Abstract:
:The U.S. agricultural sector is vulnerable to intentionally introduced microbial threats because of its wide and open distribution and economic importance. To investigate such events, forensically valid assays for plant pathogen detection are needed. In this work, real-time PCR assays were developed for three model plant pathogens: Pseudomonas syringae pathovar tomato, Xylella fastidiosa, and Wheat streak mosaic virus. Validation included determination of the linearity and range, limit of detection, sensitivity, specificity, and exclusivity of each assay. Additionally, positive control plasmids, distinguishable from native signature by restriction enzyme digestion, were developed to support forensic application of the assays. Each assay displayed linear amplification of target nucleic acid, detected 100 fg or less of target nucleic acid, and was specific to its target pathogen. Results obtained with these model pathogens provide the framework for development and validation of similar assays for other plant pathogens of high consequence.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
James M,Blagden T,Moncrief I,Burans JP,Schneider K,Fletcher Jdoi
10.1111/1556-4029.12321subject
Has Abstractpub_date
2014-03-01 00:00:00pages
463-9issue
2eissn
0022-1198issn
1556-4029journal_volume
59pub_type
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