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Development of tissue damage, inflammation and resolution following stroke: an immunohistochemical and quantitative planimetric study.

Abstract：

:Development and resolution of the lesion produced by occlusion of the middle cerebral artery (MCAO) was studied through quantitative planimetry and histologic/immunohistochemical techniques. MCAO, performed in spontaneously hypertensive rats (SHR), initially (1-3 days) produced large, consistent cerebral cortical infarctions and an increase in ipsilateral hemispheric size (i.e., swelling) quantitated by planimetry on 2,3,5-triphenyltetrazolium chloride (TTC)-stained gross tissue sections. These initial changes correlated well with changes identified from 2 h to 3 days using hematoxylin and eosin stained histologic tissue sections and immunohistochemical techniques including: the progressive development of a cortical area of pan necrosis, infiltration of neutrophils into infarcted tissues, and activation of astroglia. During the initial 2 days following MCAO, glial fibrillary acidic protein immunoreactive cells increased in number and became larger and more intensely fluorescent medial to the cortical infarct. At 5 to 15 days, both the infarct and the ipsilateral hemisphere decreased in size. These changes correlated with the presence of abundant macrophages, and cavitation of the lesion along its medial border. Also during this period, a loose connective tissue matrix formed along the superficial aspect of the infarct. This connective tissue contained fibroblasts, extracellular matrix immunoreactive for laminin and collagen, capillary buds indicating neovascularization, and abundant macrophages. By the final timepoint (30 days), necrotic tissue could no longer be detected in either gross or histologic tissue sections, the inflammatory infiltrate had resolved, and the connective tissue was removed.(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

Brain Res Bull

journal_title

Brain research bulletin

authors

Clark RK,Lee EV,Fish CJ,White RF,Price WJ,Jonak ZL,Feuerstein GZ,Barone FC

doi

10.1016/0361-9230(93)90124-t

subject

Has Abstract

pub_date

1993-01-01 00:00:00

pages

565-72

issue

eissn

0361-9230

issn

1873-2747

pii

0361-9230(93)90124-T

journal_volume

pub_type

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Development of tissue damage, inflammation and resolution following stroke: an immunohistochemical and quantitative planimetric study.