Inhibition of translation and bacterial growth by peptide nucleic acid targeted to ribosomal RNA.

abstract：:A (Mg(2+) + Ca(2+))ATPase (ATP phosphohydrolase, EC 3.6.1.3) has been purified from sarcoplasmic reticulum using a single step centrifugation procedure. The preparation is >95% pure by weight and contains only 25-30% of the lipid associated with the enzyme in native sarcoplasmic reticulum. The purified enzyme is unabl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Warren GB,Toon PA,Birdsall NJ,Lee AG,Metcalfe JC

更新日期：1974-03-01 00:00:00

abstract：:Protein-binding DNA sequence elements encode a variety of regulated functions of genomes. Information about such elements is currently in a state of rapid growth, but improved methods are required to characterize the sequence specificity of DNA-binding proteins. We have established an in vitro method for specific and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gustafsdottir SM,Schlingemann J,Rada-Iglesias A,Schallmeiner E,Kamali-Moghaddam M,Wadelius C,Landegren U

更新日期：2007-02-27 00:00:00

abstract：:We tested the possibility that insulin-like growth factor I (IGF-I) acts as a neuromodulator in the adult cerebellar cortex since previous observations indicated that IGF-I is located in the olivo-cerebellar system encompassing the inferior olive and Purkinje cells. We found that conjoint administration of IGF-I and g...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Castro-Alamancos MA,Torres-Aleman I

更新日期：1993-08-01 00:00:00

abstract：:The HIV envelope (Env) protein mediates entry into cells by binding CD4 and an appropriate coreceptor, which triggers structural changes in Env that lead to fusion between the viral and cellular membranes. The major HIV-1 coreceptors are the seven transmembrane domain chemokine receptors CCR5 and CXCR4. The type of co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoffman TL,Canziani G,Jia L,Rucker J,Doms RW

更新日期：2000-10-10 00:00:00

abstract：:Five protease inhibitors with different modes of action were found to reduce the growth of transformed mouse (Py3T3, SV3T3, and 3T12) and hamster (PyBHK) cells. Some of these inhibitors caused the transformed cells to cease growth at saturation densities characteristic for nontransformed cells. The protease inhibitors...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schnebli HP,Burger MM

更新日期：1972-12-01 00:00:00

abstract：:Thioredoxins are small multifunctional redox active proteins widely if not universally distributed among living organisms. In chloroplasts, two types of thioredoxins (f and m) coexist and play central roles in regulating enzyme activity. Reduction of thioredoxins in chloroplasts is catalyzed by an iron-sulfur disulfid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Balmer Y,Koller A,del Val G,Manieri W,Schürmann P,Buchanan BB

更新日期：2003-01-07 00:00:00

abstract：:Cytochrome P450 enzymes are monooxygenases that contain a functional heme b group linked to a conserved cysteine with a thiolate bond. In the native state, the central iron atom is hexacoordinated with a covalently bound water molecule. The exclusion of solvent molecules from the active site is essential for efficient...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oprea TI,Hummer G,Garcia AE

更新日期：1997-03-18 00:00:00

abstract：:We report here the discovery of a small molecule inhibitor of pestivirus replication. The compound, designated VP32947, inhibits the replication of bovine viral diarrhea virus (BVDV) in cell culture at a 50% inhibitory concentration of approximately 20 nM. VP32947 inhibits both cytopathic and noncytopathic pestiviruse...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baginski SG,Pevear DC,Seipel M,Sun SC,Benetatos CA,Chunduru SK,Rice CM,Collett MS

更新日期：2000-07-05 00:00:00

abstract：:The cytokine IFN-γ is a critical regulator of immune system development and function. Almost all leukocytes express the receptor for IFN-γ, yet each cell type elicits a different response to this cytokine. Cell type-specific effects of IFN-γ make it difficult to predict the outcomes of the systemic IFN-γ blockade and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Krummel MF,Mahale JN,Uhl LFK,Hardison EA,Mujal AM,Mazet JM,Weber RJ,Gartner ZJ,Gérard A

更新日期：2018-11-06 00:00:00

abstract：:Mitogen-stimulated scheduled DNA synthesis and DNA excision repair in human lymphocytes, as well as DNA polymerase a activity in a cell-free system, were inhibited by an electrophilic metabolite of benzo[a]pyrene. This metabolite, (+/-)-anti-(7r,8t)-dihydroxy-(9,10t)-epoxy-7,8,9,10-tetrahyd robenzo[a]pyrene (BPDE), co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Busbee DL,Joe CO,Norman JO,Rankin PW

