The 2.8-A structure of rat liver F1-ATPase: configuration of a critical intermediate in ATP synthesis/hydrolysis.

abstract：:Host factor protein Cyclophilin A (CypA) regulates HIV-1 viral infectivity through direct interactions with the viral capsid, by an unknown mechanism. CypA can either promote or inhibit viral infection, depending on host cell type and HIV-1 capsid (CA) protein sequence. We have examined the role of conformational dyna...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lu M,Hou G,Zhang H,Suiter CL,Ahn J,Byeon IJ,Perilla JR,Langmead CJ,Hung I,Gor'kov PL,Gan Z,Brey W,Aiken C,Zhang P,Schulten K,Gronenborn AM,Polenova T

更新日期：2015-11-24 00:00:00

abstract：:Translocator protein (TSPO) is a key member of the mitochondrial cholesterol transport complex in steroidogenic tissues. To assess the function of TSPO, we generated two lines of Cre-mediated Tspo conditional knockout (cKO) mice. First, gonadal somatic cell-targeting Amhr2-Cre mice were crossed with Tspo-floxed mice t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fan J,Campioli E,Midzak A,Culty M,Papadopoulos V

更新日期：2015-06-09 00:00:00

abstract：:Previous studies have shown that binding of interleukin 1 (IL-1) to its receptor and intracellular processing of the IL-1/IL-1 receptor complex appear to be different in B- and T-lymphocyte cell lines. In this study we used a B-lymphoid cell line, 70Z/3, and T-lymphoid cell line, EL-4 6.1 C10, to explore further the d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bomsztyk K,Sims JE,Stanton TH,Slack J,McMahan CJ,Valentine MA,Dower SK

更新日期：1989-10-01 00:00:00

abstract：:Forkhead box g1 (Foxg1) is a nuclear-cytosolic transcription factor essential for the forebrain development and involved in neurodevelopmental and cancer pathologies. Despite the importance of this protein, little is known about the modalities by which it exerts such a large number of cellular functions. Here we show ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pancrazi L,Di Benedetto G,Colombaioni L,Della Sala G,Testa G,Olimpico F,Reyes A,Zeviani M,Pozzan T,Costa M

更新日期：2015-11-10 00:00:00

abstract：:Astronomers and physicists noticed centuries ago that visual spatial resolution is higher for dark than light stimuli, but the neuronal mechanisms for this perceptual asymmetry remain unknown. Here we demonstrate that the asymmetry is caused by a neuronal nonlinearity in the early visual pathway. We show that neurons ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kremkow J,Jin J,Komban SJ,Wang Y,Lashgari R,Li X,Jansen M,Zaidi Q,Alonso JM

更新日期：2014-02-25 00:00:00

abstract：:A series of monoclonal antibodies was used to define three discrete stages of human intrathymic T-cell differentiation. The earliest stage was confined to <10% of thymocytes, which were.reactive with both OKT9 and OKT10. Subsequently, approximately 70% of human thymocytes acquired a thymocyte-restricted antigen, OKT6,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reinherz EL,Kung PC,Goldstein G,Levey RH,Schlossman SF

更新日期：1980-03-01 00:00:00

abstract：:In early brain development, ascending thalamocortical axons (TCAs) navigate through the ventral telencephalon (VTel) to reach their target regions in the young cerebral cortex. Descending, deep-layer cortical axons subsequently target appropriate thalamic and subcortical target regions. However, precisely how and when...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Robichaux MA,Chenaux G,Ho HY,Soskis MJ,Dravis C,Kwan KY,Šestan N,Greenberg ME,Henkemeyer M,Cowan CW

更新日期：2014-02-11 00:00:00

abstract：:Nucleosomes in eukaryotes act as platforms for the dynamic integration of epigenetic information. Posttranslational modifications are reversibly added or removed and core histones exchanged for paralogous variants, in concert with changing demands on transcription and genome accessibility. Histones are also common in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stevens KM,Swadling JB,Hocher A,Bang C,Gribaldo S,Schmitz RA,Warnecke T

更新日期：2020-12-29 00:00:00

abstract：:The lack of efficient mechanisms for stable genetic transformation of medically important insects, such as anopheline mosquitoes, is the single most important impediment to progress in identifying novel control strategies. Currently available techniques for foreign gene expression in insect cells in culture lack the b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Matsubara T,Beeman RW,Shike H,Besansky NJ,Mukabayire O,Higgs S,James AA,Burns JC

