Abstract:
:Uptake and metabolism of adenosine by human placenta were studied using the single-circulation paired-tracer technique. When isolated cotyledons were perfused through the fetal (basal) circulation at mean pressures of 36 +/- 3.3 mmHg and mean flow rates of 6.6 +/- 0.3 ml/min the maximal [3H]adenosine uptake was 51.3 +/- 3.9 per cent. The uptake was not changed when the vascular resistance was pharmacologically increased. Adenosine uptake was significantly inhibited by adenosine, inosine and nitrobenzylthioinosine (NBMPR), but was unaffected by hypoxanthine. The kinetic analysis of adenosine transport showed it to be a saturable and, Na(+)-independent process, with a Km of 60.8 microM and a Jmax of 0.148 mumol/min. Thin layer chromatographic analysis showed that about 65 per cent of [3H]adenosine was metabolized (10-30 sec) in a single passage through the fetoplacental circulation. [3H]hypoxanthine and [3H]adenine were the major products recovered in the venous perfusate. In the presence of NBMPR the fractional recovery of [3H]adenine and [3H]phosphorylated derivatives was reduced while that of [3H]hypoxanthine was increased. These overall results show that the uptake of adenosine is a Na(+)-independent, NBMPR-sensitive, carrier-mediated process, which appears to be specific for nucleosides, and suggests that metabolization of adenosine proceeds both intra- and extracellularly.
journal_name
Placentajournal_title
Placentaauthors
Acevedo CG,Rojas S,Ramirez M,Bravo Idoi
10.1016/0143-4004(95)90030-6subject
Has Abstractpub_date
1995-10-01 00:00:00pages
611-22issue
7eissn
0143-4004issn
1532-3102pii
0143-4004(95)90030-6journal_volume
16pub_type
杂志文章相关文献
PLACENTA文献大全abstract::We tested the hypothesis that the expression of placental connective tissue growth factor (CTGF) is enhanced in pregnancies complicated by severe preeclampsia (PE) or fetal growth restriction (FGR). CTGF expression was analyzed using immunostaining, western blot and real-time quantitative PCR in placental samples obta...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2009.08.006
更新日期:2009-11-01 00:00:00
abstract::Recent studies have illustrated the importance of placental drug transport proteins, such as P-glycoprotein (Pgp) and breast cancer resistance protein (BCRP) in limiting fetal exposure to drugs and toxins. Moreover, increasing evidence supports a role for Pgp and BCRP in the normal development and physiological functi...
journal_title:Placenta
pub_type: 杂志文章,评审
doi:10.1016/j.placenta.2010.02.010
更新日期:2010-05-01 00:00:00
abstract::Homeodomain (HDM) proteins encoded by homeobox (HBX) genes represent a large family of transcriptional factors that control differentiation and development in certain cell types. DLX4 is a member of Distal-less (DLX) family of HBX genes. Recent studies have demonstrated that abnormal expression of DLX4 is present in s...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2005.03.007
更新日期:2006-04-01 00:00:00
abstract::Pregnancy-associated plasma protein-A was assayed in the blood of 347 women during pregnancy, using a new primary standard of PAPP-A as reference. The protein was assayed by antibody-antigen crossed electrophoresis with the lower limit of confident assay being 9.5 micrograms PAPP-A/ml (13 pmol/ml). PAPP-A was first de...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/s0143-4004(82)80020-9
更新日期:1982-01-01 00:00:00
abstract:OBJECTIVE:To evaluate umbilical cord abnormalities predisposing to mechanical cord compression and determine their relationship to adverse clinical outcomes and stasis-associated histologic changes in the placenta. METHODS:Placental slides of 224 singleton pregnancies with gross cord abnormality (true knots, long cord...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2009.09.005
更新日期:2009-12-01 00:00:00
abstract::Maternal-to-fetal transmission of the murine lactate dehydrogenase-elevating virus (LDV) has been previously shown to be regulated by maternal immunity as well as gestational age. For the present study, the role of maternal immunity in placental and umbilical cord virus protection was studied, and virus targeting of u...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1053/plac.2002.0829
更新日期:2002-05-01 00:00:00
abstract::Placental development is a highly regulated process requiring signals from both fetal and maternal uterine compartments. Within this complex system, trophoblasts, placental cells of epithelial lineage, form the maternal-fetal interface controlling nutrient, gas and waste exchange. The commitment of progenitor villous ...
