A soluble domain of the membrane-anchoring chain of influenza virus hemagglutinin (HA2) folds in Escherichia coli into the low-pH-induced conformation.

Abstract:

:The extensive refolding of the membrane-anchoring chain of hemagglutinin (HA) of influenza virus (termed HA2) in cellular endosomes, which initiates viral entry by membrane fusion, suggests that viral HA is meta-stable. HA2 polypeptide residues 38-175 expressed in Escherichia coli are reported here to fold in vivo into a soluble trimer. The structure appears to be the same as the low-pH-induced conformation of viral HA2 by alpha-helical content, thermodynamic stability, protease dissection, electron microscopy, and antibody binding. These results provide evidence that the structure of the low-pH-induced fold of viral HA2 (TBHA2) observed crystallographically is the lowest-energy-state fold of the HA2 polypeptide. They indicate that the HA2 conformation in viral HA before low pH activation of its fusion potential is metastable and suggest that removal of the receptor-binding chain (HA1) is enough to allow HA2 to adopt the stable state. Further, they provide direct evidence that low pH is not required to form the membrane-fusion conformation but acts to make this state kinetically accessible in viral HA.

authors

Chen J,Wharton SA,Weissenhorn W,Calder LJ,Hughson FM,Skehel JJ,Wiley DC

doi

10.1073/pnas.92.26.12205

subject

Has Abstract

pub_date

1995-12-19 00:00:00

pages

12205-9

issue

26

eissn

0027-8424

issn

1091-6490

journal_volume

92

pub_type

杂志文章
  • Polyploid megakaryocytes develop randomly from a multicompartmental system of committed progenitors.

    abstract::Cumulative distributions of the number of doublings undergone by mixed megakaryocytic/erythroblastic colonies and by pure megakaryocytic colonies were determined from plasma clot cultures of bone marrow (from C57BL/6 mice) supplemented with erythropoietin. Analysis of these distributions suggests that these colonies a...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.79.14.4410

    authors: Paulus JM,Prenant M,Deschamps JF,Henry-Amar M

    更新日期:1982-07-01 00:00:00

  • The endoplasmic reticulum-sarcoplasmic reticulum connection: distribution of endoplasmic reticulum markers in the sarcoplasmic reticulum of skeletal muscle fibers.

    abstract::The skeletal muscle sarcoplasmic reticulum (SR) was investigated for the presence of well-known endoplasmic reticulum (ER) markers: the lumenal protein BiP and a group of membrane proteins recognized by an antibody raised against ER membrane vesicles. Western blots of SR fractions revealed the presence of BiP in fast-...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.89.13.6142

    authors: Volpe P,Villa A,Podini P,Martini A,Nori A,Panzeri MC,Meldolesi J

    更新日期:1992-07-01 00:00:00

  • Mouse model recapitulating human Fcγ receptor structural and functional diversity.

    abstract::The in vivo biological activities of IgG antibodies result from their bifunctional nature, in which antigen recognition by the Fab is coupled to the effector and immunomodulatory diversity found in the Fc domain. This diversity, resulting from both amino acid and glycan heterogeneity, is translated into cellular respo...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1203954109

    authors: Smith P,DiLillo DJ,Bournazos S,Li F,Ravetch JV

    更新日期:2012-04-17 00:00:00

  • Nef increases the synthesis of and transports cholesterol to lipid rafts and HIV-1 progeny virions.

    abstract::HIV buds from lipid rafts and requires cholesterol for its egress from and entry into cells. Viral accessory protein Nef plays a major role in this process. In this study, it not only increased the biosynthesis of lipid rafts and viral particles with newly synthesized cholesterol, but also enriched them. Furthermore, ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1437453100

    authors: Zheng YH,Plemenitas A,Fielding CJ,Peterlin BM

    更新日期:2003-07-08 00:00:00

  • Stop codon in the procollagen II gene (COL2A1) in a family with the Stickler syndrome (arthro-ophthalmopathy).

    abstract::Linkage analysis with restriction fragment length polymorphisms for the gene for type II procollagen (COL2A1) was carried out in a family with the Stickler syndrome, or arthro-ophthalmopathy, an autosomal dominant disorder that affects the eyes, ears, joints, and skeleton. The analysis demonstrated linkage of the dise...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.88.15.6624

    authors: Ahmad NN,Ala-Kokko L,Knowlton RG,Jimenez SA,Weaver EJ,Maguire JI,Tasman W,Prockop DJ

    更新日期:1991-08-01 00:00:00

  • Circadian clock-controlled genes isolated from Neurospora crassa are late night- to early morning-specific.

