Coagulase gene polymorphism of Staphylococcus aureus isolates from dairy cattle in different geographical areas.

Abstract:

:The objectives of this study were to investigate the coagulase gene polymorphism of Staphylococcus aureus isolates obtained from bovine mastitic milk and to determine the resistance of predominant and rare coagulase genotypes to bovine blood neutrophil bactericidal activities. A total of 453 isolates were collected from four countries: the Czech Republic, France, Korea and the United States. The isolates were subtyped into 40 types by restriction fragment length polymorphism (RFLP) of the coagulase gene. Twenty-three strains from predominant and rare genotypes were evaluated for their ability to resist neutrophil bactericidal activities. There were significant (P < 0.01) differences in the average percent neutrophil killing of the predominant (16.7%) and rare (39.7%) genotypes when bacteria were opsonized with antiserum. The results indicate that the profiles of coagulase genotype differ among geographic locations, and only a few genotypes prevail in each location. In addition, the predominant genotypes were more resistant to neutrophil bactericidal activities than rare genotypes.

journal_name

Epidemiol Infect

authors

Su C,Herbelin C,Frieze N,Skardova O,Sordillo LM

doi

10.1017/s0950268899002228

subject

Has Abstract

pub_date

1999-04-01 00:00:00

pages

329-36

issue

2

eissn

0950-2688

issn

1469-4409

journal_volume

122

pub_type

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