Ba2+ does not support synaptic vesicle retrieval in rat cerebrocortical synaptosomes.

Abstract:

:To investigate whether any specific requirement for extracellular Ca2+ exists in the control synaptic vesicle retrieval, we examined the ability of the divalent cation Ba2+ to substitute for Ca2+ in both vesicle exocytosis and endocytosis. Ba2+ stimulated glutamate release from rat cerebrocortical synaptosomes. Ba2+-evoked release was inhibited by bafilomycin A1, indicating release was via exocytosis of synaptic vesicles. However, Ba2+ did not stimulate vesicle retrieval, monitored by a FM2-10-based retrieval assay. Therefore synaptic vesicle retrieval in central nerve terminals has a specific requirement for extracellular Ca2+ and the Ca2+ receptor for retrieval has a different cation specificity to the Ca2+ receptor for exocytosis.

journal_name

Neurosci Lett

journal_title

Neuroscience letters

authors

Cousin MA,Robinson PJ

doi

10.1016/s0304-3940(98)00610-7

subject

Has Abstract

pub_date

1998-08-28 00:00:00

pages

1-4

issue

1

eissn

0304-3940

issn

1872-7972

pii

S0304-3940(98)00610-7

journal_volume

253

pub_type

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