Abstract:
:In this report, we describe the isolation and characterization of six murine squamous cell carcinoma cell lines (BPCC) derived from carcinomas produced by a complete carcinogenesis protocol with benzo[a]pyrene (B[a]P). All six cell lines were tumorigenic to varying degrees in nude mice, and several were spontaneously metastatic to the lungs. The in vivo invasive potential of each BPCC cell line was determined using de-epithelialized tracheal xenotransplants into which cells were inoculated. This assay revealed positive association of tumor grade with in vivo invasiveness, yet no clear relationship to the spontaneous metastatic potential of the cell lines, suggestive that invasive potential is only one determinant of the overall metastatic phenotype. At the molecular level, all six BPCC cell lines revealed the absence of mutations in the H-ras oncogene and no amplification or rearrangement in the cycl 1/cyclin D1 putative oncogene. Analysis of the p53 tumor suppressor gene revealed a direct correlation between positive nuclear immunohistochemical staining of the p53 protein in four BPCC cell lines and the presence of p53 mutations identified by direct sequence analysis. The localization of mutations to exons 7 and 8 of the p53 gene and the detection of G to T transversions in two of the four cell lines bearing p53 mutations are in agreement with previous analyses of a large series of primary B[a]P-induced murine skin tumors. In addition, frameshift mutations were identified in two cell lines. The correlation of the biological and molecular properties of these BPCC cell lines with the known characteristics of primary squamous cell carcinomas induced by B[a]P indicates that these cell lines could be useful tools in elucidating the mechanisms of tumorigenesis of this important chemical carcinogen.
journal_name
Carcinogenesisjournal_title
Carcinogenesisauthors
Ruggeri BA,Bauer B,Zhang SY,Klein-Szanto AJdoi
10.1093/carcin/15.8.1613subject
Has Abstractpub_date
1994-08-01 00:00:00pages
1613-9issue
8eissn
0143-3334issn
1460-2180journal_volume
15pub_type
杂志文章相关文献
CARCINOGENESIS文献大全abstract::Genetic variants in multiple cellular pathways have been associated with an altered risk of oesophageal cancer. In this study, eight genes previously associated with an altered risk of oesophageal squamous cell carcinoma (OSCC) in European or Asian populations were investigated in two South African populations. We gen...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgr211
更新日期:2011-12-01 00:00:00
abstract::We previously demonstrated that a single dose of gamma-radiation (350 rads) was able to induce thymic lymphomas in C57BL mice when followed by promoting treatment with oral cyclosporine (CsA), a non-genotoxic immunosuppressant. We have now tested the efficacy of various doses of gamma-radiation as an initiator of CsA ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/12.1.43
更新日期:1991-01-01 00:00:00
abstract::The repair of u.v.-induced damage in human and rodent cells was investigated at the level of DNA loops attached to the nuclear matrix. After 2 h post-u.v. incubation, DNase I digestion studies revealed a 3- to 4-fold enrichment of repair-labeled DNA at the nuclear matrix in four xeroderma pigmentosum cell strains belo...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/7.6.995
更新日期:1986-06-01 00:00:00
abstract::Checkpoint kinase (CHEK) 2, a tumor suppressor gene, plays an essential role in the DNA damage checkpoint response cascade. We first investigated two polymorphisms in the proximal promoter of the CHEK2 gene and evaluated their associations with the risk of lung cancer in a case-control study using 500 incident lung ca...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgq089
更新日期:2010-07-01 00:00:00
abstract::The mutagenicity of the lipid peroxidation product, malondialdehyde (MDA), was measured in the lacZ alpha forward mutation assay using a recombinant M13 phage, M13MB102. Single-stranded M13MB102 DNA was reacted with MDA at neutral pH and the modified DNA was transformed into strains of Escherichia coli induced for the...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/16.1.93
更新日期:1995-01-01 00:00:00
abstract::The effects of alternating ad libitum feeding and 30% restriction of the dietary intake on the development of diethylnitrosamine (DEN)-induced hepatic neoplasia were investigated. Dietary restriction retarded the growth of glucose-6-phosphatase-deficient (G6Pd) preneoplastic foci and subsequently that of hepatocellula...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/12.2.311
更新日期:1991-02-01 00:00:00
abstract::To indicate how readily nitrosamines (NAms) diffuse into the esophagus, we measured diffusion rate (flux) through rat esophagus of dialkyl-NAms using side-by-side diffusion apparatuses. Mucosal and serosal flux at 37 degrees C of two NAms, each at 50 microM, was followed for 90 min by gas chromatography-thermal energy...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/20.5.825
