Abstract：
:Effective solid-phase extraction, derivatization, and GC/MS procedures are developed for the simultaneous determinations of butalbital, amobarbital, pentobarbital, and secobarbital, using a deuterated pentobarbital (d5-pentobarbital) as the internal standard. Buffered (pH 7) urine samples were extracted with Bond Elute Certify II cartridge. Iodomethane/tetramethylammonium hydroxide in dimethylsulfoxide was used for methylation, while a HP 5970 MSD equipped with a 13 m J & W DB-5 column (5% phenyl polysiloxane phase) and the Thru-Put Target software package were used for GC/MS analysis and data processing. This protocol was found to be superior, in both chromatographic performance characteristics and quantitation results, over a liquid-liquid extraction procedure without derivatization using hexobarbital as the internal standard. Extraction recoveries observed from control samples containing four barbiturates range from 80% to 90%. Good one-point calibration data are obtained for all four barbiturates in the 50 to 3200 ng/mL range. Interestingly, the one-point calibration data for pentobarbital are inferior to the other three barbiturates--due to interference from the internal standard (d5-pentobarbital). The calibration data of pentobarbital are best described by a hyperbolic curve regression model. Precision data (% CV) for GC/MS analysis, over-all procedure, and day-to-day performance are approximately 2.0%, 6.0%, and 8.0%, respectively. With the use of a 2 mL sample size, the attainable detection limit is approximately 20 ng/mL.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
Liu RH,McKeehan AM,Edwards C,Foster G,Bensley WD,Langner JG,Walia ASsubject
Has Abstractpub_date
1994-11-01 00:00:00pages
1504-14issue
6eissn
0022-1198issn
1556-4029journal_volume
39pub_type
杂志文章abstract：:In the summer of 1984, police in Pinellas County, Florida, confiscated six identically colored imported Asian skulls (in a shipping case) from a private citizen. In May 1988, in nearby Hillsborough County, police confiscated a very similar skull from another private citizen, who allegedly had found it in an abandoned ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1990-05-01 00:00:00
abstract：:This report details a fingerprinting technique for softening mummified fingers and hands using Metaflow and Restorative, a fluid for rehydration of desiccated tissue. This procedure has proven successful in cases of extreme desiccation and has allowed for good fingerprint detail to be developed. ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1988-09-01 00:00:00
abstract：:The interpretation of postmortem cocaine concentrations is made in an attempt to estimate drug concentrations present at the time of death and thus infer not only drug presence but drug toxicity. Previous data suggest that changes in postmortem blood cocaine concentrations over time are not predictable and interpretat...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:A study was conducted of potential vapor phase interferents which could be present on human breath and also be capable of inducing a false-positive response for ethanol on the evidential infrared-based breath testing device, the Intoxilyzer-5000. This involved preparation and validation of a range of vapor standards, ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1997-11-01 00:00:00
abstract：:Four tribal populations of Andhra Pradesh, South India (1), Chenchu (n=100), Lambadi (n=107), Naikpod Gond (n=104) and Yerukula (n=101) were analyzed for DNA polymorphisms at 15 tetranucleotide and 2 pentanucleotide short tandem repeat (STR) loci in the present study. ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2005-07-01 00:00:00
abstract：:The U.S. agricultural sector is vulnerable to intentionally introduced microbial threats because of its wide and open distribution and economic importance. To investigate such events, forensically valid assays for plant pathogen detection are needed. In this work, real-time PCR assays were developed for three model pl...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12321
更新日期：2014-03-01 00:00:00
abstract：:Forensic science aims to serve society by advancing justice. It is accepted that some actions taken by the state in the interests of advancing justice, such as postmortem examinations, may impinge on values held by members of groups within society. Such actions have the potential to cause cultural offense. It is impor...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2008.00661.x
更新日期：2008-03-01 00:00:00
abstract：:The documentation of dental materials used in the USA during the WWII era is readily available, while references for the Japanese are minimal. It was therefore important to build a photographic database of Japanese restorative care which could be utilized as a comparison tool for the deployed odontologist. The dental ...
