Abstract：
:A screening assay has been developed to provide preliminary individualization of crime scene samples thus eliminating expensive, time-consuming short tandem repeat (STR) profiling of nonprobative samples. High resolution melting performed in a real-time PCR instrument is used to detect the slight melting differences between the length and sequence variations of 22 forensic STRs. Three STRs (vWA, D18S51, THO1) were chosen to develop an assay which was optimized for Mg++ concentration, annealing/extension time/temperature, assay volume, and bovine serum albumin addition. The assay was tested for reproducibility, uniformity for genotype, melting profile consistency, effects of inhibitors, and mixture effects. The assay could be used to determine DNA concentration when a standard curve is run simultaneously. Calculations of costs show that the assay can save significant time and money for a crime with many samples or suspects.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
Nicklas JA,Noreault-Conti T,Buel Edoi
10.1111/j.1556-4029.2011.01981.xsubject
Has Abstractpub_date
2012-03-01 00:00:00pages
478-88issue
2eissn
0022-1198issn
1556-4029journal_volume
57pub_type
杂志文章abstract：:Identification of sex from the skeleton is an important demographic assessment in medicolegal investigations. Studies have demonstrated that populations differ from each other in size and proportions and that these differences can affect the metric assessment of sex. It is therefore vital to determine if population di...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
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更新日期：1998-09-01 00:00:00
abstract：:Recent court challenges have highlighted the need for statistical research on fingerprint identification. This paper proposes a model for computing likelihood ratios (LRs) to assess the evidential value of comparisons with any number of minutiae. The model considers minutiae type, direction and relative spatial relati...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2006.00327.x
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abstract：:Over the past decade, there has been a growing openness about the importance of human factors in forensic work. However, most of it focused on cognitive bias, and neglected issues of workplace wellness and stress. Forensic scientists work in a dynamic environment that includes common workplace pressures such as worklo...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13533
更新日期：2018-01-01 00:00:00
abstract：:Air embolism may rarely cause sudden death during or shortly after pregnancy. Certain obstetric techniques, douching procedures, and blowing air into the vagina have been associated with this phenomenon. A case of venous air embolism in a pregnant woman occurring during sexual intercourse in a rear entry position is p...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1983-10-01 00:00:00
abstract：:This paper describes three recent false sexual assaults examined at the Victoria Forensic Science Centre laboratory where clothing damage analysis assisted in the resolution of the case. Suspected false reports of sexual assaults are often sensitive cases with little other forensic evidence. Any evidential value that ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2000-05-01 00:00:00
abstract：:For the past 10 years, medical imaging techniques have been increasingly applied to forensic investigations. To obtain histological and toxicological information, tissue and liquid samples are required. In this article, we describe the development of a low-cost, secure, and reliable approach for a telematic add-on for...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2011.01990.x
更新日期：2012-03-01 00:00:00
abstract：:Since psychiatrists may be held legally accountable for their patients' violent acts against others, psychiatrists on inpatient wards are faced with the dilemma of deciding whether or not, and when, to discharge improved but still possibly dangerous patients. This paper presents a survey of an inner-city hospital's ex...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1987-07-01 00:00:00
abstract：:The presence of interfering substances, particularly acetone, has historically been a concern in the forensic measurement of ethanol in human breath. Although modern infrared instruments employ methods for distinguishing between ethanol and acetone, false-positive interferant results can arise from instrumental or pro...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1994-07-01 00:00:00
abstract：:We report the identification of acetone (0.45 mg/mL) and isopropanol (0.17 mg/mL) but without the presence of ethanol in a blood sample from a man suspected of driving under the influence of alcohol. A preliminary breath screening test with an electrochemical instrument (Alcolmeter S-L2) was positive and an evidential...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1995-07-01 00:00:00
abstract：:During postmortem examination of the dental arches of carbonized victims, dental restorative materials may be found. The aim of this study was to evaluate the effect of heat source action on the color stability of composite resin (CR) and glass ionomer cement (GIC) restorations, to discriminate between them and compar...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13292
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1981-01-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1996-11-01 00:00:00
abstract：:Next-generation sequencing (NGS) has been used to genotype forensic short tandem repeat (STR) markers for individual identification and kinship analysis. STR data from several NGS platforms have been published, but forensic application trials using the Ion S5™ XL system have not been reported. In this work, we report ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13767
更新日期：2018-11-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1990-07-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12888
更新日期：2016-01-01 00:00:00
abstract：:Microbial biomarkers that indicate aspects of an organism's growth conditions are important targets of forensic research. In this study, we examined fatty acid composition as a signature for the types of complex nutrients in the culturing medium. Bacillus cereus T-strain spores were grown in medium formulations supple...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12771
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2002-05-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2010.01356.x
更新日期：2010-05-01 00:00:00
abstract：:The Organization of Scientific Area Committees defines threshold of identification as the minimum concentration of ignitable liquid identifiable from gas chromatographic-mass spectrometry data using accepted pattern identification criteria. We propose a method for determining this threshold for gasoline based on base ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13983
更新日期：2019-07-01 00:00:00
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journal_title：Journal of forensic sciences
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doi：
更新日期：2002-09-01 00:00:00
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journal_title：Journal of forensic sciences
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doi：
更新日期：2002-01-01 00:00:00
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journal_title：Journal of forensic sciences
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doi：
更新日期：1993-07-01 00:00:00
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2002-11-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：:Cardiac rhabdomyomas are rare lesions forming part of the tuberous sclerosis complex that may be responsible for sudden death. As well as remaining clinically occult for variable periods of time, they may, along with other manifestations of tuberous sclerosis, be quite difficult to detect clinically and pathologically...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1991-07-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1996-05-01 00:00:00
abstract：:The beginning of latent fingerprint development on porous surface was first achieved by silver nitrate (AgNO3 ) method. But the significantly increasing cost has caused forensic experts to look for an alternative means. Silver nitrate (AgNO3 ) is the main component in the synthesis of metal nanoparticles, namely silve...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.14664
更新日期：2021-01-08 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1998-05-01 00:00:00
