A new experimental metastasis model in athymic nude mice, the human malignant melanoma LOX.


:The human tumor line LOX was established as an s.c. xenograft in nude mice from a lymph-node metastasis of a patient with malignant melanoma. I.v. injection into adult nude mice of single-cell suspensions prepared from xenografts resulted in progressively growing lung tumor colonies that killed the animals. No difference in colony formation was seen between cells taken from lung colonies and s.c. xenografts. An in vitro cell line, LOX-L, was established from lung colonies, and the monolayer cells, detached with EDTA, retained the same ability to form experimental lung metastases. In a total of 14 experiments, 82 of 89 mice receiving 1 X 10(6) viable tumor cells died with a mean survival time of 34.1 +/- 4.8 days. Long-term passaging in vivo and in vitro did not result in any alteration of the lung-colonizing potential of the LOX cells, whereas trypsinization of the cells before i.v. injection reduced lung colony formation. The life span was inversely related to the number of LOX cells injected, permitting estimation of the cell kill caused by chemotherapy. Mice injected i.v. with the LOX cells showed the same relative response to cis-diamminedichloroplatinum (CDDP) and mitozolomide (MZA) as did animals carrying s.c. xenografts. The LOX cells have shown a remarkable stability and similarity to the cells of the patient's tumor with respect to morphology, karyotype and chemosensitivity. The LOX model may be useful for testing effects of therapy on lung micro- and macrometastases, and the activity of antimetastatic agents, as well as for studying mechanisms involved in the metastatic process.


Int J Cancer


Fodstad O,Aamdal S,McMenamin M,Nesland JM,Pihl A




Has Abstract


1988-03-15 00:00:00












  • Kinetics of the immune response of tumor-bearing hamsters to two simian virus 40 coded non-structural polypeptides present in simian virus 40 tumor cells.

    abstract::The kinetics of the immune response of hamsters transplanted with virus-free SV40 tumor cells (TSV5) to two SV40-coded non-structural proteins of 94,000 daltons (T antigen) and 17,000 daltons (t antigen) was studied in order to determine if the variation in the immune reactivity of serum from tumor-bearing animals tow...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Ransom JH,Thompson DL,Tevethia SS

    更新日期:1982-02-15 00:00:00

  • Acrylamide exposure measured by food frequency questionnaire and hemoglobin adduct levels and prostate cancer risk in the Cancer of the Prostate in Sweden Study.

    abstract::Acrylamide, a probable human carcinogen, is formed during the cooking of many commonly consumed foods. Data are scant on whether dietary acrylamide represents an important cancer risk in humans. We studied the association between acrylamide and prostate cancer risk using 2 measures of acrylamide exposure: intake from ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Wilson KM,Bälter K,Adami HO,Grönberg H,Vikström AC,Paulsson B,Törnqvist M,Mucci LA

    更新日期:2009-05-15 00:00:00

  • Loss of EphA2 receptor tyrosine kinase reduces ApcMin/+ tumorigenesis.

    abstract::The Eph receptor A2 (EphA2) is overexpressed in a range of human epithelial cancers, a phenotype that is associated with cancer cell proliferation, progression and angiogenesis. Mouse models of mammary neoplasia have confirmed the role of EphA2 as mice carrying a knockout allele of EphA2 were resistant to breast cance...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Bogan C,Chen J,O'Sullivan MG,Cormier RT

    更新日期:2009-03-15 00:00:00

  • Cytoskeleton and ECM tumor mutant peptides: Increased protease sensitivities and potential consequences for the HLA class I mutant epitope reservoir.

    abstract::Cytoskeleton and extracellular matrix-related proteins (CECMPs) represent the most common class of cancer mutants, owing to the large size of their coding regions and the randomness of mutagenesis. We used a bioinformatics approach to assess the impact of amino acid (AA) substitutions on the sensitivity of CECMPs to p...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Callahan BM,Patel JS,Fawcett TJ,Blanck G

    更新日期:2018-03-01 00:00:00

  • MicroRNA-34b functions as a potential tumor suppressor in endometrial serous adenocarcinoma.

