当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > An analogue of the DnaJ molecular chaperone in Escherichia coli.

An analogue of the DnaJ molecular chaperone in Escherichia coli.

Abstract：

:Escherichia coli DnaJ functions as a typical molecular chaperone in coordination with other heat shock proteins such as DnaK and GrpE in a variety of cellular processes. In this study, it was found that E. coli possesses an analogue of DnaJ, as judged from not only its primary structure but also its possible function. This protein, named CbpA (for curved DNA-binding protein), was first identified as a DNA-binding protein that preferentially recognizes a curved DNA sequence. Cloning and nucleotide sequencing of the gene encoding CbpA revealed that the predicted product is very similar to DnaJ in amino acid sequence: overall identity is 39%. The cbpA gene functions as a multicopy suppressor for dnaJ mutations. The mutational lesions characteristic of a dnaJ null mutant--namely, temperature sensitivity for growth and defects in lambda phage and mini-F DNA replication--were all restored upon introduction of the cbpA gene on a multicopy plasmid. An insertional mutant of cbpA was also isolated, which showed no noticeable phenotype, particularly with regard to temperature sensitivity for growth. However, when this cbpA::kan allele was combined with the dnaJ null allele, the resultant strain was unable to grow at 37 degrees C, at which strains carrying each mutation alone could grow normally. These genetic results are interpreted as meaning that the function(s) of CbpA in E. coli is closely related to that of DnaJ.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Ueguchi C,Kakeda M,Yamada H,Mizuno T

doi

10.1073/pnas.91.3.1054

subject

Has Abstract

pub_date

1994-02-01 00:00:00

pages

1054-8

issue

3

eissn

0027-8424

issn

1091-6490

journal_volume

91

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • The wound repair response controls outcome to cutaneous leishmaniasis.

    abstract：:Chronic microbial infections are associated with fibrotic and inflammatory reactions known as granulomas showing similarities to wound-healing and tissue repair processes. We have previously mapped three leishmaniasis susceptibility loci, designated lmr1, -2, and -3, which exert their effect independently of T cell im...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0505630102

    authors： Sakthianandeswaren A,Elso CM,Simpson K,Curtis JM,Kumar B,Speed TP,Handman E,Foote SJ

    更新日期：2005-10-25 00:00:00

  • Structural and functional conservation of two human homologs of the yeast DNA repair gene RAD6.

    abstract：:The RAD6 gene of Saccharomyces cerevisiae encodes a ubiquitin-conjugating enzyme (E2) that is required for DNA repair, damage-induced mutagenesis, and sporulation. We have cloned the two human RAD6 homologs, designated HHR6A and HHR6B. The two 152-amino acid human proteins share 95% sequence identity with each other a...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.88.20.8865

    authors： Koken MH,Reynolds P,Jaspers-Dekker I,Prakash L,Prakash S,Bootsma D,Hoeijmakers JH

    更新日期：1991-10-15 00:00:00

  • Identification and functional activity of prolactin receptors in thymic epithelial cells.

    abstract：:Thymic epithelial cells (TECs), the major component of the thymic microenvironment, can be modulated by pituitary hormones. We have shown previously that prolactin (PRL) can influence the endocrine activity of TECs and stimulate TEC proliferation as well as cytokeratin expression, suggesting the existence of PRL recep...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.88.21.9700

    authors： Dardenne M,Kelly PA,Bach JF,Savino W

    更新日期：1991-11-01 00:00:00

  • Mechanisms determining aerobic or anaerobic growth in the facultative anaerobe Salmonella typhimurium.

    abstract：:We isolated mutant strains of the facultative anaerobe Salmonella typhimurium that grow either aerobically or anaerobically. Strict anaerobic mutants contained a defective DNA topoisomerase I gene (topI), while strict aerobic mutants contained a defective DNA gyrase subunit A gene (gyrA, also nalA). Topoisomerase I ac...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.82.7.2077

    authors： Yamamoto N,Droffner ML

    更新日期：1985-04-01 00:00:00

  • Pack-Mutator-like transposable elements (Pack-MULEs) induce directional modification of genes through biased insertion and DNA acquisition.

