Systematic differences in electropherogram peak heights reported by different versions of the GeneScan software.

Abstract:

:DNA profiling using STRs on the 310 and 3100 Genetic Analyzers routinely generates electropherograms that are analyzed with the GeneScan software available from the instrument's manufacturer, Applied Biosystems. Users have been able to choose from three different smoothing options that have been known to result in significant differences in the peak heights that are reported. Improvements in the underlying algorithm of the most recent version of the software also result in significant and somewhat predictable differences in peak height values. Laboratories that have performed validation studies using older versions of GeneScan should either reanalyze the data generated in those validation studies with the newest version of the software or otherwise take into consideration the systematically higher peak height values obtained as they begin following the recommendation of the manufacturer and use the new algorithm.

journal_name

J Forensic Sci

authors

Gilder JR,Ford S,Doom TE,Raymer ML,Krane DE

keywords:

subject

Has Abstract

pub_date

2004-01-01 00:00:00

pages

92-5

issue

1

eissn

0022-1198

issn

1556-4029

journal_volume

49

pub_type

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