Transport and metabolism of adenosine in the perfused human placenta.


:Uptake and metabolism of adenosine by human placenta were studied using the single-circulation paired-tracer technique. When isolated cotyledons were perfused through the fetal (basal) circulation at mean pressures of 36 +/- 3.3 mmHg and mean flow rates of 6.6 +/- 0.3 ml/min the maximal [3H]adenosine uptake was 51.3 +/- 3.9 per cent. The uptake was not changed when the vascular resistance was pharmacologically increased. Adenosine uptake was significantly inhibited by adenosine, inosine and nitrobenzylthioinosine (NBMPR), but was unaffected by hypoxanthine. The kinetic analysis of adenosine transport showed it to be a saturable and, Na(+)-independent process, with a Km of 60.8 microM and a Jmax of 0.148 mumol/min. Thin layer chromatographic analysis showed that about 65 per cent of [3H]adenosine was metabolized (10-30 sec) in a single passage through the fetoplacental circulation. [3H]hypoxanthine and [3H]adenine were the major products recovered in the venous perfusate. In the presence of NBMPR the fractional recovery of [3H]adenine and [3H]phosphorylated derivatives was reduced while that of [3H]hypoxanthine was increased. These overall results show that the uptake of adenosine is a Na(+)-independent, NBMPR-sensitive, carrier-mediated process, which appears to be specific for nucleosides, and suggests that metabolization of adenosine proceeds both intra- and extracellularly.






Acevedo CG,Rojas S,Ramirez M,Bravo I




Has Abstract


1995-10-01 00:00:00
















  • Placenta-bound and body fluid PP13 and its mRNA in normal pregnancy compared to preeclampsia, HELLP and preterm delivery.

    abstract:OBJECTIVES:To compare the distribution of placental protein 13 (PP13) in fetal and maternal blood and amnionic fluid and to correlate it with PP13 protein and mRNA in the placenta. METHODS:Umbilical arterial serum, amnionic fluid, maternal venous serum and placental tissues were collected from normal outcome pregnanci...


    pub_type: 杂志文章


    authors: Sammar M,Nisemblat S,Fleischfarb Z,Golan A,Sadan O,Meiri H,Huppertz B,Gonen R

    更新日期:2011-02-01 00:00:00

  • Investigation of the rate-limiting step in the synthesis of acetylcholine by the human placenta.

    abstract::The uptake of [3H]choline and its conversion to [3H]acetylcholine were investigated in term human placental tissue in vitro. Although the net uptake of [3H]choline increased throughout a 45 min incubation period, intracellular [3H]choline levels reach a plateau after 2 min. There was a constant increase in [3H]acetylc...


    pub_type: 杂志文章


    authors: Leventer SM,Rowell PP

    更新日期:1984-05-01 00:00:00

  • ATP independent calcium transport and binding by basal plasma membrane of human placenta.

    abstract::The transport of large amounts of Ca2+ by the plasma membranes of human placental syncytiotrophoblast is essential to the mineralization of the growing fetal skeleton. We have investigated transport by the basal (fetal-facing) plasma membrane (BPM). Ca2+ was taken up by purified BPM vesicles in a time-dependent manner...


    pub_type: 杂志文章


    authors: Kamath SG,Haider N,Smith CH

    更新日期:1994-02-01 00:00:00

  • Alpha-fetoprotein gene expression in early and full-term human trophoblast.

    abstract::Alpha-fetoprotein (AFP) is a major serum glycoprotein synthesized during fetal life mainly by the yolk sac and the fetal liver. At term, it reaches high concentrations in the maternal intervillous blood, which is in direct contact with the placental trophoblastic microvillous membrane, and this suggests the placental ...


    pub_type: 杂志文章


    authors: Lafuste P,Robert B,Mondon F,Danan JL,Rossi B,Duc-Goiran P,Mignot TM,Nunez EA,Benassayag C,Ferré F

    更新日期:2002-09-01 00:00:00

  • Altered maternal and placental lipid metabolism and fetal fat development in obesity: Current knowledge and advances in non-invasive assessment.

    abstract::Abnormal maternal lipid profiles, a hallmark of increased maternal adiposity, are associated with pregnancy complications such as preeclampsia and gestational diabetes, and offspring long-term metabolic health is impacted as the consequence of altered fetal growth, physiology and often iatrogenic prematurity. The meta...


    pub_type: 杂志文章,评审


    authors: Delhaes F,Giza SA,Koreman T,Eastabrook G,McKenzie CA,Bedell S,Regnault TRH,de Vrijer B

    更新日期:2018-09-01 00:00:00

  • SNAT expression in rat placenta.