更新日期：1984-09-01 00:00:00

abstract：:CDK7 is a cyclin-dependent kinase proposed to function in two essential cellular processes: transcription and cell cycle regulation. CDK7 is the kinase subunit of the general transcription factor TFIIH that phosphorylates the C-terminal domain (CTD) of RNA polymerase II, and has been shown to be broadly required for t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wallenfang MR,Seydoux G

更新日期：2002-04-16 00:00:00

abstract：:Calmodulin (CaM) is a second messenger protein that has evolved to bind tightly to a variety of targets and, as such, exhibits low binding specificity. We redesigned CaM by using a computational protein design algorithm to improve its binding specificity for one of its targets, smooth muscle myosin light chain kinase ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shifman JM,Mayo SL

更新日期：2003-11-11 00:00:00

abstract：:The photoaffinity labeling agent 5-azidoindole-3-acetic acid, an analog of the endogenous plant hormone indole-3-acetic acid (an auxin), was used to identify indole-3-acetic acid-binding proteins in maize. Two peptides with subunit molecular masses of 24 and 22 kilodaltons are specifically labeled in a saturable manne...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jones AM,Venis MA

更新日期：1989-08-01 00:00:00

abstract：:The synthesis of graphene materials is typically carried out by oxidizing graphite to graphite oxide followed by a reduction process. Numerous methods exist for both the oxidation and reduction steps, which causes unpredictable contamination from metallic impurities into the final material. These impurities are known ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wong CH,Sofer Z,Kubešová M,Kučera J,Matějková S,Pumera M

更新日期：2014-09-23 00:00:00

abstract：:The activities of cytosolic 3-hydroxy-3-methylglutaryl coenzyme A synthase [3-hydroxy-3-methylglutaryl-CoA acetoacetyl-CoA-lyase (CoA-acylating), EC 4.1.3.5] and microsomal 3-hydroxy-3-methylglutaryl coenzyme A reductase[mevalonate:NADP+ oxidoreductase (CoA-acylating), EC 1.1.1.34], two sequential enzymes in the chole...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Balasubramaniam S,Goldstein JL,Brown MS

更新日期：1977-04-01 00:00:00

abstract：:IL-17 is a T cell-derived, proinflammatory cytokine that is suspected to be involved in the development of various inflammatory diseases. Although there are elevated levels of IL-17 in synovial fluid of patients with rheumatoid arthritis, the pathogenic role of IL-17 in the development of rheumatoid arthritis remains ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakae S,Saijo S,Horai R,Sudo K,Mori S,Iwakura Y

更新日期：2003-05-13 00:00:00

abstract：:Previous studies have identified lipid-poor high density lipoproteins with electrophoretic pre-beta mobility as the initial acceptors of cell-derived cholesterol in human plasma. These lipoproteins contain apolipoprotein A-I (apo A-I) as their sole apolipoprotein. In the present study, incubation of human plasma with ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang Y,von Eckardstein A,Wu S,Maeda N,Assmann G

更新日期：1994-03-01 00:00:00

abstract：:In animal cells, exogenous DNA recombines into random chromosomal sites much more frequently than it recombines into homologous sites. Free DNA ends are "recombinogenic" in both processes. To test the effects of specific ends on analogous extrachromosomal processes, we constructed a linear genome of simian virus 40 wi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chang XB,Wilson JH

更新日期：1987-07-01 00:00:00

abstract：:Eye-opening represents a turning point in the function of the visual cortex. Before eye-opening, the visual cortex is largely devoid of sensory inputs and neuronal activities are generated intrinsically. After eye-opening, the cortex starts to integrate visual information. Here we used in vivo two-photon calcium imagi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rochefort NL,Garaschuk O,Milos RI,Narushima M,Marandi N,Pichler B,Kovalchuk Y,Konnerth A

更新日期：2009-09-01 00:00:00

abstract：:Genes carried by EcoRI endonuclease-generated fragments of Staphylococcus plasmid DNA have been covalently joined to the E. coli antibiotic-resistance plasmid pSC101, and the resulting hybrid molecules have been introduced into E. coli by transformation. The newly constructed plasmids replicate as biologically functio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chang AC,Cohen SN

更新日期：1974-04-01 00:00:00

abstract：:Lithium-sulfur batteries (Li-S batteries) have attracted intense interest because of their high specific capacity and low cost, although they are still hindered by severe capacity loss upon cycling caused by the soluble lithium polysulfide intermediates. Although many structure innovations at the material and device l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu W,Jiang J,Yang KR,Mi Y,Kumaravadivel P,Zhong Y,Fan Q,Weng Z,Wu Z,Cha JJ,Zhou H,Batista VS,Brudvig GW,Wang H