    abstract：:In monocots, many genes demonstrate a significant negative GC gradient, meaning that the GC content declines along the orientation of transcription. Such a gradient is not observed in the genes of the dicot plant Arabidopsis. In addition, a lack of homology is often observed when comparing the 5' end of the coding reg...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1010814108

    authors： Jiang N,Ferguson AA,Slotkin RK,Lisch D

    更新日期：2011-01-25 00:00:00

  • rRNA complementarity within mRNAs: a possible basis for mRNA-ribosome interactions and translational control.

    abstract：:Our recent demonstration that many eukaryotic mRNAs contain sequences complementary to rRNA led to the hypothesis that these sequences might mediate specific interactions between mRNAs and ribosomes and thereby affect translation. In the present experiments, the ability of complementary sequences to bind to rRNA was i...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.21.12238

    authors： Tranque P,Hu MC,Edelman GM,Mauro VP

    更新日期：1998-10-13 00:00:00

  • Arginine methylation is required for canonical Wnt signaling and endolysosomal trafficking.

    abstract：:Arginine methylation has emerged as a widespread and reversible protein modification with the potential to regulate a multitude of cellular processes, but its function is poorly understood. Endolysosomes play an important role in Wnt signaling, in which glycogen synthase kinase 3 (GSK3) becomes sequestered inside mult...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1804091115

    authors： Albrecht LV,Ploper D,Tejeda-Muñoz N,De Robertis EM

    更新日期：2018-06-05 00:00:00

  • Translocation of phospholipids from the inner to the outer membrane of Escherichia coli.

    abstract：:The translocation of lipids from the inner to the outer membrane of Escherichia coli has been investigated by pulse-chase experiments. After a pulse with [2-3H]glycerol, the specific activity of the newly synthesized [3H]phosphatidylethanolamine was 5 times greater in the inner than in the outer membrane. During the c...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.77.4.1867

    authors： Donohue-Rolfe AM,Schaechter M

    更新日期：1980-04-01 00:00:00

  • Probing the active site tightness of DNA polymerase in subangstrom increments.

    abstract：:We describe the use of a series of gradually expanded thymine nucleobase analogs in probing steric effects in DNA polymerase efficiency and fidelity. In these nonpolar compounds, the base size was increased incrementally over a 1.0-A range by use of variably sized atoms (H, F, Cl, Br, and I) to replace the oxygen mole...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0505113102

    authors： Kim TW,Delaney JC,Essigmann JM,Kool ET

    更新日期：2005-11-01 00:00:00

  • Lignin content in natural Populus variants affects sugar release.

    abstract：:The primary obstacle to producing renewable fuels from lignocellulosic biomass is a plant's recalcitrance to releasing sugars bound in the cell wall. From a sample set of wood cores representing 1,100 individual undomesticated Populus trichocarpa trees, 47 extreme phenotypes were selected across measured lignin conten...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1009252108

    authors： Studer MH,DeMartini JD,Davis MF,Sykes RW,Davison B,Keller M,Tuskan GA,Wyman CE

    更新日期：2011-04-12 00:00:00

  • 31P nuclear magnetic relaxation studies of phosphocreatine in intact muscle: determination of intracellular free magnesium.

    abstract：:31P nuclear magnetic relaxation rates for phosphocreatine in intact frog gastrocnemius were compared with those observed in model solutions at 4 degrees, a temperature at which muscle maintains its physiological state for at least 5 hr. Both nuclear Overhauser effect and spin-lattice relaxation rate (1/T1) experiments...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.74.10.4271

    authors： Cohen SM,Burt CT

    更新日期：1977-10-01 00:00:00

  • Metallothioneins negatively regulate IL-27-induced type 1 regulatory T-cell differentiation.

