Abstract:
:The EMS1 gene encodes an 80/85 kDa c-src substrate and localises with the CCND1 gene to chromosome 11q13. This locus is amplified in approximately 13% of human breast cancers. EMS1 gene amplification and expression were characterised in a panel of human breast cancer cell lines to determine at what levels expression is regulated. The degree of tyrosine phosphorylation of EMS1 protein was also determined and compared with the activity of src-family kinases. The EMS1 gene was amplified in 6 of 20 cell lines investigated: MDA-MB-134, -157, -175, -453, ZR-75-1 and MCF-7. In the MDA-MB-157 and MCF-7 cell lines, EMS1 was amplified in the absence of CCND1 gene amplification. EMS1 protein levels were increased relative to normal breast epithelial cells in 6 cell lines (ZR-75-1, MDA-MB-134, -175, 453, MCF-7 and BT-474). Of these, BT-474 is the only cell line that does not exhibit EMS1 amplification or increased EMS1 mRNA levels. EMS1 tyrosine phosphorylation was 3-fold higher in BT-474 and T-47D cells, which exhibited relatively high total src activity coupled with expression of both c-fyn and c-yes, than in MDA-MB-453 cells, which expressed only c-yes. Our results therefore demonstrate gene amplification to be the predominant mechanism underlying EMS1 over-expression in human breast cancer cell lines and identify tyrosine phosphorylation as a further level at which regulation of this protein may be perturbed.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Campbell DH,deFazio A,Sutherland RL,Daly RJdoi
10.1002/(SICI)1097-0215(19961115)68:4<485::AID-IJCsubject
Has Abstractpub_date
1996-11-15 00:00:00pages
485-92issue
4eissn
0020-7136issn
1097-0215pii
10.1002/(SICI)1097-0215(19961115)68:4<485::AID-IJCjournal_volume
68pub_type
杂志文章abstract::During the course of a mechanism-based screening program aimed at identifying new antimitotic agents, a novel microtubule depolymerizing piperazine derivative, 1-(5-chloro-2-methoxybenzoyl)-4-(3-chlorophenyl) piperazine, was identified. The compound, designated CB694, caused inhibition of proliferation of a wide range...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21424
更新日期:2006-02-15 00:00:00
abstract::Sexually transmitted genital human papillomavirus (HPV) infection, most often HPV 16, is considered the major etiologic determinant of cervical cancer. However, some studies have found relatively low prevalences of genital tract HPV DNA in some geographical areas, such as Greenland, that have high rates of cervical ca...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(19961211)68:6<704::AID-IJC
更新日期:1996-12-11 00:00:00
abstract::Chimeric bcr/abl fusion proteins are thought to be the molecular 'pathogen' of chronic myelogenous leukaemia (CML). Expression levels of the respective fusion RNAs reflect disease progression as well as remission upon therapeutic intervention in CML patients. However, there is no quick and reliable method that would a...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(20000601)86:5<741::aid-ijc
更新日期:2000-06-01 00:00:00
abstract::The population of Leningrad suffered from severe starvation, cold and psychological stress during the siege in World War II in 1941-1944. We investigated the long-term effects of the siege on cancer mortality in 3,901 men and 1,429 women, born between 1910 and 1940. All study subjects were residents of St. Petersburg,...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24093
更新日期:2009-03-15 00:00:00
abstract::Allelic losses on the chromosome arms 1p and 19q have been associated with favorable response to chemotherapy and good prognosis in anaplastic oligodendroglioma patients, but the molecular mechanisms responsible for this relationship are as yet unknown. The DNA repair enzyme O(6)-methylguanine DNA methyltransferase (M...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.20575
更新日期:2005-01-20 00:00:00
abstract::The promoter variants of TNF-α, a major regulator of immune and inflammation responses, have been implicated in cancer development and prognosis. Thus, we investigated associations between four TNF-α promoter variants and risk of recurrence of squamous cell carcinoma of the oropharynx (SCCOP). We evaluated association...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.28512
更新日期:2014-04-15 00:00:00
abstract::MicroRNAs (mirs) are small noncoding RNA molecules (~22 nucleotides) that regulate posttranscriptional gene expression. Currently, there has not been a comprehensive study of their role in primary head and neck squamous cell carcinoma (HNSCC). To determine the role of mirs in HNSCC, we screened for altered microRNA ex...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23831
更新日期:2008-12-15 00:00:00