    abstract::Endometrial serous adenocarcinoma (ESC) is aggressive and carries a poor prognosis. p53 is frequently mutated in ESC. microRNAs (miRNAs) are a direct p53 target and have been implicated in cancer cell behavior. In this study, we compared miRNA expression levels in ESC with the levels in endometrial endometrioid adenoc...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Hiroki E,Suzuki F,Akahira J,Nagase S,Ito K,Sugawara J,Miki Y,Suzuki T,Sasano H,Yaegashi N

    更新日期:2012-08-15 00:00:00

  • Elevated expression of ICAM1 (CD54) and minimal expression of LFA3 (CD58) in Epstein-Barr-virus-positive nasopharyngeal carcinoma cells.

    abstract::Undifferentiated nasopharyngeal carcinoma (NPC) is a remarkable entity among human tumors because of its constant association with the Epstein-Barr virus (EBV). Malignant epithelial cells harbor the EBV genome and often express at least 2 species of latent EBV protein (EBNA1 and LMP1). Despite the massive presence of ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Busson P,Zhang Q,Guillon JM,Gregory CD,Young LS,Clausse B,Lipinski M,Rickinson AB,Tursz T

    更新日期:1992-04-01 00:00:00

  • Cloning and expression of the recombinant FAb fragment of monoclonal antibody K1 that reacts with mesothelin present on mesotheliomas and ovarian cancers.

    abstract::The monoclonal antibody (MAb) K1 recognizes an approximate 40 kDa glycoprotein, mesothelin, that is present on the surface of human mesothelial cells, mesotheliomas and ovarian cancers. We have cloned the cDNAs encoding the variable regions of MAb K1 and constructed plasmids for expression of recombinant K1(FAb). Reco...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Brinkmann U,Webber K,Di Carlo A,Beers R,Chowdhury P,Chang K,Chaudhary V,Gallo M,Pastan I

    更新日期:1997-05-16 00:00:00

  • Detection of human T-lymphotropic virus type-I DNA and mRNA in the lymph nodes; using polymerase chain reaction in situ hybridization (PCR/ISH) and reverse transcription (RT-PCR/ISH).

    abstract::To examine the relationship between human T-lymphotrophic virus type I (HTLV-I) proviral DNA and its expression in the lymph nodes, HTLV-I DNA and tax/rex mRDA were directly amplified by polymerase chain reaction in situ hybridization (PCR/ISH), and reverse transcription (RT)-PCR/ISH [RT-PCR/ISH]. We studied 24 lymph ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Ohshima K,Suzumiya J,Izumo S,Mukai Y,Tashiro K,Kikuchi M

    更新日期:1996-03-28 00:00:00

  • Rectal cancer and occupational risk factors: a hypothesis-generating, exposure-based case-control study.

    abstract::In 1979, a hypothesis-generating, population-based case-control study was undertaken in Montreal, Canada, to explore the association between occupational exposure to 294 substances, 130 occupations and industries, and various cancers. Interviews were carried out with 3, 630 histologically confirmed cancer cases, of wh...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Dumas S,Parent ME,Siemiatycki J,Brisson J

    更新日期:2000-09-15 00:00:00

  • Antibody responses to Herpesvirus papio antigens in baboons with lymphoma.

    abstract::An Epstein-Barr virus-related herpesvirus, termed Herpesvirus papio (HVP), was isolated from baboons (Papio hamadryas) at the Institute of Experimental Pathology and Therapy, Sukhumi, USSR, where there is a continuing outbreak of lymphoma. In the present study sera from diseased baboons and from age- and sex-matched c...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Neubauer RH,Rabin H,Strnad BC,Lapin BA,Yakovleva LA,Indzie E

    更新日期:1979-02-01 00:00:00

  • Progression to androgen-independent LNCaP human prostate tumors: cellular and molecular alterations.

    abstract::Lethal phenotypes of human prostate cancer are characterized by progression to androgen-independence and metastasis. For want of a clinically relevant animal model, mechanisms behind this progression remain unclear. Our study used an in vivo model of androgen-sensitive LNCaP human prostate cancer cell xenografts in ma...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Zhou JR,Yu L,Zerbini LF,Libermann TA,Blackburn GL