    abstract::An endogenous circadian biological clock controls the temporal aspects of life in most organisms, including rhythmic control of genes involved in clock output pathways. In the fungus Neurospora crassa, one pathway known to be under control of the clock is asexual spore (conidia) development. To understand more fully t...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.93.23.13096

    authors: Bell-Pedersen D,Shinohara ML,Loros JJ,Dunlap JC

    更新日期:1996-11-12 00:00:00

  • Positional specificity of different transcription factor classes within enhancers.

    abstract::Gene expression is controlled by sequence-specific transcription factors (TFs), which bind to regulatory sequences in DNA. TF binding occurs in nucleosome-depleted regions of DNA (NDRs), which generally encompass regions with lengths similar to those protected by nucleosomes. However, less is known about where within ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1804663115

    authors: Grossman SR,Engreitz J,Ray JP,Nguyen TH,Hacohen N,Lander ES

    更新日期:2018-07-24 00:00:00

  • Predicting free choices for abstract intentions.

    abstract::Unconscious neural activity has been repeatedly shown to precede and potentially even influence subsequent free decisions. However, to date, such findings have been mostly restricted to simple motor choices, and despite considerable debate, there is no evidence that the outcome of more complex free decisions can be pr...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1212218110

    authors: Soon CS,He AH,Bode S,Haynes JD

    更新日期:2013-04-09 00:00:00

  • Predicting consumer behavior with Web search.

    abstract::Recent work has demonstrated that Web search volume can "predict the present," meaning that it can be used to accurately track outcomes such as unemployment levels, auto and home sales, and disease prevalence in near real time. Here we show that what consumers are searching for online can also predict their collective...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1005962107

    authors: Goel S,Hofman JM,Lahaie S,Pennock DM,Watts DJ

    更新日期:2010-10-12 00:00:00

  • NinaB combines carotenoid oxygenase and retinoid isomerase activity in a single polypeptide.

    abstract::In animals, successful production of the visual chromophore (11-cis-retinal or derivatives thereof such as 11-cis-3-hydroxy-retinal) is essential for photoreceptor cell function and survival. These carotenoid-derived compounds must combine with a protein moiety (the opsin) to establish functional visual pigments. Evid...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0807805105

    authors: Oberhauser V,Voolstra O,Bangert A,von Lintig J,Vogt K

    更新日期:2008-12-02 00:00:00

  • Interaction of the 82-kDa subunit of the vaccinia virus early transcription factor heterodimer with the promoter core sequence directs downstream DNA binding of the 70-kDa subunit.

    abstract::The vaccinia virus early transcription factor (VETF), a heterodimeric protein composed of 82- and 70-kDa subunits, interacts with viral early promoters at both a sequence-specific core region upstream and a sequence-independent region downstream of the RNA start site. To determine the VETF subunit-promoter interaction...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.93.15.7540

    authors: Cassetti MA,Moss B

    更新日期:1996-07-23 00:00:00

  • Manganese and zinc blockade of enzyme induction: studies with microsomal heme oxygenase.

    abstract::Heme oxygenase (decyclizing) [heme,hydrogen-donor:oxygen oxidoreductase (alpha-methene-oxidizing, hydroxylating), EC 1.14.99.3] is greatly induced in the kidney by the administration of nickel or tin. Manganese, when administered simultaneously with nickel or tin in an equimolar amount, substantially inhibited the ind...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.76.10.5331

    authors: Drummond GS,Kappas A

    更新日期:1979-10-01 00:00:00

  • Organic-mineral interfacial chemistry drives heterogeneous nucleation of Sr-rich (Ba x , Sr1-x )SO4 from undersaturated solution.

    abstract::Sr-bearing marine barite [(Ba x , Sr1-x )SO4] cycling has been widely used to reconstruct geochemical evolutions of paleoenvironments. However, an understanding of barite precipitation in the ocean, which is globally undersaturated with respect to barite, is missing. Moreo...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1821065116

    authors: Deng N,Stack AG,Weber J,Cao B,De Yoreo JJ,Hu Y

    更新日期:2019-07-02 00:00:00

  • Overexpression of an Aplysia shaker K+ channel gene modifies the electrical properties and synaptic efficacy of identified Aplysia neurons.

    abstract::Although potassium channels play a variety of roles in shaping the electrical properties of neurons, little is known about how these channels are constituted in neurons. To examine the assembly and physiological function of A-type K+ channels in mature differentiated neurons, we have developed a highly efficient gene ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.89.3.1133

    authors: Kaang BK,Pfaffinger PJ,Grant SG,Kandel ER,Furukawa Y

    更新日期:1992-02-01 00:00:00

  • Purification and initial characterization of a potential plant vacuolar targeting receptor.