更新日期：1996-06-11 00:00:00

abstract：:The formation of amyloid-like aggregates by expanded polyglutamine (polyGln) sequences is suspected to play a critical role in the neuropathology of Huntington's disease and other expanded CAG-repeat diseases. To probe the folding of the polyGln sequence in the aggregate, we replaced Gln-Gln pairs at different sequenc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thakur AK,Wetzel R

更新日期：2002-12-24 00:00:00

abstract：:A flexible finite difference method is described that gives approximate solutions of linear elliptic partial differential equations, Lu = G, subject to general linear boundary conditions. The method gives high-order accuracy. The values of the unknown approximation function U are determined at mesh points by solving a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lynch RE,Rice JR

更新日期：1978-06-01 00:00:00

abstract：:C1q-like genes (C1ql1-C1ql4) encode small, secreted proteins that are expressed in differential patterns in the brain but whose receptors and functions remain unknown. BAI3 protein, in contrast, is a member of the cell-adhesion class of G protein-coupled receptors that are expressed at high levels in the brain but who...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bolliger MF,Martinelli DC,Südhof TC

更新日期：2011-02-08 00:00:00

abstract：:Assemblies formed by solid particles at interfaces have been widely studied because they serve as models of molecular phenomena, including molecular self-assembly. Solid particles adsorbed at interfaces also provide a means of stabilizing liquid-liquid emulsions and synthesizing materials with tunable mechanical, opti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Koenig GM Jr,Lin IH,Abbott NL

更新日期：2010-03-02 00:00:00

abstract：:Spatial working memory or short-term place memory is impaired in schizophrenia. The efficiency of antipsychotic drugs, particularly of typical antipsychotics, on cognitive deficit in schizophrenia remains disputable. Inhibition of serotonin (5-HT) 2A/2C receptors is important for cognitive improvement in schizophrenic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bubenikova-Valesova V,Stuchlik A,Svoboda J,Bures J,Vales K

更新日期：2008-01-22 00:00:00

abstract：:Twice each year, millions of night-migratory songbirds migrate thousands of kilometers. To find their way, they must process and integrate spatiotemporal information from a variety of cues including the Earth's magnetic field and the night-time starry sky. By using sensory-driven gene expression, we discovered that ni...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mouritsen H,Feenders G,Liedvogel M,Wada K,Jarvis ED

更新日期：2005-06-07 00:00:00

abstract：:To examine the regulation of midbrain dopamine neurons, recordings were obtained from single neurons of freely moving, genetically engineered dopamine-deficient (DD) mice. DD mice were tested without dopamine signaling (basal state) and with endogenous dopamine signaling (after L-dopa administration). In the basal sta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Robinson S,Smith DM,Mizumori SJ,Palmiter RD

更新日期：2004-09-07 00:00:00

abstract：:The structures of the major folding intermediate, the transition state for folding, and the folded state of barnase have been previously characterized. We now add a further step toward a complete picture of the folding of barnase by reporting the backbone 15N, 13C, and 1H NMR assignments for barnase unfolded at pH 1.8...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Arcus VL,Vuilleumier S,Freund SM,Bycroft M,Fersht AR

更新日期：1994-09-27 00:00:00

abstract：:In many plant species only a small proportion of buds yield branches. Both the timing and extent of bud activation are tightly regulated to produce specific branching architectures. For example, the primary shoot apex can inhibit the activation of lateral buds. This process is termed apical dominance and is dependent ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Prusinkiewicz P,Crawford S,Smith RS,Ljung K,Bennett T,Ongaro V,Leyser O

更新日期：2009-10-13 00:00:00

abstract：:Climate change is expected to influence the capacities of the land and oceans to act as repositories for anthropogenic CO2 and hence provide a feedback to climate change. A series of experiments with the National Center for Atmospheric Research-Climate System Model 1 coupled carbon-climate model shows that carbon sink...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fung IY,Doney SC,Lindsay K,John J

更新日期：2005-08-09 00:00:00

abstract：:Flowering time (FT) is the developmental transition coupling an internal genetic program with external local and seasonal climate cues. The genetic loci sensitive to predictable environmental signals underlie local adaptation. We dissected natural variation in FT across a new global diversity set of 473 unique accessi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li Y,Huang Y,Bergelson J,Nordborg M,Borevitz JO

