Abstract：
:The placenta plays an essential role in fetal growth and the maintenance of pregnancy and its functions are strictly controlled in a stage-specific manner. To gain an insight into placental functions and their regulation, we analyzed the plasma membrane proteins of rat placenta by two-dimensional polyacrylamide gel electrophoresis (2D/E). Plasma membrane fractions of the placenta obtained on days 12, 14, 16, 18 and 20 of pregnancy were purified by Percoll gradient centrifugation, and subjected to 2D/E analysis. After the proteins on the 2D/E gels had been visualized by silver staining, the patterns on the gels at different stages of pregnancy were compared using image analysis software. Proteins within an isoelectric point (pI) range of 4.0 to 7.0 and a molecular weight (Mw) range of 20-100 kDa were analyzed in detail, and about 800 proteins on average were recognized on each gel. Of these, the expression of 150 proteins was found to change dramatically according to the stage of pregnancy. According to their expression patterns, these proteins were categorized into two groups, Group I and Group II. The proteins belonging to Group I showed a higher intensity of expression on day 12 and disappeared on day 20. They included 119 plasma membrane proteins and were divided into five subgroups. Group II, which consisted of three subgroups, included 31 proteins showing a low or negligible expression on day 12 and higher expression on day 20. Most of the other membrane proteins (about 600) were expressed constantly during pregnancy. On the basis of our data, we constructed a database for plasma membrane proteins of the rat placenta.
journal_name
Mol Cell Endocrinoljournal_title
Molecular and cellular endocrinologyauthors
Ishimura R,Noda K,Hattori N,Shiota K,Ogawa Tdoi
10.1016/0303-7207(95)03682-2subject
Has Abstractpub_date
1995-12-29 00:00:00pages
149-59issue
2eissn
0303-7207issn
1872-8057pii
0303720795036822journal_volume
115pub_type
杂志文章abstract：:We hypothesized that DNA methylation is involved in human adrenal functional zonation. mRNAs expression and methylation pattern of RARB, NR4A1 and HSD3B2 genes in human adrenal tissues (HAT) and in pediatric virilizing adrenocortical tumors (VAT) were analyzed. For analysis of the results samples were divided into 3 a...
journal_title：Molecular and cellular endocrinology
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更新日期：1982-04-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/s0303-7207(99)00070-2
更新日期：1999-07-20 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
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更新日期：1996-10-30 00:00:00
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pub_type： 杂志文章
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更新日期：1986-06-01 00:00:00
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更新日期：2011-07-04 00:00:00
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pub_type： 杂志文章
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更新日期：2000-02-25 00:00:00
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pub_type： 杂志文章
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更新日期：2015-11-05 00:00:00
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更新日期：1993-01-01 00:00:00
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更新日期：2014-08-25 00:00:00
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pub_type： 杂志文章
doi：10.1016/0303-7207(90)90100-m
更新日期：1990-08-20 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.mce.2004.08.002
更新日期：2005-01-14 00:00:00
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pub_type： 杂志文章
doi：10.1016/s0303-7207(01)00537-8
更新日期：2001-07-05 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
doi：10.1016/s0303-7207(02)00270-8
更新日期：2002-11-29 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2003.12.002
更新日期：2004-02-12 00:00:00
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pub_type： 杂志文章
doi：10.1016/0303-7207(91)90010-p
更新日期：1991-11-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
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更新日期：1990-01-02 00:00:00
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pub_type： 杂志文章
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更新日期：1988-02-01 00:00:00
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更新日期：1999-10-25 00:00:00
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pub_type： 杂志文章
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更新日期：1999-08-20 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2007.10.013
更新日期：2008-02-13 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
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更新日期：1998-10-25 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(95)03544-h
更新日期：1995-04-28 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(87)90180-8
更新日期：1987-10-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2019.110666
更新日期：2020-02-15 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.mce.2012.11.019
更新日期：2013-05-22 00:00:00
abstract：:The NEDD4L gene encodes a ubiquitin ligase that targets the epithelial sodium channel for degradation. A search for transcripts whose levels increase following androgen treatment of LNCaP human prostate cancer cells led to the isolation of three new NEDD4L transcripts designated NEDD4Lf, NEDD4Lg and NEDD4Lh. The three...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2003.08.009
更新日期：2003-11-28 00:00:00
abstract：:17beta-hydroxysteroid dehydrogenase type 1 (17β-HSD1) is a steroidal enzyme which, in breast cancer cells, mainly synthesizes 17-beta-estradiol (E2), an estrogenic hormone that stimulates breast cancer cell growth. We previously showed that the enzyme increased breast cancer cell proliferation via a dual effect on E2 ...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2016.08.026
更新日期：2017-01-05 00:00:00
abstract：:Secretogranin I (SgI; chromogranin B) belongs to a class of acidic tyrosine-sulfated secretory proteins believed to play a role in the secretory process of endocrine cells. Our aim here was to compare the levels of SgI mRNA to that of prolactin (PRL) and growth hormone (GH), using rat pituitary cell lines. As far as t...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(91)90141-e
更新日期：1991-09-01 00:00:00
