Abstract:
:The quality and efficiency of a standard organic DNA isolation method and a silica-based method using the QIAGEN Blood Maxi Kit were compared to obtain human DNA and short tandem repeats (STRs) profiles from 39 exhumed bone samples for paternity testing. DNA samples were quantified by real-time PCR, and STR profiles were obtained using the AmpFlSTR(®) Identifiler(®) PCR amplification kit. Overall, the silica-based method recovered less DNA ranging from 0 to 147.7 ng/g (average 7.57 ng/g, median = 1.3 ng/g) than did the organic method ranging from 0 to 605 ng/g (average 44.27 ng/g, median = 5.8 ng/g). Complete profiles (16/16 loci tested) were obtained from 37/39 samples (95%) using the organic method and from 9/39 samples (23%) with the silica-based method. Compared with a standard organic DNA isolation method, our results indicate that the published silica-based method does not improve neither the quality nor the quantity of DNA for STR profiling.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
Rucinski C,Malaver AL,Yunis EJ,Yunis JJdoi
10.1111/j.1556-4029.2011.02012.xsubject
Has Abstractpub_date
2012-05-01 00:00:00pages
706-12issue
3eissn
0022-1198issn
1556-4029journal_volume
57pub_type
杂志文章abstract::When determining an age estimate from adult skeletal remains, forensic anthropologists face a series of methodological choices. These decisions, such as which skeletal region to evaluate, which methods to apply, what statistical information to use, and how to combine information from multiple methods, ultimately impac...
journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:10.1111/j.1556-4029.2011.01979.x
更新日期:2012-03-01 00:00:00
abstract::Although the ability to develop latent fingerprints on paper using heat alone has been noted previously, it has been considered impractical for casework and inferior to other techniques. Here a new refinement of the technique is demonstrated for the high quality development of latent fingerprints on porous surfaces su...
journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:10.1111/j.1556-4029.2008.00902.x
更新日期:2009-01-01 00:00:00
abstract::Borate-containing compounds were formerly used as topical antiseptics and were components of many medicinal preparations including skin powders and ointments used for the treatment of burns and diaper rash. These compounds were also used as irrigants for body cavities, including the pleural, vaginal, and rectal caviti...
journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:10.1111/j.1556-4029.2007.00420.x
更新日期:2007-05-01 00:00:00
abstract::Twenty-five permanent teeth, including eight carious ones whose pulp cavities had been exposed, were used for this research 3-5 weeks after extraction. Phosphate-buffered saline (PBS, at pH 7.2) was employed to extract ABO blood group substance from tooth powder. ABO grouping was performed on blood-stained compresses ...
journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:
更新日期:1993-07-01 00:00:00
abstract::Although there is considerable literature on grief and grief work, there have been few studies of the grief process in those who have killed someone. This paper reviews the scope of this problem in England and Wales and examines a number of issues in connection with the grieving process in those who have killed but, b...
journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:
更新日期:1990-09-01 00:00:00
abstract::In Italy, the "silent-consent" principle of donor's willingness regulates organ donation for postmortem transplantation, but civil incompetence excludes it. We investigated decisional capacity for organ donation for transplantation of 30 controls and 30 nonincompetent patients with schizophrenia as related to clinical...
journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:10.1111/j.1556-4029.2010.01364.x
更新日期:2010-05-01 00:00:00
abstract::The 1970s have yielded many situations in which psychiatrists have been summoned by agents of the state for help in resolving terrorist-related crises. This paper tries to examine the evolving role of forensic psychiatrists as they participate in such situations and reviews some of the functions involved in open commu...
journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:
更新日期:1981-01-01 00:00:00
abstract::A 1H nuclear magnetic resonance (1H NMR) method for the determination of gamma-hydroxybutyric acid (GHB) and gamma-hydroxybutyrolactone (GBL) in human serum and urine using spiked samples has been developed. The method gives linear responses (correlation coefficients of 0.99 or greater) over the concentration range 0....
journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract::A 48-year-old woman was found dead on a chair in her living room. She had received dialysis every day because of chronic renal failure for the past 15 years. On a table beside her, there was a mirror and 10-mL syringe on a napkin. A stopper was out of place in a portion of a three-way blood access tube established in ...
journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:10.1111/j.1556-4029.2010.01471.x
更新日期:2010-11-01 00:00:00
abstract::A death of an amateur SCUBA diver with undiagnosed late phase endomyocardial fibrosis is reported. This 27-year-old man found himself in difficulties during a Sub-Aqua Club outing and drowned. Autopsy revealed features of drowning in addition to severe endomyocardial fibrosis (EMF) with collagenization of the left ven...
journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:
更新日期:1993-09-01 00:00:00
abstract::This paper's objective is to describe two cases of fatal commotio cordis resulting from the deliberate striking of children's chests by adults with their fists. These deaths involve two male children, ages 3 years and 14 months. The clinical histories, events in the households prior to the deaths, behaviors of the chi...
journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:
更新日期:2000-05-01 00:00:00
abstract::Cardiovascular disease is the leading cause of sudden death in the world. The etiology of sudden cardiac death involves a wide range of diseases, but seldom pericardial cysts. A pericardial cyst is an uncommon cyst usually located in the middle mediastinum and rarely in the posterior part. They are usually harmless an...
journal_title:Journal of forensic sciences
pub_type:
doi:10.1111/1556-4029.13826
更新日期:2019-01-01 00:00:00
abstract::A case of multiple animal bite wounds is presented along with the techniques used to apprehend the culprits. The victim was a 13 month-old black female who had in excess of 80 puncture wounds. Initially the wounds were thought to be caused by a mechanical instrument but, after an autopsy, were determined to be caused ...
journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:
更新日期:1975-04-01 00:00:00
abstract::This study conducts an investigation of fatal falls from height, examines gender differences, and compares our findings with those of Western countries. We review deaths in Taiwan caused by falls from height that underwent forensic autopsy from 1994 to 2010. Among the examined cases, 182 were suicide, 156 were acciden...
journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:10.1111/1556-4029.12445
更新日期:2014-07-01 00:00:00
abstract::Determination of sex of unknown skeleton remains is the most important step in the identification process. Racial and regional differences in the populations create and maintain specificity in their dimorphic characteristics. Moreover, considering continued secular changes in the population structure, constant revisio...
journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:10.1111/j.1556-4029.2011.01885.x
更新日期:2012-01-01 00:00:00
abstract::Additional studies were performed with respect to examining the lower limits at which canines can reliably detect products commonly used as accelerants and distinguish them from pyrolysis products or background hydrocarbons. As part of a testing exercise performed in conjunction with a national conference of the Canin...
journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:
更新日期:1996-09-01 00:00:00
abstract::Discriminant functions designed for the determination of sex from metacarpal measurements are presented. Three samples of metacarpal specimens were employed in the analysis; one consisting of 212 individuals from the Terry Collection, one of 33 individuals from the Royal Free Medical School in London, and finally, 40 ...
journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:
更新日期:1995-09-01 00:00:00
abstract::Concerns over the toxic by-products produced by traditional ammunition have led to an increase in popularity of nontoxic ammunition. In this work, the chemical composition of six brands of nontoxic ammunition was investigated and compared to that of a road flare, which served as an environmental source with similar co...
journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:10.1111/1556-4029.12881
更新日期:2016-01-01 00:00:00
abstract::Sex determination of the human skeleton is best assessed from the os coxa. The present study explored the possibility of using three-dimensional landmark coordinate data collected from various landmarks located over the entire bone to determine whether there were significant sex differences local to the landmarks. Thi...
journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:10.1111/j.1556-4029.2010.01374.x
更新日期:2010-07-01 00:00:00
abstract::The United States Supreme Court, in the recent case of Riggins v. Nevada, extended its examination of the issue of involuntary treatment with anti-psychotic medication to the mentally disabled facing criminal trial. Although this was an extreme case where the defendant faced a possible death sentence, the involuntary ...
journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:
更新日期:1993-11-01 00:00:00
abstract::We report a case of a 58-year-old man who committed suicide using a modern crossbow. The victim shot himself in the chest with a conical field-tip arrow from close proximity. We first presumed that this was a case of homicide committed with a firearm. We were, however, subsequently proved wrong. The reasons for the pr...
journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:10.1111/j.1556-4029.2010.01666.x
更新日期:2011-03-01 00:00:00
abstract::The duty to protect, or Tarasoff duty, has been conceptualized as arising solely in the context of a clinical setting. A recent California Supreme Court ruling in People v. Clark adds legal, clinical, and ethical dilemmas to the oftentimes contentious Tarasoff issue. Though the Tarasoff issue is but a minor legal poin...
journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:
更新日期:1991-05-01 00:00:00
abstract::Moderate to high levels of alcohol decrease brain intracellular free magnesium concentration, a factor known to be critical in brain injury. Phosphorus magnetic resonance spectroscopy was used to examine changes to brain free magnesium concentration after blunt cranial trauma in alcohol-intoxicated rats. Rats exposed ...
journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:10.1111/j.1556-4029.2012.02108.x
更新日期:2012-07-01 00:00:00
abstract::Between February 2017 and August 2018, West Virginia University conducted a reliability study to determine expert performance among forensic footwear examiners in the United States. Throughout the study's duration, 70 examiners each performed 12 comparisons and reported a total of 840 conclusions. In order to assess t...
journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:10.1111/1556-4029.14551
更新日期:2020-11-01 00:00:00
abstract::Work leading to an improved technique for firearm discharge residue detection by neutron activation is described. The troublesome and time-consuming postirradiation chemistry has been eliminated; also, sample size has been minimized to accommodate some 130 samples per irradiation capsule. To put the method in a proper...
journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:
更新日期:1975-01-01 00:00:00
abstract::Schizophrenia is a chronic disorder that is associated with increased mortality. Although traumatic deaths account for most of this increase, there is also an increased rate of natural deaths in this population. Altered autonomic physiology in this group might contribute to death. To determine if there are schizophren...
journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::A case is presented of a 53-year-old white male who successfully committed suicide by stabbing himself in the abdomen and chest, after which he was driven 11 km (seven miles) to a church without anybody noticing his wounds. In the church he finished his task by stabbing himself through the calvarium. ...
journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:
更新日期:1980-07-01 00:00:00
abstract::In order to establish an animal model for entomotoxicological studies, the kinetics of morphine elimination from blood after a single intravenous injection of morphine and the concentration of morphine in tissues following a continuous perfusion were studied. The aim of these experiments was to obtain controlled morph...
journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:
更新日期:1999-03-01 00:00:00
abstract::Drug-impaired driving is a complex area of forensic toxicology due in part to limited data concerning the type of drugs involved and the concentrations detected. This study analyzed toxicological findings in drivers from fatal motor vehicle collisions (FMVCs) in Ontario, Canada, over a one-year period using a standard...
journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:10.1111/1556-4029.12725
更新日期:2015-05-01 00:00:00
abstract::Ammunition having mercury fulminate-based primers are commonly manufactured by Eastern Bloc countries and used extensively in the Middle East. Gunshot residue (GSR) particles formed by firing these types of ammunition were examined. It was observed that much lower percentage of mercury-containing GSR particles were fo...
journal_title:Journal of forensic sciences
pub_type: 杂志文章
doi:
更新日期:1992-11-01 00:00:00