Abstract：
PURPOSE:Currently, there are no definitive immunomarkers for epithelial stem cells (corneal and conjunctival) or their poorly understood niche microenvironment. The H2B-GFP/K5tTA mouse enables visualization of label-retaining cells (LRCs), which exhibit the functional marker of stem cell quiescence. We used immunofluorescence tomography to evaluate putative stem cell markers and LRCs of the mouse ocular surface. METHODS:H2B-GFP/K5tTA mice were pulsed for 56 days and then chased with doxycycline to label LRCs. Limbus and eyelid tissue was 3-dimensionally (3-D) reconstructed using immunofluorescence tomography to identify and characterize LRCs using the putative stem cell markers sox9, keratin 19, lrig1, blimp1, and abcb5. RESULTS:After 28 days of chase, LRCs were localized to the entire limbus epithelium and, infrequently, the anterior limbal stroma. Label-retaining cells comprised 3% of limbal epithelial cells after 56 days of chase. Conjunctival LRCs were localized to the fornix and comprised 4% of the total fornix epithelial cells. No stem cell immunomarker was specific for ocular surface LRCs; however, blimp1 enriched for limbal basal epithelial cells and 100% of green fluorescent protein-positive (GFP+) cells at the limbus and fornix were found to be lrig1-positive. CONCLUSIONS:Label-retaining cells represent a larger population of the mouse limbus than previously thought. They decrease in number with increased doxycycline chase, suggesting that LRC populations with different cell cycle lengths exist at the limbus. We conclude that current immunomarkers are unable to colocalize with the functional marker of epithelial stem cell quiescence; however, blimp1 may enrich for limbal epithelial basal cells.
journal_name
Invest Ophthalmol Vis Scijournal_title
Investigative ophthalmology & visual scienceauthors
Parfitt GJ,Kavianpour B,Wu KL,Xie Y,Brown DJ,Jester JVdoi
10.1167/iovs.15-18038subject
Has Abstractpub_date
2015-11-01 00:00:00pages
7338-44issue
12eissn
0146-0404issn
1552-5783pii
2470402journal_volume
56pub_type
杂志文章abstract：PURPOSE:Invasion of tumor cells into blood vessels is essential for metastasis of uveal melanoma. The occurrence of ingrowth of tumor cells in blood vessels in uveal melanoma was analyzed, and this parameter was compared with the survival of the patients. METHODS:Between 1972 and 2007, 643 eyes primarily enucleated fo...
journal_title：Investigative ophthalmology & visual science
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abstract：PURPOSE:To determine the effects of enzymes on mammalian vitreous gel and to thus infer the structural roles of hyaluronan and chondroitin sulfate in the gel. METHODS:The wet weights of bovine vitreous gels were compared before and after incubation with Streptomyces hyaluronan lyase, chondroitin ABC lyase, testicular ...
journal_title：Investigative ophthalmology & visual science
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doi：
更新日期：1999-09-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
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doi：10.1167/iovs.06-0301
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abstract：PURPOSE:Elevated levels of extracellular glutamate have been implicated in the pathophysiology of neuronal loss in both central nervous system and ophthalmic disorders, including glaucoma. This increase in glutamate may result from a failure of glutamate transporters (molecules that ordinarily regulate extracellular gl...
journal_title：Investigative ophthalmology & visual science
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abstract：PURPOSE:Endocannabinoids are important modulators of synaptic transmission and plasticity throughout the central nervous system. The cannabinoid receptor type 1 (CB1R) is extensively expressed in the adult retina of rodents, while CB2R mRNA and protein expression have been only recently demonstrated in retinal tissue. ...
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abstract：Purpose:To determine if cytokine tear levels before hematopoietic stem cell transplantation (HSCT) can help anticipate the occurrence of ocular chronic graft-versus-host disease (cGVHD). Methods:In this pilot study, 25 patients undergoing HSCT were followed prospectively for ≤43 months. After ocular examinations, tear...
journal_title：Investigative ophthalmology & visual science
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abstract：Purpose:During development, the corneal epithelium (CE) and the conjunctiva are derived from the surface ectoderm. Here we have examined how, during development, the cells of these two issues become isolated from each other. Methods:Epithelia from the anterior eyes of chicken embryos were labeled with the fluorescent,...
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journal_title：Investigative ophthalmology & visual science
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journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.05-0234
更新日期：2006-01-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
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doi：
更新日期：1985-08-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
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abstract：:We examined the sources of artifacts of vitreous fluorophotometry (VF), a technique used to assess the permeability of the blood-retinal barrier. By performing in vitro measurements in a model eye and in vivo readings in humans, we investigated the influence of various concentrations of fluorescein in the anterior cha...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1982-05-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.13-12650
更新日期：2013-11-21 00:00:00
abstract：PURPOSE:To test the effect of valacyclovir alone and with aspirin on the asymptomatic shedding of HSV-1 DNA in tears and saliva of healthy individuals. METHOD. The subjects (n = 45) were randomized into three groups without regard to age, sex, or race. Group 1 (n = 14) received the placebo, group 2 (n = 15) received a ...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章,随机对照试验
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更新日期：2009-12-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
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abstract：:Topical application of Prostaglandin F2 alpha (PGF2 alpha) to the eye reduces intraocular pressure (IOP) in all mammalian species studied thus far, including humans. The L-isopropylester derivative is currently the one most commonly used in experimental and clinical studies. Dose-response relationships were determined...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1991-03-01 00:00:00
abstract：PURPOSE:To investigate the choroidal thickness (CT) in children with amblyopia through spectral-domain optical coherence tomography (SD-OCT). METHODS:Thirty-seven children with unilateral amblyopia and 22 children with normal vision participated in the study. Cross-sectional images of the choroid of evaluated eyes wer...
journal_title：Investigative ophthalmology & visual science
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更新日期：2014-10-14 00:00:00
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journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章,多中心研究,随机对照试验
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journal_title：Investigative ophthalmology & visual science
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doi：
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journal_title：Investigative ophthalmology & visual science
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doi：
更新日期：2001-08-01 00:00:00
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更新日期：2004-09-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
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doi：
更新日期：1994-07-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
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doi：
更新日期：1986-12-01 00:00:00
abstract：PURPOSE:Classic studies have demonstrated that corneal epithelial cell density in culture can alter the balance of stimulatory and inhibitory cytokines controlling the elaboration of collagenolytic activity by co-cultured stromal cells. The current study attempts to bring the understanding of this mechanism to a molecu...
journal_title：Investigative ophthalmology & visual science
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doi：
更新日期：1997-02-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
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更新日期：2002-03-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
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更新日期：1986-01-01 00:00:00
abstract：PURPOSE:Neuronal and glial alterations precede the overt vascular change that characterizes diabetic retinopathy. Because retinal astrocytes modulate neuronal and vascular function, this study investigated the time course of astrocyte, Müller cell, and neuronal change during diabetes to determine whether astrocytes may...
journal_title：Investigative ophthalmology & visual science
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