    abstract::Clathrin-coated vesicles are known to be involved in the transport of proteins from the Golgi to the vacuole in plant cells. The mechanisms by which proteins are directed into this pathway are not known. Here we identify an integral membrane protein of approximately 80 kDa, extracted from clathrin-coated vesicles of d...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.91.8.3403

    authors: Kirsch T,Paris N,Butler JM,Beevers L,Rogers JC

    更新日期:1994-04-12 00:00:00

  • Biosynthesis and endocrine control of the production of the German cockroach sex pheromone 3,11-dimethylnonacosan-2-one.

    abstract::The biosynthesis and endocrine regulation of sex pheromone production in the female German cockroach (Blattella germanica) were determined. Radio-TLC and radio-GLC were used to demonstrate the metabolism of 3,11-dimethylnonacosane, a major cuticular lipid component, to the corresponding alkan-2-ol and methyl ketone. [...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.89.13.6050

    authors: Chase J,Touhara K,Prestwich GD,Schal C,Blomquist GJ

    更新日期:1992-07-01 00:00:00

  • Blockade of beta 1- but not of beta 2-adrenergic receptors replicates propranolol's suppression of the cerebral spread of an engram in mice.

    abstract::Bitemporal injections of puromycin that primarily affect the hippocampal-entorhinal area induce amnesia of aversive maze-learning in mice for 3 days after training but are ineffective 6 or more days after training. At these later times, additional puromycin sites covering widespread forebrain areas are necessary to in...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.82.21.7458

    authors: Flexner JB,Flexner LB,Church AC,Rainbow TC,Brunswick DJ

    更新日期:1985-11-01 00:00:00

  • Transposon-specified site-specific recombination.

    abstract::Cointegrate DNA molecules containing two copies of a transposable element appear to be intermediates in the transposition process. These structures are resolved by site-specific recombination to yield the normal end products of transposition. The transposable element gamma delta (Tn1000) synthesizes a product intercha...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.79.1.46

    authors: Kitts P,Symington L,Burke M,Reed R,Sherratt D

    更新日期:1982-01-01 00:00:00

  • Molecular cloning and sequence analysis of expansins--a highly conserved, multigene family of proteins that mediate cell wall extension in plants.

    abstract::Expansins are unusual proteins discovered by virtue of their ability to mediate cell wall extension in plants. We identified cDNA clones for two cucumber expansins on the basis of peptide sequences of proteins purified from cucumber hypocotyls. The expansin cDNAs encode related proteins with signal peptides predicted ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.92.20.9245

    authors: Shcherban TY,Shi J,Durachko DM,Guiltinan MJ,McQueen-Mason SJ,Shieh M,Cosgrove DJ

    更新日期:1995-09-26 00:00:00

  • Expression of cellular oncogenes in primary cells from human acute leukemias.

    abstract::The structure and the expression of 11 cellular oncogenes (protooncogenes) were analyzed in primary cells from 20 acute lymphocytic (ALL) and 31 acute myelogenous (AML) leukemia patients. Neoplastic cells, obtained prior to initiation of therapy, were purified and classified, on the basis of both surface antigen patte...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.83.12.4394

    authors: Mavilio F,Sposi NM,Petrini M,Bottero L,Marinucci M,De Rossi G,Amadori S,Mandelli F,Peschle C

    更新日期:1986-06-01 00:00:00

  • Cytokine-stimulated human immunodeficiency virus replication is inhibited by N-acetyl-L-cysteine.

    abstract::We show that the stimulation of human immunodeficiency virus (HIV) brought about by tumor necrosis factor alpha and phorbol 12-myristate 13-acetate can be inhibited by adding N-acetyl-L-cysteine (NAC). NAC, which replenishes intracellular glutathione, effectively inhibits the tumor necrosis factor alpha- or phorbol es...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.87.12.4884

    authors: Roederer M,Staal FJ,Raju PA,Ela SW,Herzenberg LA,Herzenberg LA

    更新日期:1990-06-01 00:00:00

  • New roles for cis-jasmone as an insect semiochemical and in plant defense.

    abstract::cis-jasmone, or (Z)-jasmone, is well known as a component of plant volatiles, and its release can be induced by damage, for example during insect herbivory. Using the olfactory system of the lettuce aphid to investigate volatiles from plants avoided by this insect, (Z)-jasmone was found to be electrophysiologically ac...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.160241697

    authors: Birkett MA,Campbell CA,Chamberlain K,Guerrieri E,Hick AJ,Martin JL,Matthes M,Napier JA,Pettersson J,Pickett JA,Poppy GM,Pow EM,Pye BJ,Smart LE,Wadhams GH,Wadhams LJ,Woodcock CM

    更新日期:2000-08-01 00:00:00

  • The cytoplasmic component of the bacterial flagellar motor.

