Abstract:
:We have provided evidence that mitochondrial membrane permeability transition induced by inorganic phosphate, uncouplers or prooxidants such as t-butyl hydroperoxide and diamide is caused by a Ca(2+)-stimulated production of reactive oxygen species (ROS) by the respiratory chain, at the level of the coenzyme Q. The ROS attack to membrane protein thiols produces cross-linkage reactions, that may open membrane pores upon Ca2+ binding. Studies with submitochondrial particles have demonstrated that the binding of Ca2+ to these particles (possibly to cardiolipin) induces lipid lateral phase separation detected by electron paramagnetic resonance experiments exploying stearic acids spin labels. This condition leads to a disorganization of respiratory chain components, favoring ROS production and consequent protein and lipid oxidation.
journal_name
Biosci Repjournal_title
Bioscience reportsauthors
Vercesi AE,Kowaltowski AJ,Grijalba MT,Meinicke AR,Castilho RFdoi
10.1023/a:1027335217774subject
Has Abstractpub_date
1997-02-01 00:00:00pages
43-52issue
1eissn
0144-8463issn
1573-4935journal_volume
17pub_type
杂志文章,评审abstract::Canstatin, the non-collagenous domain of collagen type IV α-chains, belongs to a series of collagen-derived angiogenic inhibitors. In this study, the inhibitory effect of recombinant canstatin on tumour growth was investigated using a gastric cancer xenograft model. The volume and weight of tumours in mice treated wit...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20140012
更新日期:2014-04-01 00:00:00
abstract::HIV-1 infects host cells by sequential interactions of its fusion protein (gp120-gp41) with receptors CD4, CXCR4 and/or CCR5 followed by fusion of viral and host membranes. Studies indicate that additional factors such as receptor density and composition of viral and cellular lipids can dramatically modulate the fusio...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1007/s10540-005-2894-5
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abstract::Objective: To explore whether aspirin (ASA) enhances the sensitivity of hepatocellular carcinoma (HCC) side population (SP) cells to doxorubicin (Doxo) via miR-491/ATP-binding cassette sub-family G member 2 (ABCG2).Methods: Non-SP and SP cells were isolated from MHCC-97L cell line using flow cytometry analysis and flu...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20180854
更新日期:2018-11-09 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20080145
更新日期:2009-08-01 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20194202
更新日期:2020-02-28 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1007/BF01788370
更新日期:1995-10-01 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1023/a:1025576307912
更新日期:2003-04-01 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20193796
更新日期:2020-06-26 00:00:00
abstract:RATIONALE:Houshiheisan (HSHS), a classic prescription in traditional Chinese medicine (TCM), has remarkable efficacy in the treatment of ischemic stroke. OBJECTIVE:To investigate the pro-angiogenic effect and molecular mechanism of HSHS for stroke recovery. METHODS AND RESULTS:The rat permanent middle cerebral artery...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20191006
更新日期:2019-10-30 00:00:00
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journal_title:Bioscience reports
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journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1007/s10540-005-2848-y
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abstract::A possible role for Ca2+ and calmodulin in the action of growth-hormone-releasing factor (GHRF) was investigated. Low extracellular Ca2+ (less than 100 microM), methoxyverapamil, flunarizine, cinnarizine, and Co2+ decreased both basal and GHRF-stimulated growth-hormone secretion, but did not totally inhibit GHRF-stimu...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116691
更新日期:1984-12-01 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章,meta分析
doi:10.1042/BSR20171626
更新日期:2018-07-06 00:00:00
abstract::Secretion of high molecular weight (HMW) adiponectin is dependent on post-translational modification (PTM) of conserved lysines in the collagenous domain. The present study aims to characterize the enzymes responsible for the PTM of conserved lysines which leads to HMW adiponectin secretion, and to define its signific...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20170105
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abstract::Polymorphisms of methylenetetrahydrofolate reductase (MTHFR) in hormone metabolism pathways might cause metabolic disturbances and contribute to the development of polycystic ovary syndrome (PCOS) and ovarian cancer, but the published studies were inconsistent. The aim of the present study was to evaluate the MTHFR C6...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20200995
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20180710
更新日期:2018-11-09 00:00:00
