Enzymatic properties of adult human Sertoli cells in vitro.

Abstract:

:The adult human Sertoli cells produced lactate, estradiol-17beta, transferrin and inhibin; germ cells modulate synthesis of these compounds. In order to study the functional features of human Sertoli cells in vitro, the aim of this study was to measure the lactic dehydrogenase (LDH), gamma-glutamyl transpeptidase (GGT), alkaline phosphatase (ALP) and creatine kinase activities (CK) in primary cultures of Sertoli cells prepared from young men (mean age: 29 years, n = 11). Five LDH isozymes have been found in Sertoli cells, the main fractions being the LDH3 and LDH4; each of them represented 30% of the total LDH activity. Furthermore, CK and ALP activities were measured in Sertoli cells. It is of note that the Sertoli cell ALP activity was 50% lower than that of germ cells. Whatever the Sertoli cell parameter measured herein, there is a great variability between patients and FSH (dbc AMP, as well as retinol, insulin, testosterone) is poorly effective in improving these enzymatic activities in vitro. We have confirmed that GGT was exclusively present in Sertoli cells and thus may be considered as a specific marker. LDH is involved in Sertoli cell glucose transformation and thus provided energetic substrates for germ cells. In contrast, the roles of CK and ALP remains to be clarified. In conclusion, we have demonstrated the existence of several enzymes namely LDH, GGT, ALP and CK in Sertoli cells prepared from adult human testis.

journal_name

Andrologia

journal_title

Andrologia

authors

Carreau S,Drosdowsky MA,Foucault P

doi

10.1111/j.1439-0272.1996.tb02762.x

subject

Has Abstract

pub_date

1996-03-01 00:00:00

pages

89-95

issue

2

eissn

0303-4569

issn

1439-0272

journal_volume

28

pub_type

杂志文章
  • Sperm retrieval in anejaculatory diabetic men who failed in drug treatment and penile vibratory stimulation during blood sugar under control.

    abstract::Ejaculatory dysfunction is an uncommon cause of male infertility. The aim of this study was to explore non-invasive methods for sperm retrieval in anejaculatory diabetic men who failed in drug treatment and penile vibratory stimulation during blood sugar under control. Among 21 anejaculatory diabetic men who had faile...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.12087

    authors: Lu S,Cui Y,Li X,Zhang H,Hu J,Liu J,Chen ZJ

    更新日期:2014-05-01 00:00:00

  • The effects of different dietary protein sources on live weight, sperm quality and the histology of the testes and accessory glands in male rats.

    abstract::This study was aimed at determining the effects of corn and wheat gluten, used as dietary protein sources, on live weight gain, sperm quality and the histology of the testes and accessory glands in male rats. For this purpose, 20-day-old 24 male Wistar albino rats were randomly assigned to three groups. Group 1 (Contr...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13601

    authors: Gumus R,Uslu S,Uslu BA

    更新日期:2020-07-01 00:00:00

  • A new method for evaluation of the acrosome reaction in viable human spermatozoa.

    abstract::The acrosome reaction of human spermatozoa was induced by changes of temperature. Spermatozoa were collected from fertile donors and a patient group, and selected by the "swim-up" method. The spermatozoa were treated in two different ways: Protocol I: 24 hours at room temperature followed by additional incubation at 3...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1991.tb02537.x

    authors: Sanchez R,Toepfer-Petersen E,Aitken RJ,Schill WB

    更新日期:1991-05-01 00:00:00

  • Purification and properties of gamma-glutamyl transpeptidase from human testis.

    abstract::Gamma-glutamyl transpeptidase was purified to apparent homogeneity from human testis by DEAE cellulose, acetone, precipitation, fractionation by ammonium sulphate, Sephacryl S-200 chromatography, Q-Sepharose chromatography and S-Sepharose chromatography following solubilization of the enzyme by Triton X-100. The purif...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1990.tb01973.x

    authors: Yoshida K,Arai K,Kobayashi N,Saitoh H

    更新日期:1990-05-01 00:00:00

  • Acrosome content release in streptolysin O permeabilized mouse spermatozoa.

    abstract::Sperm cell plasma membrane and the outer acrosomal membrane fuse profusely during the acrosome reaction. The process is triggered by extracellular signals that elicit several intracellular events leading ultimately to membrane fusion. We have developed a streptolysin O permeabilizing protocol that selectively affects ...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1996.tb02753.x

    authors: Díaz A,Domínguez L,Fornés MW,Burgos MH,Mayorga LS

    更新日期:1996-01-01 00:00:00

  • A modern approach to the gonadotropin treatment in oligozoospermia.

