Abstract:
:We performed microsatellite analysis and tested telomerase activity in paired tissue and urine of bladder cancer patients from frozen archived samples. DNA obtained from microdissected tumor and urine sediment was analyzed and compared to peripheral lymphocytes for microsatellite alterations (loss of heterozygosity [LOH] or instability) using a panel of 20 microsatellite markers in 15 patients with transitional or squamous cell carcinoma of the urinary tract. Additionally, telomerase activity was determined in 12 microdissected tumor specimens and corresponding frozen urine pellets. Tumor cell DNA was detected by microsatellite analysis (LOH or shift) in at least one marker in 14/15 microdissected tumor specimens and in 13/15 DNA samples obtained from urine sediments. Telomerase activity was present in 11/12 tumor samples but could not be detected in any of the corresponding urine sediments. Frozen archived urine samples are useful for retrospective studies utilizing microsatellite analysis or other PCR-based approaches after DNA extraction. However, the evaluation of telomerase protein activity in stored urine samples appears to be unsuitable.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Linn JF,Lango M,Halachmi S,Schoenberg MP,Sidransky Ddoi
10.1002/(sici)1097-0215(19971219)74:6<625::aid-ijcsubject
Has Abstractpub_date
1997-12-19 00:00:00pages
625-9issue
6eissn
0020-7136issn
1097-0215pii
10.1002/(SICI)1097-0215(19971219)74:6<625::AID-IJCjournal_volume
74pub_type
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