    abstract::Amino acid transport System A (SysA) activity is present within the rodent and human placentas. Inhibition of this transport system is associated with fetal growth retardation. Several cDNAs encoding SysA transport proteins have been discovered, and their presence documented within the human placenta. We have demonstr...


    pub_type: 杂志文章


    authors: Novak D,Lehman M,Bernstein H,Beveridge M,Cramer S

    更新日期:2006-04-01 00:00:00

  • Endothelium-derived relaxing factor and cyclic GMP-dependent vasorelaxation in human chorionic plate arteries.

    abstract::Endothelium derived relaxing factor (EDRF), now widely believed to be nitric oxide (NO), may play an important part in the control of fetoplacental vascular tone. To further explore this role we have determined the relaxation responses to exogenous NO and examined the temporal relationship between intracellular concen...


    pub_type: 杂志文章


    authors: Hull AD,White CR,Pearce WJ

    更新日期:1994-06-01 00:00:00

  • Interleukin-17 expression in the human placenta.

    abstract::Interleukin (IL)-17 is a proinflammatory cytokine with pleiotropic activities including inducing neovascularization and production of proangiogenic molecules. As pregnancy outcome depends on the balance of Th1-like/Th2-like cytokines and an increased blood supply to the fetoplacental unit, the expression of IL-17 mRNA...


    pub_type: 杂志文章


    authors: Pongcharoen S,Somran J,Sritippayawan S,Niumsup P,Chanchan P,Butkhamchot P,Tatiwat P,Kunngurn S,Searle RF

    更新日期:2007-01-01 00:00:00

  • Identification of two leucine-sensitive lysine transport activities in human placental basal membrane.

    abstract::The recent demonstration of multiple high-affinity leucine-sensitive cationic transport systems prompted this investigation of their role in lysine uptake in basal cell membrane. Transport of lysine by basal membrane was saturable at both 22 and 37 degrees C and linear in time to 1 min and 30 sec, respectively. At 22 ...


    pub_type: 杂志文章


    authors: Furesz TC,Smith CH

    更新日期:1997-11-01 00:00:00

  • Three-dimensional ultrasound measurement of the placental volume in early pregnancy: method and correlation with biochemical placenta parameters.

    abstract::Placental size has been an interesting topic of research for many years. The main aim of this study was to investigate the feasibility of measuring the placental volume at the end of the first trimester using three-dimensional (3D) ultrasound and to correlate these volumes to known placental functional indices and to ...


    pub_type: 杂志文章


    authors: Metzenbauer M,Hafner E,Hoefinger D,Schuchter K,Stangl G,Ogris E,Philipp K

    更新日期:2001-07-01 00:00:00

  • Nutritional composition and heavy metal content of the human placenta.

    abstract::Despite the vital and diverse roles of the placenta, remarkably little is known about its actual composition. Moreover, as placentophagy becomes increasingly popular, understanding the nutritional and heavy metal composition of the human placenta has practical implications. Here we evaluated the carbohydrate, sugar, p...


    pub_type: 杂志文章


    authors: Chang S,Lodico L,Williams Z

    更新日期:2017-12-01 00:00:00

  • Lack of activation of peripheral blood dendritic cells in human pregnancies complicated by intrauterine growth restriction.

    abstract:INTRODUCTION:The state of activation of dendritic cells (DCs) at the feto-maternal interface critically contributes to optimal decidual immune responses needed to support fetal-placental development. We recently demonstrated that during healthy pregnancy also peripheral blood DCs (PBDCs), which are easily accessible, a...


    pub_type: 杂志文章


    authors: Cappelletti M,Giannelli S,Martinelli A,Cetin I,Colombo E,Calcaterra F,Mavilio D,Della Bella S

    更新日期:2013-01-01 00:00:00

  • Lysophosphatidic acid increases the production of pivotal mediators of decidualization and vascularization in the rat uterus.

    abstract:INTRODUCTION:The decidual reaction and the formation of new vessels in the uterus are two crucial processes during embryo implantation. Previously, we observed that lysophosphatidic acid (LPA) increases cyclooxygenase-2 derived - prostaglandin E2 production during implantation in the rat uterus and that it augments the...


    pub_type: 杂志文章


    authors: Beltrame JS,Sordelli MS,Cella M,Perez Martinez S,Franchi AM,Ribeiro ML

    更新日期:2013-09-01 00:00:00

  • Increased matrix metalloproteinases 2 and 9 in placenta of diabetic rats at midgestation.

    abstract::Matrix metalloproteinases (MMPs) are involved in placental remodelling throughout pregnancy. Diabetes mellitus induces alterations in tissue production of NO, a regulator of MMPs activity. The present work evaluates placental and fetal MMPs and NO levels during midpregnancy in neonatal streptozotocin-induced diabetic ...


