Development of Multiple Assays using 46 SNPs for Comprehensive mtDNA Haplogrouping and Application to Highly Degraded DNA.

Abstract:

:Six multiplex PCR systems using single-base extension reactions to analyze 46 mitochondrial DNA (mtDNA)-coding region single nucleotide polymorphisms (SNPs) that define 42 haplogroups, that is, 24 major mtDNA haplogroups and 18 subclades, were devised. To improve the usefulness of the established systems for the analysis of degraded DNA samples, novel primers to render amplicons with sizes <150 bp were designed. By applying these systems to 214 Japanese individuals, 24 different haplogroups (power of discrimination = 93.4%) were found. To assess the effectiveness of our systems in grouping degraded DNA, an ancient bone sample of a Jomon skeleton was analyzed and then classified as haplogroup N9b. We conclude that the present systems are powerful screening tools for major haplogroups of mtDNA in addition to the prevalent subhaplogroups in the Japanese population and that these systems are capable of analyzing highly degraded DNA samples in forensic studies.

journal_name

J Forensic Sci

authors

Nunotani M,Sato N,Kamei S,Shiozaki T,Hayashi T,Asamura H

doi

10.1111/1556-4029.12961

subject

Has Abstract

pub_date

2016-03-01 00:00:00

pages

472-477

issue

2

eissn

0022-1198

issn

1556-4029

journal_volume

61

pub_type

杂志文章
  • Forensic Analysis of Cigarette Ash-Brand Determination Through Trace-metal Analysis.

    abstract::The information inherent in cigarette ash in the form of trace-metal concentrations may be of use in a forensic context as it can indicate the brand from which the ash originated. This knowledge might help place suspects at crime scenes or determine how many people may have been present. To develop and test statistica...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/1556-4029.13040

    authors: Groth AC,Barnes JH,Lewis C,Murray CK,Albahadily F,Jourdan TH

    更新日期:2016-07-01 00:00:00

  • Effects of the Taser in fatalities involving police confrontation.

    abstract::Sixteen deaths associated with the use of the Taser were examined. All involved young males who had a history of abuse of controlled substances; all but three were under the influence of cocaine, phencyclidine [phenylcyclohexylpiperidine (PCP)], or amphetamine. All were behaving in a bizarre or unusual fashion which n...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Kornblum RN,Reddy SK

    更新日期:1991-03-01 00:00:00

  • A fast preparation of skeletal materials using enzyme maceration.

    abstract::The current study investigates the removal of soft tissues from mice and rats by the use of three different proteases and one lipase from Novozymes A/S. The results demonstrate the enzyme maceration to be remarkably fast (1-3 h) compared to the traditional warm-water procedure, which requires up to several days. In ad...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/j.1556-4029.2010.01668.x

    authors: Simonsen KP,Rasmussen AR,Mathisen P,Petersen H,Borup F

    更新日期:2011-03-01 00:00:00

  • Stalking following the breakup of romantic relationships: characteristics of stalking former partners.

    abstract::This study investigated female experiences of stalking by former partners following the dissolution of heterosexual romantic relationships. It aimed to identify those characteristics of former partners that were associated with stalking as opposed to other post-relationship experiences of minor harassment or no-harass...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Roberts KA

    更新日期:2002-09-01 00:00:00

  • Postmortem concentrations of citalopram.

    abstract::The postmortem concentrations of citalopram in blood, bile, liver, and vitreous humour were investigated in 14 cases using a specially developed high performance liquid chromatography assay. Concentrations from drug and non-drug related deaths were categorized to determine a postmortem therapeutic and toxic range. The...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Anastos N,McIntyre IM,Lynch MJ,Drummer OH

    更新日期:2002-07-01 00:00:00

  • Death caused by a signal rocket--not an isolated case*.

    abstract::Fatalities related to sporting events are predominantly caused by blunt force injuries especially due to the emotional involvement of crowd, but occasionally other types of trauma are reported as well. A case of very rare trauma caused by shooting with a hand-held parachute signal rocket during a football match is pre...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/j.1556-4029.2008.00791.x

    authors: Alempijević D,Jecmenica D,Pavlekić S,Savić S,Kovacević S

    更新日期:2008-09-01 00:00:00

  • Systematic differences in electropherogram peak heights reported by different versions of the GeneScan software.

