Mitochondrial genome DNA analysis of the domestic dog: identifying informative SNPs outside of the control region.

Abstract:

:While the mitochondrial control region has proven successful for human forensic evaluations by indicating ethnic origin, domestic dogs (Canis lupus familiaris) of seemingly unrelated breeds often form large groups based on identical control region sequences. In an attempt to break up these large haplotype groups, we have analyzed the remaining c. 15,484 base pairs of the canine mitochondrial genome for 79 dogs and used phylogenetic and population genetic methods to search for additional variability in the form of single nucleotide polymorphisms (SNPs). We have identified 356 SNPs and 65 haplotypes in the remainder of the mitochondrial genome excluding the control region. The exclusion capacity was found to be 0.018. The mitochondrial control region was also evaluated for the same 79 dogs. The signals from the different fragments do not conflict, but instead support one another and provide a larger fragment of DNA that can be analyzed as forensic evidence.

journal_name

J Forensic Sci

authors

Webb KM,Allard MW

doi

10.1111/j.1556-4029.2008.00952.x

subject

Has Abstract

pub_date

2009-03-01 00:00:00

pages

275-88

issue

2

eissn

0022-1198

issn

1556-4029

pii

JFO952

journal_volume

54

pub_type

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