    pub_type: 杂志文章


    authors: Pustovrh MC,Jawerbaum A,Capobianco E,White V,López-Costa JJ,González E

    更新日期:2005-04-01 00:00:00

  • Region-specific changes in the mRNA and protein expression of LCPUFA biosynthesis enzymes and transporters in the placentae of women with preeclampsia.

    abstract::The biosynthesis and transport of long chain polyunsaturated fatty acids (LCPUFA) require the activity of fatty acid desaturase (FADS) enzymes, fatty acid transport proteins (FATP) and fatty acid binding proteins (FABP). In a previous study we have demonstrated region-specific changes in the LCPUFA levels in preeclamp...


    pub_type: 杂志文章


    authors: Rani A,Chavan-Gautam P,Mehendale S,Wagh G,Mani NS,Joshi S

    更新日期:2020-06-01 00:00:00

  • All-trans-retinoic acid regulates aquaporin-3 expression and related cellular membrane permeability in the human amniotic environment.

    abstract:INTRODUCTION:The aquaporins (AQP1, 3, 8, 9 and 11) are known to be expressed, and involved in the transport of water and small molecules through fetal membranes. To exert these crucial functions, these AQPs have to be finely regulated. All-trans-retinoic acid (atRA) was previously found to regulate some genes in this e...


    pub_type: 杂志文章


    authors: Prat C,Bouvier D,Comptour A,Marceau G,Belville C,Clairefond G,Blanc P,Gallot D,Blanchon L,Sapin V

    更新日期:2015-08-01 00:00:00

  • Placental miR-1301 is dysregulated in early-onset preeclampsia and inversely correlated with maternal circulating leptin.

    abstract:INTRODUCTION:miRNAs are small non-coding RNAs important for the regulation of mRNA in many organs including placenta. Adipokines and specifically leptin are known to be dysregulated in preeclampsia, but little is known regarding their regulation by miRNAs during pregnancy. METHODS:We performed high-throughput sequenci...


    pub_type: 杂志文章


    authors: Weedon-Fekjær MS,Sheng Y,Sugulle M,Johnsen GM,Herse F,Redman CW,Lyle R,Dechend R,Staff AC

    更新日期:2014-09-01 00:00:00

  • Volumes and numbers of intervillous pores and villous domains in placentas associated with intrauterine growth restriction and/or pre-eclampsia.

    abstract::The intrauterine environment has an important influence on placental development. In pre-eclampsia (PE) and intrauterine growth restriction (IUGR), early remodelling of spiral arteries has repercussions for uteroplacental blood flow. The IUGR placenta exhibits compromised growth of villous trees, a smaller intervillou...


    pub_type: 杂志文章


    authors: Rainey A,Mayhew TM

    更新日期:2010-07-01 00:00:00

  • Morphometric studies on villi in human term placentae and the effects of altitude, ethnic grouping and sex of newborn.

    abstract::Stereological methods were used to test whether or not altitudinal, ethnic and sex differences in birthweight in Bolivia are associated with differences in placental morphology. Estimates of the volumes, surface areas, lengths and diameters of villi and of fetal capillaries were obtained by analysing paraffin sections...


    pub_type: 杂志文章


    authors: Jackson MR,Mayhew TM,Haas JD

    更新日期:1987-09-01 00:00:00

  • HLA-G polymorphisms and molecule function--questions and more questions--a review.

    abstract::Human leukocyte antigen (HLA)-G is a non-classical HLA-class I antigen which is predominantly expressed on invasive trophoblastic cells and is postulated to be a mediator of maternal-fetal tolerance. HLA-G interacts with NK cells, can present nonamer peptides and binds CD8 in an analogous manner to classical HLA-I. Th...


    pub_type: 杂志文章,评审


    authors: van der Ven K,Pfeiffer K,Skrablin S

    更新日期:2000-03-01 00:00:00

  • Maternal risk factors associated with persistent placenta previa.

    abstract:INTRODUCTION:Maternal risk factors associated with placenta previa are well documented in the literature. However, there are limited studies identifying maternal characteristics associated with the persistence of placenta previa. The objective of the study was to determine maternal characteristics associated with the p...


    pub_type: 杂志文章


    authors: King LJ,Dhanya Mackeen A,Nordberg C,Paglia MJ

    更新日期:2020-09-15 00:00:00

  • Expression of thioredoxin and thioredoxin reductase in placentae of pregnant mice exposed to lipopolysaccharide.

    abstract::We have previously shown that thioredoxin and thioredoxin reductase were immunohistochemically localized in cytotrophoblasts, decidua and stromal cells in the stem villi of human placenta and that the addition of exogenous thioredoxin and thioredoxin reductase to mitochondrial fractions from human placenta displayed a...


