Analysis of EGFR, TGF-alpha, neu and c-myc in 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine-induced mammary tumors using RT-PCR.


:2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), a mutagen found in cooked meat, has been shown to induce mammary gland tumors in rats. Our laboratory recently observed that a high fat diet enhances the incidence and severity of PhIP-induced mammary gland cancer in rats. In the current study, reverse transcription followed by polymerase chain reaction amplification was used to determine whether EGFR, TGF-alpha, neu and c-myc are differentially expressed in PhIP-induced mammary gland tumors classified histologically as benign or malignant and to evaluate whether dietary fat intake influences the expression of these genes. Of 23 total PhIP-induced mammary tumors examined, 43%, 57% and 74% had increased expression of EGFR, TGF-alpha and neu mRNA respectively. Increased expression of these genes appeared to be consistently present in tumors displaying papillomatosis. In contrast, to the other three genes, c-myc mRNA levels were infrequently elevated. The percentage of dietary fat did not appear to influence the expression of EGFR, TGF-alpha or neu in either tumors or mammary gland from control rats. However, the levels of c-myc mRNA were 1.8- and 2.9-fold higher in the control mammary gland and benign PhIP-induced tumors respectively in rats fed the high-fat diet than in rats fed the low-fat diet, suggesting a slight effect of dietary fat (P < 0.08) on c-myc expression. These results suggest that increased expression of EGFR, TGF-alpha and especially neu is associated with PhIP-induced mammary gland cancer in rats.






Davis CD,Snyderwine EG




Has Abstract


1995-12-01 00:00:00












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    pub_type: 杂志文章


    authors: Leng S,Stidley CA,Bernauer AM,Picchi MA,Sheng X,Frasco MA,Van Den Berg D,Gilliland FD,Crowell RE,Belinsky SA

    更新日期:2008-07-01 00:00:00

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    abstract::Progression of hepatic neoplasia was assessed in medaka (Oryzias latipes) following aqueous exposure to diethylnitrosamine (DEN). Larvae (2 weeks old) were exposed to 350 or 500 p.p.m. DEN for 48 h, while adults (3-6 months old) were exposed to 50 p.p.m. DEN for 5 weeks. Fish were maintained as long as possible to det...


    pub_type: 杂志文章


    authors: Okihiro MS,Hinton DE

    更新日期:1999-06-01 00:00:00

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    pub_type: 杂志文章


    authors: Purow BW,Sundaresan TK,Burdick MJ,Kefas BA,Comeau LD,Hawkinson MP,Su Q,Kotliarov Y,Lee J,Zhang W,Fine HA

    更新日期:2008-05-01 00:00:00

  • Metabolism and macromolecular interaction of the tobacco-specific carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone in cultured explants and epithelial cells of human buccal mucosa.

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    pub_type: 杂志文章


    authors: Liu Y,Sundqvist K,Belinsky SA,Castonguay A,Tjälve H,Grafström RC

    更新日期:1993-11-01 00:00:00

  • Critical role of oxidative stress and sustained JNK activation in aloe-emodin-mediated apoptotic cell death in human hepatoma cells.

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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Díez-Fernández C,Sanz N,Alvarez AM,Wolf A,Cascales M

    更新日期:1998-10-01 00:00:00

  • Enhancement by nickel(II) and L-histidine of 2'-deoxyguanosine oxidation with hydrogen peroxide.

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    pub_type: 杂志文章


    authors: Dutta AK,Misra M,North SL,Kasprzak KS

    更新日期:1992-02-01 00:00:00

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    authors: Ayrton AD,Smith JN,Ioannides C

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    authors: Wei H,Cai Q,Rahn RO

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    pub_type: 杂志文章


    authors: Bermudez E,Allen PF

    更新日期:1984-11-01 00:00:00

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    pub_type: 杂志文章


    authors: Wild D,Watkins BE,Vanderlaan M

    更新日期:1991-06-01 00:00:00

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    authors: Flaks A,Flaks B

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Pool BL,Bertram B,Wiessler M

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    pub_type: 杂志文章


    authors: Laib RJ,Klein KP,Bolt HM

    更新日期:1985-01-01 00:00:00