Abstract:
:The cytokines that regulate angiogenesis in normal and malignant prostate tissue are not well studied. Using an RT-PCR-based screen, we observed that cultured, low-passage normal human prostate epithelial cells (PrECs) express a variety of cytokines which have been shown to have angiogenic and/or endothelial cell-activating properties in various systems. These include vascular endothelial growth factor (VEGF), basic fibroblastic growth factor (bFGF), transforming growth factor-alpha (TGF-alpha), transforming growth factor-beta (TGF-beta), interleukin-8 (IL-8), tumor necrosis factor-alpha (TNF-alpha), granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF). Expression of VEGF, bFGF, GM-CSF, G-CSF, TGF-alpha and TNF-alpha in these cells was confirmed by immunohistochemistry. Culture medium conditioned by normal human PrECs for periods of up to 96 hr were found to contain VEGF, GM-CSF, G-CSF, IL-8, TGF-beta1 and TGF-beta2 but not TNF-alpha or bFGF, as determined by ELISA. Of these, VEGF was by far the most prominently expressed angiogenic cytokine (approx. 2,500 pg/ml conditioned medium at 96 hr vs. 30 to 100 pg/ml conditioned medium for the other cytokines). PrEC-conditioned medium induced an approximately 2-fold stimulation of [3H]-thymidine incorporation in cultured human umbilical cord endothelial cells (HUVECs) deprived of the endothelial growth factors VEGF and bFGF; this stimulation was abolished by neutralizing antibodies directed against VEGF but not bFGF, IL-8, GM-CSF or TNF-alpha. VEGF expression by PrECs was not markedly altered by administration or deprivation of other angiogenic cytokines for which these cells have receptors, suggesting that there is not a hierarchy of cytokines controlling its expression; however, retinoic acid, a component of PrEC growth medium, was found to modestly suppress VEGF at physiological concentrations (0.1 ng/ml). These data suggest that normal PrECs express a variety of angiogenic cytokines, most prominently VEGF, to recruit a supporting vasculature, even in culture. Our data also suggest that the ability of malignant PrECs to stimulate angiogenesis may be intrinsic and does not need to be acquired during oncogenesis.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Campbell CL,Savarese DM,Quesenberry PJ,Savarese TMdoi
10.1002/(sici)1097-0215(19990315)80:6<868::aid-ijckeywords:
subject
Has Abstractpub_date
1999-03-15 00:00:00pages
868-74issue
6eissn
0020-7136issn
1097-0215pii
10.1002/(SICI)1097-0215(19990315)80:6<868::AID-IJCjournal_volume
80pub_type
杂志文章abstract::After a steady increase between the 1950s and the 1970s, laryngeal cancer mortality has been levelling off since the early 1980s in men from most western and southern European countries and since the early 1990s in central and eastern Europe. To update trends in laryngeal cancer mortality, we analyzed data provided by...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29833
更新日期:2016-02-15 00:00:00
abstract::Using a recently developed autochthonous mouse model of squamous cell carcinoma (SCC), a combination therapy targeting polyamine metabolism was evaluated. The therapy combined 2-difluoromethylornithine (DFMO), an inhibitor of ornithine decarboxylase (ODC), and MQT 1426, a polyamine transport inhibitor. In 1 trial last...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21621
更新日期:2006-05-01 00:00:00
abstract::Tumour necrosis factor alpha (TNF) mRNA is detected in the macrophage infiltrate surrounding the tumour, but the cellular/molecular interactions leading to TNF gene expression in macrophages are unknown. The in vitro system in which human blood monocytes are stimulated with human cancer cells for TNF release was used ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910560221
更新日期:1994-01-15 00:00:00
abstract::The current paradigm stipulates that inhibition of histone deacetylase (HDAC) 6 is essential for the combinatorial effect of proteasome and HDAC inhibitors for the treatment of cancers. Our study aims to investigate the effect of combining different class I HDAC inhibitors (without HDAC6 action) with a proteasome inhi...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.28924
更新日期:2014-12-15 00:00:00
abstract::Using morphometric and immunohistological techniques, we investigated the distribution of T lymphocytes and subsets, B lymphocytes, macrophages and plasma cells in sections of human breast tissue. In normal lobules and ducts, leukocytes were found in greater density in the epithelium than in the stroma. The intra-epit...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910360404
更新日期:1985-10-15 00:00:00
abstract::The high recurrence rate of bladder cancer is probably due to an efficient repopulation of the bladder by residual transformed cells after resection of the tumour. However, the regenerating capacity of the normal urothelial cells is very high. To study the balance between regenerating normal urothelium and out-growth ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910600523
