Abstract:
:Calf thymus DNA was reacted with 1,2-epoxybutene (BDO) in phosphate buffered saline at 37 degrees C for 12 h. DNA was ethanol precipitated and hydrolyzed with 1 M HCl to release DNA bases. Adenine (A)-BDO and guanine (Gua)-BDO adducts were analyzed in the ethanol supernatant and DNA hydrolysate using LC/ESI MS/MS. Reaction of BDO with A resulted in the formation of seven A-BDO adducts with MH+ ions at m/z 206. Two of these adducts were observed in the ethanol supernatant. In addition, two formaidopyrimidine (FAPY)-adducts were detected with MH+ ions at m/z 224 in DNA hydrolysate. These data are consistent with formation of regioisomeric N-7 or N-9 adducts which can be released by spontaneous depurination or ring opening. Four other A-BDO adducts were detected in DNA hydrolysate. Similar adducts were observed for Gua. This new methodology permits simultaneous determination of initially formed adducts as well as those arising as a consequence of depurination. The latter adducts provide the opportunity for monitoring exposure to BDO through analysis of N-7 or N-3 adducts excreted in the urine.
journal_name
Toxicologyjournal_title
Toxicologyauthors
Kambouris SJ,Chaudhary AK,Blair IAdoi
10.1016/0300-483x(96)03468-3subject
Has Abstractpub_date
1996-10-28 00:00:00pages
331-5issue
1-3eissn
0300-483Xissn
1879-3185pii
0300-483X(96)03468-3journal_volume
113pub_type
杂志文章相关文献
TOXICOLOGY文献大全abstract::Arsenic contamination of drinking water is a matter of global concern. Arsenic intake impairs immune responses and leads to a variety of pathological conditions including cancer. In order to understand the intricate tuning of immune responses elicited by chronic exposure to arsenic, a mouse model was established by su...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2016.06.005
更新日期:2016-05-16 00:00:00
abstract::The development of mouse embryos was studied after intraperitoneal injection of nickel chloride in the preimplantation period. A single intraperitoneal injection of NiCl2 . 6H2O in 0.154 M NaCl corresponding to 20 mg/kg body wt was given to groups of female mice on days 1, 2, 3, 4, 5 or 6 of gestation. Control groups ...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(81)90104-9
更新日期:1981-01-01 00:00:00
abstract::Methylmercury is an environmental pollutant that exhibits neurotoxicity when ingested, primarily in the form of neuropathological lesions that localize along deep sulci and fissures, in addition to edematous and inflammatory changes in patient cerebrums. These conditions been known to give rise to a variety of ailment...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2017.09.013
更新日期:2017-12-01 00:00:00
abstract::Maize co-contamination with aflatoxin B1 (AFB1) and fumonisin B1 (FB1) is frequently found in several countries. Although the alterations on nutritional and immunologic parameters induced by these mycotoxins, when administered individually, are partially characterised, little is known about the effects induced in anim...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(02)00603-0
更新日期:2003-04-15 00:00:00
abstract::The susceptibility to hepatocellular carcinoma (HCC) varies greatly within human populations in response to environmental risk agents. The mechanisms underlying differential susceptibility are still largely unknown and need to be clarified to improve HCC chemoprevention and therapeutic treatment. Inbred rodent strains...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2008.09.013
更新日期:2008-12-05 00:00:00
abstract::Rats received 0, 200, 1000 and 5000 ppm metaldehyde in the diet for 2 years. Reproduction studies over three generations using the same dietary levels were carried out. In the third litter of each generation attention was paid to possible embryotoxic or teratogenic effects. The parameters studied included growth, food...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(75)90026-8
更新日期:1975-01-01 00:00:00
abstract::The intravenous toxicity of phytic acid (inositol hexakisphosphate, IHP) has recently become of interest because of the potential for IHP incorporation into red blood cells to achieve a therapeutically useful shift in the hemoglobin-oxygen dissociation curve (Gersonde, K. and Nicolau, C., Blut, 39 (1979) 1). The obser...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(81)90022-6
更新日期:1981-01-01 00:00:00
abstract::In rats, 3 days treatment with paracetamol (1 oral dose of 1 g/kg daily) produced a complete protection against the hepatotoxic actions of a further dose of paracetamol as documented by determination of serum enzyme activities (glutamic-oxaloacetic transaminase, (GOT), glutamic-pyruvic transaminase (GPT), sorbitol deh...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(79)90038-6
更新日期:1979-02-01 00:00:00
abstract::The cytotoxicity and cardiotoxicity of benzoyl mustard (FCE 24517) and epoxamido (FCE 24561) synthetic derivatives of distamycin A were reported in the present study. The 50% inhibiting concentration (IC50) of colony formation of FCE 24517 on human SNB-19 glioblastoma, A2780 ovarian cancer and DU 145 prostate cancer w...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(92)90003-w
更新日期:1992-11-15 00:00:00
abstract::Because occupational hazards of small-scale industries were severe and the occupational health management lagged behind in Shanghai, we selected some districts as experimental units to explore a model of community management and service for the occupational health in small-scale industries for controlling occupational...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2004.01.019
更新日期:2004-05-20 00:00:00
abstract::It is generally believed that estrogen receptor-dependent and -independent pathways are involved in mediating the developmental effects of the synthetic estrogen, diethylstilbestrol (DES). However, the precise role and extent to which each pathway contributes to the resulting pathologies remains unknown. We have emplo...