更新日期：2010-12-07 00:00:00

abstract：:A human peripheral T-cell cDNA library was screened with two labeled synthetic oligonucleotides encoding regions of a human placenta protein-tyrosine-phosphatase (protein-tyrosine-phosphate phosphohydrolase, EC 3.1.3.48). One positive clone was isolated and the nucleotide sequence was determined. It contained 1305 bas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cool DE,Tonks NK,Charbonneau H,Walsh KA,Fischer EH,Krebs EG

更新日期：1989-07-01 00:00:00

abstract：:Amyloids are often associated with pathologic processes such as in Alzheimer's disease (AD), but can also underlie physiological processes such as pigmentation. Formation of pathological and functional amyloidogenic substrates can require precursor processing by proteases, as exemplified by the generation of Aβ peptid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rochin L,Hurbain I,Serneels L,Fort C,Watt B,Leblanc P,Marks MS,De Strooper B,Raposo G,van Niel G

更新日期：2013-06-25 00:00:00

abstract：:Programmed death-1 (PD-1) regulates T cell exhaustion during chronic infections. Blocking the PD-1:PD-ligand (PD-L) pathway reinvigorates exhausted CD8 T cells. Exactly how blocking PD-1:PD-L interactions improves T cell immunity, however, remains unclear. PD-1:PD-L blockade could reprogram all exhausted T cells to be...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Blackburn SD,Shin H,Freeman GJ,Wherry EJ

更新日期：2008-09-30 00:00:00

abstract：:The temporal dynamics of electrical activity in an olfactory organ, the procerebral lobe of the terrestrial mollusc Limax maximus, is studied. The lobe exhibits intrinsic oscillations in its field potential. Intracellular recordings show that the lobe contains two classes of neurons, both with activity phase-locked to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Delaney KR,Gelperin A,Fee MS,Flores JA,Gervais R,Tank DW,Kleinfeld D

更新日期：1994-01-18 00:00:00

abstract：:Because of increased numbers of recorded pertussis cases in the United States, this study sought to understand the role of nonmedical vaccine exemptions and waning immunity may have had on the resurgence of pertussis in the United States at the community level. We used geospatial scan statistics, SaTScan, version 9.4,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aloe C,Kulldorff M,Bloom BR

更新日期：2017-07-03 00:00:00

abstract：:Endothelial cell growth factor (ECGF) binds specifically in vitro to membrane receptors present on the surface of several cell types, including murine and human endothelial cells and fibroblasts. Monoclonal antibodies prepared against ECGF that inhibit the mitogenic activity of the growth factor prevent receptor occup...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schreiber AB,Kenney J,Kowalski WJ,Friesel R,Mehlman T,Maciag T

更新日期：1985-09-01 00:00:00

abstract：:Escherichia coli glutamine synthetase (GS) was inactivated by a nonenzymic mixed-function oxidation system composed of ascorbate, O2, and Fe(III). Partial inactivation of GS by this system leads to the formation of hybrid GS molecules (dodecamers) composed of both active and inactive subunits. Subunit interactions in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakamura K,Stadtman ER

更新日期：1984-04-01 00:00:00

abstract：:Antiviral and macrophage-priming activities in the supernatant medium of a subclone of a concanavalin A-stimulated mouse T-cell hybridoma were investigated. The two activities were associated with a molecular weight of approximately 50,000 and could not be separated by various approaches. Both activities were eliminat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pace JL,Russell SW,Schreiber RD,Altman A,Katz DH

更新日期：1983-06-01 00:00:00

abstract：:The regulation of initiation of DNA replication is crucial to ensure that the genome is replicated only once per cell cycle. In the Gram-positive bacterium Bacillus subtilis, the function of the YabA protein in initiation control was assigned based on its interaction with the DnaA initiator and the DnaN sliding clamp ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Noirot-Gros MF,Velten M,Yoshimura M,McGovern S,Morimoto T,Ehrlich SD,Ogasawara N,Polard P,Noirot P

更新日期：2006-02-14 00:00:00

abstract：:Whole-cell DNA preparations isolated from SC-1 cells chronically infected with N- or B-tropic murine leukemia viruses (MuLV) were tested for infectious activity in an Fv-1n (NIH-3T3) and two Fv-Ib (C57BL/6 and SV-A31) cell cultures. Efficiency of transfection for all DNAs was better in the NIH-3T3 cells than in C57BL/...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hsu IC,Yang WK,Tennant RW,Brown A

更新日期：1978-03-01 00:00:00