    abstract::A critical review of the gonadotropin therapy in male infertility or hypofertility is presented. In the author's opinion, the poor and conflicting results so far obtained wih this kind of treatment are mainly due to inadequate selection of the patients admitted to the treatment itself. The author stresses some points ...

    journal_title:Andrologia

    pub_type: 杂志文章,评审

    doi:10.1111/j.1439-0272.1981.tb00030.x

    authors: Isidori A

    更新日期:1981-05-01 00:00:00

  • Icariin improves SHR erectile function via inhibiting eNOS uncoupling.

    abstract::The aim of the study was to investigate whether or not the effect of icariin on erectile function of SHR is associated with inhibition of eNOS uncoupling. Ten 12-week-old male WKY rats and 10 age-matched male SHR were evenly randomised into SHR control group, SHR+ icariin treatment group, WKY control group and WKY+ ic...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13084

    authors: Long H,Jiang J,Xia J,Jiang R

    更新日期:2018-11-01 00:00:00

  • In vitro antioxidant effect of curcumin on human sperm quality in leucocytospermia.

    abstract::Decreased sperm quality was caused by oxidative stress in semen from patients with leucocytospermia. Curcumin is a traditional Chinese herbal monomer extracted from Zingiberaceae turmeric and zedoary turmeric and has antioxidative and anti-inflammatory effects. This study aimed to investigate the effects and specific ...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.12760

    authors: Zhang L,Diao RY,Duan YG,Yi TH,Cai ZM

    更新日期:2017-12-01 00:00:00

  • [Andrological findings in adolescents with retractile testis].

    abstract::Approximately between 9 and 19 years of age 28 adolescents with one and 26 with both retractile testicles were andrologically examined and followed up. Retractile gonads during puberty often remained of subnormal size with the long axis shorter than 34 mm. In both groups in 50 to 60% of cases low sperm values were asc...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:

    authors: Raboch J,Pondĕlicková J

    更新日期:1988-09-01 00:00:00

  • Novel predictive risk factor for Erectile Dysfunction: Serum folic acid.

    abstract::The purpose of this study was to compare the serum Folic Acid (FA) levels in patients with Erectile Dysfunction (ED) and healthy controls and whether levels vary with its severity. The study was carried out on 77 sexually active individuals, out of which 41 complained of ED and 36 were apparently normal. Patients were...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13890

    authors: Gupta A,Priyadarshi S,Vyas N,Sharma G,Swain PK

    更新日期:2021-02-01 00:00:00

  • Protective influence of rosiglitazone against testicular ischaemia-reperfusion injury in rats.

    abstract::Testicular torsion is a urology urgent disease which causes testicular injury and potential sterility. In this study, we explored the protective influence of rosiglitazone on testicular ischaemia-reperfusion damage. There were 28 male Sprague Dawley rats in total, which were assigned randomly to four groups. Group A w...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.12947

    authors: Zheng N,Shao H,Wu D,Shen D,Lin X

    更新日期:2018-01-09 00:00:00

  • Soluble Fibrinogen-like protein 2 plays a role in varicocele induced male infertility.

    abstract::Varicocele is the most common cause of male infertility. Several theories have been proposed to explain how varicocele induces infertility. The role of epididymis in male infertility is not fully well established. Fibrinogen-like protein 2 is one of serine proteases and is a potent coagulant in membranous form and imm...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13626

    authors: Elesawy FM,Abdel Hafeez NA,Abdelsalam OH,Akl EM

    更新日期:2020-07-01 00:00:00

  • The kinetic of acrosome reaction--an additional sperm parameter?

    abstract::Spermatozoa of six men with normozoospermia, oligozoospermia and teratozoospermia were capacitated in vitro in modified Tyrode's medium und evaluated for acrosome reaction using the recently described triple-stain technique. The resulting kinetics illustrate the different capacitation behaviour and ability of spermato...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1985.tb00991.x

    authors: Töpfer-Petersen E,Heissler E,Schill WB

    更新日期:1985-05-01 00:00:00

  • Androgen deficiency impairs erectile function in rats through promotion of corporal fibrosis.

    abstract::The aim of this study was to investigate the underlying mechanism of androgen deficiency inducing corporal fibrosis, thereby causing erectile dysfunction (ED). Forty 12-week-old healthy male rats were divided randomly into four groups: normal control group (Control); castration group (Castration); the other 20 rats we...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.12797

    authors: Cui K,Li R,Chen R,Li M,Wang T,Yang J,Chen Z,Wang S,Liu J,Rao K

    更新日期:2018-02-01 00:00:00

  • Systemic and cavernous plasma levels of neuropeptide Y during sexual arousal in healthy males.