    abstract：:IL-27-induced type 1 regulatory T (Tr1) cells suppress autoimmunity by producing IL-10. Signal transducer and activator of transcription (STAT) 1 and STAT3 have been described as key transcription factors that promote IL-10 secretion from Tr1 cells induced by IL-27. However, the molecular pathways for negatively regul...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1211776110

    authors： Wu C,Pot C,Apetoh L,Thalhamer T,Zhu B,Murugaiyan G,Xiao S,Lee Y,Rangachari M,Yosef N,Kuchroo VK

    更新日期：2013-05-07 00:00:00

  • The leukemic fusion gene AML1-MDS1-EVI1 suppresses CEBPA in acute myeloid leukemia by activation of Calreticulin.

    abstract：:The leukemic fusion gene AML1-MDS1-EVI1 (AME) encodes a chimeric transcription factor that results from the t(3,21)(q26;q22) translocation seen in patients with acute myeloid leukemia, with therapy-related myelodysplastic syndrome, or with chronic myeloid leukemia in blast crisis. The myeloid transcription factor CEBP...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0404731101

    authors： Helbling D,Mueller BU,Timchenko NA,Hagemeijer A,Jotterand M,Meyer-Monard S,Lister A,Rowley JD,Huegli B,Fey MF,Pabst T

    更新日期：2004-09-07 00:00:00

  • Crystal structure and mechanistic determinants of SARS coronavirus nonstructural protein 15 define an endoribonuclease family.

    abstract：:The approximately 30-kb coronavirus (+)RNA genome is replicated and transcribed by a membrane-bound replicase complex made up of 16 viral nonstructural proteins (nsp) with multiple enzymatic activities. The complex includes an RNA endonuclease, NendoU, that is conserved among nidoviruses but no other RNA virus, making...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0601708103

    authors： Ricagno S,Egloff MP,Ulferts R,Coutard B,Nurizzo D,Campanacci V,Cambillau C,Ziebuhr J,Canard B

    更新日期：2006-08-08 00:00:00

  • Library preparation methodology can influence genomic and functional predictions in human microbiome research.

    abstract：:Observations from human microbiome studies are often conflicting or inconclusive. Many factors likely contribute to these issues including small cohort sizes, sample collection, and handling and processing differences. The field of microbiome research is moving from 16S rDNA gene sequencing to a more comprehensive gen...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1519288112

    authors： Jones MB,Highlander SK,Anderson EL,Li W,Dayrit M,Klitgord N,Fabani MM,Seguritan V,Green J,Pride DT,Yooseph S,Biggs W,Nelson KE,Venter JC

    更新日期：2015-11-10 00:00:00

  • The silencing protein SIR2 and its homologs are NAD-dependent protein deacetylases.

    abstract：:Homologs of the chromatin-bound yeast silent information regulator 2 (SIR2) protein are found in organisms from all biological kingdoms. SIR2 itself was originally discovered to influence mating-type control in haploid cells by locus-specific transcriptional silencing. Since then, SIR2 and its homologs have been sugge...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.110148297

    authors： Landry J,Sutton A,Tafrov ST,Heller RC,Stebbins J,Pillus L,Sternglanz R

    更新日期：2000-05-23 00:00:00

  • Neuroinflammation triggered by β-glucan/dectin-1 signaling enables CNS axon regeneration.

    abstract：:Innate immunity can facilitate nervous system regeneration, yet the underlying cellular and molecular mechanisms are not well understood. Here we show that intraocular injection of lipopolysaccharide (LPS), a bacterial cell wall component, or the fungal cell wall extract zymosan both lead to rapid and comparable intra...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1423221112

    authors： Baldwin KT,Carbajal KS,Segal BM,Giger RJ

    更新日期：2015-02-24 00:00:00

  • Abnormal junctions between surface membrane and sarcoplasmic reticulum in skeletal muscle with a mutation targeted to the ryanodine receptor.