abstract::miRNAs are non-coding RNA molecules typically 18-22 nucleotides long that can suppress the expression of their target genes. Several laboratories have attempted to identify miRNAs from the pig that are involved in Salmonella infection. These bioinformatics strategies using the newly available genomic sequence are gene...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20160243
更新日期:2016-08-31 00:00:00
abstract::The family of 5-HT4 receptors comprises 16 putative splice variants. We have previously shown that there are differences in signal transduction of the h5-HT(4a) and h5-HT(4b) receptors. In the present study, the internalization of these two splice variants following receptor stimulation was investigated with confocal ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/s10540-005-2582-5
更新日期:2004-06-01 00:00:00
abstract::In the course of evolution, mitochondria lost their independence, and mtDNA became "slave" of nDNA, depending on numerous nucleus-encoded factors for its integrity, replication and expression. Mutations in any of these factors may alter the cross-talk between the two genomes and cause diseases that affect mtDNA integr...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1007/s10540-007-9036-1
更新日期:2007-06-01 00:00:00
abstract:BACKGROUND:Disc degeneration is correlated with mechanical load. Osteogenic protein-1 (OP-1) is potential to regenerate degenerative disc. OBJECTIVE:To investigate whether OP-1 can protect against high magitude compression-induced nucleus pulposus (NP) cell apoptosis and NP matrix catabolism, and its potential mechani...
journal_title:Bioscience reports
pub_type: 杂志文章
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更新日期:2018-02-13 00:00:00
abstract::Recent developments in the study of peptide amidation are reviewed. The main areas covered are assay procedures, purification of amidating enzymes, co-factors and regulation, mechanism and specificity of the amidating reaction, and multiple forms of the amidating enzyme and glycosylation. Discussion is presented on as...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1007/BF01122123
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116191
更新日期:1981-05-01 00:00:00
abstract::Neuroblastoma, which accounts for approximately 10% of all pediatric cancer-related deaths, has become a therapeutic challenge and global burden attributed to poor outcomes and mortality rates of its high-risk form. Previous genome-wide association studies (GWASs) identified the LINC00673 rs11655237 C>T polymorphism t...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20171667
更新日期:2018-02-08 00:00:00
abstract::Matrix-bound monoclonal antibodies against urokinase have been used to purify this enzyme by affinity chromatography. In a single-step procedure, urokinase can be isolated from crude preparations with high yield and high purity, and without loss of enzymatic activity. ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01122902
更新日期:1983-04-01 00:00:00
abstract::Unstable angina pectoris (UA) is one of the most dangerous clinical symptoms of acute coronary syndrome due to the risk of myocardial ischemia, which can lead to high morbidity and mortality worldwide. Though there are many advantages in understanding the pathophysiology of UA, the identification of biomarkers for the...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181658
更新日期:2019-03-22 00:00:00
abstract::The effects of E. coli endotoxin 0127 B8 on oxygen consumption, temperature, and on the activity of the proton conductance pathway in brown adipose tissue (BAT) were investigated in rats and mice. In rats an increase was observed in rectal and skin temperature, whole body oxygen consumption and GDP binding in BAT. In ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116509
更新日期:1987-06-01 00:00:00
abstract::This paper presents a short review of our contribution to the knowledge of the structure and function of human C1r, the activation unit of C1, the first component of the classical pathway of complement. On the basis of the domain structure of C1r, a model accounting for its autolytic activation mechanism is proposed. ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01119894
更新日期:1985-10-01 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01117344
更新日期:1988-12-01 00:00:00
abstract:: Background/aims: Studies have demonstrated that osteopontin (OPN) was associated with the severity and development of knee osteoarthritis (OA). Methods: The purpose of this case-control study was to investigate the association ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20182023
更新日期:2019-02-27 00:00:00
abstract::Calpain-1 and calpain-2 are highly structurally similar isoforms of calpain. The calpains, a family of intracellular cysteine proteases, cleave their substrates at specific sites, thus modifying their properties such as function or activity. These isoforms have long been considered to function in a redundant or comple...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20200552
更新日期:2020-11-27 00:00:00