Abstract:
:We demonstrate herein the ability of transforming growth factor-beta-2 (TGFbeta2) to potently activate extracellular signal-regulated kinase 2 (ERK2) in the highly TGFbeta-sensitive breast cancer cell (BCC) line Hs578T. The ERK2 isoform was activated by 3-fold within 5 min of TGFbeta2 addition to Hs578T cells. However, TGFbeta2 only slightly activated ERK2 (1.5-fold) in the partially TGFbeta-responsive BCC line MDA-MB-23 1. The magnitude of the difference in activation of ERK2 by TGFbeta2 in the two cell lines paralleled the difference in the IC50 values for TGFbeta inhibition of DNA synthesis; the IC50 value in the MDA-MB-231 cells was 32-fold greater than that in the Hs578T cells. Further, our data demonstrate that TGFbeta2 activated the stress-activated protein kinase/Jun N-terminal kinase (SAPK/JNK) type of mitogen-activated protein kinases (MAPKs); maximal induction levels were 2.5-fold above basal values and were attained at 30 min after TGFbeta2 treatment. Transient co-transfection of a luciferase reporter construct (3TP-Lux) containing three AP-1 sites and the plasminogen activator inhibitor-1 (PAI-1) promoter, in conjunction with a construct that directs expression of a dominant-negative mutant ERK2 (TAYF) protein, did not block the ability of TGFbeta to induce AP-1 or PAI-1 activity. In contrast, TAYF ERK2 was able to block EGF and insulin-induced 3TP-Lux-reporter activity. These results indicate that in these BCCs, the activation of ERK2 by TGFbeta is more tightly linked to the ability of TGFbeta to inhibit DNA synthesis than to the ability to stimulate promoter regions important for TGFbeta production and control of the extracellular matrix. In addition, this is the first demonstration that TGFbeta can activate the SAPK/JNK type of MAPK in TGFbeta-sensitive human BCCs.
journal_name
Cancer Lettjournal_title
Cancer lettersauthors
Frey RS,Mulder KMdoi
10.1016/s0304-3835(97)00211-5subject
Has Abstractpub_date
1997-07-15 00:00:00pages
41-50issue
1eissn
0304-3835issn
1872-7980pii
S0304-3835(97)00211-5journal_volume
117pub_type
杂志文章相关文献
CANCER LETTERS文献大全abstract::In previous studies, we confirmed that mitofusin-2 (Mfn2) induced apoptosis in hepatocellular carcinoma (HCC) cells. However, the exact molecular mechanism remained unclear. Mfn2 expressed lower in tumour tissues, compared with adjacent non-cancer tissues. Furthermore, Mfn2 immunostaining was very weak in HCC tissue (...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2014.12.025
更新日期:2015-03-01 00:00:00
abstract::The South American opossum, Monodelphis domestica, has been used as a model system to study ultraviolet (UV)-induced genetic alterations that lead to the development of melanoma. Suckling young of Monodelphis exposed to multiple doses of UVB radiation can develop benign or malignant melanomas later as adults. Point mu...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(01)00783-2
更新日期:2002-10-08 00:00:00
abstract::Bile acids are promoters of colon cancer; however, the mechanism(s) of action of this tumor promoter are largely unknown. Bile acids induce apoptosis in colon epithelial cells and it is probable that the modulation of apoptosis contributes, in part, to colon carcinogenesis. We tested the hypothesis that damage to mito...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(01)00786-8
更新日期:2002-03-28 00:00:00
abstract::Emerging evidence indicates that cancer stem cells (CSCs) are involved in tumorigenesis, tumor recurrence, and therapeutic resistance in hepatocellular carcinoma (HCC). However, the mechanisms underlying HCC CSC regulation remain largely unknown. Here we report our analysis of 97 paraffin-embedded HCC tumor specimens....
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2019.02.040
更新日期:2019-05-28 00:00:00
abstract::Although pectenotoxin-2 (PTX-2) is known to modify the actin cytoskeleton, very little is known about its apoptosis mechanism. In this study, we investigated whether PTX-2 induces apoptotic effects through suppression of the NF-kappaB signaling pathway in several leukemia cell types. PTX-2 significantly induced growth...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2008.05.034
更新日期:2008-11-18 00:00:00
abstract::To minimize adverse side effects of chemotherapy, we have developed a micellar drug carrier that retains hydrophobic drugs, and then releases the drug by ultrasonic stimulation. This study investigated the DNA damage induced by doxorubicin (DOX) delivered to human leukemia (HL-60) cells from pluronic P-105 micelles wi...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(00)00399-2
更新日期:2000-06-30 00:00:00
abstract::Neoxanthin and fucoxanthin, which have the characteristic structure of 5,6-monoepoxide and an allenic bond, were previously found to reduce the viability of human prostate cancer cells most intensively among 15 dietary carotenoids tested. In the present study, the induction of apoptosis in PC-3 cells by these two caro...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2004.07.048
更新日期:2005-03-18 00:00:00
abstract::Growing evidence from epidemiologic and preclinical studies suggests that nonsteroidal anti-inflammatory drugs (NSAIDs) reduce the risk of gastrointestinal (GI) cancers, including esophageal, gastric, pancreatic, colorectal cancer, and hepatocellular carcinoma. However, there is also evidence indicating the absence of...
