Enhancing fluid secretion by the corneal epithelium.

abstract：Purpose:Our goal was to generate and characterize a new mouse model in which only angiogenesis- and glaucoma-relevant tissues would be naturally fluorescent. The Matrix Gla (MGP) gene is highly expressed in vascular smooth muscle cells (VSMC) and trabecular meshwork (TM). We sought to direct our Mgp-Cre.KI mouse recomb...

journal_title：Investigative ophthalmology & visual science

authors： Asokan P,Mitra RN,Periasamy R,Han Z,Borrás T

更新日期：2018-02-01 00:00:00

abstract：PURPOSE:The prevalence of cataract increases with age. Conversely, the abundance of native α-crystallin diminishes with age and cataract development. We hypothesize replenishing lens α-crystallin may delay or prevent cataract. Herein we investigated the ability of cell penetration peptides (CPP) to enhance entry of α-c...

journal_title：Investigative ophthalmology & visual science

authors： Mueller NH,Ammar DA,Petrash JM

更新日期：2013-01-02 00:00:00

abstract：PURPOSE:To identify major gangliosides - the sialated glycolipids - of corneal epithelium; to determine which specific gangliosides, if any, are synthesized in a higher amount or are downregulated during corneal epithelial cell migration; and to determine what role, if any, they play in the modulation of corneal epithe...

journal_title：Investigative ophthalmology & visual science

authors： Yang Z,Zhao Z,Panjwani N

更新日期：1996-03-01 00:00:00

abstract：PURPOSE:This study was designed to investigate the suppressive effects of antithrombin (AT)III on inflammatory reactions during endotoxin-induced uveitis (EIU) in rats by studying leukocyte-endothelium interactions. METHODS:EIU was induced in Lewis rats by footpad injection of lipopolysaccharide (LPS). ATIII was admin...

journal_title：Investigative ophthalmology & visual science

authors： Yamashiro K,Kiryu J,Tsujikawa A,Honjo M,Nonaka A,Miyamoto K,Honda Y,Tanihara H,Ogura Y

更新日期：2001-06-01 00:00:00

abstract：PURPOSE:Because of its role in cell cycle regulation and apoptosis, p53 may be involved in maintaining the post-mitotic state of the adult eye. To shed light on the role of p53 in retinal development and maintenance, this study investigated the pattern of expression of p53, its family members, and its regulators during...

journal_title：Investigative ophthalmology & visual science

authors： Vuong L,Brobst DE,Saadi A,Ivanovic I,Al-Ubaidi MR

更新日期：2012-07-20 00:00:00

abstract：PURPOSE:To determine the cell-killing activity of varying doses of carboplatin, graded hyperthermia, and the combination of carboplatin and hyperthermia in the treatment of a transgenic murine retinoblastoma cell line. METHODS:Replicate cell wells (more than six wells per dose point) from an established transgenic mur...

journal_title：Investigative ophthalmology & visual science

authors： Murray TG,Cicciarelli N,McCabe CM,Ksander B,Feuer W,Schiffman J,Mieler WF,O'Brien JM

更新日期：1997-11-01 00:00:00

abstract：PURPOSE:To determine the earliest changes that occur in the retina after the onset of ectopic expression of vascular endothelial growth factor (VEGF) by photoreceptors in transgenic mice, to characterize the development of neovascularization (NV), and to determine the feasibility of using these mice to test the efficac...

journal_title：Investigative ophthalmology & visual science

authors： Tobe T,Okamoto N,Vinores MA,Derevjanik NL,Vinores SA,Zack DJ,Campochiaro PA

更新日期：1998-01-01 00:00:00

abstract：PURPOSE:The chemokine receptor CX3CR1 is expressed by monocyte-derived dendritic cells (DCs) and macrophages. CX3CR1 mediates leukocyte migration and adhesion in homeostatic and inflammatory conditions. Mice lacking Cx3cr1 have altered distribution and function of DC subpopulations in some tissue microenvironments. The...

journal_title：Investigative ophthalmology & visual science

authors： Kezic J,Xu H,Chinnery HR,Murphy CC,McMenamin PG

更新日期：2008-04-01 00:00:00

abstract：PURPOSE:Throughout adulthood, Bruch membrane (BrM) accumulates esterified cholesterol (EC) associated with abundant 60- to 80-nm-diameter lipoprotein-like particles (LLP), putative apolipoprotein B (apoB) lipoproteins secreted by the retinal pigment epithelium (RPE). In the present study, neutral lipid, phospholipids, ...

journal_title：Investigative ophthalmology & visual science

authors： Wang L,Li CM,Rudolf M,Belyaeva OV,Chung BH,Messinger JD,Kedishvili NY,Curcio CA

更新日期：2009-02-01 00:00:00

abstract：:The study and therapy of age-related macular degeneration (AMD), a leading cause of blindness worldwide, have taken great strides over the past decade. During the same time, a central role for RNA in many human diseases has been discovered. We have identified anti-angiogenic functions for synthetic double stranded RNA...