    abstract：:Junctions that mediate excitation-contraction (e-c) coupling are formed between the sarcoplasmic reticulum (SR) and either the surface membrane or the transverse (T) tubules in normal skeletal muscle. Two structural components of the junctions, the feet of the SR and the tetrads of T tubules, have been identified resp...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.8.3381

    authors： Takekura H,Nishi M,Noda T,Takeshima H,Franzini-Armstrong C

    更新日期：1995-04-11 00:00:00

  • A soluble domain of the membrane-anchoring chain of influenza virus hemagglutinin (HA2) folds in Escherichia coli into the low-pH-induced conformation.

    abstract：:The extensive refolding of the membrane-anchoring chain of hemagglutinin (HA) of influenza virus (termed HA2) in cellular endosomes, which initiates viral entry by membrane fusion, suggests that viral HA is meta-stable. HA2 polypeptide residues 38-175 expressed in Escherichia coli are reported here to fold in vivo int...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.26.12205

    authors： Chen J,Wharton SA,Weissenhorn W,Calder LJ,Hughson FM,Skehel JJ,Wiley DC

    更新日期：1995-12-19 00:00:00

  • RNF169 limits 53BP1 deposition at DSBs to stimulate single-strand annealing repair.

    abstract：:Unrestrained 53BP1 activity at DNA double-strand breaks (DSBs) hampers DNA end resection and upsets DSB repair pathway choice. RNF169 acts as a molecular rheostat to limit 53BP1 deposition at DSBs, but how this fine balance translates to DSB repair control remains undefined. In striking contrast to 53BP1, ChIP analyse...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1804823115

    authors： An L,Dong C,Li J,Chen J,Yuan J,Huang J,Chan KM,Yu CH,Huen MSY

    更新日期：2018-08-28 00:00:00

  • Protein D1 preferentially binds A + T-rich DNA in vitro and is a component of Drosophila melanogaster nucleosomes containing A + T-rich satellite DNA.

    abstract：:Our previous work [Levinger, L. & Varshavsky, A. (1982) Cell 28, 375-385] has shown that D1, a 50-kilodalton chromosomal protein of Drosophila melanogaster, is specifically associated with isolated nucleosomes that contain a complex A + T-rich satellite DNA with buoyant density of 1.688 g/ml. We show here that D1 is a...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.79.23.7152

    authors： Levinger L,Varshavsky A

    更新日期：1982-12-01 00:00:00

  • Proteins conferred by the virulence-specifying plasmid of Agrobacterium tumefaciens C-58.

    abstract：:Membrane-associated and periplasmic proteins of Agrobacterium tumefaciens C-58 were compared with those from avirulent (nontumrigenic) derivative strains by slab and two-dimensional gel electrophoresis. Two proteins (Per-I and Per-2), with a molecular weight of 37,500 and 37,300, respectively, were detected in the sup...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.75.8.3796

    authors： Sonoki S,Kado CI

    更新日期：1978-08-01 00:00:00

  • Plasma radioiron kinetics in man: explanation for the effect of plasma iron concentration.

    abstract：:The plasma iron turnover was measured in 19 normal subjects. A correlation was found between plasma iron concentration and plasma iron turnover. In addition to the turnover of 55Fe at normal plasma iron concentration (predominantly monoferric transferrin), a second turnover in which the labeled plasma was saturated wi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.75.3.1559

    authors： Skarberg K,Eng M,Huebers H,Marsaglia G,Finch C

    更新日期：1978-03-01 00:00:00

  • Facial color is an efficient mechanism to visually transmit emotion.

