Abstract:
:2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), a mutagen found in cooked meat, has been shown to induce mammary gland tumors in rats. Our laboratory recently observed that a high fat diet enhances the incidence and severity of PhIP-induced mammary gland cancer in rats. In the current study, reverse transcription followed by polymerase chain reaction amplification was used to determine whether EGFR, TGF-alpha, neu and c-myc are differentially expressed in PhIP-induced mammary gland tumors classified histologically as benign or malignant and to evaluate whether dietary fat intake influences the expression of these genes. Of 23 total PhIP-induced mammary tumors examined, 43%, 57% and 74% had increased expression of EGFR, TGF-alpha and neu mRNA respectively. Increased expression of these genes appeared to be consistently present in tumors displaying papillomatosis. In contrast, to the other three genes, c-myc mRNA levels were infrequently elevated. The percentage of dietary fat did not appear to influence the expression of EGFR, TGF-alpha or neu in either tumors or mammary gland from control rats. However, the levels of c-myc mRNA were 1.8- and 2.9-fold higher in the control mammary gland and benign PhIP-induced tumors respectively in rats fed the high-fat diet than in rats fed the low-fat diet, suggesting a slight effect of dietary fat (P < 0.08) on c-myc expression. These results suggest that increased expression of EGFR, TGF-alpha and especially neu is associated with PhIP-induced mammary gland cancer in rats.
journal_name
Carcinogenesisjournal_title
Carcinogenesisauthors
Davis CD,Snyderwine EGdoi
10.1093/carcin/16.12.3087subject
Has Abstractpub_date
1995-12-01 00:00:00pages
3087-92issue
12eissn
0143-3334issn
1460-2180journal_volume
16pub_type
杂志文章相关文献
CARCINOGENESIS文献大全abstract::A method was devised to quantitate N-acetoxy-N-acetyl-2-aminofluorene (N-AcO-AAF) induced crosslinking of the ionic complex between [methyl-3H]thymidine labeled pBR322 DNA and lysozyme. This involved chromatography of the modified complex on Sephadex CM 25 with a high salt buffer that dissociated the non-covalently bo...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/4.5.501
更新日期:1983-01-01 00:00:00
abstract::Folate and vitamin D have been shown to be influenced by ultraviolet (UV) radiation. UVA radiation can break down plasma folate, whereas vitamin D can be synthesized in UVB-exposed skin. Folate metabolism is involved in DNA synthesis and repair, and vitamin D processes anti-proliferative effects. The functions of both...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgl156
更新日期:2007-02-01 00:00:00
abstract::Patients with inflammatory bowel diseases are at increased risk for colorectal cancer. Pharmacological inhibition of cyclooxygenase (COX) function exacerbates symptoms in colitis patients. Animal models of colitis using Cox-2-knockout mice and COX inhibitors also indicate that COX-2 has a protective role against colon...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgq268
更新日期:2011-03-01 00:00:00
abstract::1,3-Dimethyl-3-phenyl-1-nitrosourea (DMPNU) shows no spontaneous decomposition in aqueous buffered solution (pH 7.0) at 37 degrees C over a measuring period of 10 days, but it is directly genotoxic in all assays applied. This compound induces base substitutions in Salmonella typhimurium (especially in the plasmid harb...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/4.4.409
更新日期:1983-01-01 00:00:00
abstract::A two-stage carcinogenesis experiment was performed in Syrian golden hamsters using a single intragastric initiation with 10 mg/kg body weight of 7,12-dimethylbenz[a]anthracene (DMBA) and repetitive topical promotion on the back skin with two different doses (80 and 160 mg, respectively) of benzoyl peroxide in 1 ml ac...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/8.3.479
更新日期:1987-03-01 00:00:00
abstract::Herbacetin is a flavonol compound that is found in plants such as flaxseed and ramose scouring rush herb, it possesses a strong antioxidant capacity, and exerts anticancer effects on colon and breast cancer. However, the effect of herbacetin on skin cancer has not been investigated. Herein, we identified herbacetin as...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgx082
更新日期:2017-10-26 00:00:00
abstract::In this study, we determined the wavelength dependence of u.v.-induced pyrimidine dimer formation, cell killing and mutation induction in human diploid skin fibroblasts. Pyrimidine dimers were quantified using the T4 endonuclease V assay, cell killing was measured as loss of colony forming ability and mutation inducti...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/7.11.1829
更新日期:1986-11-01 00:00:00