    abstract::DNA profiling using STRs on the 310 and 3100 Genetic Analyzers routinely generates electropherograms that are analyzed with the GeneScan software available from the instrument's manufacturer, Applied Biosystems. Users have been able to choose from three different smoothing options that have been known to result in sig...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Gilder JR,Ford S,Doom TE,Raymer ML,Krane DE

    更新日期:2004-01-01 00:00:00

  • A comparison of sexual victimization in the childhoods of pedophiles and hebephiles.

    abstract::The association between perpetration of sexual abuse and the offender's own victimization as a child has been well documented in the literature. Various researchers have examined this relationship by assessing the exclusiveness of the sexual abuser's behavior, the gender of his victims and the gender of his own childh...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Greenberg DM,Bradford JM,Curry S

    更新日期:1993-03-01 00:00:00

  • Visualization and quantification of air embolism structure by processing postmortem MSCT data.

    abstract::Venous air embolism (VAE) is an often occurring forensic finding in cases of injury to the head and neck. Whenever found, it has to be appraised in its relation to the cause of death. While visualization and quantification is difficult at traditional autopsy, Magnetic Resonance Imaging (MRI) and Computed Tomography (C...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Jackowski C,Thali M,Sonnenschein M,Aghayev E,Yen K,Dirnhofer R,Vock P

    更新日期:2004-11-01 00:00:00

  • Toolmark identification of a mattock to a clod of soil from a grave.

    abstract::At a recent bushland gravesite that contained a deceased male, a clod of soil displaying a striated toolmark was collected during the excavation of the grave. This clod was preserved, and the mark was cast using Mikrosil™. Shovels and a mattock, which had been discarded by the suspects, were subsequently found at anot...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/j.1556-4029.2010.01537.x

    authors: Clark MD

    更新日期:2011-01-01 00:00:00

  • Histologic evidence of repetitive blunt force abdominal trauma in four pediatric fatalities.

    abstract::In cases of acute fatal child abuse, certain injuries, including cutaneous blunt force trauma, skull fractures, subdural hematomas, intra-abdominal hemorrhage, and retinal hemorrhages are common and well described in the pediatric and forensic literature. These gross findings at autopsy, when taken into consideration ...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/j.1556-4029.2008.00883.x

    authors: Dye DW,Peretti FJ,Kokes CP

    更新日期:2008-11-01 00:00:00

  • Characterization of the products formed by the reaction of trichlorocyanuric acid with 2-propanol.

    abstract::We report a recent investigation into the death of a cat that was initially thought to involve intentionally burning the animal via the use of an ignitable liquid. The exposure of the animal to flame was ruled out. Instead, forensic investigation revealed the intentional mixing together of a common outdoor swimming po...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/j.1556-4029.2009.01181.x

    authors: Sandercock PM,Barnett JS

    更新日期:2009-11-01 00:00:00

  • Driver and front seat passenger fatalities associated with air bag deployment. Part 1: A Canadian study.

    abstract::Real world motor vehicle collision research of injuries due to deployment of "first-generation" air bags has been conducted by Transport Canada since 1993. Fifty-three fatal crashes (36 frontal impacts; 17 side collisions) involving 48 drivers and 10 right front passengers were reviewed. In the Canadian data, air bag ...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Shkrum MJ,McClafferty KJ,Nowak ES,German A

    更新日期:2002-09-01 00:00:00

  • AmpliType PM and HLA DQ alpha typing from pap smear, semen smear, and postcoital slides.

    abstract::Deoxyribonucleic acid (DNA) samples extracted from stained pap smear, semen smear and postcoital slides were amplified by the polymerase chain reaction (PCR) and typed for the AmpliType PM and HLA DQ alpha alleles. HLA DQ alpha and PM types consistent with blood controls from the donors were obtained. Stained cells fi...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Roy R,Reynolds R

    更新日期:1995-03-01 00:00:00

  • Nonmetric sex determination from the distal and posterior humerus in black and white South Africans.

