Effect of an aromatase inhibitor in human follicular fluid on DNA synthesis of granulosa cells, theca cells, and follicles.

Abstract:

:An aromatase inhibitor was identified in human follicular fluid. We sought to examine the effects of this inhibitor on granulosa cells, theca cells, a mixture of granulosa and theca cells, and follicles. Granulosa cells, theca cells, the mixture of granulosa and theca cells, and follicles were incubated with [3H]-thymidine and aromatase inhibitor at 37 degrees C for 40 h in Ham F-10 medium. The radioactivities of acid-soluble fractions were counted. Aromatase inhibitor markedly inhibited the DNA synthesis of granulosa cells and follicles, but it did not inhibit the mixture of granulosa and theca cells. This suggested that aromatase inhibitor was a factor in the follicle paracrine system and acted mainly on granulosa cells.

journal_name

Gynecol Obstet Invest

authors

Ono T,Komiya Y,Kawagoe SN,Hiroi M

doi

10.1159/000292692

subject

Has Abstract

pub_date

1993-01-01 00:00:00

pages

169-71

issue

3

eissn

0378-7346

issn

1423-002X

journal_volume

35

pub_type

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