    abstract::Neuropeptide Y (NPY) has been shown to induce contraction of isolated human penile erectile tissue and potentiate the response to noradrenaline. The purpose of our study was to measure in the cavernous and systemic blood of healthy male volunteers the course of NPY through different stages of sexual arousal. Whole blo...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.2011.01182.x

    authors: Ückert S,Soyk L,Trottmann M,Stief CG,Kuczyk MA,Becker AJ

    更新日期:2012-05-01 00:00:00

  • Treatment with levofloxacin does not resolve asymptomatic leucocytospermia--a randomized controlled study.

    abstract::The manual of the World Health Organization defines leucocytospermia as the presence of >1 x 10(6) white blood cells per ml semen. Most authors consider leucocytospermia to be a consequence of inflammation due to bacterial infection. However, the efficacy of antibiotic therapy was not unequivocally demonstrated until ...

    journal_title:Andrologia

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1046/j.1439-0272.2003.00580.x

    authors: Krisp A,Hörster S,Skrzypek J,Krause W

    更新日期:2003-08-01 00:00:00

  • Physiological action of oestradiol on the acrosome reaction in human spermatozoa.

    abstract::The acrosome is a secretory vesicle located in the sperm head. The acrosome reaction consists in the fusion of the sperm plasma membrane with the external acrosomal membrane. It has been observed that this reaction does not take place in spermatozoa incubated in cervical mucus, hydrogel that contains high concentratio...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.2007.00814.x

    authors: Vigil P,Toro A,Godoy A

    更新日期:2008-06-01 00:00:00

  • Effect of tadalafil and 3-hydroxy-3-methylglutaryl coenzyme A inhibitor statin on the haemodynamics of cavernous and brachial arteries.

    abstract::Erectile dysfunction (ED) is usually associated with cardiovascular disease and reduced endothelial function. The aim of the present study was to examine the effect of tadalafil and statin on the endothelial function of cavernous and brachial arteries in healthy men and in patients with ED. The cases included in the s...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.12153

    authors: Simsek A,Ozbek E,Oncu M

    更新日期:2014-09-01 00:00:00

  • Could analysis of testis-specific genes, as biomarkers in seminal plasma, predict presence of focal spermatogenesis in non-obstructive azoospermia?

    abstract::Cell-free seminal mRNA (cfs-mRNA) exists in the human ejaculate that is proposed as a potential noninvasive procedure to prognosis pathophysiological conditions. This study applied cfs-mRNA of ESX1, ZMYND15 and its target haploid genes (TNP1 and PRM1) to identify the presence of spermatozoa in men with azoospermia. Th...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13483

    authors: Hashemi MS,Mozdarani H,Ghaedi K,Nasr-Esfahani MH

    更新日期:2020-03-01 00:00:00

  • Separation of interstitial and tubular cells of human testis by means of an anti-substance P-antiserum. Androstane-3 beta, 17 beta-diol is exclusively formed by seminiferous tubules.

    abstract::Leydig cells and seminiferous tubules of human testicular tissue were successfully separated by means of an anti-substance P-antiserum. On incubation with (14C)-testosterone or (14C)-dihydrotestosterone (DHT) it could be shown that besides DHT and 5 alpha-androstane-3 alpha, 17 beta-diol (3 alpha-diol) also 5 alpha-an...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:

    authors: Tamm J,Volkwein U,Schulze W

    更新日期:1990-01-01 00:00:00

  • Associations of telomere length in risk and recurrence of prostate cancer: A meta-analysis.

    abstract::Over the past decades, there is an increasing number of association studies of telomere length (TL) and the risk and recurrence of prostate cancer (PCa), but the results are inconsistent. Hence, we identify the relevant studies published in English on or before 10 January 2019 conducting a literature review in the ele...

    journal_title:Andrologia

    pub_type: 杂志文章,meta分析

    doi:10.1111/and.13304

    authors: Hu R,Hua XG,Jiang QC

    更新日期:2019-08-01 00:00:00

  • The zona pellucida 'receptors' ZP1, ZP2 and ZP3.

    abstract::The male component that is necessary for successful reproduction depends on a large variety of biological processes working in concert. The sperm-egg interaction occurs through complementary molecules and is an obligatory process for successful fertilization. However, this complex phenomenon and its molecular mechanis...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:

    authors: Hinsch KD,Hinsch E

    更新日期:1999-09-01 00:00:00

  • Positive effect of progesterone on motility and velocity of fresh, vitrified without permeable cryoprotectants and frozen with permeable cryoprotectants human spermatozoa.

