Abstract：
:Respiratory pathogens have been detected in forensic investigations using multiple techniques; however, no study has examined the use of automated, nested, multiplex polymerase chain reaction (ANM-PCR), commonly used in living patients, in the forensic setting. This retrospective study assessed the utility of ANM-PCR in detecting respiratory pathogens in the pediatric forensic setting. Respiratory samples from 35 cases were tested for up to 20 respiratory pathogens. 51.4% of these cases yielded a positive ANM-PCR result, 20% of which were considered the cause of or contributory to death. The most commonly detected pathogens were rhinovirus/enterovirus and respiratory syncytial virus, and these were the only pathogens determined to play a significant role in cause of death. The sampled sites and postmortem intervals tested did not affect the likelihood of a positive or negative test. ANM-PCR panels are effective, affordable, and rapid ancillary tools in evaluating cause of death in the forensic pediatric population.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
Baker T,Schandl C,Presnell SE,Madory J,Nolte FS,Batalis Ndoi
10.1111/1556-4029.13415subject
Has Abstractpub_date
2017-09-01 00:00:00pages
1223-1228issue
5eissn
0022-1198issn
1556-4029journal_volume
62pub_type
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