更新日期:1995-03-03 00:00:00
abstract::Activated T cells expressing endogenous or transduced TCRs are two cell types currently used in clinical adoptive T-cell therapy. The ability of these cells to recognize their antigen, expand and traffic to the tumor site are the initial steps necessary for successful therapy. In this study, we used in vivo biolumines...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29401
更新日期:2015-07-15 00:00:00
abstract::Papillary thyroid carcinoma (PTC) is the most common type of thyroid cancer. Tumor recurrence occurs in ∼20% of PTCs and some reach advanced stages. Promoter mutation in the telomerase reverse transcriptase (TERT) gene is identified to be a prognostic marker in PTC. However, the contribution of TERT promoter mutation ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31225
更新日期:2018-05-15 00:00:00
abstract::Seventy to 40% of K-RAS wild type colorectal tumors does not seem to benefit from treatment with antiepidermal growth factor receptor (anti-EGFR) monoclonal antibodies. Recent data suggested that in presence of IGF-1 system, altered activation colorectal cancer cells may escape anti-EGFR mediated cell death. The inter...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25193
更新日期:2010-10-15 00:00:00
abstract::Although early detection and systemic therapies have improved the diagnosis and clinical cure rate of breast cancer, breast cancer remains the most frequently occurring malignant cancer in women due to a lack of sufficiently effective treatments. Thus, to develop potential targeted therapies and thus benefit more pati...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32922
更新日期:2020-07-15 00:00:00
abstract::The expression of a gastrointestinal carcinoma-associated antigen (GICA), a monosialoganglioside, was investigated in tissues from human fetuses of various gestational ages (10-40 weeks). Mouse monoclonal antibody NS-19-9, generated in mice immunized with SW1116 human colon carcinoma cells, was used along with a secon...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910340208
更新日期:1984-08-15 00:00:00
abstract::An important goal in prostate cancer research is to define specific molecular and cellular alterations that are associated with malignant progression. The mouse prostate reconstitution model is a relevant and useful system as it allows the study of early events in cancer progression under conditions where oncogene-ini...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910600321
更新日期:1995-01-27 00:00:00
abstract::CD22 antibodies (Abs) bound to B-cell lymphomas are known to be internalized and catabolized rapidly. Therefore, it would be expected that use of CD22 as a target for radioimmunotherapy should be enhanced by the use of "residualizing" radiolabels, which are trapped within the cell after catabolism of the Ab to which t...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19970502)71:3<429::aid-ijc
更新日期:1997-05-02 00:00:00
abstract::Statins are widely used for the treatment of hypercholesterolemia. However, their inhibitory action on HMG-CoA reductase also results in the depletion of intermediate biosynthetic products, which importantly contribute to cell proliferation. The aim of the present study was to compare the effects of the individual com...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23242
更新日期:2008-03-15 00:00:00
abstract::Daphnane-type diterpene gnidimacrin isolated from the Chinese plant Stellera chamaejasme L. is an antitumor agent that activates protein kinase C (PKC). The mechanism of antitumor action of gnidimacrin and the possible involvement of PKC were examined using sensitive K562 and refractory HLE cells. Gnidimacrin did bind...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19980717)77:2<243::aid-ijc
更新日期:1998-07-17 00:00:00
abstract::cis-Diamminedichloroplatinum(II) (CDDP) induced G2-phase arrest in PC-9 human cancer cells. To elucidate how CDDP acts on cell-cycle regulation, we analyzed the effect of CDDP on cell-cycle regulators such as p34cdc2 protein kinase. p34cdc2 protein kinase activity was maximum in G2 phase and decreased after G2/M trans...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910550417
更新日期:1993-10-21 00:00:00
abstract::C57BL/6J mice immunized with devitalized Ehrlich tumor (ET) cells produce high serum levels of IgM antibodies to ET cell-surface carbohydrates that are critical in the observed resistance against this tumor. However, this response is not found in ET-bearing mice at any stage of tumor development. Since previous studie...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910470116
更新日期:1991-01-02 00:00:00
abstract::The E2F family of transcription factors plays a key role in the control of cell-cycle progression. Some family members may act as oncogenes, others as tumor-suppressor genes. The genetic changes and the expression of E2F-1 and -3 were examined in human gastric and colorectal carcinomas by Southern, Northern and Wester...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19990517)81:4<535::aid-ijc
