Enzyme-linked immunosorbent assay for detection of antibodies to the unmodified beta-lactam ring.

Abstract:

:Dose- and pH- dependent carbodiimide-mediated coupling of Penicillin-G to polystyrene microtiter-plates that leaves the beta-lactam ring unchanged is described. A new ELISA method was developed using Penicillin-G coated plates. The binding of 3 different monoclonal antibodies as well as human IgG antibodies of the IgG1 and IgG3 subclasses is demonstrated, whereas IgG2, IgG4 and IgE antibodies did not bind. Thus, covalently coupled Penicillin-G can be used to study the immune-response to the unchanged beta-lactam ring in patients receiving penicillin therapy. The new method is complementary to hitherto described techniques, which generally only allow detection of antibodies binding to penicilloyl-groups.

journal_name

Allergy

journal_title

Allergy

authors

Klein GF,Stanzl U,Fritsch PO,Varga JM

doi

10.1111/j.1398-9995.1993.tb00705.x

subject

Has Abstract

pub_date

1993-04-01 00:00:00

pages

151-7

issue

3

eissn

0105-4538

issn

1398-9995

journal_volume

48

pub_type

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