更新日期：2017-04-04 00:00:00

abstract：:Motifs of neural circuitry seem surprisingly conserved over different areas of neocortex or of paleocortex, while performing quite different sensory processing tasks. This apparent paradox may be resolved by the fact that seemingly different problems in sensory information processing are related by transformations (ch...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hopfield JJ

更新日期：1996-12-24 00:00:00

abstract：:The brain is assumed to be hypoactive during cardiac arrest. However, the neurophysiological state of the brain immediately following cardiac arrest has not been systematically investigated. In this study, we performed continuous electroencephalography in rats undergoing experimental cardiac arrest and analyzed change...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Borjigin J,Lee U,Liu T,Pal D,Huff S,Klarr D,Sloboda J,Hernandez J,Wang MM,Mashour GA

更新日期：2013-08-27 00:00:00

abstract：:Enhancing immune responses with immune-modulatory monoclonal antibodies directed to inhibitory immune receptors is a promising modality in cancer therapy. Clinical efficacy has been demonstrated with antibodies blocking inhibitory immune checkpoints such as cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) or PD-1/...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Romano E,Kusio-Kobialka M,Foukas PG,Baumgaertner P,Meyer C,Ballabeni P,Michielin O,Weide B,Romero P,Speiser DE

更新日期：2015-05-12 00:00:00

abstract：:It has been reported that expression of familial amyotrophic lateral sclerosis (FALS)-associated mutant Cu/Zn superoxide dismutase-1 (SOD) induces apoptosis of neuronal cells in culture associated with an increase in reactive oxygen species. SOD recently has been shown to prevent calcineurin inactivation, initiating t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee JP,Palfrey HC,Bindokas VP,Ghadge GD,Ma L,Miller RJ,Roos RP

更新日期：1999-03-16 00:00:00

abstract：:The region of human chromosome 22q11 is prone to rearrangements. The resulting chromosomal abnormalities are involved in Velo-cardio-facial and DiGeorge syndromes (VCFS and DGS) (deletions), "cat eye" syndrome (duplications), and certain types of tumors (translocations). As a prelude to the development of mouse models...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Puech A,Saint-Jore B,Funke B,Gilbert DJ,Sirotkin H,Copeland NG,Jenkins NA,Kucherlapati R,Morrow B,Skoultchi AI

更新日期：1997-12-23 00:00:00

abstract：:Cumulative distributions of the number of doublings undergone by mixed megakaryocytic/erythroblastic colonies and by pure megakaryocytic colonies were determined from plasma clot cultures of bone marrow (from C57BL/6 mice) supplemented with erythropoietin. Analysis of these distributions suggests that these colonies a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Paulus JM,Prenant M,Deschamps JF,Henry-Amar M

更新日期：1982-07-01 00:00:00

abstract：:Ler, a homolog of H-NS in enteropathogenic Escherichia coli (EPEC), plays a critical role in the expression of virulence genes encoded by the pathogenic island, locus of enterocyte effacement (LEE). Although Ler acts as an antisilencer of multiple LEE operons by alleviating H-NS-mediated silencing, it represses its ow...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bhat A,Shin M,Jeong JH,Kim HJ,Lim HJ,Rhee JH,Paik SY,Takeyasu K,Tobe T,Yen H,Lee G,Choy HE

更新日期：2014-06-24 00:00:00

abstract：:Nanoparticle-based experimental therapeutics are currently being investigated in numerous human clinical trials. CALAA-01 is a targeted, polymer-based nanoparticle containing small interfering RNA (siRNA) and, to our knowledge, was the first RNA interference (RNAi)-based, experimental therapeutic to be administered to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zuckerman JE,Gritli I,Tolcher A,Heidel JD,Lim D,Morgan R,Chmielowski B,Ribas A,Davis ME,Yen Y

更新日期：2014-08-05 00:00:00

abstract：:Like many physiological ligands, several viruses and toxins enter mammalian cells through receptor-mediated endocytosis. Once internalized, the nucleic acids of several viruses and the toxic subunit of diphtheria toxin gain access to the cytosol of the host cell through an acidic intracellular compartment. In this rep...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Merion M,Schlesinger P,Brooks RM,Moehring JM,Moehring TJ,Sly WS

更新日期：1983-09-01 00:00:00