    abstract::The successful identification of human skeletal remains relies on proven diagnostic techniques for sex determination. This research utilized 608 individuals from South Africa (420 men, 188 women) to conduct a blind nonmetric determination of sex from three features of the distal humerus: olecranon fossa shape, angle o...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/j.1556-4029.2011.01724.x

    authors: Vance VL,Steyn M,L'Abbé EN

    更新日期:2011-05-01 00:00:00

  • Massively Parallel Sequencing of Forensic STRs Using the Ion Chef™ and the Ion S5™ XL Systems.

    abstract::Next-generation sequencing (NGS) has been used to genotype forensic short tandem repeat (STR) markers for individual identification and kinship analysis. STR data from several NGS platforms have been published, but forensic application trials using the Ion S5™ XL system have not been reported. In this work, we report ...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/1556-4029.13767

    authors: Wang L,Chen M,Wu B,Liu YC,Zhang GF,Jiang L,Xu XL,Zhao XC,Ji AQ,Ye J

    更新日期:2018-11-01 00:00:00

  • Terrorism--what can the psychiatrist do?

    abstract::The 1970s have yielded many situations in which psychiatrists have been summoned by agents of the state for help in resolving terrorist-related crises. This paper tries to examine the evolving role of forensic psychiatrists as they participate in such situations and reviews some of the functions involved in open commu...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Daly LN

    更新日期:1981-01-01 00:00:00

  • 3D forensic facial approximation: Implementation protocol in a forensic activity.

    abstract::The primary objective of this paper is to report on the successful implementation of forensic facial approximation in a real case in the forensic context. A three-dimensional (3D) facial approximation protocol of the skull was performed with free software, applying techniques in a virtual environment that have already...

    journal_title:Journal of forensic sciences

    pub_type:

    doi:10.1111/1556-4029.14587

    authors: Baldasso RP,Moraes C,Gallardo E,Stumvoll MB,Crespo KC,Strapasson RAP,de Oliveira RN

    更新日期:2021-01-01 00:00:00

  • Bayesian adaptive approach to estimating sample sizes for seizures of illicit drugs.

    abstract::A considerable amount of discussion can be found in the forensics literature about the issue of using statistical sampling to obtain for chemical analyses an appropriate subset of units from a police seizure suspected to contain illicit material. Use of the Bayesian paradigm has been suggested as the most suitable sta...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/j.1556-4029.2011.01930.x

    authors: Moroni R,Aalberg L,Reinikainen T,Corander J

    更新日期:2012-01-01 00:00:00

  • Toxicological findings in fatal motor vehicle collisions in ontario, Canada: a one-year study.

    abstract::Drug-impaired driving is a complex area of forensic toxicology due in part to limited data concerning the type of drugs involved and the concentrations detected. This study analyzed toxicological findings in drivers from fatal motor vehicle collisions (FMVCs) in Ontario, Canada, over a one-year period using a standard...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/1556-4029.12725

    authors: Woodall KL,Chow BL,Lauwers A,Cass D

    更新日期:2015-05-01 00:00:00

  • Statistical discrimination of footwear: a method for the comparison of accidentals on shoe outsoles inspired by facial recognition techniques.

    abstract::In the field of forensic footwear examination, it is a widely held belief that patterns of accidental marks found on footwear and footwear impressions possess a high degree of "uniqueness." This belief, however, has not been thoroughly studied in a numerical way using controlled experiments. As a result, this form of ...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/j.1556-4029.2009.01209.x

    authors: Petraco ND,Gambino C,Kubic TA,Olivio D,Petraco N

    更新日期:2010-01-01 00:00:00

  • A fatality involving phentermine.

    abstract::A case is presented where phentermine, a sympathomimetic amine used as an anorectic drug, is believed to have significantly contributed to the death of an individual in whom other drugs were also found. Blood, urine, and tissue concentrations of phentermine are reported and compared to other cases in the literature. ...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Levine B,Caplan YH,Dixon AM

    更新日期:1984-10-01 00:00:00

  • The postmortem fate of Pat Gregory: a disinterred Native American.