journal_title:Cancer letters
pub_type: 杂志文章,评审
doi:10.1016/j.canlet.2013.09.001
更新日期:2014-04-10 00:00:00
abstract::Non-small cell lung cancer (NSCLC) patients with activating EGFR mutations are often successfully treated with EGFR tyrosine kinase inhibitor (TKI) such as erlotinib; however, treatment resistance inevitably occurs. Given tumor metabolism of glucose and therapeutic response are intimately linked, we explored the metab...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2018.07.025
更新日期:2018-10-10 00:00:00
abstract::Mantle cell lymphoma (MCL) is an aggressive and incurable mature B cell neoplasm. The current treatments are based on chemotherapeutics and new class of drugs (e.g. Ibrutinib(®)), which in most cases ends with tumor resistance and relapse. Therefore, further development of novel therapeutic modalities is needed. Exoso...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2015.04.026
更新日期:2015-08-01 00:00:00
abstract::AKT is a promising target for anticancer drug development. In this work, a bioinformatics approach was applied to search for AKT inhibitors based on the correlation analysis between phospho-Ser473 AKT expression level and the antiproliferative data of NCI small molecule compounds against NCI 60 cancer cell lines, the ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2006.12.035
更新日期:2007-07-18 00:00:00
abstract::Glioblastoma multiforme (GBM) is the most common malignant brain tumor, which remains incurable. Plant extracts are a potential source of potent anticancer medicines. In this study, we investigated the effect of isolinderalactone from Lindera aggregata on tumor growth using U-87 human glioblastoma cells. Treatment wit...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2018.11.027
更新日期:2019-02-28 00:00:00
abstract::Fibronectin (FN), a heterodimeric glycoprotein overexpressed in several types of tumors, has been implicated in cancer progression via the activation of integrin-mediated pro-oncogenic pathways. The FN level in human bile fluid is dramatically increased in malignant biliary diseases; however, FN expression and its bio...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2015.01.041
更新日期:2015-05-01 00:00:00
abstract::High doses of folate and its quinazoline analogue with antitumour activity, N10-propargyl-5,8-dideazafolic acid (CB 3717), caused severe renal damage in mice, leading in the case of folate to death. The mouse kidneys increased in weight, which was accompanied by time- and dose-dependent induction of ornithine decarbox...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(91)90147-a
更新日期:1991-09-01 00:00:00
abstract::Vitamin C has been suggested and disputed as an anti-cancer agent. Previous in vitro studies using either primary cell cultures from cancer patients or tumor cell lines have suggested that tumor cells with different lineages may have different sensitivities to ascorbic acid. In this study we report characterization of...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(93)90080-s
更新日期:1993-06-15 00:00:00
abstract::We investigated the anticancer activity of erufosine in oral squamous carcinoma cell lines in terms of cell proliferation, colony formation, induction of autophagy/apoptosis, cell cycle and mTOR signaling pathway. Erufosine showed dose-dependent cytotoxicity in all cell lines, it induced autophagy as well as apoptosis...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2011.12.032
更新日期:2012-06-01 00:00:00
abstract::Tissue samples from germ-free and conventional rats fed pectins with different degrees of esterification (DE) were tested for containing indigenous DNA adducts (I-compounds). For 21 days groups of six germ-free and 10 conventional rats each were fed either pectin-free or with diets containing 7.5% of three differently...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(97)04621-1
更新日期:1997-03-19 00:00:00
abstract::Patients with Her2-positive breast cancer exhibit de novo resistance or develop acquired resistance in less than one year after Her2 targeting treatment, but the mechanism is not fully elucidated. Compensatory pathways such as the IGF-1R/IRS-1 pathway, are activated, leading to aberrant enhanced PI3K/Akt/mTOR pathway ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2019.08.008
更新日期:2019-11-01 00:00:00
abstract::Rectal biopsies and fecal collections were obtained from a consecutive series of 34 outpatients prior to colonoscopy at a gastroenterology clinic. Subsequently, 14 were found to have no colonic pathology, 13 had adenomatous polyps, (3 of those had a previous history of colon cancer) and 7 were diagnosed with colon can...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(88)90023-7
更新日期:1988-01-01 00:00:00
abstract::Ellagic acid (EA) is a polyphenol found in several plants and fruits. The objectives of this study were to examine the molecular mechanisms by which EA inhibits pancreatic cancer growth in Balb C nude mice. PANC-1 cells were injected subcutaneously into Balb c nude mice, and tumor-bearing mice were treated with EA. Th...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2013.05.009
更新日期:2013-09-01 00:00:00
abstract::Loss of heterozygosity (LOH) is an important event of tumorigenesis. In this paper, we report the comprehensive LOH analyses with microsatellite markers and their results at chromosome 8p11.1-q13.3 in gastric cancer. The microsatellite markers D8S2323 and D8S2330 exhibited high LOH frequencies, 54.2 and 57.1%, respect...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2004.03.041
更新日期:2004-09-15 00:00:00
abstract::By virtue of being a localized treatment modality, radiotherapy is unable to deliver a tumoricidal radiation dose to tissues outside of the irradiated field. Nevertheless, ionizing radiation may result in radiation damage mediated by a bystander like effect away from the irradiated field, but this response is likely t...