journal_title:Placenta
pub_type: 杂志文章,评审
doi:10.1016/j.placenta.2014.12.016
更新日期:2015-04-01 00:00:00
abstract::The ability to directly monitor the status of the placenta throughout pregnancy would be a major advance in both general and personalized obstetric care, allowing treatments to be tailored to the dynamic changes that can occur in gestation. Syncytiotrophoblast extracellular vesicles (STBEV) are membrane bound vesicles...
journal_title:Placenta
pub_type: 杂志文章,评审
doi:10.1016/j.placenta.2016.11.008
更新日期:2017-04-01 00:00:00
abstract::Long-chain polyunsaturated fatty acids (LCPUFAs) such as docosahexaenoic acid (DHA) and arachidonic acid (AA) are essential for proper development of fetal brain and retina. These LCPUFAs are selectively enriched in the fetal circulation compared with the maternal circulation. In the current study we investigated the ...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2008.10.007
更新日期:2009-01-01 00:00:00
abstract::The walls of uterine spiral arteries are invaded by extravillous trophoblast cells and are thereby converted to the uteroplacental arteries of pregnancy. The mechanisms by which this invasion occurs are not understood, but the extracellular matrices that are breached suggest participation by specific proteinases. In t...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/s0143-4004(05)80454-0
更新日期:1994-02-01 00:00:00
abstract::Although secretory phospholipase A2 (PLA2) isozymes have been identified in human gestational tissues, their role in homeostasis and pathophysiology during pregnancy has yet to be clearly established. The aims of this brief commentary are: (1) to review recent data concerning the expression of secretory PLA2 isozymes ...
journal_title:Placenta
pub_type: 杂志文章,评审
doi:10.1016/s0143-4004(98)90093-5
更新日期:1998-01-01 00:00:00
abstract::The sites of localization of luteinizing hormone (LH), follicle-stimulating hormone (FSH), growth hormone (GH), adrenocorticotrophic hormone (ACTH) and prolactin (PRL) within placental tissues have been studied by an immunoperoxidase technique. The syncytiotrophoblast is the sole significant site of localization of LH...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/s0143-4004(86)80007-8
更新日期:1986-03-01 00:00:00
abstract::Second trimester maternal serum human chorionic gonadotrophin (hCG) levels in women who remained normotensive but delivered an unexplained growth retarded infant were compared with those from a control group and a group of women who developed pre-eclampsia in a retrospective observational study. Our hypothesis was tha...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/s0143-4004(98)90002-9
更新日期:1998-03-01 00:00:00
abstract::The placenta is an abundant source of mesenchymal stem/stromal cells (MSC). Although presumed of translationally-advantageous fetal origin, the literature instead suggests a high incidence of either contaminating or pure maternal MSC. Despite definitional criteria that MSC are CD34-, increasing evidence suggests that ...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2014.09.001
更新日期:2014-11-01 00:00:00
abstract::Maternal fever is common during pregnancy and has for many years been suspected to harm the developing fetus. Whether increased maternal temperature produces exaggerated apoptosis in trophoblast cells remains unclear. Since p53 is a critical regulator of apoptosis we hypothesized that increased temperature in placenta...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2016.03.009
更新日期:2016-06-01 00:00:00
abstract::Placenta accreta is a pregnancy complication characterized by the presence of life-threatening uteroplacental neovascularization. The factors involving its development are unknown. Vascular endothelial growth factor (VEGF), placenta growth factor (PlGF) and their receptors (VEGFR) have important roles in vascular remo...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2004.12.011
更新日期:2006-01-01 00:00:00
abstract::Direct measurements of electrical potential and input resistance were performed on the syncytial membrane facing maternal blood. The potentials varied according to the external potassium ion levels and, to a lesser extent, with sodium ion concentrations. Ouabain provoked a rapid and consistent depolarization which ind...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/0143-4004(88)90012-4
更新日期:1988-03-01 00:00:00
abstract::The visceral yolk sac (VYS) is responsible for a major part of the amino acid nutrition of the early post-implantation rat embryo and possibly also at the fetal stage of gestation. The mechanism involves endocytic uptake of proteins by the tissue's epithelial cells followed by intralysosomal digestion to amino acids. ...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/0143-4004(77)90009-1
更新日期:1997-09-01 00:00:00
