-embedded biopsy specimens from patients, both pre- and post-therapy using a platinum-based regimen (POMB/ACE). Previous reports have shown over-representation of X, 7q, 8q and 12p and loss of 13q to occur in 25% of primary TGCTs. Over-representation of 12p was confirmed in the majority of these biopsy samples; deletion of 13q was noted in the initial biopsies of several patients. We also demonstrated alterations of 4p, 4q, 5q and 6q in this series of patients. Newly acquired deletions of 2q and 18q and amplifications of 8q were frequently observed in post-chemotherapy samples from resistant tumours. The CGH studies on these patients with TGCT will not only enable us to correlate our observations on clinical material with those from long-term cell lines, but should also identify sites of key genes involved in clinical platinum resistance.
journal_name
J Inorg Biochemjournal_title
Journal of inorganic biochemistryauthors
Hiorns LR,Seckl MJ,Paradinas F,Sharp SY,Skelton LA,Brunstrom G,Newlands ES,Kelland LR,Leyland-Jones Bdoi
10.1016/s0162-0134(99)00140-3subject
Has Abstractpub_date
1999-10-01 00:00:00pages
95-104issue
1-2eissn
0162-0134issn
1873-3344pii
S0162-0134(99)00140-3journal_volume
77pub_type
杂志文章abstract::The plasmid DNA scission by the restriction enzyme HaeIII was investigated in the presence of tetrakis(1-methylpyridinium-4-yl)porphyrin (H2TMPyP) and its manganese(III), iron(III), nickel(II), cobalt(III) and zinc(II) derivatives. The effect of metalloporphyrins on plasmid DNA cleavage was ascertained by gel electrop...
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journal_title:Journal of inorganic biochemistry
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journal_title:Journal of inorganic biochemistry
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abstract::Cancer-Associated Fibroblasts (CAFs) contribute to tumour progression and have received significant attention as a therapeutic target. These cells produce growth factors, cytokines and chemokines, stimulating cancer cell proliferation and inhibiting their apoptosis. Recent advances in drug delivery have demonstrated a...
journal_title:Journal of inorganic biochemistry
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journal_title:Journal of inorganic biochemistry
pub_type: 杂志文章
doi:10.1016/j.jinorgbio.2009.05.019
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journal_title:Journal of inorganic biochemistry
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journal_title:Journal of inorganic biochemistry
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doi:10.1016/0162-0134(91)85006-3
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journal_title:Journal of inorganic biochemistry
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更新日期:2016-10-01 00:00:00
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doi:10.1016/j.jinorgbio.2007.04.016
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journal_title:Journal of inorganic biochemistry
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doi:10.1016/j.jinorgbio.2014.06.014
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journal_title:Journal of inorganic biochemistry
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journal_title:Journal of inorganic biochemistry
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journal_title:Journal of inorganic biochemistry
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journal_title:Journal of inorganic biochemistry
pub_type: 杂志文章
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更新日期:2014-07-01 00:00:00
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journal_title:Journal of inorganic biochemistry
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journal_title:Journal of inorganic biochemistry
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journal_title:Journal of inorganic biochemistry
pub_type: 杂志文章
doi:10.1016/0162-0134(91)84004-s
更新日期:1991-05-15 00:00:00
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journal_title:Journal of inorganic biochemistry
pub_type: 杂志文章
doi:10.1016/j.jinorgbio.2015.08.014
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journal_title:Journal of inorganic biochemistry
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journal_title:Journal of inorganic biochemistry
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更新日期:2005-11-01 00:00:00
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journal_title:Journal of inorganic biochemistry
pub_type: 杂志文章
doi:10.1016/s0162-0134(00)00235-x
更新日期:2001-04-01 00:00:00
abstract::Composites of beta-chitin with calcium carbonate polymorphs were prepared by precipitation of the mineral into a chitin scaffold by means of a double diffusion system. The beta-chitin was obtained from the pen of the Loligo sp. squid. The three main polymorphs of calcium carbonate: aragonite, calcite and vaterite, wer...
journal_title:Journal of inorganic biochemistry
pub_type: 杂志文章
doi:10.1016/s0162-0134(02)00471-3
更新日期:2002-08-30 00:00:00
abstract::The action mechanism of vanadocene dichloride, Cp2VCl2 (Cp=eta5-C5H5), has been investigated by interaction with human serum transferrin for its promising antitumor activities. Our results have shown that Cp2VCl2 binds to transferrin and form a new complex, and the calculated apparent association constant is 1.37 x 10...
journal_title:Journal of inorganic biochemistry
pub_type: 杂志文章
doi:10.1016/j.jinorgbio.2007.07.030
更新日期:2008-01-01 00:00:00