journal_title:Cancer letters
pub_type: 杂志文章,评审
doi:10.1016/j.canlet.2015.04.007
更新日期:2015-11-28 00:00:00
abstract::The present study was undertaken to determine the kinetics of DNA synthesis and expression of cell cycle dependent proto-oncogenes in response to two types of cell proliferative stimuli in male Wistar rat liver. The peak of DNA synthesis was approximately 24 h after a compensatory cell proliferative stimulus induced b...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(89)90186-9
更新日期:1989-09-15 00:00:00
abstract::Vemurafenib, a BRAF inhibitor, elicits in ∼80% of BRAFV600E-mutant melanoma patients a transient anti-tumor response which precedes the emergence of resistance. We tested whether an acidic tumor microenvironment may favor a BRAF inhibitor resistance. A375M6 BRAFV600E melanoma cells, either exposed for a short period o...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2017.08.010
更新日期:2017-11-01 00:00:00
abstract::We studied chromosome 1p loss of heterozygosity (1p-LOH) in 53 neuroblastomas (NBs) using 15 (CA)n repeat loci, which covered a region of 90 cM. We also assessed chromosome 1p36 deletion by fluorescence in situ hybridization (FISH) on interphase nuclei. 1p-LOH was found in 19 (36%, 95% confidence interval (CI) 23-50%)...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/s0304-3835(98)00122-0
更新日期:1998-08-14 00:00:00
abstract::Tumors are highly heterogeneous at the patient, tissue, cellular, and molecular levels. This multi-scale heterogeneity poses significant challenges for effective therapies, which ideally must not only distinguish between tumorous and healthy tissue, but also fully address the wide variety of tumorous sub-clones. Commo...
journal_title:Cancer letters
pub_type: 杂志文章,评审
doi:10.1016/j.canlet.2015.12.019
更新日期:2016-09-28 00:00:00
abstract::Recent studies show that metabolic patterns typical of cancer cells, including aerobic glycolysis and increased lipogenesis, are not unique to malignancy, but rather originate in physiologic development. In the postnatal brain, where sufficient oxygen for energy metabolism is scrupulously maintained, neural progenitor...
journal_title:Cancer letters
pub_type: 杂志文章,评审
doi:10.1016/j.canlet.2014.02.017
更新日期:2015-01-28 00:00:00
abstract::Gastric adenocarcinoma and esophageal adenocarcinoma are aggressive cancers with a poor prognosis. Therefore, new therapeutic strategies are needed, especially for patients refractory to conventional treatment. Cancer immunotherapy (CIT), is a promising new treatment option and is effective in a proportion of patients...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/j.canlet.2018.11.001
更新日期:2019-02-01 00:00:00
abstract::Extracts of leukemic lymphocytes and leukemic myelomonocytic cells bind significantly more cAMP/mg protein than do normal cells. The binding of cAMP in leukemic cells is non-specific since the binders are unable to effectively discriminate between cAMP and cGMP in contrast to normal cells. When normal lymphocytes are ...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(80)90004-x
更新日期:1980-01-01 00:00:00
abstract::Insertion of functional interleukin-2 (IL-2) gene into a plasmacytoma cell line X63-Ag8.653 substantially reduced tumorigenicity of the resulting cloned cells, designated as X63-m-IL-2. Peritumoral administration of the X63-m-IL-2 cells, producing constitutively large quantities of IL-2, resulted in regressions of est...
journal_title:Cancer letters
pub_type: 杂志文章
doi:10.1016/0304-3835(92)90104-4
更新日期:1992-03-15 00:00:00