journal_title：Investigative ophthalmology & visual science

authors： Ambati J

更新日期：2011-04-06 00:00:00

abstract：PURPOSE:Homocysteine (Hcys), a well-known inducer of vascular endothelial cell damage has been associated with extracellular matrix changes. Lysyl oxidase (LOX) is a copper-dependent amine oxidase that initiates the covalent cross-linking of collagen and elastin in the extracellular matrix (ECM). LOX contributes to the...

journal_title：Investigative ophthalmology & visual science

authors： Coral K,Angayarkanni N,Gomathy N,Bharathselvi M,Pukhraj R,Rupak R

更新日期：2009-08-01 00:00:00

abstract：PURPOSE:Adeno-associated virus serotype 2 (AAV2) has been shown to be effective in transducing inner retinal neurons after intravitreal injection in several species. However, results in nonprimates may not be predictive of transduction in the human inner retina, because of differences in eye size and the specialized mo...

journal_title：Investigative ophthalmology & visual science

authors： Yin L,Greenberg K,Hunter JJ,Dalkara D,Kolstad KD,Masella BD,Wolfe R,Visel M,Stone D,Libby RT,Diloreto D Jr,Schaffer D,Flannery J,Williams DR,Merigan WH

更新日期：2011-04-25 00:00:00

abstract：:To investigate the role of cellular immunodeficiency in recurrent toxoplasmic retinochoroiditis, six Cynomolgus monkeys (Macaca fascicularis) with healed toxoplasmic lesions of the retina were immunosuppressed by total lymphoid irradiation. Three months prior to irradiation 30,000 Toxoplasma gondii organisms of the Be...

journal_title：Investigative ophthalmology & visual science

authors： Holland GN,O'Connor GR,Diaz RF,Minasi P,Wara WM

更新日期：1988-06-01 00:00:00

abstract：:Reaching a consensus in terms of interchangeability and utility (i.e., disease detection/monitoring) of a medical device is the eventual aim of repeatability and agreement studies. The aim of the tolerance and relative utility indices described in this report is to provide a methodology to compare change in clinical m...

journal_title：Investigative ophthalmology & visual science

authors： Bergin C,Guber I,Hashemi K,Majo F

更新日期：2015-08-01 00:00:00

abstract：PURPOSE:To characterize the spectrum of mutations in the OPA1 gene in a large international panel of patients with autosomal dominant optic atrophy (adOA), to improve understanding of the range of functional deficits attributable to sequence variants in this gene, and to assess any genotype-phenotype correlations. MET...

journal_title：Investigative ophthalmology & visual science

authors： Thiselton DL,Alexander C,Taanman JW,Brooks S,Rosenberg T,Eiberg H,Andreasson S,Van Regemorter N,Munier FL,Moore AT,Bhattacharya SS,Votruba M

更新日期：2002-06-01 00:00:00

abstract：:Superoxide dismutase (SOD) molecules occur in all cells exposed to an oxygen-containing environment, including retinal pigment epithelial (RPE) cells. Previous studies of nonhuman RPE have either probed specifically for copper-zinc-containing SOD (CuZn-SOD) or have not distinguished between CuZn-SOD and the SOD molecu...

journal_title：Investigative ophthalmology & visual science

authors： Newsome DA,Dobard EP,Liles MR,Oliver PD

更新日期：1990-12-01 00:00:00

abstract：PURPOSE:Point and null mutations in interphotoreceptor retinoid-binding protein (IRBP) cause retinal dystrophy in affected patients and IRBP-deficient mice with unknown mechanism. This study investigated whether IRBP protects cells from damages induced by all-trans-retinal (atRAL), which was increased in the Irbp(-/-) ...

journal_title：Investigative ophthalmology & visual science

authors： Lee M,Li S,Sato K,Jin M

更新日期：2016-04-01 00:00:00

abstract：PURPOSE:There is considerable variation in the degree of form-deprivation myopia (FDM) induced in chickens by a uniform treatment regimen. Sex and pretreatment eye size have been found to be predictive of the rate of FD-induced eye growth. Therefore, this study was undertaken to test whether the greater rate of myopic ...

journal_title：Investigative ophthalmology & visual science

authors： Chen YP,Prashar A,Hocking PM,Erichsen JT,To CH,Schaeffel F,Guggenheim JA

更新日期：2010-02-01 00:00:00

abstract：PURPOSE:To assess dynamic interactions of eye and head movements during return-sweep saccades (RSS) when reading with single-vision (SVL) versus progressive-addition (PAL) lenses in a simulated computer-based business environment. METHODS:Horizontal eye and head movements were recorded objectively and simultaneously a...

journal_title：Investigative ophthalmology & visual science

authors： Han Y,Ciuffreda KJ,Selenow A,Ali SR

更新日期：2003-04-01 00:00:00

abstract：:Parallel studies of the ionic balance and membrane permeability characteristics of normal human lenses were carried out in three countries (USA, England and Italy). Similar age-related changes were found in each laboratory. The lens membrane potential and resistance declined markedly with age while internal Na+ and fr...