    更新日期:2004-07-20 00:00:00

  • cagA-positive Helicobacter pylori and risk for developing gastric carcinoma in Brazil.

    abstract::To evaluate a possible association between infection with cag A-positive strains and gastric carcinoma increased risk we studied 119 Helicobacter pylori-positive patients with gastric carcinoma and 119 matched controls. The presence of cag A gene was investigated by PCR in H. pylori isolates and in gastric biopsy spec...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Queiroz DM,Mendes EN,Rocha GA,Oliveira AM,Oliveira CA,Magalhães PP,Moura SB,Cabral MM,Nogueira AM

    更新日期:1998-10-05 00:00:00

  • Paracrine sonic hedgehog signaling contributes significantly to acquired steroidogenesis in the prostate tumor microenvironment.

    abstract::Despite the substantial benefit of androgen deprivation therapy (ADT) for metastatic prostate cancer, patients often progress to castration-resistant disease (CRPC) that is more difficult to treat. CRPC is associated with renewed androgen receptor activity in tumor cells and restoration of tumor androgen levels throug...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Lubik AA,Nouri M,Truong S,Ghaffari M,Adomat HH,Corey E,Cox ME,Li N,Guns ES,Yenki P,Pham S,Buttyan R

    更新日期:2017-01-15 00:00:00

  • Eurogin roadmap 2017: Triage strategies for the management of HPV-positive women in cervical screening programs.

    abstract::Cervical cancer screening will rely, increasingly, on HPV testing as a primary screen. The requirement for triage tests which can delineate clinically significant infection is thus prescient. In this EUROGIN 2017 roadmap, justification behind the most evidenced triages is outlined, as are challenges for implementation...

    journal_title:International journal of cancer

    pub_type: 杂志文章,评审


    authors: Cuschieri K,Ronco G,Lorincz A,Smith L,Ogilvie G,Mirabello L,Carozzi F,Cubie H,Wentzensen N,Snijders P,Arbyn M,Monsonego J,Franceschi S

    更新日期:2018-08-15 00:00:00

  • Glycoprotein 90K/MAC-2BP interacts with galectin-1 and mediates galectin-1-induced cell aggregation.

    abstract::The glycoprotein 90K was originally described as a tumor-secreted antigen and subsequently found to have immunostimulatory activity as well as other possible functions. This protein interacts with an endogenous lectin, galectin-3, and may play a role in tumor metastasis through this interaction. Because 90K is heavily...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Tinari N,Kuwabara I,Huflejt ME,Shen PF,Iacobelli S,Liu FT

    更新日期:2001-01-15 00:00:00

  • Patterns of growth and differentiation in the colon carcinoma cell line LIM 1863.

    abstract::The LIM 1863 colon carcinoma cell line grows in the form of morphologically and functionally organized organoids. Cells are arranged around a central lumen with a brush border and nuclei are polarized to the periphery. The organoids contain 3 morphological cell types (columnar, goblet and caveolated cells). By agar cl...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Hayward IP,Whitehead RH

    更新日期:1992-03-12 00:00:00

  • Immortalization of oral keratinocytes by functional inactivation of the p53 and pRb pathways.

    abstract::A subgroup of head and neck squamous cell carcinomas (HNSCCs) contains high-risk human papillomavirus-type 16 (HPV16). The viral E6 and E7 oncoproteins inactivate the p53 and pRb proteins, respectively. We examined the causative effect of HPV16 E6 and E7 expression on the immortalization of normal oral keratinocytes (...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Smeets SJ,van der Plas M,Schaaij-Visser TB,van Veen EA,van Meerloo J,Braakhuis BJ,Steenbergen RD,Brakenhoff RH

    更新日期:2011-04-01 00:00:00

  • Establishment of a hepatocellular carcinoma patient-derived xenograft platform and its application in biomarker identification.

