Abstract:
:A neuropeptide F (NPF) was isolated from the fruit fly, Drosophila mellanogaster, based on a radioimmunoassay for a gut peptide from the corn earworm, Helicoverpa zea. A partial sequence was obtained from the fly peptide, and a genomic sequence coding for NPF was cloned after inverse polymerase chain reaction and shown to exist as a single genomic copy. The encoded, putative prepropeptide can be processed into an amidated NPF with 36 residues that is related to invertebrate NPF's and the neuropeptide Y family of vertebrates. In situ hybridization and immunocytochemistry showed that Drosophila NPF was expressed in the brain and midgut of fly larvae and adults.
journal_name
Peptidesjournal_title
Peptidesauthors
Brown MR,Crim JW,Arata RC,Cai HN,Chun C,Shen Pdoi
10.1016/s0196-9781(99)00097-2subject
Has Abstractpub_date
1999-01-01 00:00:00pages
1035-42issue
9eissn
0196-9781issn
1873-5169pii
S0196-9781(99)00097-2journal_volume
20pub_type
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