abstract::In view of the pathological placental features of pre-eclampsia and intrauterine growth restriction (IUGR), and the angiogenic effects of vascular endothelial growth factor (VEGF), the aim of this study was to determine the expression of VEGF in placentae from normal pregnancies and to compare the results with placent...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/s0143-4004(97)80061-6
更新日期:1997-05-01 00:00:00
abstract::Glutamine is the most versatile amino acid and its plasma concentration is the highest of all amino acid. Many transporters are therefore involved in glutamine uptake or efflux. Glutamine is actively released from the placenta into fetal circulation. In this study, we examined the alteration of transporters that trans...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2009.09.009
更新日期:2009-12-01 00:00:00
abstract:INTRODUCTION:The state of activation of dendritic cells (DCs) at the feto-maternal interface critically contributes to optimal decidual immune responses needed to support fetal-placental development. We recently demonstrated that during healthy pregnancy also peripheral blood DCs (PBDCs), which are easily accessible, a...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2012.10.016
更新日期:2013-01-01 00:00:00
abstract::Glycogen storage disease type IV (GSD IV) is a rare autosomal recessive disorder caused by glycogen branching enzyme (GBE) deficiency and resulting in the storage of abnormal glycogen (polyglucosan). Prenatal diagnosis is based on biochemical assay of GBE activity or on mutation analysis, but polyglucosan can also be ...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2008.01.005
更新日期:2008-04-01 00:00:00
abstract:BACKGROUND:Discrepancies in the results from studies of early events in human trophoblast invasion of decidua have been acknowledged and are attributed largely to deficiencies in the accuracy of sampling of the decidual tissue used in the research. We describe a novel technique of biopsy of decidua parietalis and basal...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2012.02.018
更新日期:2012-06-01 00:00:00
abstract::The molecular heterogeneity of pregnancy-specific beta 1-glycoprotein (SP1) was examined by analytical immunoelectrophoresis and a radioimmunostaining technique of immunoelectrophoretic plates. Analytical crossed immunoelectrophoretic analysis and radioimmunostaining of these plates demonstrated the presence of normal...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/s0143-4004(81)80006-9
更新日期:1981-07-01 00:00:00
abstract::Placental villi of early and term human placentae were dissociated with trypsin and mixed cell cultures were established. The different cell types were identified and estimated over a 14-day culture period using antibodies to keratin and vimentin filaments and their capacity to phagocytose yeast. The three main cell t...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/s0143-4004(84)80047-8
更新日期:1984-01-01 00:00:00
abstract:OBJECTIVE:To study the association between velamentous cord insertion (VCI) and different outcomes in monochorionic twins with and without twin-twin transfusion syndrome (TTTS). METHODS:We recorded the cord insertion type in all consecutive monochorionic placentas examined in two tertiary medical centers. The associat...
journal_title:Placenta
pub_type: 杂志文章,多中心研究
doi:10.1016/j.placenta.2013.08.009
更新日期:2013-11-01 00:00:00
abstract::A rapid method to determine quality for placental proteomic studies is required due to varying lengths of time between delivery and sampling in routine protocols. We developed a rapid 10 min LC-MS based scanning method to profile free peptides liberated from natural proteolytic degradation. The assay was applied to pl...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2016.04.014
更新日期:2016-08-01 00:00:00
abstract:INTRODUCTION:The aim of this study is to determine whether the gene expression and associated DNA methylation regulation of H19 and IGF2 are altered in placentas conceived by assisted reproductive technologies (ART) compared to natural conceptions. METHODS:113 pregnancies were recruited resulting in 119 placentas (83 ...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2015.08.008
更新日期:2015-10-01 00:00:00
abstract:INTRODUCTION:The most adverse perinatal outcome of intrahepatic cholestasis of pregnancy (ICP) is sudden fetal death related to acute fetoplacental hypoxia. Corticotrophin-releasing hormone (CRH), urocortin (UCN), and Wolfram syndrome 1 (WFS1) proteins may have a compensatory response to hypoxic stress. METHODS:A tota...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2019.06.378
更新日期:2019-08-01 00:00:00
abstract::Human amniotic epithelium from patients with normal pregnancy (normal amniotic epithelium) and from patients with materno-fetal rhesus incompatibility has been examined under the light and electron microscope. In comparison with normal amniotic epithelium, the epithelium in rhesus incompatibility displays the followin...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/s0143-4004(81)80038-0
更新日期:1981-01-01 00:00:00