更新日期:1999-05-17 00:00:00
abstract::In a retrospective analysis of 203 patients with small cell lung cancer (SCLC), we examined the prognostic value of c-kit expression on survival. Expression of c-kit was examined immunohistochemically in formalin-fixed, paraffin-embedded tissue sections. c-kit was observed in 87.7% of SCLC tumors. Using the Kaplan-Mei...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.20252
更新日期:2004-08-20 00:00:00
abstract::Primary mediastinal B-cell lymphoma is a locally highly aggressive but poorly disseminating tumor composed of medium sized or large cells most probably of thymic medullary origin. It has a mature B-cell phenotype, typically lacks immunoglobulin expression and has variable defects in expression of HLA-molecules. We pre...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.1211
更新日期:2001-05-01 00:00:00
abstract::Data are limited regarding cancer risk in human immunodeficiency virus (HIV)-infected persons with modest immunosuppression, before the onset of acquired immunodeficiency syndrome (AIDS). For some cancers, risk may be affected by highly active antiretroviral therapy (HAART) widely available since 1996. We linked HIV/A...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23487
更新日期:2008-07-01 00:00:00
abstract::Chemotherapy is a well-established risk factor for acute myeloid leukemia (AML) but little is known about other prescription drugs and AML risk. We report data from a population-based Los Angeles County study in which 299 matched case-control pairs had complete data on prescription drug use and 88% of cases were subty...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.20788
更新日期:2005-04-20 00:00:00
abstract::Benzo(a)pyrene and benz(a) anthrancene which, in contrast to the K-region epoxides benzo(a)pyrene 4,5-oxide and benz(a)anthracene 5,6-oxide, are not mutagenic to Salmonella typhimurium TA 1537 in the absence of mammalian enzyme preparations, were activated by liver microsomes from C3H mice, which had not received any ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910180408
更新日期:1976-10-15 00:00:00
abstract::Obesity is a well-known risk factor for postmenopausal breast cancer. In contrast, the relationship between obesity and stage of breast cancer at diagnosis is less clear. We hypothesized that increased breast size in obese women may delay discovery of breast tumors. Thus, the purpose of our study was to examine whethe...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10209
更新日期:2002-03-10 00:00:00
abstract::Colorectal cancer is the third most common form of malignancy, behind prostate and lung cancers. Despite recent advances in medicine, mortality from colorectal cancer remains high, highlighting the need for improved therapies. Numerous studies have demonstrated increased activation of EGFR and its family members (EGFR...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25410
更新日期:2011-02-15 00:00:00
abstract::Modulation of cytoplasmic Ca++ concentration is a mechanism common to signal transduction pathways regulating many cellular phenomena, including the interactions of tumors with the hemostatic system. We have investigated the pro-aggregating and pro-coagulant activities of human tumor cell lines cultured in vitro and t...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910620310
更新日期:1995-07-28 00:00:00
abstract::The role of different types of alcoholic beverage on cancer of the upper digestive tract was investigated in a case-control study conducted in the northern part of Italy on 305 male cases of cancer of the oral cavity and pharynx, 288 of oesophageal cancer and 1621 controls admitted to hospital for acute, non-neoplasti...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910460612
更新日期:1990-12-15 00:00:00
abstract::In experimental tumor immunotherapy, incomplete Freund's adjuvant (IFA) has been considered as the "gold standard" for T-cell vaccination in mice and humans in spite of its considerable adverse effects. Recently, we succeeded in eliciting strong CTL responses in mice after vaccination with biodegradable poly(D,L-lacti...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25914
更新日期:2011-07-15 00:00:00
abstract::Mucinous colorectal cancer often presents at an advanced stage. We have previously observed that mucin production by human colon-cancer cells correlates with their ability to colonize the liver in experimental animal models. The present study was undertaken in order to further elucidate the mechanisms by which product...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910520113
更新日期:1992-08-19 00:00:00
abstract::Epidermal growth factor receptor (EGFR) plays an important role in essential cellular processes such as proliferation, survival and migration. Aberrant activation of EGFR is frequently found in human cancers of various origins and has been implicated in cancer pathogenesis. The therapeutic antibody cetuximab (Erbitux)...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.28585
更新日期:2014-06-01 00:00:00