    abstract::We have used electron microscopy to examine freshly isolated Salmonella typhimurium and Escherichia coli basal flagellar fragments, purified without resort to extremes of pH or ionic strength. Such fragments contain the large bell-like basal structures visualized recently in freeze-substituted or fixed preparations. W...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.89.13.5956

    authors: Khan IH,Reese TS,Khan S

    更新日期:1992-07-01 00:00:00

  • Insulin stimulates a membrane-bound serine kinase that may be phosphorylated on tyrosine.

    abstract::Triton X-100-solubilized high-density microsomes from insulin-treated rat adipocytes exhibit a marked increase in serine/threonine and tyrosine kinase activities toward exogenous histone when compared to controls. The insulin-dependent activation of microsomal histone kinase activities occurs within the physiological ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.84.12.3972

    authors: Yu KT,Khalaf N,Czech MP

    更新日期:1987-06-01 00:00:00

  • LRRK2 and its substrate Rab GTPases are sequentially targeted onto stressed lysosomes and maintain their homeostasis.

    abstract::Leucine-rich repeat kinase 2 (LRRK2) has been associated with a variety of human diseases, including Parkinson's disease and Crohn's disease, whereas LRRK2 deficiency leads to accumulation of abnormal lysosomes in aged animals. However, the cellular roles and mechanisms of LRRK2-mediated lysosomal regulation have rema...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1812196115

    authors: Eguchi T,Kuwahara T,Sakurai M,Komori T,Fujimoto T,Ito G,Yoshimura SI,Harada A,Fukuda M,Koike M,Iwatsubo T

    更新日期:2018-09-25 00:00:00

  • Autoimmune cardiomyopathy and heart block develop spontaneously in HLA-DQ8 transgenic IAbeta knockout NOD mice.

    abstract::A line of nonobese diabetic (NOD) mice expressing the human diabetes-associated HLA-DQ8 transgene in the absence of mouse IAbeta failed to show spontaneous insulitis or diabetes, but rather developed dilated cardiomyopathy, leading to early death from heart failure. Pathology in these animals results from an organ- an...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.2235552100

    authors: Elliott JF,Liu J,Yuan ZN,Bautista-Lopez N,Wallbank SL,Suzuki K,Rayner D,Nation P,Robertson MA,Liu G,Kavanagh KM

    更新日期:2003-11-11 00:00:00

  • Hemorrhage in lesions caused by cowpox virus is induced by a viral protein that is related to plasma protein inhibitors of serine proteases.

    abstract::Several recombinant cowpox viruses were constructed and used to identify a viral gene that controls the production of hemorrhage in lesions caused by the Brighton Red strain of cowpox virus (CPV-BR). This gene is located in the KpnD fragment of CPV-BR DNA, between 31 and 32 kilobases from the end of the genome. This p...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.83.20.7698

    authors: Pickup DJ,Ink BS,Hu W,Ray CA,Joklik WK

    更新日期:1986-10-01 00:00:00

  • Hilbert transforms in yukawan potential theory.

    abstract::If H denotes the classical Hilbert transform and Hu(x) = v(x), then the functions u(x) and v(x) are the values on the real axis of a pair of conjugate functions, harmonic in the upper half-plane. This note gives a generalization of the above concepts in which the Laplace equation Deltau = 0 is replaced by the Yukawa e...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.69.12.3677

    authors: Duffin RJ

    更新日期:1972-12-01 00:00:00

  • Continuous 1.3-million-year record of East African hydroclimate, and implications for patterns of evolution and biodiversity.

    abstract::The transport of moisture in the tropics is a critical process for the global energy budget and on geologic timescales, has markedly influenced continental landscapes, migratory pathways, and biological evolution. Here we present a continuous, first-of-its-kind 1.3-My record of continental hydroclimate and lake-level ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 历史文章,杂志文章

    doi:10.1073/pnas.1512864112

    authors: Lyons RP,Scholz CA,Cohen AS,King JW,Brown ET,Ivory SJ,Johnson TC,Deino AL,Reinthal PN,McGlue MM,Blome MW

    更新日期:2015-12-22 00:00:00

  • Conformation of lysine vasopressin: a comparison with oxytocin.

    abstract::Starting with assignments of proton nuclear magnetic resonance previously made for oxytocin in deuterated dimethylsulfoxide at 220 MHz, we have assigned resonances for the mammalian antidiuretic hormone, lysine vasopressin. The results demonstrate that spectral assignments of neurohypophyseal hormones and their congen...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.69.7.1920

    authors: Walter R,Glickson JD,Schwartz IL,Havran RT,Meienhofer J,Urry DW

    更新日期:1972-07-01 00:00:00