abstract::The anti-neoplastic agent procarbazine is genetically active in a variety of short term mutagenicity tests, and it also possesses carcinogenic and teratogenic potential. This compound has consistently yielded false negative results in in vitro microbial mutagenicity tests in the presence and absence of mammalian metab...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/4.3.347
更新日期:1983-01-01 00:00:00
abstract::It is now unanimously accepted that neoplastic cells tend to become less susceptible to the growth regulatory effects of transforming growth factor-beta1 (TGF-beta1), mainly because of reduced expression and/or activity of TGF-beta1-specific receptors, as reported for many human cancers including colon cancer. Consequ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgn106
更新日期:2008-05-01 00:00:00
abstract::Pirh2 is a Ring-H2 domain containing E3 ubiquitin ligase that targets several important tumor suppressor genes for proteasomal degradation. Overexpression of Pirh2 is frequently detected in many clinical tumor tissues including hepatocellular carcinoma (HCC). However, the molecular mechanism of Pirh2 activation in tum...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgs162
更新日期:2012-08-01 00:00:00
abstract::Oltipraz (4-methyl-5-(2-pyrazinyl)-1,2-dithiole-3-thione) (OPZ) is recognized as a potent chemoprotective agent against chemical-induced carcinogenesis in several animal models and is thought to act mainly by inducing phase II conjugating together with inhibiting phase I detoxication enzymes. The present study was und...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/18.11.2113
更新日期:1997-11-01 00:00:00
abstract::Flow cytometric analysis of fibroblasts, normal breast epithelial cells and breast or other cancer cell lines identified variation in the abilities of cell lines to undergo cell cycle arrest as a response to hypoxia. Human mammary epithelial cells (HMEC), normal fibroblasts (Hs68 and WI38), HeLa cervical carcinoma and...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgh274
更新日期:2004-12-01 00:00:00
abstract::A plant polysaccharide, Aloe gel extract, was reported to have an inhibitory effect on benzo[a]pyrene (B[a]P)-DNA adduct formation in vitro and in vivo. Hence, chemopreventive effects of plant polysaccharides [Aloe barbadensis Miller (APS), Lentinus edodes (LPS), Ganoderma lucidum (GPS) and Coriolus versicolor (CPS)] ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/20.8.1637
更新日期:1999-08-01 00:00:00
abstract::The purpose of this work was to study the relative activities and stabilities of phase-I and phase-II drug metabolizing enzymes in incubation mixtures used in vitro genotoxicity testing in order to optimize the conditions of the assay, increase sensitivity and eliminate false negative results. Cytochrome P-450, NADPH-...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/8.9.1179
更新日期:1987-09-01 00:00:00
abstract::A topical application of a chalcone derivative, 4,2',4'-trihydroxychalcone (isoliquiritigenin) inhibited epidermal ornithine decarboxylase (ODC) induction and ear edema formation, i.e. inflammation, caused by a topical application of 12-O-tetradecanoylphorbol-13-acetate (TPA) in CD-1 mice. In addition, isoliquiritigen...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/12.2.317
更新日期:1991-02-01 00:00:00
abstract::Purified human alpha-thrombin stimulates phosphoinositide turnover as a necessary step in its stimulation of fibroblast cell proliferation. Since phosphoinositide turnover releases diacylglycerol, which activates protein kinase C, we postulated that long-term exposure to thrombin might promote cellular transformation ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/13.1.1
更新日期:1992-01-01 00:00:00
abstract::Naturally occurring antioxidants are extensively studied for their capacity to protect organisms and cells from damage induced by oxygen reactive species. In fact, oxidative stress is considered a cause of aging, degenerative disease and cancer. We have focused our attention on two agents, ascorbic acid and beta-carot...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/18.1.223
更新日期:1997-01-01 00:00:00
abstract::The MDM2 protein negatively regulates p53 expression level in modulating DNA repair, cell-cycle control, cell growth and apoptosis. Polymorphisms in the promoter region of the MDM2 gene have been shown to alter protein expression and may, thus, play a role in carcinogenesis. To test our hypothesis that the MDM2 promot...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgl047
更新日期:2006-10-01 00:00:00
abstract::There is indirect evidence to suggest that the excision-repair mechanism responsible for the removal of UV-induced thymine dimers may also play a role in the repair of cis-diamminedichloroplatinum(II) (cis-DDP)-induced DNA adducts in both bacteria and mammalian cells. It was hypothesized that UV dimers and cis-DDP add...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/10.8.1465