abstract::Breast cancers diagnosed between screening examinations among women who attend a breast cancer screening program are defined as interval cancers. The Norwegian Breast Cancer Screening Program started as a pilot project in 1996, and data from the first 2-year interval are available. Our study quantifies interval cancer...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.1511
更新日期:2001-11-01 00:00:00
abstract::In order to examine whether or not the expression of the apoptosis-related receptor Fas (CD-95/APO-1) and its ligand (FasL) has relevance for patient survival, immunohistochemistry was used to analyze the proteins of both factors in 164 non-small cell lung carcinomas. Patients with Fas-positive tumors exhibited signif...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19990621)84:3<239::aid-ijc
更新日期:1999-06-21 00:00:00
abstract::Our study demonstrates that synthetic sialyl Lewis X (SLex) as a ligand for selectins and fibronectin-derived RGDS peptide analogue [Ar(DRGDS)3] inhibits lung metastases produced by i.v. co-injection of B16-BL6 melanoma cells. To investigate the inhibitory mechanisms in a living animal, we performed positron-emission ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(19960315)65:6<833::AID-IJC
更新日期:1996-03-15 00:00:00
abstract::CD4(+) T cells have been shown to be crucial for the induction and maintenance of cytotoxic T cell responses and to be also capable of mediating direct tumor rejection. Therefore, the anticancer therapeutic efficacy of peptide-based vaccines may be improved by addition of HLA class II epitopes to stimulate T helper c...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.26365
更新日期:2012-07-01 00:00:00
abstract::We investigated whether variants in sex steroid hormone metabolism genes modify the effect of hormone therapy (HT) on endometrial cancer risk in postmenopausal non-Hispanic white women. A nested case-control study was conducted within the California Teachers Study (CTS). We genotyped htSNPs in six genes involved in th...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.26163
更新日期:2012-04-01 00:00:00
abstract::Recently, several studies have showed that FAS (rs2234767, rs1800682) and FASL (rs763110) functional single nucleotide polymorphisms (SNPs) were associated with the risk of various cancers. However, the association between cervical cancer risk and the three SNPs above remained inconclusive. In this work, we performed ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.30490
更新日期:2017-02-15 00:00:00
abstract::There are approximately 100 genes which when mutated are known to predispose to one or more forms of cancer. Currently, genetic testing is offered for many of these, either as single genes or as multi-gene panels. Features of hereditary cancer include a positive family history of cancer, early age of onset and the app...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
doi:10.1002/ijc.28988
更新日期:2014-10-15 00:00:00
abstract::A pilot case-control study on bladder cancer, with population-based controls matched for each of 74 cases, has been conducted in two industrial areas of Southern Belgium in order to analyze the influence of tobacco use and occupation. Observed bladder cancer risk for smokers is more than 5 times higher than that for n...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910390304
更新日期:1987-03-15 00:00:00
abstract::Oncolytic adenoviruses are being developed as novel anticancer therapeutics and currently undergoing clinical trials. We previously demonstrated that telomerase-specific replication-competent adenovirus (Telomelysin: OBP-301), in which the human telomerase reverse transcriptase (hTERT) promoter regulates viral replica...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21846
更新日期:2006-07-15 00:00:00
abstract::The physiological stress response to surgery promotes wound healing and functional recovery and includes the activation of neural, inflammatory and proangiogenic signaling pathways. Paradoxically, the same pathways also promote metastatic spread and growth of residual cancer. Human and animal studies show that cancer ...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
doi:10.1002/ijc.32820
更新日期:2020-06-15 00:00:00
abstract::We report the relative frequency of sucrase-isomaltase (SI) antigen expression in human colonic adenocarcinoma (22/57), in peritumoral mucosa taken next to the tumor (31/41) or distant from it (29/42) as well as in 21/23 polyps. Our results are based on indirect immunofluorescence with a monoclonal antibody (MAb) spec...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910440209
更新日期:1989-08-15 00:00:00
abstract::Interaction between CD40 and its ligand, CD154, has a key function in immune regulation. Recent experimental data support a role of deregulated CD40 signalling in lymphomagenesis. Data from earlier studies that are part of this pooling study implicate a functional polymorphism (-1C>T, rs1883832) in the TNFRSF5 gene en...