    abstract::Using a method optimized in hepatocellular carcinoma (HCC), we established patient-derived xenograft (PDX) models with an increased take rate (42.2%) and demonstrated that FBS +10% dimethyl sulfoxide exhibited the highest tumor take rate efficacy. Among 254 HCC patients, 103 stably transplantable xenograft lines that ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Hu B,Li H,Guo W,Sun YF,Zhang X,Tang WG,Yang LX,Xu Y,Tang XY,Ding GH,Qiu SJ,Zhou J,Li YX,Fan J,Yang XR

    更新日期:2020-03-15 00:00:00

  • Statins revert doxorubicin resistance via nitric oxide in malignant mesothelioma.

    abstract::Human malignant mesothelioma (HMM) is resistant to many anticancer drugs, including doxorubicin. Mevastatin and simvastatin, 2 inhibitors of 3-hydroxy-3-methylglutaryl coenzyme A (HMGCoA) reductase, potentiated the intracellular accumulation and the cytotoxicity of doxorubicin in HMM cells constitutively expressing P-...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Riganti C,Orecchia S,Pescarmona G,Betta PG,Ghigo D,Bosia A

    更新日期:2006-07-01 00:00:00

  • Occurrence of anticancer activity of prednisolone via adrenalectomy and inhibition of adrenaline in rats.

    abstract::In this study, the anticancer effect of prednisolone was investigated using rats with normal endogen adrenaline levels (intact), reduced adrenaline levels (metyrosine-induced) and adrenaline deficiency (adrenalectomized) via gastric adenocarcinoma model. Gastric adenocarcinoma was induced with N-methyl-N'-nitro-N-nitr...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Suleyman H,Cadirci E,Albayrak A,Halici Z,Gundogdu C,Hacimuftuoglu A

    更新日期:2010-04-01 00:00:00

  • Plasma microRNAs as biomarkers of pancreatic cancer risk in a prospective cohort study.

    abstract::Noninvasive biomarkers for early pancreatic ductal adenocarcinoma (PDAC) diagnosis and disease risk stratification are greatly needed. We conducted a nested case-control study within the Prospective Investigation into Cancer and Nutrition (EPIC) cohort to evaluate prediagnostic microRNAs (miRs) as biomarkers of subseq...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Duell EJ,Lujan-Barroso L,Sala N,Deitz McElyea S,Overvad K,Tjonneland A,Olsen A,Weiderpass E,Busund LT,Moi L,Muller D,Vineis P,Aune D,Matullo G,Naccarati A,Panico S,Tagliabue G,Tumino R,Palli D,Kaaks R,Katzke VA,

    更新日期:2017-09-01 00:00:00

  • Positive correlation between p27Kip1 expression and progression of human esophageal squamous cell carcinoma.

    abstract::p27Kip1, one of the cyclin-dependent kinase (CDK) inhibitors (CDKIs), blocks progression from G1 to S phase by binding cyclin D1-CDK4 and/or cyclin E-CDK2 and inhibiting their activities. Reflecting the function of p27 as a CDKI in vitro, a reduced expression of protein p27 has recently been reported to be associated ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Anayama T,Furihata M,Ishikawa T,Ohtsuki Y,Ogoshi S

    更新日期:1998-08-21 00:00:00

  • Genetic variants in RUNX3, AMD1 and MSRA in the methionine metabolic pathway and survival in nonsmall cell lung cancer patients.

    abstract::Abnormal methionine dependence in cancer cells has led to methionine restriction as a potential therapeutic strategy. We hypothesized that genetic variants involved in methionine-metabolic genes are associated with survival in nonsmall cell lung cancer (NSCLC) patients. Therefore, we investigated associations of 16,37...

    journal_title:International journal of cancer

    pub_type: 杂志文章,meta分析


    authors: Chen K,Liu H,Liu Z,Luo S,Patz EF Jr,Moorman PG,Su L,Shen S,Christiani DC,Wei Q

    更新日期:2019-08-01 00:00:00

  • CDKN2A/p16 inactivation in the prognosis of oligodendrogliomas.

    abstract::The cell-cycle regulator p16 inhibits the complex cdk4-cyclin D1 and controls G1-S transition. In human tumors, p16 inactivation is often accomplished by homozygous deletion (HD) of its encoding gene, CDKN2A. Methylation of the 5' CpG island promoter has been proposed as an alternative mechanism of inactivation. Expre...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Bortolotto S,Chiadò-Piat L,Cavalla P,Bosone I,Chiò A,Mauro A,Schiffer D