journal_title:Toxicology
pub_type: 杂志文章,评审
doi:10.1016/j.tox.2004.06.046
更新日期:2004-12-01 00:00:00
abstract::Testicular injury is often observed in drug development. Serum hormones are usually used as noninvasive biomarkers for testicular injury; however, their sensitivities are low. Therefore, it is difficult to monitor testicular injury in drug development. In recent years, molecules in body fluid exosomes have attracted a...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2020.152490
更新日期:2020-07-01 00:00:00
abstract::In urban areas with a predominance of early to mid-20th century housing stock, islands of children possessing blood lead levels (PbB) in excess of CDC guidelines (>10μg/dL) exist. Many of these children are also exposed to environmental tobacco smoke (ETS). The current study examined the impact of Pb-exposure (PbB lev...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2011.10.013
更新日期:2012-01-27 00:00:00
abstract::The effect of fly ash inhalation on xenobiotic metabolizing enzymes and heme metabolism in lung and liver has been studied in rats. Fly ash inhalation induced pulmonary and hepatic cytochrome P-450 content, aryl hydrocarbon hydroxylase and glutathione S-transferase activity. Induction of cytochrome P-450 was accompani...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(89)90215-1
更新日期:1989-05-31 00:00:00
abstract::The effects of triethyl lead chloride (TEL) on the expression of two detoxication enzyme families, glutathione S-transferases (GSTs) and NAD(P)H:quinone oxidoreductase (QR) were determined in rat liver and kidney. Fischer 344 rats were given one intraperitoneal (i.p.) injection of TEL. GST activity, GST isoenzyme leve...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(96)03555-x
更新日期:1997-02-14 00:00:00
abstract::It is accepted practice in presenting organ weight data to express the results relative to the animal's body weight. The data presented suggest that in rats no benefits are obtained from this method either in reducing the variability of the data or in removing bias due to different body weights. By using simulated dat...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(76)90050-0
更新日期:1976-03-01 00:00:00
abstract::Inhibition of regulated exocytosis by botulinum toxins type A and B was studied in chromaffin cells. Both virtually abolished catecholamine release triggered from intact cells by depolarising stimuli, whereas the blockade by type A, but not B, was only partial after cell permeabilisation and direct stimulation of exoc...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(02)00453-5
更新日期:2002-12-27 00:00:00
abstract::Reactive isomeric 3- and 4-trifluoromethylanilines (3-,4-TFMA), and control aniline itself, induced the following effects on biosynthesis of DNA in the liver, kidney, thymus and spleen of rats: (a) The administration of 4-TFMA initially suppressed the utilization of labeled thymidine for splenic DNA synthesis during t...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(93)90091-6
更新日期:1993-10-25 00:00:00
abstract::Solar radiation induces acute and chronic reactions in human and animal skin. Chronic repeated exposures are the primary cause of benign and malignant skin tumors, including malignant melanoma. Among types of solar radiation, ultraviolet B (290-320 nm) radiation is highly mutagenic and carcinogenic in animal experimen...