journal_title:International journal of cancer
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1002/ijc.25420
更新日期:2011-03-15 00:00:00
abstract::Current evidence suggests poor identification and referral of Lynch syndrome patients. This study evaluated the strategies by which patients with endometrial cancer were referred to genetics services. Data from clinic-based patients with endometrial cancer enrolled through the Australian National Endometrial Cancer po...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.27978
更新日期:2013-06-15 00:00:00
abstract::The age-adjusted lung cancer incidence rates among Japanese, Chinese and Hawaiian women in Hawaii do not correlate to their respective cigarette smoking exposures. We conducted a case-control study to determine the lung cancer risk associated with smoking among these three ethnic groups of women by reviewing the medic...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910270307
更新日期:1981-03-15 00:00:00
abstract::A major objective of peptide vaccination is the induction of tumor-reactive CD8+ T-cells. We have shown that HLA-A2 positive cancer patients frequently develop an antigen-specific CD8+ T-cell response after vaccination with NY-ESO-1 peptides p157-165/p157-167. These T-cells are highly reactive with the peptides used f...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.22957
更新日期:2007-11-01 00:00:00
abstract::Mammalian LIM kinase 1 (LIMK1) phosphorylates and inactivates the actin-binding and -depolymerizing factor cofilin and induces actin cytoskeletal changes. LIMK1 is reported to play an important role in cell motility, but the mechanism of induction of cell motility and the role of LIMK1 in tumor growth, angiogenesis an...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21650
更新日期:2006-06-01 00:00:00
abstract::Chemokine receptors are known to regulate homing of lymphocytes into secondary lymphoid organs and may also be involved in the lymphatic spread of solid tumors. Therefore, the assessment of chemokine receptor expression on colorectal carcinomas could potentially improve the prediction of lymph node spread. This is of ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21123
更新日期:2005-09-20 00:00:00
abstract::Epigenetic silencing of MLH1 is the most common cause of defective DNA mismatch repair in endometrial and colorectal cancers. We hypothesized that variation in the MLH1 gene might contribute to the risk for MLH1 methylation and epigenetic silencing. We undertook a case-control study to test for the association between...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.22406
更新日期:2007-04-15 00:00:00
abstract::The cytokines that regulate angiogenesis in normal and malignant prostate tissue are not well studied. Using an RT-PCR-based screen, we observed that cultured, low-passage normal human prostate epithelial cells (PrECs) express a variety of cytokines which have been shown to have angiogenic and/or endothelial cell-acti...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19990315)80:6<868::aid-ijc
更新日期:1999-03-15 00:00:00
abstract::The absolute risk reduction by prophylaxis in chemotherapy-induced febrile neutropenia (FN) is largest in patients at highest underlying risk. Therefore, reliable predictive models are needed. Here, we develop and validate such a model for risk of FN during chemotherapy cycles 2-6. A prediction score for risk of FN du...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32249
更新日期:2020-01-15 00:00:00
abstract::Murine mammary aplastic carcinoma, when it is passed from one alloxan-diabetic animal to another, after several passages becomes conditioned to hypoinsulinemia by secreting its own substance(s) immunologically cross-reactive with insulin (SICRI). Although not cytotoxic, the total daily dose of 2.5 mg of synthetic line...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910290614
更新日期:1982-06-15 00:00:00
abstract::There are no reliable serologic tumor markers for transitional cell carcinoma (TCC) of the urinary bladder and noninvasive urine investigations are inadequate. We used fluorescent microsatellite analysis (MSA) to detect serum DNA and urine-sediment DNA alterations in patients with bladder cancer and prospectively coll...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.1434
更新日期:2001-10-01 00:00:00
abstract::A novel non-phorbol-ester-like tumor promoter, okadaic acid (OA) has been shown to be an inhibitor of protein phosphatase I and IIA and, thus, to cause an "apparent activation" of protein kinase C (PKC). We previously showed that cis-diamminedichloroplatinum(II) (CDDP)-resistant cells, PC-9/CDDP, were cross-resistant ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910500319
更新日期:1992-02-01 00:00:00