Abstract：
:Bcl-2 was isolated from the t(14;18) chromosomal breakpoint in follicular B-cell lymphoma. Bcl-2 has the unique oncogenic role of extending cell survival by inhibiting a variety of apoptotic deaths. An emerging family of Bcl-2-related proteins share two highly conserved regions referred to here as Bcl-2 homology 1 and 2 (BH1 and BH2) domains (Fig. 1). This includes Bax which heterodimerizes with Bcl-2 and when overexpressed counteracts Bcl-2. We report here that site-specific mutagenesis of Bcl-2 establishes the two domains as novel dimerization motifs. Substitution of Gly 145 in BH1 domain or Trp 188 in BH2 domain completely abrogated Bcl-2's death-repressor activity in interleukin-3 deprivation, gamma-irradiation and glucocorticoid-induced apoptosis. Mutations that affected Bcl-2's function also disrupted its heterodimerization with Bax, yet still permitted Bcl-2 homodimerization. These results establish a functional role for the BH1 and BH2 domains and suggest Bcl-2 exerts its action through heterodimerization with Bax.
journal_name
Naturejournal_title
Natureauthors
Yin XM,Oltvai ZN,Korsmeyer SJdoi
10.1038/369321a0subject
Has Abstractpub_date
1994-05-26 00:00:00pages
321-3issue
6478eissn
0028-0836issn
1476-4687journal_volume
369pub_type
杂志文章相关文献
NATURE文献大全abstract：:The first zygotic genes to be expressed during early Drosophila development are the gap genes. Their role is to read and interpret coarse positional information deposited in the egg by the mother and to refine it by cross-regulatory interactions and by controlling a class of pair-rule genes. Little is known about the ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/341335a0
更新日期：1989-09-28 00:00:00
abstract：:Two main forms of long-term potentiation (LTP)-a prominent model for the cellular mechanism of learning and memory-have been distinguished in the mammalian brain. One requires activation of postsynaptic NMDA (N-methyl d-aspartate) receptors, whereas the other, called mossy fibre LTP, has a principal presynaptic compon...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/415327a
更新日期：2002-01-17 00:00:00
abstract：:In the cores of some clusters of galaxies the hot intracluster plasma is dense enough that it should cool radiatively in the cluster's lifetime, leading to continuous 'cooling flows' of gas sinking towards the cluster centre, yet no such cooling flow has been observed. The low observed star-formation rates and cool ga...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature11379
更新日期：2012-08-16 00:00:00
abstract：:The maintenance of genome stability during mitosis is coordinated by the spindle assembly checkpoint (SAC) through its effector the mitotic checkpoint complex (MCC), an inhibitor of the anaphase-promoting complex (APC/C, also known as the cyclosome)1,2. Unattached kinetochores control MCC assembly by catalysing a chan...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-018-0281-1
更新日期：2018-07-01 00:00:00
abstract：: ...
journal_title：Nature
pub_type： 新闻
doi：10.1038/d41586-019-00012-4
更新日期：2019-01-01 00:00:00
abstract：:Enzymes are the subset of proteins that catalyse the chemistry of life, transforming both macromolecular substrates and small molecules. The precise three-dimensional architecture of enzymes permits almost unerring selectivity in physical and chemical steps to impose remarkable rate accelerations and specificity in pr...
journal_title：Nature
pub_type： 杂志文章,评审
doi：10.1038/35051697
更新日期：2001-01-11 00:00:00
abstract：:Glucagon is a 29-amino-acid peptide released from the α-cells of the islet of Langerhans, which has a key role in glucose homeostasis. Glucagon action is transduced by the class B G-protein-coupled glucagon receptor (GCGR), which is located on liver, kidney, intestinal smooth muscle, brain, adipose tissue, heart and p...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature17414
更新日期：2016-05-12 00:00:00
abstract：:To mediate its survival and virulence, the malaria parasite Plasmodium falciparum exports hundreds of proteins into the host erythrocyte. To enter the host cell, exported proteins must cross the parasitophorous vacuolar membrane (PVM) within which the parasite resides, but the mechanism remains unclear. A putative Pla...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature13574
更新日期：2014-07-31 00:00:00
abstract：:Large mammals, including humans, save much of the energy needed for running by means of elastic structures in their legs and feet. Kinetic and potential energy removed from the body in the first half of the stance phase is stored briefly as elastic strain energy and then returned in the second half by elastic recoil. ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/325147a0
更新日期：1987-01-08 00:00:00
abstract：:Transition-metal complexes are widely used in the physical and biological sciences. They have essential roles in catalysis, synthesis, materials science, photophysics and bioinorganic chemistry. Our understanding of transition-metal complexes originates from Alfred Werner's realization that their three-dimensional sha...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-019-1616-2
更新日期：2019-10-01 00:00:00
abstract：:With the current shortage in fetal bovine serum, researchers are turning to a variety of options for cell and tissue culture. ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/334547a0
更新日期：1988-08-11 00:00:00
abstract：:The capacity of the rough endoplasmic reticulum (RER) membrane of eukaryotic cells in translocate nascent presecretory proteins from the cytosol to the intracisternal space is preserved on cell fractionation and can be assayed in vitro. Two attempts to characterize this translocation activity have been reported. Warre...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/286174a0
更新日期：1980-07-10 00:00:00
abstract：:Interleukin-2 (IL-2) has a key role in the antigen-specific clonal growth of T lymphocytes, by virtue of its interaction with a specific cell-surface receptor (IL-2R). The growth signal seems to be delivered by IL-2 bound to the high-affinity, but not the low-affinity, receptor. Genes encoding IL-2 and its receptor (t...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/318467a0
更新日期：1985-12-05 00:00:00
abstract：:The battle for survival between bacteria and the viruses that infect them (phages) has led to the evolution of many bacterial defence systems and phage-encoded antagonists of these systems. Clustered regularly interspaced short palindromic repeats (CRISPR) and the CRISPR-associated (cas) genes comprise an adaptive imm...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature15254
