Abstract：
:DNA was isolated from casework urine samples previously submitted for toxicological analysis. The quality and quantity of DNA isolated was determined by spectrofluorometry and agarose yield gel electrophoresis. Hae III restricted samples were then resolved by analytical agarose gel electrophoresis, transferred to a membrane by Southern blotting and hybridized with a chemiluminescently-labelled (D2S44) probe. The DNA fragment banding patterns were indistinguishable from the DNA banding patterns of blood specimens collected from the same donor. Only 5 of 20 samples yielded banding patterns and the banding intensity relative to background was low. Genomic DNA was also obtained from casework samples by Chelex extraction, amplified by polymerase chain reaction (PCR) and then genotyped for human leucocyte antigen (HLA) DQ alpha. Of 20 specimens, 13 (65%) were typed correctly producing identical results for urine and blood specimens obtained from the same donor. Aging studies of casework samples and normal samples (from a non-drug using population) were also conducted with PCR-HLA DQ alpha analysis. Results of these studies indicate that amplification by PCR was more likely to produce positive results. Based on these findings, we conclude that PCR-initiated analysis is more suitable than RFLP analysis for individualization of urine samples.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
Medintz I,Chiriboga L,McCurdy L,Kobilinsky Lsubject
Has Abstractpub_date
1994-11-01 00:00:00pages
1372-80issue
6eissn
0022-1198issn
1556-4029journal_volume
39pub_type
杂志文章abstract：:We report on the use of a surgically implanted device, an EBI osteostimulator, as a means of establishing the identification of a homicide victim. The use of such an appliance for securing positive identification has not been previously reported. Additionally we stress the importance of intensive and accurate excavati...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1999-11-01 00:00:00
abstract：:In developing latent prints on cartridge casings and shotgun shells, multiple chemical processes should be used in order to obtain the best results. In Phase I, this study established an optimal chemical sequence for both Brass and Nickel cartridge casings based on six sequences involving four chemicals: Cyanoacrylate...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2009.01152.x
更新日期：2009-11-01 00:00:00
abstract：:An ELISA method for the detection of salivary amylase in dried stains using a monoclonal anti-human salivary amylase antibody was developed. Studies demonstrated the assay to be sensitive down to 0.0002 Sigma units and showed a linear response between absorbance and salivary amylase activity between 0.002 and 0.2 unit...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2005-07-01 00:00:00
abstract：:The rise in improved and widely accessible printing technology has resulted in an interest to develop rapid and minimally destructive chemical analytical techniques that can characterize printing inks for forensic document analysis. Chemical characterization of printing inks allows for both discrimination of inks orig...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13107
更新日期：2016-05-01 00:00:00
abstract：:We investigated toxicological and pharmacogenetic factors that could influence methadone toxicity using postmortem samples. R- and S-methadone were measured in femoral blood from 90 postmortem cases, mainly drug users. The R-enantiomer concentrations significantly exceeded that of the S-enantiomers (Wilcoxon's test, p...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2009.01278.x
更新日期：2010-03-01 00:00:00
abstract：:It is generally accepted that nuclei degrade in developing hair shafts but the point at which such occurs has not been investigated. The fate of mitochondria in the keratinizing hair shaft has been less clear. This study uses transmission electron microscopy to investigate when nuclei and mitochondria are no longer vi...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2008.00972.x
更新日期：2009-03-01 00:00:00
abstract：:A case has been reported which adds a new and unusual dimension to false rape allegation and gives a heretofore unknown motive for a woman to claim a bogus rape. A female newspaper reporter entered a hospital, stated falsely that she had been sexually assaulted, allowed herself to be interviewed and then examined by t...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1975-10-01 00:00:00
abstract：:Soil DNA profiling has potential as a forensic tool to establish a link between soil collected at a crime scene and soil recovered from a suspect. However, a quantitative measure is needed to investigate the spatial/temporal variability across multiple scales prior to their application in forensic science. In this stu...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13741
更新日期：2018-07-01 00:00:00
abstract：:In order to establish an animal model for entomotoxicological studies, the kinetics of morphine elimination from blood after a single intravenous injection of morphine and the concentration of morphine in tissues following a continuous perfusion were studied. The aim of these experiments was to obtain controlled morph...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1999-03-01 00:00:00
abstract：:Internal standards are commonly used for the quantitative determination of drugs of abuse and their metabolites (drug/metabolite) in biological fluids and tissues by the selective ion monitoring (SIM) gas chromatography/mass spectrometry (GC/MS) procedure. Analogs of drugs/metabolites that are labeled with three or mo...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
abstract：:We evaluated the homogeneity of drug concentrations in muscle in 14 cadavers, comprising 11 drug overdoses and three cases of chronic therapeutic drug use. Analyses were performed on samples from twelve named muscles and femoral venous blood. Standard analytical techniques and instrumentation were used throughout. The...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:Continuous monitoring of existing methods of skeletal diagnosis allows improving the reliability of personal identification in forensic and archaeological contexts. This study reports on a blind test re-evaluating the sexing technique proposed by Rogers (8) involving the distal humerus. A total of 351 humeri (184 male...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2005-11-01 00:00:00
abstract：:Hunting gazelle is an offense according to Israeli law. When comparative isoelectric focusing was performed on bloodstains made from gazelle, goat, sheep, and cow blood, the pattern obtained from gazelle hemoglobin differed from those of the other animals tested. The use of this difference in hemoglobin pattern is sug...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1989-03-01 00:00:00
abstract：:The main disadvantage to gunshot residue (GSR) particle analysis utilizing scanning electron microscope/energy dispersive X-ray (SEM/EDX) instrumentation has been the excessive operator time required for search and identification. This study uses an automated particle search and characterization program for unattended...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:An analysis was performed on a prehistoric human vertebral column recovered during an archaeological excavation in the Sierra of northern California. Fifteen burials believed to represent the Maidu culture of 400 to 700 years ago were recovered. The vertebral column from one of the individuals is particularly signific...