    更新日期:2000-11-15 00:00:00

  • Further characterization of a herpesvirus-positive orang-utan cell line and comparative aspects of in vitro transformation with lymphotropic old world primate herpesviruses.

    abstract::An orang-utan (Pongo pygmaeus) suspension line, CP81, was shown to lack myeloid markers of lysozyme activity an d phagocytosis but to be positive for lymphocytic N-alkaline phosphatase activity, and to release a B-cell-tropic herpesvirus. This herpesvirus, termed Herpesvirus pongo, had 30--40% DNA homology with EBV an...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Rabin H,Neubauer RH,Hopkins RF 3rd,Nonoyama M

    更新日期:1978-06-15 00:00:00

  • Analysis of HLA antigen expression in benign and malignant melanocytic lesions reveals that upregulation of HLA-G expression correlates with malignant transformation, high inflammatory infiltration and HLA-A1 genotype.

    abstract::Previous studies indicate that the nonclassical class I HLA-G antigen, whose physiologic expression is mainly restricted to placenta, is upregulated in melanoma, renal carcinoma, lung carcinoma, glioblastoma and ovarian carcinoma, where its inhibitory effect on cytotoxic effector cells function is thought to participa...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Ibrahim EC,Aractingi S,Allory Y,Borrini F,Dupuy A,Duvillard P,Carosella ED,Avril MF,Paul P

    更新日期:2004-01-10 00:00:00

  • Consistent expression of the stem cell renewal factor BMI-1 in primary and metastatic melanoma.

    abstract::Stem cell-like cells have recently been identified in melanoma cell lines, but their relevance for melanoma pathogenesis is controversial. To characterize the stem cell signature of melanoma, expression of stem cell markers BMI-1 and nestin was studied in 64 cutaneous melanomas, 165 melanoma metastases as well as 53 m...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Mihic-Probst D,Kuster A,Kilgus S,Bode-Lesniewska B,Ingold-Heppner B,Leung C,Storz M,Seifert B,Marino S,Schraml P,Dummer R,Moch H

    更新日期:2007-10-15 00:00:00

  • Expression of sperm protein 17 (Sp17) in ovarian cancer.

    abstract::Sperm protein 17 (Sp17) is an antigenic protein highly expressed in spermatozoa. Sp17 expression was demonstrated recently in multiple myeloma, suggesting that it may be a novel cancer-testis antigen. Expression of Sp17 mRNA and protein was examined in human ovarian tumors. Sp17 mRNA was evaluated by reverse transcrip...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Straughn JM Jr,Shaw DR,Guerrero A,Bhoola SM,Racelis A,Wang Z,Chiriva-Internati M,Grizzle WE,Alvarez RD,Lim SH,Strong TV

    更新日期:2004-03-01 00:00:00

  • Differential utilization of the SV40 enhancer and early promoter in various human lung carcinoma cell lines.

    abstract::Cell lines derived from the major morphological types of human lung cancer were tested for their ability to utilize the SV40 enhancer/early promoter. These cell lines were transfected with a recombinant plasmid containing a reporter gene, coding for chloramphenicol acetyltransferase (CAT), under the control of the SV4...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Wong YC,Bernal SD

    更新日期:1990-05-15 00:00:00

  • Human papillomavirus-16 DNA methylation patterns support a causal association of the virus with oral squamous cell carcinomas.

    abstract::Infection with human papillomavirus-16 (HPV-16) is the cause of most anogenital carcinomas. This virus is also detected in about 20% of all head and neck squamous cell carcinomas. While there is strong evidence for a causal etiological role in the case of tonsillar carcinomas, causal association with malignant lesions...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Balderas-Loaeza A,Anaya-Saavedra G,Ramirez-Amador VA,Guido-Jimenez MC,Kalantari M,Calleja-Macias IE,Bernard HU,Garcia-Carranca A

    更新日期:2007-05-15 00:00:00