Abstract:
:Many small RNAs contribute essential activities to eukaryotic cells. In mammalian genomes dispersed repetitive sequences which exhibit homology to small RNAs often exist as pseudogenes which can complicate identification, localization, and analysis of the authentic gene. We mapped a productive human 7SK small nuclear RNA gene to human chromosome 6 by analyzing Northern blots derived from a panel of somatic cell hybrids that contain single human chromosomes. In order to avoid crossreactivity of the probe with rodent 7SK RNA, which is 98% identical to human 7SK, a method termed homolog exclusion probing was developed. This method uses an excess of non-labelled rodent-specific oligodeoxynucleotide to block the rodent 7SK RNA from hybridizing with the human-specific oligodeoxynucleotide probe. The effectiveness of this method to enhance the human 7SK RNA signal is demonstrated. The potential to map and subsequently isolate other small RNA genes by this approach and the use of homolog exclusion probing to discriminate among family members of highly related RNAs and DNAs in a single species is discussed.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Driscoll CT,Darlington GJ,Maraia RJdoi
10.1093/nar/22.5.722subject
Has Abstractpub_date
1994-03-11 00:00:00pages
722-5issue
5eissn
0305-1048issn
1362-4962journal_volume
22pub_type
杂志文章abstract::Alternative splicing of mRNA precursors results in multiple protein variants from a single gene and is critical for diverse cellular processes and development. Xist encodes a long noncoding RNA which is a central player to induce X-chromosome inactivation in female mammals and has two major splicing variants: long and...
journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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doi:10.1093/nar/4.5.1595
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:1989-06-26 00:00:00
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更新日期:1994-10-11 00:00:00
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更新日期:1993-11-11 00:00:00
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journal_title:Nucleic acids research
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更新日期:2020-11-18 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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abstract::Members of the Tc1/mariner superfamily of transposable elements isolated from vertebrates are transpositionally inactive due to the accumulation of mutations in their transposase genes. A novel open reading frame-trapping method was used to isolate uninterrupted transposase coding regions from the genome of the frog s...
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更新日期:2011-07-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2004-01-01 00:00:00
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更新日期:2021-01-11 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:1988-09-12 00:00:00
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更新日期:2005-03-01 00:00:00