更新日期:1999-05-01 00:00:00
abstract::The nicotine-derived nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) induces lung adenocarcinoma through formation of DNA adducts. Our previous research on susceptibility to tobacco-induced carcinogenesis focused on benzo[a]pyrene diol epoxide (BPDE) as the in vitro mutagen for phenotype measurements ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgm029
更新日期:2007-07-01 00:00:00
abstract::Celastrol is an anti-inflammatory natural triterpenoid, isolated from the herb Tripterygium wilfordii or thunder god vine. Here, we define mechanisms mediating anti-inflammatory activity of celastrol and demonstrate efficacy of a dietary celastrol supplement for chemoprevention of inflammation-driven carcinogenesis in...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgx115
更新日期:2018-01-12 00:00:00
abstract::Mutagenicity and carcinogenicity of the ubiquitous environmental pollutant benzo[a]pyrene is mediated via its reactive diol epoxide metabolite, anti-BPDE, with the predominant formation of N(2)-deoxyguanine adducts in genomic DNA. Polyclonal and monoclonal antibodies specific for (+/-)-anti-BPDE DNA adducts were used ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/16.9.2029
更新日期:1995-09-01 00:00:00
abstract::Induction of glutathione S-transferase (GST) enzymes can increase detoxification of carcinogens and reduce carcinogen-induced mutagenesis and tumorigenesis. To determine if the anticarcinogen ellagic acid induces cellular enzymes which detoxify carcinogens, we examined the effect of ellagic acid on the expression of g...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/16.3.665
更新日期:1995-03-01 00:00:00
abstract::The repair kinetics and sensitivity to inhibitors of DNA strand breaks caused by superoxide anion, hydrogen peroxide, benzoyl peroxide and anthralin have been studied and compared with strand breaks caused by well-studied agents such as ionizing radiation and bleomycin. The latter two agents are generally believed to ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/7.9.1511
更新日期:1986-09-01 00:00:00
abstract::Basic in vitro systems can be used to model and assess complex diseases, such as cancer. Recent advances in this field include the incorporation of multiple cell types and extracellular matrix proteins into three-dimensional (3D) models to recapitulate the structure, organization and functionality of live tissue in si...
journal_title:Carcinogenesis
pub_type: 杂志文章,评审
doi:10.1093/carcin/bgu108
更新日期:2014-08-01 00:00:00
abstract::S-Adenosyl-L-methionine (SAM) is a strong chemopreventive agent of rat liver carcinogenesis. Examination was made to determine whether inhibition by SAM of the development of preneoplastic liver lesions persists to SAM withdrawal in diethylnitrosamine-initiated F344 rats promoted with thiobenzamide (TB). The rats were...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/17.7.1533
更新日期:1996-07-01 00:00:00
abstract::Apoptosis is a morphologically and biochemically distinct form of cell death which determines specific patterns of tissue size and shape and balances cell proliferation. In the present study, the sequence of cellular proliferative alterations in urinary bladder epithelium associated with uracil-induced reversible urin...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/18.8.1485
更新日期:1997-08-01 00:00:00
abstract::Caucasian renal transplant recipients from Queensland, Australia have the highest non-melanoma skin cancer (NMSC) risk worldwide. Although ultraviolet light (UVR) exposure is critical, genetic factors also appear important. We and others have shown that polymorphism in the glutathione S-transferases (GST) is associate...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgh291
更新日期:2005-01-01 00:00:00
abstract::Heat treatment of foods containing reducing sugars and amino acids during cooking or sterilization triggers a sequence of non-enzymatic reactions collectively known as the Maillard reaction. 5-Hydroxymethylfurfural (HMF), one of the major intermediate products in the Maillard reaction, has been found to possess cytoto...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/15.10.2375
更新日期:1994-10-01 00:00:00
abstract::Histone deacetylase (HDAC) inhibitors have the potential to derepress epigenetically silenced genes in cancer cells, leading to cell cycle arrest and apoptosis. In the present study, we screened several garlic-derived small organosulfur compounds for their ability to inhibit HDAC activity in vitro. Among the organosul...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgn165
更新日期:2008-09-01 00:00:00