    pub_type: 杂志文章


    authors: Ejima K,Koji T,Nanri H,Kashimura M,Ikeda M

    更新日期:1999-09-01 00:00:00

  • Aspirin inhibits expression of sFLT1 from human cytotrophoblasts induced by hypoxia, via cyclo-oxygenase 1.

    abstract:INTRODUCTION:Elevated circulating soluble FLT1 (sFLT1) levels seen in preeclampsia may play a role in its development. Aspirin is recommended for prevention of preeclampsia. We hypothesized that aspirin may inhibit the production of sFlt1. METHODS:Placentas from women with and without preeclampsia were collected. Prim...


    pub_type: 杂志文章


    authors: Li C,Raikwar NS,Santillan MK,Santillan DA,Thomas CP

    更新日期:2015-04-01 00:00:00

  • Modulation of the novel cannabinoid receptor - GPR55 - during rat fetoplacental development.

    abstract::Decidualization process involves the morphological and functional transformation of endometrial stromal cells into decidual cells. This is a finely regulated process, which involves proliferation and differentiation of stromal cells into decidual cells, which is followed by regression of the decidual tissue, mainly by...


    pub_type: 杂志文章


    authors: Fonseca BM,Teixeira NA,Almada M,Taylor AH,Konje JC,Correia-da-Silva G

    更新日期:2011-06-01 00:00:00

  • Localization of thioredoxin reductase and thioredoxin in normal human placenta and their protective effect against oxidative stress.

    abstract::Recent studies have indicated that oxidative stress is involved in the pathogenesis of pre-eclampsia. Oxidative stress damages systemic tissues, and placental damage may result in intrauterine growth retardation and fetal distress. Thus, this study attempted to elucidate the placental localization of thioredoxin and t...


    pub_type: 杂志文章


    authors: Ejima K,Nanri H,Toki N,Kashimura M,Ikeda M

    更新日期:1999-01-01 00:00:00

  • FLICE-inhibitory protein: expression in early and late gestation human placentas.

    abstract::The apoptosis cascade that plays a central role in normal and pathological processes is strictly controlled, in part by FLIP (Fas-associated death domain-like interleukin-1beta-converting enzyme-inhibitory protein), an inhibitor of caspase-8. Here, we report the expression of long and short isoforms of FLIP mRNAs and ...


    pub_type: 杂志文章


    authors: Ka H,Hunt JS

    更新日期:2006-06-01 00:00:00

  • Aberrantly up-regulated miR-142-3p inhibited the proliferation and invasion of trophoblast cells by regulating FOXM1.

    abstract:INTRODUCTION:Preeclampsia is one of the main causes of morbidity and mortality in pregnant women and mothers. Numerous studies showed that microRNAs (miRNAs) played important roles in the occurrence and development of preeclampsia. However, the regulation of microRNA-142-3p (miR-142-3p) in preeclampsia has not been cla...


    pub_type: 杂志文章


    authors: Mao Y,Hou B,Shan L,Sun X,Wang L

    更新日期:2021-01-06 00:00:00

  • Primate maternal placental angiography.

    abstract:BACKGROUND:In humans, it is known that blood flow is directed to the gravid uterus from two (right and left) pelvic uterine arteries. The extent of supply from the tubo-ovarian anastomosis (joining of the ovarian and uterine arteries) is unknown. The aim of this study was to delineate the arterial blood supply to the p...


    pub_type: 杂志文章


    authors: Balasuriya H,Bell P,Waugh R,Thompson J,Gillin A,Hennessy A,Makris A

    更新日期:2010-01-01 00:00:00

  • Exploring sexual dimorphism in placental circulation at 22-24 weeks of gestation: A cross-sectional observational study.

    abstract:INTRODUCTION:Placental blood flow is closely associated with fetal growth and wellbeing. Recent studies suggest that there are differences in blood flow between male and female fetuses. We hypothesized that sexual dimorphism exists in fetal and placental blood flow at 22-24 weeks of gestation. METHODS:This was a prosp...


    pub_type: 杂志文章


    authors: Widnes C,Flo K,Acharya G

    更新日期:2017-01-01 00:00:00

  • Uptake of 1-methyl-4-phenylpyridinium (MPP+) by the JAR human placental choriocarcinoma cell line: comparison with 5-hydroxytryptamine.

    abstract::The aim of this work was to characterize the uptake of 1-methyl-4-phenylpyridinium (MPP(+)) in the JAR human choriocarcinoma cell line. As JAR cells, as well as the placenta, express the neuronal serotonin transporter (SERT), a comparison between the uptake of (3)H-MPP(+) and (3)H-serotonin ((3)H-5HT) was made. Specif...


    pub_type: 杂志文章


    authors: Martel F,Keating E

    更新日期:2003-04-01 00:00:00