journal_title：Investigative ophthalmology & visual science

authors： Duncan G,Hightower KR,Gandolfi SA,Tomlinson J,Maraini G

更新日期：1989-08-01 00:00:00

abstract：:The comparison of intracellular pH (pHi) regulation between fresh (FBCE) and cultured (CBCE) bovine corneal endothelium was extended to HCO3- transport mechanisms. Upon introduction of CO2/HCO3- Ringer's solution, there was a small, sharp acidification followed by an alkalinization .09 and .18 pH U above the HCO3- fre...

journal_title：Investigative ophthalmology & visual science

authors： Bonanno JA,Giasson C

更新日期：1992-10-01 00:00:00

abstract：PURPOSE:Currently, there are no definitive immunomarkers for epithelial stem cells (corneal and conjunctival) or their poorly understood niche microenvironment. The H2B-GFP/K5tTA mouse enables visualization of label-retaining cells (LRCs), which exhibit the functional marker of stem cell quiescence. We used immunofluor...

journal_title：Investigative ophthalmology & visual science

authors： Parfitt GJ,Kavianpour B,Wu KL,Xie Y,Brown DJ,Jester JV

更新日期：2015-11-01 00:00:00

abstract：PURPOSE:Mice rendered hypoglycemic by a null mutation in the glucagon receptor gene Gcgr display late-onset retinal degeneration and loss of retinal sensitivity. Acute hyperglycemia induced by dextrose ingestion does not restore their retinal function, which is consistent with irreversible loss of vision. The goal of t...

journal_title：Investigative ophthalmology & visual science

authors： Umino Y,Cuenca N,Everhart D,Fernandez-Sanchez L,Barlow RB,Solessio E

更新日期：2012-02-21 00:00:00

abstract：PURPOSE:To identify if whole-body hydration plays an important role in dry eye (DE). We hypothesized that individuals classified as DE have higher plasma osmolality (Posm), indicating suboptimal hydration, compared with those classified as non-DE. METHODS:Using a hospital-based observational cross-sectional design, as...

journal_title：Investigative ophthalmology & visual science

authors： Walsh NP,Fortes MB,Raymond-Barker P,Bishop C,Owen J,Tye E,Esmaeelpour M,Purslow C,Elghenzai S

更新日期：2012-09-25 00:00:00

abstract：:An objective visual field can be mapped in terms of stimulus-induced eye movement. The authors used the scleral coil technique to record vergence and conjugate eye movements while stimulating different visual field locations with a 3 X 3 deg target whose image vergence was oscillated. For each of three subjects tested...

journal_title：Investigative ophthalmology & visual science

authors： Regan D,Erkelens CJ,Collewijn H

更新日期：1986-05-01 00:00:00

abstract：PURPOSE:To determine if cataract surgery on eyes with AMD confers as much functional visual improvement as surgery on eyes without retinal pathology. METHODS:This is a retrospective analysis of 4924 cataract surgeries from the Veterans Healthcare Administration Ophthalmic Surgical Outcomes Data Project (OSOD). We incl...

journal_title：Investigative ophthalmology & visual science

authors： Stock MV,Vollman DE,Baze EF,Chomsky AS,Daly MK,Lawrence MG

更新日期：2015-04-01 00:00:00

abstract：PURPOSE:To examine the prognostic relevance of expression of the chemokine receptors CCR7 and CXCR4 and its ligand CXCL12 in uveal melanoma in nonmetastatic and metastatic patients with correlation to liver metastasis and overall survival. METHODS:Primary uveal melanoma specimens from 19 patients with correlating live...

journal_title：Investigative ophthalmology & visual science

authors： van den Bosch T,Koopmans AE,Vaarwater J,van den Berg M,de Klein A,Verdijk RM

更新日期：2013-11-08 00:00:00

abstract：Purpose:To quantify the effect of induced stray light on halo size, luminance threshold, and contrast sensitivity. Methods:Retinal stray light was induced in five healthy subjects using different photographic filters. The stray light induced ranged from levels observed in intraocular lenses (IOLs) with glistenings (lo...

journal_title：Investigative ophthalmology & visual science

authors： van der Mooren M,Rosén R,Franssen L,Lundström L,Piers P

更新日期：2016-10-01 00:00:00

abstract：PURPOSE:The incidence of blindness is increasing, in part, because of abnormal ocular neovascularization. Anti-VEGF therapies have yielded impressive results; however, they are not a cure for blindness. Recently, metallothioneins (MTs) 1 and 2 have been implicated in the process of angiogenesis. Therefore, we investiga...

journal_title：Investigative ophthalmology & visual science

authors： Nakamura S,Shimazawa M,Inoue Y,Takata S,Ito Y,Tsuruma K,Ikeda T,Honda A,Satoh M,Hara H

更新日期：2014-09-23 00:00:00

abstract：PURPOSE:To develop and characterize a feline model of retinal degeneration induced by intravitreal injection of adenosine triphosphate (ATP). METHODS:Nineteen normally sighted adult cats received 100 μL intravitreal injections of ATP with a final concentration of 11, 22, or 55 mM at the retina. Four animals were eutha...

journal_title：Investigative ophthalmology & visual science

authors： Aplin FP,Luu CD,Vessey KA,Guymer RH,Shepherd RK,Fletcher EL

更新日期：2014-11-25 00:00:00