journal_title：Journal of forensic sciences
pub_type： 历史文章,杂志文章
doi：10.1111/1556-4029.13446
更新日期：2017-11-01 00:00:00
abstract：:The stability of five commonly prescribed barbiturates and thiopental in blood and liver at room temperature and at 4 degrees C was studied. Gas chromatography was used for oxybarbiturate analysis while liquid chromatography was used to quantitate thiopental. In blood and liver, greater than 75% of the drugs were dete...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：:This paper has reported a case of sudden unexpected death in a 19-year-old man, shot in the abdomen, who was surgically treated and discharged. He died of massive left retropleural hematoma from a spontaneous rupture of a traumatic pseudo-aneurysm of the abdominal aorta. Diagnosis of the retropleural hemorrhage and th...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1979-07-01 00:00:00
abstract：:Deoxyribonucleic acid (DNA) typing of ribosomal ribonucleic acid (rRNA) genes was performed with a polymerase chain reaction (PCR) assay for species identification. A variable region of the 28S ribosomal RNA gene was amplified with primers complementary to flanking sequences phylogenetically well conserved. The produc...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1992-03-01 00:00:00
abstract：:The United States Supreme Court, in the recent case of Riggins v. Nevada, extended its examination of the issue of involuntary treatment with anti-psychotic medication to the mentally disabled facing criminal trial. Although this was an extreme case where the defendant faced a possible death sentence, the involuntary ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1993-11-01 00:00:00
abstract：:Intercostal and age differences in the sternal rib end morphology of documented female skeletons from Spitalfields and St. Bride's are examined. The morphology was captured using three-dimensional morphometrics and the statistical analyses employed included parametric and nonparametric MANOVA, discriminant analysis, a...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12047
更新日期：2013-03-01 00:00:00
abstract：:This paper tests the Demirjian and international Demirjian dental aging methods for forensic use when ancestry and ethnicity are unknown. A radiographic sample of 187 boys and girls was collected from the Department of Pediatric Dentistry at the University of Detroit Mercy and aged using both methods. The total sample...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2011.01833.x
更新日期：2011-09-01 00:00:00
abstract：:Twenty-five permanent teeth, including eight carious ones whose pulp cavities had been exposed, were used for this research 3-5 weeks after extraction. Phosphate-buffered saline (PBS, at pH 7.2) was employed to extract ABO blood group substance from tooth powder. ABO grouping was performed on blood-stained compresses ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1993-07-01 00:00:00
abstract：:DNA was isolated from casework urine samples previously submitted for toxicological analysis. The quality and quantity of DNA isolated was determined by spectrofluorometry and agarose yield gel electrophoresis. Hae III restricted samples were then resolved by analytical agarose gel electrophoresis, transferred to a me...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1994-11-01 00:00:00
abstract：:Isolated death (ID) (i.e., dying alone without anyone noticing for several days) has been suggested to be related to social isolation, mental disorder, and alcohol and/or drug abuse. A major transfer of patients with a mental disorder and/or alcohol and/or drug abuse from institutionalized care to treatment as outpati...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2004-03-01 00:00:00
abstract：:This paper's objective is to describe two cases of fatal commotio cordis resulting from the deliberate striking of children's chests by adults with their fists. These deaths involve two male children, ages 3 years and 14 months. The clinical histories, events in the households prior to the deaths, behaviors of the chi...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2000-05-01 00:00:00
abstract：:Closed-circuit television (CCTV) footage is used in criminal investigations to compare perpetrators with suspects. Usually, incomplete gait cycles are collected, making evidential gait analysis challenging. This study aimed to analyze the discriminatory power of joint angles throughout a gait cycle. Six sets from 12 m...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12322
更新日期：2014-03-01 00:00:00
abstract：:The in vitro accuracy and precision of four infrared breath-alcohol analyzers, the Alcotest 7010, BAC Verifier, Intoxilyzer 5000, and Intoximeter 3000, were studied with a protocol adapted from portions of the U.S. Department of Transportation's (DOT) "Standard for Devices to Measure Breath Alcohol." Statistical evalu...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：:Medico-legal death investigation in China has a long history that can be traced back to the Chhin era in the third century BC. We conducted a national study on the current medico-legal death investigation system in China. Our study showed that, at present, medico-legal death investigations in China are conducted by c....
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2011.01792.x
更新日期：2011-07-01 00:00:00
abstract：:A basilar skull fracture that extends along both petrous bones is generally considered to be a response to impacts to the lateral aspects of the head. This generalization is not warranted as such a fracture often results from impacts to any point around the base of the skull or to the chin. These facts were appreciate...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1980-04-01 00:00:00
abstract：:The sequences of the two hypervariable (HV) segments of the mitochondrial DNA (mtDNA) control region were determined in 167 randomly selected, unrelated individuals living in the state of Alagoas, north-eastern Brazil. One hundred and forty-five different haplotypes, associated with 139 variable positions, were determ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2007.00619.x
更新日期：2008-01-01 00:00:00
abstract：:Two hundred and twenty-eight consecutive medicolegal autopsies were studied as to the cause of death established by a physician, the mode of death as suggested in the police report, and findings of medical interest often discovered following autopsy. Corrections had to be made even in some cases which seemed to be obv...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1988-03-01 00:00:00
abstract：:Hunting gazelle is an offense according to Israeli law. When comparative isoelectric focusing was performed on bloodstains made from gazelle, goat, sheep, and cow blood, the pattern obtained from gazelle hemoglobin differed from those of the other animals tested. The use of this difference in hemoglobin pattern is sug...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1989-03-01 00:00:00
abstract：:A population of persons who were found, at death, to have significant levels of isopropyl alcohol (isopropanol) in their blood is described. The role of isopropanol in causing death is reviewed in 57 fatal cases. The contribution of acetone, the major metabolite of isopropanol, to death is assessed. Results indicate t...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1982-07-01 00:00:00
abstract：:A case is presented involving a young woman on several illicit drugs (heroin, cocaine and cannabis) as well as two medications and a solvent used for their anesthetic and narcotic properties: thiopental, ketamine and chloroform. This complex drug use was supported by hair analysis over a 10.5 cm segment of the hair ta...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1998-03-01 00:00:00
abstract：:This study evaluates the accuracy and precision of a skeletal age estimation method, using the acetabulum of 100 male ossa coxae from the Grant Collection (GRO) at the University of Toronto, Canada. Age at death was obtained using Bayesian inference and a computational application (IDADE2) that requires a reference po...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2011.01700.x
更新日期：2011-03-01 00:00:00
abstract：:We tested a new approach to the stature prediction that could be used in the identification process of human skeletal remains of unknown identity. The stature of 19 female and 21 male adult cadavers was measured within 24 h after death and considered to be equal to the living stature. The antero-posterior radiographs ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2007.00419.x
更新日期：2007-05-01 00:00:00
abstract：:Dissolving bodies is a current method of disposing of human remains and has been practiced throughout the years. During the last decade in the Netherlands, two cases have emerged in which human remains were treated with acid. In the first case, the remains of a cremated body were treated with hydrofluoric acid. In the...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12700
更新日期：2015-05-01 00:00:00