更新日期：2015-10-01 00:00:00
abstract：:Since Bridgman's discovery of five solid water (H2O) ice phases1 in 1912, studies on the extraordinary polymorphism of H2O have documented more than seventeen crystalline and several amorphous ice structures2,3, as well as rich metastability and kinetic effects4,5. This unique behaviour is due in part to the geometric...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-019-1114-6
更新日期：2019-05-01 00:00:00
abstract：:Calcium signalling in astrocytes couples changes in neural activity to alterations in cerebral blood flow by eliciting vasoconstriction or vasodilation of arterioles. However, the mechanism for how these opposite astrocyte influences provide appropriate changes in vessel tone within an environment that has dynamic met...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature07525
更新日期：2008-12-11 00:00:00
abstract：:Do biological motors move with regular steps? To address this question, we constructed instrumentation with the spatial and temporal sensitivity to resolve movement on a molecular scale. We deposited silica beads carrying single molecules of the motor protein kinesin on microtubules using optical tweezers and analysed...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/365721a0
更新日期：1993-10-21 00:00:00
abstract：:Sensory transduction in taste and olfaction, the principal chemical senses, seems to be mediated by membrane-associated proteins on the apical surfaces of the respective receptor cells. The recent isolation and characterization of soluble 'odorant-binding proteins' secreted from the nasal glands of rat, cow and frog, ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/343366a0
更新日期：1990-01-25 00:00:00
abstract：:In several well-characterized site-specific recombination systems it has been shown that, for efficient recombination, the two recombining sites must have identical DNA sequences across the region between the staggered points of exchange. The precise DNA sequence of this overlap region, however, appears to be of littl...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/332861a0
更新日期：1988-04-28 00:00:00
abstract：:Over the past 50 years there has been much speculation about the energetic cost of swimming and wave-riding by dolphins. When aligned properly in front of the bow of moving ships in the stern wake of small boats, on wind waves, and even in the wake of larger cetaceans, the animals appear to move effortlessly through t...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/355821a0
更新日期：1992-02-27 00:00:00
abstract：:In obesity and type 2 diabetes, expression of the GLUT4 glucose transporter is decreased selectively in adipocytes. Adipose-specific Glut4 (also known as Slc2a4) knockout (adipose-Glut4(-/-)) mice show insulin resistance secondarily in muscle and liver. Here we show, using DNA arrays, that expression of retinol bindin...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature03711
更新日期：2005-07-21 00:00:00
abstract：:Knowledge of the contribution that individual countries have made to global radiative forcing is important to the implementation of the agreement on "common but differentiated responsibilities" reached by the United Nations Framework Convention on Climate Change. Over the past three decades, China has experienced rapi...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature17165
更新日期：2016-03-17 00:00:00
abstract：:The Ras proto-oncogene is a central component of mitogenic signal-transduction pathways, and is essential for cells both to leave a quiescent state (G0) and to pass through the G1/S transition of the cell cycle. The mechanism by which Ras signalling regulates cell-cycle progression is unclear, however. Here we report ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/386177a0
更新日期：1997-03-13 00:00:00
abstract：:Alzheimer's disease is a progressive loss of memory and cognition, for which there is no cure. Although genetic studies initially suggested a primary role for amyloid-in Alzheimer's disease, treatment strategies targeted at reducing amyloid-have failed to reverse cognitive symptoms. These clinical findings suggest tha...
journal_title：Nature
pub_type： 杂志文章,评审
doi：10.1038/nature20412
更新日期：2016-11-10 00:00:00
abstract：:Pheromone-based behaviours are crucial in animals from insects to mammals, and reproductive isolation is often based on pheromone differences. However, the genetic mechanisms by which pheromone signals change during the evolution of new species are largely unknown. In the sexual communication system of moths (Insecta:...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature09058
更新日期：2010-07-22 00:00:00
abstract：:Large, actively swimming suspension feeders evolved several times in Earth's history, arising independently from groups as diverse as sharks, rays and stem teleost fishes, and in mysticete whales. However, animals occupying this niche have not been identified from the early Palaeozoic era. Anomalocarids, a group of st...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature13010
更新日期：2014-03-27 00:00:00
abstract：:For more than four decades, transistors have been shrinking exponentially in size, and therefore the number of transistors in a single microelectronic chip has been increasing exponentially. Such an increase in packing density was made possible by continually shrinking the metal-oxide-semiconductor field-effect transi...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature10676
更新日期：2011-11-16 00:00:00
abstract：:For parallel computers to simulate our brains, we must face the fact that human beings have a better claim on the title Homo seriatim than Homo sapiens--we're more consistently serial than wise. ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/330033a0
更新日期：1987-11-05 00:00:00
abstract：:Rheumatoid arthritis is a chronic autoinflammatory disease that affects 1-2% of the world's population and is characterized by widespread joint inflammation. Interleukin-1 is an important mediator of cartilage destruction in rheumatic diseases, but our understanding of the upstream mechanisms leading to production of ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature13322
更新日期：2014-08-07 00:00:00
abstract：:Since the discovery of the first exoplanets, it has been known that other planetary systems can look quite unlike our own. Until fairly recently, we have been able to probe only the upper range of the planet size distribution, and, since last year, to detect planets that are the size of Earth or somewhat smaller. Hith...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature11914
更新日期：2013-02-28 00:00:00