journal_title:Toxicology
pub_type: 杂志文章,评审
doi:10.1016/s0300-483x(03)00150-1
更新日期:2003-07-15 00:00:00
abstract::L-695,256 is a novel 2-fluorenonyl carbapenem antibiotic with significant antimicrobial activity against strains of methicillin-resistant Staphylococci. This prototype compound was administered intravenously to rhesus monkeys (Macaca mulatta) at does of 50 or 200 mg/kg/day for 2 weeks to assess toxicity and found to i...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(96)03305-7
更新日期:1996-04-30 00:00:00
abstract::The effects of ethanol on hamster hepatic and extrahepatic monooxygenases were determined in the present study. Chronic ethanol administration increased cytochrome P-450 (P-450) content and monooxygenase activities towards aniline, N-nitrosodimethylamine, and 7-ethoxyresorufin. In contrast, benzphetamine and benzo(a)p...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(93)90006-e
更新日期:1993-07-28 00:00:00
abstract::The usefulness of urinary taurine as a non-invasive measure of hepatotoxicity of aflatoxin B1 (AFB1) was evaluated: changes in urinary taurine were characterized in a dose-response, acute toxicity experiment and in two sub-chronic, low dose exposure experiments. Urine of young, male, F344 rats was collected for 4 days...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(96)03610-4
更新日期:1997-03-28 00:00:00
abstract::Immunohistochemical methods have been widely used to determine the histogenesis of spontaneous and chemically-induced mouse lung tumors. Typically, antigens for either alveolar Type II cells or bronchiolar epithelial Clara cells are studied. In the present work, the morphological and immunohistochemical phenotype of a...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(03)00060-x
更新日期:2003-05-03 00:00:00
abstract::Chronic beryllium disease (CBD) results from exposure to the light-weight metal beryllium (Be). In vitro stimulation of bronchoalveolar lavage cells from CBD subjects causes the production of high levels of TNF-alpha, IFN-gamma and IL-6. We tested the hypothesis that Be-stimulation might induce the production of TNF-a...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(99)00182-1
更新日期:2000-03-07 00:00:00
abstract::The cardiovascular effects of trazodone, a broad-spectrum antidepressant and its analogue etoperidone, were compared with imipramine, following intravenous infusion in rats. Their effects on electrocardiogram and blood pressure were simultaneously recorded until cardiac arrest. Hypotension was the primary effect of tr...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(78)90065-3
更新日期:1978-06-01 00:00:00
abstract::Rats were exposed to 1.5 +/- 0.1 mg ozone/m3 for 4 days and the activities of glucose-6-P dehydrogenase (G6PDH), glutathione reductase (GR) and glutathione peroxidase (GSHPx) were measured in the cytosolic fraction of lungs from exposed and control rats. Enzyme activities were also measured in isolated alveolar macrop...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(85)90083-6
更新日期:1985-12-01 00:00:00
abstract::The effect of zinc (Zn) on the immunomodulatory properties of cadmium (Cd) was investigated in male Wistar rats. Animals were orally exposed to Cd and or Zn, by stomach tubing for 7 weeks. Response of splenocytes to phytohemagglutinin (PHA) and concanavalin A (Con A), was significantly increased by Zn. When exposed in...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(84)90046-5
更新日期:1984-12-01 00:00:00
abstract::In order to test the hypothesis that Leydig cells are required for the acute toxic effect of cadmium on the testis, adult male rats were treated with the Leydig cell toxin ethane dimethanesulphonate (EDS). Four days later, when all Leydig cells are removed from the testis, the EDS treated and control rats were given a...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(90)90041-e
更新日期:1990-08-01 00:00:00
abstract::Natural phytochemicals derived from dietary sources or medicinal plants have gained significant recognition in the potential management of several human clinical conditions. Much research has also been geared towards the evaluation of plant extracts as effective prophylactic agents since they can act on specific and/o...
journal_title:Toxicology
pub_type: 杂志文章,评审
doi:10.1016/j.tox.2009.10.010
更新日期:2010-12-05 00:00:00
abstract::Drug-induced hypersensitivity syndrome (DIHS) is a severe multi-organ system reaction caused by specific drugs. Many reports have revealed that human herpesvirus 6 (HHV-6) reactivation contributes to the development of DIHS. In addition, recent articles have shown that reactivation of other herpesviruses such as human...
journal_title:Toxicology
pub_type: 杂志文章,评审
doi:10.1016/j.tox.2004.12.013
更新日期:2005-04-15 00:00:00