更新日期:1989-08-01 00:00:00
abstract::Alk(en)yl trisulfides (R-SSS-R') are organosulfur compounds produced by crushed garlic and other Allium vegetables. We found that these compounds exhibit potent anticancer effects through the reaction with microtubules, causing cell cycle arrest. Nine alk(en)yl trisulfides including dimethyl trisulfide, diethyl trisul...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgn124
更新日期:2008-07-01 00:00:00
abstract::High intake of red meat is associated with an increased risk of colon cancer. It has been suggested that fat from red meat is responsible, because high fat intake increases the concentration of cytotoxic lipids in the colon. Experimental studies have not unequivocally supported such a role for fat, however. Recently, ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/21.10.1909
更新日期:2000-10-01 00:00:00
abstract::The role of O-GlcNAc transferase (OGT) in gene regulation and tumor invasion is poorly understood. Here, we have identified several previously undiscovered OGT-interacting proteins, including the PRMT5/WDR77 complex, the PRC2 complex, the ten-eleven translocation (TET) family, the CRL4B complex and the nucleosome remo...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgy097
更新日期:2018-10-08 00:00:00
abstract::Two new mouse epidermal cell lines have been isolated and characterized as target cells for three chemical carcinogens. The ability to grow these cells at low density (approximately 5 clonogenic cells/cm2) has permitted more precise quantitation of chemical carcinogen-induced changes in epidermal differentiation. The ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/6.9.1245
更新日期:1985-09-01 00:00:00
abstract::In 2000, Douglas Hanahan and Robert Weinberg published a review detailing the six hallmarks of cancer. These are six phenotypes that a tumour requires in order to become a fully fledged malignancy: persistent growth signals, evasion of apoptosis, insensitivity to anti-growth signals, unlimited replicative potential, a...
journal_title:Carcinogenesis
pub_type: 杂志文章,评审
doi:10.1093/carcin/bgp070
更新日期:2009-08-01 00:00:00
abstract::In order to test the controversially discussed hypothesis that oval cells are part of a liver stem cell compartment and can give rise to cholangiocellular as well as hepatocellular carcinomas in the course of liver carcinogenesis, we transfected an oval cell line established in our laboratory with an oncogenically act...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/17.12.2635
更新日期:1996-12-01 00:00:00
abstract::Colorectal cancer (CRC) is the third most common cancer worldwide. Chronic inflammation appears to enhance the risk of CRC. Emerging evidence has suggested that epigenetic mechanisms play an important role in CRC. Aspirin [acetylsalicylic acid (ASA)] has been shown to prevent CRC; however, the epigenetic mechanisms of...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgw042
更新日期:2016-06-01 00:00:00
abstract::Four monoclonal antibodies, PhIP-1, -2, -3 and -4, have been produced that bind to PhIP (2-amino-1-methyl-6-phenyl-imidazo[4,5-b]pyridine), a mutagenic aromatic amine produced in meat by cooking. The immunogen was an analog of PhIP conjugated to a carrier protein through the 2-amino group of PhIP, leaving the pyridine...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/10.12.2215
更新日期:1989-12-01 00:00:00
abstract::When hepatocytes isolated from mouse liver are cultivated in vitro, a small fraction of cells can survive, forming colonies, while most cells die within a few weeks. We compared the colony forming capacity of hepatocytes in three mouse strains; two strains susceptible for hepatocarcinogenesis, C3H/HeJ (C3) and DBA/2J ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/19.6.1103
更新日期:1998-06-01 00:00:00
abstract::Quantum and molecular mechanical calculations were employed to examine the effect on binding energies and structure of methylation and ethylation at O6-guanine in double-stranded DNA. Ab initio quantum chemical calculations (STO-3G, 3-21G) were initially used to pseudo-optimize the structure of the 9-methyl derivative...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/9.9.1553
更新日期:1988-09-01 00:00:00
abstract::Several studies have shown that hexavalent chromium [Cr(VI)] induces apoptosis in a variety of in vitro test systems. We instilled intra-tracheally either saline or sodium dichromate (0.25 mg/kg body weight), for three consecutive days, to Sprague-Dawley rats. TUNEL analyses showed a marked increase of the apoptotic i...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/23.4.587
更新日期:2002-04-01 00:00:00