    abstract：:Facial expressions of emotion in humans are believed to be produced by contracting one's facial muscles, generally called action units. However, the surface of the face is also innervated with a large network of blood vessels. Blood flow variations in these vessels yield visible color changes on the face. Here, we stu...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1716084115

    authors： Benitez-Quiroz CF,Srinivasan R,Martinez AM

    更新日期：2018-04-03 00:00:00

  • Endonuclease S1-sensitive site in chicken pro-alpha 2(I) collagen 5' flanking gene region.

    abstract：:A site that is preferentially cleaved by the single-strand-specific endonuclease from Aspergillus oryzae was located in vitro 180 base pairs upstream from the 5' end of the chicken pro-alpha 2(I) collagen gene. It is found in supercoiled plasmids with a negative superhelical density of -0.024 or more but not in linear...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.81.6.1659

    authors： Finer MH,Fodor EJ,Boedtker H,Doty P

    更新日期：1984-03-01 00:00:00

  • Distinct quaternary structures of the AAA+ Lon protease control substrate degradation.

    abstract：:Lon is an ATPase associated with cellular activities (AAA+) protease that controls cell division in response to stress and also degrades misfolded and damaged proteins. Subunits of Lon are known to assemble into ring-shaped homohexamers that enclose an internal degradation chamber. Here, we demonstrate that hexamers o...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1307066110

    authors： Vieux EF,Wohlever ML,Chen JZ,Sauer RT,Baker TA

    更新日期：2013-05-28 00:00:00

  • Fluctuation broadening in carbon nanotube resonators.

    abstract：:We simulated the behavior of suspended carbon nanotube resonators over a broad range of temperatures to explore the physics of semiflexible polymers in underdamped environments. We find that thermal fluctuations induce strong coupling between resonance modes. This effect leads to spectral fluctuations that readily acc...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1216407109

    authors： Barnard AW,Sazonova V,van der Zande AM,McEuen PL

    更新日期：2012-11-20 00:00:00

  • Antidepressant-relevant concentrations of the ketamine metabolite (2R,6R)-hydroxynorketamine do not block NMDA receptor function.

    abstract：:Preclinical studies indicate that (2R,6R)-hydroxynorketamine (HNK) is a putative fast-acting antidepressant candidate. Although inhibition of NMDA-type glutamate receptors (NMDARs) is one mechanism proposed to underlie ketamine's antidepressant and adverse effects, the potency of (2R,6R)-HNK to inhibit NMDARs has not ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1816071116

    authors： Lumsden EW,Troppoli TA,Myers SJ,Zanos P,Aracava Y,Kehr J,Lovett J,Kim S,Wang FH,Schmidt S,Jenne CE,Yuan P,Morris PJ,Thomas CJ,Zarate CA Jr,Moaddel R,Traynelis SF,Pereira EFR,Thompson SM,Albuquerque EX,Gould TD

    更新日期：2019-03-12 00:00:00

  • Binding of histones H1 and H5 and their globular domains to four-way junction DNA.

    abstract：:We have compared chicken erythrocyte linker histones H1 and H5 binding to a synthetic four-way DNA junction. Each histone binds to form a single complex, with an affinity which permits competition against a large excess of linear duplex DNA. The affinity of H5 is higher than that of H1. The globular domain from either...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.9.3525

    authors： Varga-Weisz P,Zlatanova J,Leuba SH,Schroth GP,van Holde K

    更新日期：1994-04-26 00:00:00

  • GABAB receptor deficiency causes failure of neuronal homeostasis in hippocampal networks.

    abstract：:Stabilization of neuronal activity by homeostatic control systems is fundamental for proper functioning of neural circuits. Failure in neuronal homeostasis has been hypothesized to underlie common pathophysiological mechanisms in a variety of brain disorders. However, the key molecules regulating homeostasis in centra...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1424810112

    authors： Vertkin I,Styr B,Slomowitz E,Ofir N,Shapira I,Berner D,Fedorova T,Laviv T,Barak-Broner N,Greitzer-Antes D,Gassmann M,Bettler B,Lotan I,Slutsky I

    更新日期：2015-06-23 00:00:00