    abstract::Steroid hormone progesterone has been found to play an important role in the migration of spermatozoa through the reproductive tract, as well as to induce hyperactive motility and increase sperm velocity. The aim of this study was to examine whether progesterone could induce beneficial effects in vitrified and slow-fr...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13133

    authors: Saymé N,Dite L,Krebs T,Kljajić M,Maas DHA

    更新日期:2018-12-01 00:00:00

  • Electroejaculation and seminal parameters in bonnet monkeys (Macaca radiata).

    abstract::In the present study, our experience in the collection of semen from adult bonnet monkeys and some of the characteristics of semen are reported. Semen samples were collected by penile stimulation every 15 days over a period of 3 years. The penile method of ejaculation was more consistent with an increased volume of se...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1998.tb01153.x

    authors: Ramesh V,Ramachandra SG,Krishnamurthy HN,Rao AJ

    更新日期:1998-03-01 00:00:00

  • Kisspeptin and RF9 prevent paroxetine-induced changes in some parameters of seminal vesicle fluid in the male rats.

    abstract::The aim of the study was to examine possible impacts of paroxetine and agomelatine on the levels of some components that constitute the seminal vesicle fluid. As a second purpose, it was also aimed to examine how possible negative effects induced by paroxetine on seminal vesicle fluid components were affected by kissp...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13538

    authors: Gulcu Bulmus F,Canpolat S,Sahin Z,Bulmus O,Serhatlioglu I,Kelestimur H

    更新日期:2020-05-01 00:00:00

  • Adequacy of hyaluronan binding assay and a new fertility index derived from it for measuring of male fertility potential and the efficacy of supplement therapy.

    abstract::The aim of the study was to statistically prove that the HBA(®) test is an efficient practical method for andrologists to determine the fertility potential as well as to measure the efficiency of oral supplement therapy in case of male infertility. In the study, 175 patients were involved and it also included the foll...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.12296

    authors: Szucs M,Osvath P,Laczko I,Jakab A

    更新日期:2015-06-01 00:00:00

  • Laparoscopy-assisted pelvi-scrotal vasovasostomy.

    abstract::Iatrogenic obstruction of the vas deferens within the inguinal canal can be managed by direct on-site vasovasostomy. However, in cases with large defect of the vas, the anastomosis may be under tension. Dissecting through the site of a previous hernia repair is tedious, and may lead to recurrence of the hernia. The pr...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.2004.00624.x

    authors: Shaeer OK,Shaeer KZ

    更新日期:2004-10-01 00:00:00

  • Anti-androgenic, anti-oxidant and anti-apoptotic effects of the aqueous and methanol extracts of Pterorhachis zenkeri (Meliaceae): Evidence from in vivo and in vitro studies.

    abstract::The aim of this study was to evaluate the effects of Pterorhachis zenkeri (Meliaceae) on sex organ growth in immature male rats and, oxidative stress and apoptosis markers in CCL-97 (R2C) Leydig cells. For the in vivo studies, 70 immature male Wistar rats (n = 10/group) were treated for 2 or 4 weeks with: distilled wa...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13815

    authors: Bonsou Fozin GR,Deeh Defo PB,Wankeu-Nya M,Ngadjui E,Kamanyi A,Watcho P

    更新日期:2020-12-01 00:00:00

  • Response of the testis to gonadotrophin replacement in young hypophysectomized vs. gonadotrophin-releasing hormone antagonist-treated rats.

    abstract::We have studied the response of atrophic Leydig cells to gonadotrophin replacement in young hypophysectomized (HX) and GnRH antagonist (GnRH-ANT)-treated rats. Hypophysectomy was performed at 28 days of age. Age-matched rats were treated with GnRH-ANT from 28 to 51 days of age. From 45 to 51 days of age, animals were ...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1997.tb00468.x

    authors: Gaytan F,Bellido C,Morales C,Aguilar E

    更新日期:1997-03-01 00:00:00

  • Purification and characterization of sperm-coating antigen, identified by a monoclonal antibody.

    abstract::A procedure was designed for purification of a human seminal plasma-specific antigen (HSP-antigen) identified by a monoclonal antibody produced in this laboratory (mAb 4E6). Pooled human seminal plasma was fractionated by consecutively applied methods: affinity chromatography on Lentil lectin sepharose, gel chromatogr...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1993.tb02724.x

    authors: Dimitrova D,Kehayov I,Kyurkchiev S

    更新日期:1993-09-01 00:00:00