Transport and metabolism of adenosine in the perfused human placenta.


:Uptake and metabolism of adenosine by human placenta were studied using the single-circulation paired-tracer technique. When isolated cotyledons were perfused through the fetal (basal) circulation at mean pressures of 36 +/- 3.3 mmHg and mean flow rates of 6.6 +/- 0.3 ml/min the maximal [3H]adenosine uptake was 51.3 +/- 3.9 per cent. The uptake was not changed when the vascular resistance was pharmacologically increased. Adenosine uptake was significantly inhibited by adenosine, inosine and nitrobenzylthioinosine (NBMPR), but was unaffected by hypoxanthine. The kinetic analysis of adenosine transport showed it to be a saturable and, Na(+)-independent process, with a Km of 60.8 microM and a Jmax of 0.148 mumol/min. Thin layer chromatographic analysis showed that about 65 per cent of [3H]adenosine was metabolized (10-30 sec) in a single passage through the fetoplacental circulation. [3H]hypoxanthine and [3H]adenine were the major products recovered in the venous perfusate. In the presence of NBMPR the fractional recovery of [3H]adenine and [3H]phosphorylated derivatives was reduced while that of [3H]hypoxanthine was increased. These overall results show that the uptake of adenosine is a Na(+)-independent, NBMPR-sensitive, carrier-mediated process, which appears to be specific for nucleosides, and suggests that metabolization of adenosine proceeds both intra- and extracellularly.






Acevedo CG,Rojas S,Ramirez M,Bravo I




Has Abstract


1995-10-01 00:00:00
















  • Lysophosphatidic acid increases the production of pivotal mediators of decidualization and vascularization in the rat uterus.

    abstract:INTRODUCTION:The decidual reaction and the formation of new vessels in the uterus are two crucial processes during embryo implantation. Previously, we observed that lysophosphatidic acid (LPA) increases cyclooxygenase-2 derived - prostaglandin E2 production during implantation in the rat uterus and that it augments the...


    pub_type: 杂志文章


    authors: Beltrame JS,Sordelli MS,Cella M,Perez Martinez S,Franchi AM,Ribeiro ML

    更新日期:2013-09-01 00:00:00

  • The effects of angiogenic growth factors on extravillous trophoblast invasion and motility.

    abstract::There is accumulating evidence that deficient trophoblast invasion of the placental bed spiral arteries is crucial to the pathogenesis of pre-eclampsia and intrauterine growth restriction. However, the factors which regulate the process of trophoblast invasion remain unclear. We have investigated whether extravillous ...


    pub_type: 杂志文章


    authors: Lash GE,Cartwright JE,Whitley GS,Trew AJ,Baker PN

    更新日期:1999-11-01 00:00:00

  • A limitation of the method for siRNA delivery into primary human cytotrophoblast cells.

    abstract::Transfection using cationic lipid reagents such as DharmaFECT2 is an efficient tool for introducing siRNA into primary human cytotrophoblast cells. However, we now report a limitation to the use of this method as the concentration of DharamFECT2 needed for effective siRNA delivery causes ligand-independent activation ...


    pub_type: 杂志文章


    authors: Desforges M,Westwood M

    更新日期:2011-02-01 00:00:00

  • Histochemical localization of glucose residues in the human term placenta.

    abstract::Glucose residues were revealed by light microscopic and fine structural analysis using hexokinase-gold conjugate. In human term placenta specific staining was detected over the stroma of placental villi. Colloidal gold particles were found over the collagen fibrils and reticular lamina of basal membrane. Syncytiotroph...


    pub_type: 杂志文章


    authors: Glazyrin AL,Kolesnikov SI

    更新日期:1993-09-01 00:00:00

  • Effect of oleic acid supplementation on prostaglandin production in maternal endometrial and fetal allantochorion cells isolated from late gestation ewes.

    abstract:INTRODUCTION:Elevated circulating non-esterified fatty acids including oleic acid (OA) are associated with many pregnancy related complications. Prostaglandins (PGs) play crucial roles during parturition. We investigated the effect of OA supplementation on PG production using an in vitro model of ovine placenta. METHO...


    pub_type: 杂志文章


    authors: Cheng Z,Abayasekara DR,Elmes M,Kirkup S,Wathes DC

    更新日期:2015-09-01 00:00:00

  • Umbilical cord as an analytical matrix - A technical note.

    abstract::The umbilical cord (UC) connects the fetal blood supply to the placenta, so is exposed to all systemic endo- and xenobiotics. We have extensive experience using UC as an analytical matrix for detecting and/or quantitating drugs, chemicals and endogenous compounds. This technical note describes advantages (large amount...


    pub_type: 杂志文章


    authors: Price HR,Chehroudi C,Knight SJ,Smith AD,Lai D,Kim H,Wright TE,Coughtrie MW,Collier AC