abstract::Isothiocyanates are anticarcinogenic phytochemicals found in cruciferous vegetables that both induce and are substrates for the gluthatione S-transferases (GSTs). The GSTs are phase II metabolizing enzymes involved in metabolism of various bioactive compounds. Functional polymorphisms in GST genes have been identified...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgm141
更新日期:2007-09-01 00:00:00
abstract::The effects of D-limonene and citrus fruit oils, i.e. orange oil and lemon oil, on 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)-induced neoplasia of the lungs and forestomach of female A/J mice were investigated. D-Limonene and the citrus fruit oils given p.o. 1 h prior to NNK, also administered p.o., inhibite...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/12.1.115
更新日期:1991-01-01 00:00:00
abstract::We have previously demonstrated that the active dihydrofolate reductase (DHFR) gene is efficiently repaired in Chinese hamster ovary (CHO) cells which remove only a small fraction of u.v.-induced pyrimidine dimers from the overall genome. Preferential DNA repair of essential genes may explain why the u.v. resistance o...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/8.9.1333
更新日期:1987-09-01 00:00:00
abstract::MicroRNAs (miRNAs) are endogenous non-coding RNAs, which play an essential role in the regulation of gene expression during carcinogenesis. The role of miRNAs in breast cancer has been thoroughly investigated, and although many miRNAs are identified as cancer related, little is known about their involvement in benign ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgt333
更新日期:2014-01-01 00:00:00
abstract::As renal cell carcinomas are characterized by the disappearance of the transcription factor LFB1, which is known to be primarily involved in gene regulation in the liver, we have measured the presence of LFB1 in rat hepatocellular carcinomas induced by diethylnitrosamine. The level of LFB1 binding activity in adenoid-...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/16.1.143
更新日期:1995-01-01 00:00:00
abstract::The mutagenicity of N-nitroso-N-benzyl-methylamine (NBzMA), N-benzyl-N-nitrosourea (BzNU) and N-methyl-N-nitrosourea (MNU) in Salmonella typhimurium strains was investigated. BzNU selectively mutated TA100 strain as compared to TA1535, whereas MNU showed an inverse strain response, an effect probably related to the fa...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/11.9.1653
更新日期:1990-09-01 00:00:00
abstract::In vitro exposures of isolated DNA to one of the two carcinogenic and mutagenic chemicals, diethylsulfate or dimethylsulfate, induces several kinds of physicochemical and morphological alterations. These changes are detectable by a variety of independent techniques. A fraction of DNA treated briefly with either of the...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/2.10.981
更新日期:1981-01-01 00:00:00
abstract::DNA-methyltransferase-3B (DNMT3B) plays an important role in the generation of aberrant methylation in carcinogenesis. Polymorphisms and haplotypes of the DNMT3B gene may influence DNMT3B activity on DNA methylation, thereby modulating the susceptibility to lung cancer. To test this hypothesis, we investigated the ass...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgh307
更新日期:2005-02-01 00:00:00
abstract::The H2-receptor antagonist, ranitidine, is among the most widely used pharmaceuticals to treat gastroesophageal reflux disease and peptic ulcers. While previous studies have demonstrated that amines can form N-nitrosamines when exposed to nitrite at stomach-relevant pH, N-nitrosamine formation from ranitidine, an amin...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgw034
更新日期:2016-06-01 00:00:00
abstract::To evaluate the significance of C-C chemokine receptor type 5 (CCR5) in lung tumor development, we compared carcinogen-induced tumor growth in CCR5 knockout (CCR5(-/-)) mice and wild-type (CCR5(+/+)) mice. CCR5(-/-) mice showed reduced urethane (1g/kg)-induced tumor incidence when compared with those of CCR5(+/+) mice...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgs265
更新日期:2012-12-01 00:00:00
abstract::The epidermal growth factor receptor (EGFR) pathway has recently been appreciated as a central mediator of tumorigenesis and an important drug target; however, the influence of genetic variation in this pathway on bladder cancer is not understood. Pathway activation leads to cell proliferation, angiogenesis and is ant...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgp077
更新日期:2009-07-01 00:00:00
abstract::Both Snell dwarf mice (dw/dw) and their phenotypically normal heterozygotes (dw/+) were dosed with methylclofenapate (MCP) at daily intervals by gavage (25 mg/kg). Animals were killed at 12, 24, 36 and 72 h after the initial dose and the parameters of ploidy, nuclearity and DNA synthesis were measured in hepatocytes i...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/11.3.387
更新日期:1990-03-01 00:00:00