Abstract：
:Analysis of length polymorphism at short tandem repeat (STR) loci utilizing the polymerase chain reaction (PCR) process has proven to be an ideal assay for human identification purposes. The short length of STR loci coupled with the amplification of target sequence through PCR allows for a robust, sensitive, and specific assay for highly polymorphic markers. A multiplex containing fifteen STR loci plus the gender-determining locus Amelogenin was developed to provide a single amplification/detection of all CODIS (Combined DNA Index System) STR loci (CSF1PO, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, FGA, TH01, TPOX, and vWA) as well as two internationally-accepted STRs (D2S1338 and D19S433). By incorporating five-dye fragment analysis technology and non-nucleotide linkers, previously optimized AmpFlSTR kit primer sequences have been maintained. This kit has been developed in accordance with the standards of the forensic community as defined by the DNA Advisory Board. Validation studies were performed to include developmental validation, and the results support the use of the AmpFlSTR Identifiler PCR Amplification Kit for human identity and parentage testing.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
Collins PJ,Hennessy LK,Leibelt CS,Roby RK,Reeder DJ,Foxall PAkeywords:
subject
Has Abstractpub_date
2004-11-01 00:00:00pages
1265-77issue
6eissn
0022-1198issn
1556-4029journal_volume
49pub_type
杂志文章abstract：:The association between perpetration of sexual abuse and the offender's own victimization as a child has been well documented in the literature. Various researchers have examined this relationship by assessing the exclusiveness of the sexual abuser's behavior, the gender of his victims and the gender of his own childh...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1993-03-01 00:00:00
abstract：:The fortuitous recovery of material trapped within the cavity of a hollow-point bullet at autopsy was demonstrated to be of evidentiary value. Analysis of this material, as well as that from hollow-point bullets from test-firings, showed that the contents, especially if in multiple layers, could be correlated with the...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1988-07-01 00:00:00
abstract：:Experiments were performed to determine the extent of cross-contamination of DNA resulting from secondary transfer due to fingerprint brushes used on multiple items of evidence. Analysis of both standard and low copy number (LCN) STR was performed. Two different procedures were used to enhance sensitivity, post-PCR cl...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12911
更新日期：2016-01-01 00:00:00
abstract：:The physiological, psychological, and social consequences associated with illicit drug use are well documented. In addition to the effects directly related to the drug(s), the delivery mechanism can precipitate other serious health conditions. A case is reported where an individual stopped by law enforcement was disco...
journal_title：Journal of forensic sciences
pub_type：
doi：10.1111/1556-4029.13573
更新日期：2018-03-01 00:00:00
abstract：:A new set of multiplexed PCR primers has been applied to the analysis of human skeletal remains to determine their efficacy in analyzing degraded DNA. These primer sets, known as Miniplexes, produce shorter amplicons (50-280 base pairs (bp)) than standard short tandem repeat (STR) kits, but still utilize the 13 CODIS ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2006.00077.x
更新日期：2006-03-01 00:00:00
abstract：:Differentiation of 21 glitter lip-glosses from seven manufacturers was attempted by pyrolysis gas chromatography/mass spectroscopy. Samples were pyrolyzed on a ribbon probe at 800 degrees C for 20 sec and analyzed with an Agilent 6890N Network GC System and Agilent 5973 Network Mass Selective Detector with MSD Product...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2009.01109.x
更新日期：2009-09-01 00:00:00
abstract：:A forensic genotyping panel of 11 tetranucleotide STR loci from the domestic cat was characterized and evaluated for genetic individualization of cat tissues. We first examined 49 candidate STR loci and their frequency assessment in domestic cat populations. The STR loci (3-4 base pair repeat motifs), mapped in the ca...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2005-09-01 00:00:00
abstract：:Two hundred and twenty-eight consecutive medicolegal autopsies were studied as to the cause of death established by a physician, the mode of death as suggested in the police report, and findings of medical interest often discovered following autopsy. Corrections had to be made even in some cases which seemed to be obv...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1988-03-01 00:00:00
abstract：:The pubic symphysis is among the most commonly used bilateral age indicators. Because of potential differences between right and left sides, it is necessary to investigate within-individual asymmetry, which can inflate age estimation error. This study uses 3D laser scans of paired pubic symphyses for 88 documented Whi...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13871
更新日期：2019-03-01 00:00:00
abstract：:A principal goal of this research was to conduct a field evaluation of "on-site" multi-analyte drug testing devices to determine the most accurate, efficient, and cost-effective device available for the purpose of rapidly detecting drivers under the influence of drugs. Four on-site kits were selected and evaluated for...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1998-03-01 00:00:00
abstract：:In the summer of 1984, police in Pinellas County, Florida, confiscated six identically colored imported Asian skulls (in a shipping case) from a private citizen. In May 1988, in nearby Hillsborough County, police confiscated a very similar skull from another private citizen, who allegedly had found it in an abandoned ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1990-05-01 00:00:00
abstract：:High-energy synchrotron radiation x-ray fluorescence spectrometry (SR-XRF) utilizing 116 keV x-rays was used to characterize titanium dioxide pigments (rutile) and automotive white paint fragments for forensic examination. The technique allowed analysis of K lines of 9 trace elements in 18 titanium dioxide pigments (r...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2009.01005.x
更新日期：2009-05-01 00:00:00
abstract：POPULATION:Blood samples were collected from a total of 84 healthy and unrelated Halakki (44) and Kunabhi (40) populations, with their informed written consent. The geographic location of the sampled area is shown in Fig. 1. Both the populations are endogamous, and they belong to Dravidian linguistic family. Halakki is...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2005.00038.x
更新日期：2006-01-01 00:00:00
abstract：:In cases of acute fatal child abuse, certain injuries, including cutaneous blunt force trauma, skull fractures, subdural hematomas, intra-abdominal hemorrhage, and retinal hemorrhages are common and well described in the pediatric and forensic literature. These gross findings at autopsy, when taken into consideration ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2008.00883.x
更新日期：2008-11-01 00:00:00
abstract：:This study introduces a new method of determining sex based on four morphological features of the posterior, distal humerus. The technique was developed on a 20th century anatomy series, the University of Toronto Grant Skeletal Collection, and was tested on 35 known individuals from the University of New Mexico Docume...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:The successful identification of human skeletal remains relies on proven diagnostic techniques for sex determination. This research utilized 608 individuals from South Africa (420 men, 188 women) to conduct a blind nonmetric determination of sex from three features of the distal humerus: olecranon fossa shape, angle o...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2011.01724.x
更新日期：2011-05-01 00:00:00
abstract：:The aims of this study were to enable geometric morphometric sex classification using tibial proximal and distal sexual dimorphism and to evaluate the secular trend of tibial shape/form from the early 20th century to the present day. The study samples consisted of 61 adult tibias from an early 20th-century Czech popul...