    更新日期:2020-01-15 00:00:00

  • Human predecidual stromal cells have distinctive characteristics of pericytes: Cell contractility, chemotactic activity, and expression of pericyte markers and angiogenic factors.

    abstract:INTRODUCTION:Human decidual stromal cells (DSCs) play a key role in maternal-fetal interactions. Precursors of DSCs (preDSCs) localize around vessels in both the endometrium and decidua. Previous studies suggested a relationship between preDSCs and pericytes because these cells share a perivascular location, alpha smoo...


    pub_type: 杂志文章


    authors: Muñoz-Fernández R,de la Mata C,Prados A,Perea A,Ruiz-Magaña MJ,Llorca T,Fernández-Rubio P,Blanco O,Abadía-Molina AC,Olivares EG

    更新日期:2018-01-01 00:00:00

  • Alpha-fetoprotein gene expression in early and full-term human trophoblast.

    abstract::Alpha-fetoprotein (AFP) is a major serum glycoprotein synthesized during fetal life mainly by the yolk sac and the fetal liver. At term, it reaches high concentrations in the maternal intervillous blood, which is in direct contact with the placental trophoblastic microvillous membrane, and this suggests the placental ...


    pub_type: 杂志文章


    authors: Lafuste P,Robert B,Mondon F,Danan JL,Rossi B,Duc-Goiran P,Mignot TM,Nunez EA,Benassayag C,Ferré F

    更新日期:2002-09-01 00:00:00

  • Aspirin inhibits expression of sFLT1 from human cytotrophoblasts induced by hypoxia, via cyclo-oxygenase 1.

    abstract:INTRODUCTION:Elevated circulating soluble FLT1 (sFLT1) levels seen in preeclampsia may play a role in its development. Aspirin is recommended for prevention of preeclampsia. We hypothesized that aspirin may inhibit the production of sFlt1. METHODS:Placentas from women with and without preeclampsia were collected. Prim...


    pub_type: 杂志文章


    authors: Li C,Raikwar NS,Santillan MK,Santillan DA,Thomas CP

    更新日期:2015-04-01 00:00:00

  • Placental histological lesions in fetal and neonatal alloimmune thrombocytopenia: A retrospective cohort study of 21 cases.

    abstract:BACKGROUND:Alloimmunization against human platelet antigens (HPAs) can occur prenatally and induce fetal/neonatal alloimmune thrombocytopenia (FNAIT). The aim of this study was to identify placental histological features associated with platelet alloimmunization and their clinical significance. METHODS:This study exam...


    pub_type: 杂志文章


    authors: Dubruc E,Lebreton F,Giannoli C,Rabilloud M,Huissoud C,Devouassoux-Shisheboran M,Allias F

    更新日期:2016-12-01 00:00:00

  • DNA content and ploidy level of bovine placentomal trophoblast giant cells.

    abstract::Cytophotometric measurement of the DNA content of Feulgen-stained nuclei in touch preparations of bovine placentomes (n =5) revealed that 8C nuclei occurred in all, 16C nuclei in two, and 32C nuclei in one specimen. The determination of ploidy level by in situ hybridization with a Y-chromosome specific DNA probe showe...


    pub_type: 杂志文章


    authors: Klisch K,Hecht W,Pfarrer C,Schuler G,Hoffmann B,Leiser R

    更新日期:1999-07-01 00:00:00

  • Potential clinical applications of placental stem cells for use in fetal therapy of birth defects.

    abstract::Placental stem cells are of growing interest for a variety of clinical applications due to their multipotency and ready availability from otherwise frequently discarded biomaterial. Stem cells derived from the placenta have been investigated in a number of disease processes, including wound healing, ischemic heart dis...


    pub_type: 杂志文章,评审


    authors: Vanover M,Wang A,Farmer D

    更新日期:2017-11-01 00:00:00

  • Mononuclear phagocytes in the human placenta.

    abstract::Hofbauer cells have been identified by morphological criteria in human placentae and are considered to be mononuclear phagocytes. An increasing recognition of the morphological heterogeneity of mononuclear phagocytes and of the considerable difficulty in their recognition in histological sections has suggested that th...


    pub_type: 杂志文章


    authors: Wood GW

    更新日期:1980-04-01 00:00:00

  • Placental pathology in congenital rubella.

    abstract::Two groups of placentae from 18 cases of maternal rubella were examined morphologically and virologically. Placentae in Group I (four cases) had a mean gestational age of 21 +/- 1.9 weeks, whilst those in Group 2 (14 cases) had a mean gestational age of 38 +/- 2.8 weeks. A tendency to hypoplasia was observed. The micr...