    abstract::In late June 1990, the Mono County Sheriffs Department in Bridgeport, CA contacted the Physical Anthropology Human Identification Laboratory (PAHIL) at California State University, Chico to seek assistance in the identification of a recently discovered skull. To assist with possible identification, the cranium receive...

    journal_title:Journal of forensic sciences

    pub_type: 历史文章,杂志文章

    doi:

    authors: Murad TA,Murad TD

    更新日期:2000-03-01 00:00:00

  • Improved gas chromatography/mass spectrometry analysis of barbiturates in urine using centrifuge-based solid-phase extraction, methylation, with d5-pentobarbital as internal standard.

    abstract::Effective solid-phase extraction, derivatization, and GC/MS procedures are developed for the simultaneous determinations of butalbital, amobarbital, pentobarbital, and secobarbital, using a deuterated pentobarbital (d5-pentobarbital) as the internal standard. Buffered (pH 7) urine samples were extracted with Bond Elut...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:

    authors: Liu RH,McKeehan AM,Edwards C,Foster G,Bensley WD,Langner JG,Walia AS

    更新日期:1994-11-01 00:00:00

  • Suicide trends in Upper Egypt.

    abstract::Suicide is an important problem, ranking among the top 10 causes of death for individuals in all ages in developed countries. This article is a retrospective study evaluating suicide cases in Assiut, one of the largest provinces in Egypt, from 2005 to 2009. There were 117 cases, of which involved 68 male victims (58.1...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章,收录出版

    doi:10.1111/j.1556-4029.2012.02247.x

    authors: Abdel Moneim WM,Yassa HA,George SM

    更新日期:2012-09-01 00:00:00

  • Stress and deception in speech: evaluating layered voice analysis.

    abstract::This study was designed to evaluate commonly used voice stress analyzers--in this case the layered voice analysis (LVA) system. The research protocol involved the use of a speech database containing materials recorded while highly controlled deception and stress levels were systematically varied. Subjects were 24 each...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/j.1556-4029.2009.01026.x

    authors: Harnsberger JD,Hollien H,Martin CA,Hollien KA

    更新日期:2009-05-01 00:00:00

  • Individuals lacking ridge detail: A case study in adermatoglyphia.

    abstract::Adermatoglyphia is a very rare autosomal-dominant condition that is genetically inherited and causes an individual to be born without conventional ridge detail on either their palmar or plantar surfaces (the fingers and palms of the hands and the toes and the soles of the feet). While adermatoglyphia has been the focu...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/1556-4029.14597

    authors: Cook HI,Harrison K,James H

    更新日期:2021-01-01 00:00:00

  • Current practices by forensic anthropologists in adult skeletal age estimation.

    abstract::When determining an age estimate from adult skeletal remains, forensic anthropologists face a series of methodological choices. These decisions, such as which skeletal region to evaluate, which methods to apply, what statistical information to use, and how to combine information from multiple methods, ultimately impac...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/j.1556-4029.2011.01979.x

    authors: Garvin HM,Passalacqua NV

    更新日期:2012-03-01 00:00:00

  • Yield of illicit indoor cannabis cultivation in the Netherlands.

    abstract::To obtain a reliable estimation on the yield of illicit indoor cannabis cultivation in The Netherlands, cannabis plants confiscated by the police were used to determine the yield of dried female flower buds. The developmental stage of flower buds of the seized plants was described on a scale from 1 to 10 where the val...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/j.1556-4029.2006.00228.x

    authors: Toonen M,Ribot S,Thissen J

    更新日期:2006-09-01 00:00:00

  • Correlation between saw blade width and kerf width.

    abstract::Most studies of saw marks have focused on morphological characteristics and their utility in identifying saws suspected to have been utilized in cases of criminal dismemberment. The present study examined the extent to which metric analysis may be used to correlate saw blade measurements with minimum kerf widths (MKWs...

    journal_title:Journal of forensic sciences

    pub_type: 杂志文章

    doi:10.1111/1556-4029.14556

    authors: Menschel M,Pokines JT,Reinecke G

    更新日期:2021-01-01 00:00:00