journal_title：Journal of forensic sciences
pub_type： 历史文章,杂志文章
doi：
更新日期：1985-07-01 00:00:00
abstract：:The movement toward private sector involvement in our correctional services and programs is growing. Before our focus is turned completely to privatization of these services, it would be prudent to analyze the "policy impact of such change. It is evident that the diverse and incompatible policies guiding the governmen...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:Analysis of length polymorphism at short tandem repeat (STR) loci utilizing the polymerase chain reaction (PCR) process has proven to be an ideal assay for human identification purposes. The short length of STR loci coupled with the amplification of target sequence through PCR allows for a robust, sensitive, and speci...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2004-11-01 00:00:00
abstract：:From the works of Broca and Krogman to modern-day Jantz and Buikstra, the orbit has been used for both quantitative and qualitative sex and race estimation. This study evaluates the practical value of these estimations. Orbital height and breadth were measured to determine the orbital index and assess differences betw...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2011.01864.x
更新日期：2011-11-01 00:00:00
abstract：:Cellular immune response is accompanied by the release of neopterin. Increased neopterin levels in urine and serum are observed in patients during viral infections, autoimmune diseases, and allograft rejections and certain malignant diseases. We investigated postmortem neopterin concentrations in urine and serum sampl...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1991-07-01 00:00:00
abstract：:Age estimation methods are often based on the age-related morphological changes of the auricular surface and the pubic bone. In this study, a mathematical approach to quantify these changes has been tested analyzing the curvature variation on 3D models from CT and laser scans. The sample consisted of the 24 Suchey-Bro...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12689
更新日期：2015-05-01 00:00:00
abstract：:Pre- and post-transfusion blood samples were collected from 22 subjects together with the corresponding plucked hair samples taken 2 days and 2 weeks after the transfusion. The phosphoglucomutase1 (PGM1) subphenotypes of blood and hair were determined by isoelectric focusing and the phenotypes confirmed by gel electro...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1984-04-01 00:00:00
abstract：:The likelihood of dipteran maggots colonizing a corpse due to nocturnal oviposition can be used to challenge the postmortem interval (PMI) estimated assuming diurnal oviposition. Earlier experiments tested nocturnal oviposition behavior by exposing fresh baits once during a single night. In this pilot study, ovipositi...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2009.01095.x
更新日期：2009-09-01 00:00:00
abstract：:Differentiating between black powder toners used in laser printers and copiers can be challenging for forensic examiners. One hundred and sixty-two samples from 82 different types of cartridges produced by 21 manufacturers that are currently available on the Polish market were studied using Fourier transform infrared ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2006.00167.x
更新日期：2006-07-01 00:00:00
abstract：:The heme compound found in deoxyribonucleic acid (DNA) extracted from bloodstains, which is regarded as a major inhibitor of polymerase chain reaction (PCR), was characterized in comparison with alkaline and acid hematin, histidine and ammonia hemochromogens, and globin and serum albumin hemochromogens digested by pro...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1994-03-01 00:00:00
abstract：:Bismuth oxychloride (BiOCl) was the first viable synthetic pearl pigment developed 50 years ago. It was only used for a limited time period in automotive paint (model years 1998-2000), serving to produce luster for a single Chrysler black metallic color. Identification of this pigment in an unknown automotive paint ca...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12414
更新日期：2014-09-01 00:00:00
abstract：:The recent decline in the incidence of the SIDS in the District of Columbia has permitted identification of what appears to be two separate groups of cases within the generic SIDS population. While deaths of bed-sharing victims remained relatively constant throughout the study period, a greater than fourfold decrease ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1978-04-01 00:00:00
abstract：:Identification of sex from skeleton parts is of paramount importance to medicolegal investigations. The present study makes an attempt to assess sex from the ulna by devising new measurements for fragmentary bones. Dry and adult ulnae (100 male and 60 female) of Madhya Pradesh, India were subjected to three measuremen...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2001-07-01 00:00:00
abstract：:We studied sexually dimorphic differences in the ilium using geometric morphometric analysis of 10 osteometric landmarks recorded by multislice computed tomography, based on three-dimensional reconstructions of 188 children (95 boys, 93 girls) of mixed origins living in the area of Toulouse, southern France, and rangi...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12037
更新日期：2013-03-01 00:00:00
abstract：:Photographic surveying is fundamental to crime scene investigation. It is performed using 2D images; however, this method is limited because it does not offer a detailed view. We present a 3D (three-dimensional) experimental model that has clarified unsolved aspects of a homicide. A woman was found dead in her home. C...
journal_title：Journal of forensic sciences
pub_type：
doi：10.1111/1556-4029.14041
更新日期：2019-09-01 00:00:00
abstract：:The in vitro accuracy and precision of four infrared breath-alcohol analyzers, the Alcotest 7010, BAC Verifier, Intoxilyzer 5000, and Intoximeter 3000, were studied with a protocol adapted from portions of the U.S. Department of Transportation's (DOT) "Standard for Devices to Measure Breath Alcohol." Statistical evalu...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