    pub_type: 杂志文章


    authors: Garcia AG,Marques RL,Lobato YY,Fonseca ME,Wigg MD

    更新日期:1985-07-01 00:00:00

  • Air pollution and pre-eclampsia; associations and potential mechanisms.

    abstract:INTRODUCTION:Air pollution has significant negative health impacts, particularly on the cardiovascular system. The aims of this narrative review were to identify whether there is an association between air pollution and the incidence of pre-eclampsia, and the potential mechanisms by which any effects may be mediated. ...


    pub_type: 杂志文章,评审


    authors: Bearblock E,Aiken CE,Burton GJ

    更新日期:2020-12-18 00:00:00

  • The levels of hypoxia-regulated microRNAs in plasma of pregnant women with fetal growth restriction.

    abstract::MicroRNAs (miRNAs) are short non-coding RNAs that regulate gene expression at the post-transcriptional level. While mostly intracellular, a portion of cellular miRNAs is released to the circulation and their level in the plasma is altered in certain pathological conditions such as cancer, and also during pregnancy. We...


    pub_type: 杂志文章


    authors: Mouillet JF,Chu T,Hubel CA,Nelson DM,Parks WT,Sadovsky Y

    更新日期:2010-09-01 00:00:00

  • Advanced oxidation protein products enhances soluble Fms-like tyrosine kinase 1 expression in trophoblasts: a possible link between oxidative stress and preeclampsia.

    abstract::Accumulation of advanced oxidation protein products (AOPPs) is prevalent in obesity, advanced maternal age, diabetes mellitus, and polycystic ovary syndrome. Alterations in the regulation and signaling of angiogenic pathways have been recognized as a link between these conditions and pre-eclampsia. To investigate the ...


    pub_type: 杂志文章


    authors: Huang QT,Wang SS,Zhang M,Huang LP,Tian JW,Yu YH,Wang ZJ,Zhong M

    更新日期:2013-10-01 00:00:00

  • Classics revisited: C. J. van der Horst on pregnancy and menstruation in elephant shrews.

    abstract:BACKGROUND:Menstruation occurs only in higher primates, some bats, the spiny mouse and the elephant shrew. Our knowledge of the latter species is due to work by C. J. van der Horst. FINDINGS:Changes in the uterine stroma are initially similar in fertile and infertile cycles and are confined to a small area. In pregnan...


    pub_type: 传,历史文章,杂志文章,评审


    authors: Carter AM

    更新日期:2018-07-01 00:00:00

  • Cullins in human intra-uterine growth restriction: expressional and epigenetic alterations.

    abstract::Intra-uterine growth restriction (IUGR) is defined by a restriction of fetal growth during gestation. It is a prevalent significant public health problem that jeopardizes neonatal health but also that can have deleterious consequences later in adult life. Cullins constitute a family of seven proteins involved in cell ...


    pub_type: 杂志文章


    authors: Gascoin-Lachambre G,Buffat C,Rebourcet R,Chelbi ST,Rigourd V,Mondon F,Mignot TM,Legras E,Simeoni U,Vaiman D,Barbaux S

    更新日期:2010-02-01 00:00:00

  • Fractional volume of placental vessels in women with diabetes using a novel stereological 3D power Doppler technique.

    abstract:OBJECTIVES:In maternal diabetes the placenta is large with abnormal vascular development and increased villous volume. We used a novel stereological 3D power Doppler ultrasound technique to investigate differences in-vivo in the placental fractional volume of power Doppler signal (FrVol-PD) between women with and witho...


    pub_type: 杂志文章


    authors: Jones NW,Deshpande R,Mousa HA,Mansell P,Raine-Fenning N,Bugg G

    更新日期:2013-11-01 00:00:00

  • HDlive imaging of intra-amniotic umbilical vein varix with thrombosis.

    abstract::We present a case of intra-amniotic umbilical vein varix with thrombosis using conventional two-dimensional (2D) sonography, power Doppler, three-dimensional (3D) HD-flow, and HDlive at 35 weeks of gestation. 2D sonography showed a large banana-like umbilical cord enlargement (100 × 43.3 × 45.9 mm) including umbilical...


    pub_type: 杂志文章


    authors: Kanenishi K,Nitta E,Mashima M,Hanaoka U,Koyano K,Tanaka H,Hata T

    更新日期:2013-11-01 00:00:00

  • Expression patterns of Notch receptors and their ligands Jagged and Delta in human placenta.

    abstract::The establishment of an appropriate fetomaternal vessel system is a prerequisite for prevention of pregnancy associated pathologies. Notch receptors and ligands are manifoldly involved in vascular development and angiogenesis. To further characterize the process of human placental vasculo- and angiogenesis we investig...


    pub_type: 杂志文章


    authors: Herr F,Schreiner I,Baal N,Pfarrer C,Zygmunt M

    更新日期:2011-08-01 00:00:00

  • Telomere homeostasis in IUGR placentas - A review.

