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Formation of macromolecular complexes and other effects of DNA treatment with diethyl and dimethyl sulfate: physico-chemical and electron microscopic studies.

Abstract：

:In vitro exposures of isolated DNA to one of the two carcinogenic and mutagenic chemicals, diethylsulfate or dimethylsulfate, induces several kinds of physicochemical and morphological alterations. These changes are detectable by a variety of independent techniques. A fraction of DNA treated briefly with either of these two chemicals moves during velocity centrifugation experiments less rapidly than the bulk of control DNA and more rapidly through gels during electrophoresis. This apparent decrease in size is paralleled by the formation of large DNA aggregates with mobilities indicating molecular weights several times that of the untreated, control DNA. The presence of a basic protein in the incubation mixture increases the rate of formation of such complexes. the tendency of the alkylated DNA to bind to both biological and non-biological materials is reflected in the increased attachment of DNA to columns built with methyl-esterified serum albumin and in its quantitative retention on nitrocellulose filters. DNA exposed to dimethylsulfate decreases its density in CsCl gradients. A mixture of two or more DNAs of different densities exposed to this chemical produces an u.v.-absorbing band which is found in such gradients at an intermediate density. If the alkylation reaction is carried out in the presence of a protein, a portion of DNA bands at a density intermediate between the density of DNA and that of the protein, even in the presence of an ionic detergent in the gradient. Under the electron microscope the alkylated DNA shows multiple single-strand breaks and peeling-off whiskers of denatured DNA. Aggregates of DNA molecules become visible upon further incubation of DNA with the alkylating agent. We suggest that the DNA-DNA and DNA- protein complexes play an important role in the process of carcinogenesis and mutagenesis.

journal_name

Carcinogenesis

journal_title

Carcinogenesis

authors

Kubinski H,Kubinski ZO,Fiandt M,Konopa G

doi

10.1093/carcin/2.10.981

subject

Has Abstract

pub_date

1981-01-01 00:00:00

pages

981-90

issue

eissn

0143-3334

issn

1460-2180

journal_volume

pub_type

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Formation of macromolecular complexes and other effects of DNA treatment with diethyl and dimethyl sulfate: physico-chemical and electron microscopic studies.