journal_title：Journal of forensic sciences
pub_type： 历史文章,杂志文章
doi：10.1111/1556-4029.12641
更新日期：2015-01-01 00:00:00
abstract：:There are no published reports that include both timely antemortem and postmortem carbamazepine concentrations after massive overdose. We report a fatal overdose of carbamazepine with both timely antemortem and postmortem carbamazepine concentrations. Carbamazepine concentrations were 47.7 mcg/mL 2 h antemortem and 53...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2001-11-01 00:00:00
abstract：:While the mitochondrial control region has proven successful for human forensic evaluations by indicating ethnic origin, domestic dogs (Canis lupus familiaris) of seemingly unrelated breeds often form large groups based on identical control region sequences. In an attempt to break up these large haplotype groups, we h...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2008.00952.x
更新日期：2009-03-01 00:00:00
abstract：:This study examined the effects of fire on the features associated with saw marks in bone. Both class and individual characteristics were examined using stereomicroscopy and scanning electron microscopy (SEM). Twenty-four semifleshed Sus scrofa L. tibiae were sawed into three sections with the middle section having de...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12580
更新日期：2015-01-01 00:00:00
abstract：:A case of homicide by the intravenous injection of Energine, a petroleum distillate spot remover, is presented. This case is the only known homicide committed with naphtha. This elderly man had severe natural disease in addition to chest trauma sustained in the assault leading to death; however, the rapid injection of...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
abstract：:A study was conducted of potential vapor phase interferents which could be present on human breath and also be capable of inducing a false-positive response for ethanol on the evidential infrared-based breath testing device, the Intoxilyzer-5000. This involved preparation and validation of a range of vapor standards, ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1997-11-01 00:00:00
abstract：:Timing of oviposition on a corpse is a key factor in entomologically based minimum postmortem interval (mPMI) calculations. However, there is considerable variation in nocturnal oviposition behavior of blow flies reported in the research literature. This study investigated nocturnal oviposition in central England for ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12841
更新日期：2015-11-01 00:00:00
abstract：:Opium is the raw material for the production of heroin, and the characterization of opium seizures through laboratory analysis is a valuable tool for law enforcement agencies to trace clandestine opium production and trafficking. In this work, a method for opium profiling based on the relative content of five principa...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13190
更新日期：2016-11-01 00:00:00
abstract：:The American alligator inhabits bodies of fresh water in Florida and other southeastern states. Although attacks on pets are frequent, alligator attacks on humans are relatively rare because of the animal's natural fear of man. Because of the rarity of attacks on humans, the pathologic findings and pathophysiology of ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2006.00135.x
更新日期：2006-05-01 00:00:00
abstract：:Visualization of nitrite residues is essential in gunshot distance determination. Current protocols for the detection of nitrites include, among other tests, the Modified Griess Test (MGT). This method is limited as nitrite residues are unstable in the environment and limited to partially burned gunpowder. Previous re...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13802
更新日期：2019-01-01 00:00:00
abstract：:Forensic DNA interpretation is transitioning from manual interpretation based usually on binary decision-making toward computer-based systems that model the probability of the profile given different explanations for it, termed probabilistic genotyping (PG). Decision-making by laboratories to implement probability-bas...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13898
更新日期：2019-03-01 00:00:00
abstract：:Deoxyribonucleic acid (DNA) samples extracted from stained pap smear, semen smear and postcoital slides were amplified by the polymerase chain reaction (PCR) and typed for the AmpliType PM and HLA DQ alpha alleles. HLA DQ alpha and PM types consistent with blood controls from the donors were obtained. Stained cells fi...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1995-03-01 00:00:00
abstract：:Two case studies are presented involving fatal falls of adult females from a height. One involved a launch at low speed from a balcony, and one involved a launch at high speed from the top of a cliff. Crime scene evidence obtained on the balcony itself provided a strong indication of homicide, but subsequent investiga...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2005.00026.x
更新日期：2006-01-01 00:00:00
abstract：:Measurements of the type and concentration of propellant and stabilizer additives in smokeless gunpowder are used by forensic scientists investigating the source of explosives and by military laboratories assuring the safety and efficacy of munitions. The National Institute of Standards and Technology recently assesse...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2002-11-01 00:00:00