    abstract::Telomeres are nucleoprotein structures located at the termini of chromosomes. They are essential for chromosome stability. Telomeres become shorter due to mitotic cycles and environmental factors. When telomeres are shortened and therefore dysfunctional, cellular senescence occurs and organ dysfunction might develop. ...


    pub_type: 杂志文章,评审


    authors: Biron-Shental T,Sadeh-Mestechkin D,Amiel A

    更新日期:2016-03-01 00:00:00

  • Nitric oxide synthase activity in placentae from women with pre-eclampsia.

    abstract::The syncytiotrophoblast (ST) cell layer of the human villous placenta expresses nitric oxide (NO) synthase. Because NO is a potent relaxant of vascular smooth muscle and inhibitor of platelet activity, we postulated that exaggerated intervillous aggregation of platelets and reduced fetoplacental blood flow in pre-ecla...


    pub_type: 杂志文章


    authors: Conrad KP,Davis AK

    更新日期:1995-12-01 00:00:00

  • Long-chain polyunsaturated fatty acid transport across human placental choriocarcinoma (BeWo) cells.

    abstract::Long-chain polyunsaturated fatty acids (LCPUFAs) such as docosahexaenoic acid (DHA) and arachidonic acid (AA) are essential for proper development of fetal brain and retina. These LCPUFAs are selectively enriched in the fetal circulation compared with the maternal circulation. In the current study we investigated the ...


    pub_type: 杂志文章


    authors: Tobin KA,Johnsen GM,Staff AC,Duttaroy AK

    更新日期:2009-01-01 00:00:00

  • Chronic villitis of unknown etiology and massive chronic intervillositis have similar immune cell composition.

    abstract:INTRODUCTION:Chronic villitis of unknown etiology (CVUE) and massive chronic intervillositis (MCI) are placental lesions associated with infiltration of mononuclear cells in the chorionic villi and the intervillous spaces, respectively. It is not well known whether immune cells in CVUE and MCI have similar phenotypic c...


    pub_type: 杂志文章


    authors: Labarrere CA,Hardin JW,Haas DM,Kassab GS

    更新日期:2015-06-01 00:00:00

  • An analysis using DNA microarray of the time course of gene expression during syncytialization of a human placental cell line (BeWo).

    abstract::Placental trophoblast syncytialization is a unique biological process. We have studied the time course of this process using DNA microarray in a cell model of syncytialization (the cytotrophoblast cell line BeWo following increased intracellular cAMP by forskolin). Total RNA was extracted from BeWo cells and labelled-...


    pub_type: 杂志文章


    authors: Kudo Y,Boyd CA,Sargent IL,Redman CW,Lee JM,Freeman TC

    更新日期:2004-07-01 00:00:00

  • Placental abnormalities associated with isolated single umbilical artery in small-for-gestational-age births.

    abstract:BACKGROUND:Previous studies have shown that pregnancies complicated by placentas with an isolated single umbilical artery (iSUA) are at increased risk for small-for-gestational-age (SGA) births. The etiology of SGA in this population, however, remains unknown. OBJECTIVE:The primary objective of this study was to evalu...


    pub_type: 杂志文章


    authors: Battarbee AN,Palatnik A,Ernst LM,Grobman WA

    更新日期:2017-11-01 00:00:00

  • Subcellular localization of PP5/TFPI-2 in human placenta: a possible role of PP5/TFPI-2 as an anti-coagulant on the surface of syncytiotrophoblasts.

    abstract::Placental protein 5 (PP5)/tissue factor pathway inhibitor-2 (TFPI-2), a serine proteinase inhibitor, is homologous to tissue factor pathway inhibitor (TFPI) and commonly found in peripheral blood of pregnant woman. Although TFPI is well known to be synthesized primarily in endothelium and to play an important role in ...


    pub_type: 杂志文章


    authors: Udagawa K,Yasumitsu H,Esaki M,Sawada H,Nagashima Y,Aoki I,Jin M,Miyagi E,Nakazawa T,Hirahara F,Miyazaki K,Miyagi Y

    更新日期:2002-02-01 00:00:00

  • Confocal and conventional immunofluorescent and immunogold electron microscopic localization of collagen types III and IV in human placenta.

    abstract::Confocal and conventional indirect immunofluorescence and immunogold electron microscopic methods were applied to examine the distribution of extracellular matrix constituents (collagens types III and IV) in the villi of immature and term human placentae. The immunofluorescence study revealed that collagen type III is...


    pub_type: 杂志文章


    authors: Nanaev AK,Rukosuev VS,Shirinsky VP,Milovanov AP,Domogatsky SP,Duance VC,Bradbury FM,Yarrow P,Gardiner L,d'Lacey C

    更新日期:1991-11-01 00:00:00