Abstract：
:A new set of multiplexed PCR primers has been applied to the analysis of human skeletal remains to determine their efficacy in analyzing degraded DNA. These primer sets, known as Miniplexes, produce shorter amplicons (50-280 base pairs (bp)) than standard short tandem repeat (STR) kits, but still utilize the 13 CODIS STR loci, providing results that are searchable on national DNA databases. In this study, a set of 31 different human remains were exposed to a variety of environmental conditions, extracted, and amplified with commercial and Miniplex DNA typing kits. The amplification efficiency of the Miniplex sets was then compared with the Promega PowerPlex 16 system. Sixty-four percent of the samples generated full profiles when amplified with the Miniplexes, while only 16% of the samples generated full profiles with the Powerplex 16 kit. Complete profiles were obtained for 11 of the 12 Miniplex loci with amplicon sizes less than 200 bp. These data suggest smaller PCR amplicons may provide a useful alternative to mitochondrial DNA for anthropological and forensic analysis of degraded DNA from human skeletal remains.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
Opel KL,Chung DT,Drábek J,Tatarek NE,Jantz LM,McCord BRdoi
10.1111/j.1556-4029.2006.00077.xkeywords:
subject
Has Abstractpub_date
2006-03-01 00:00:00pages
351-6issue
2eissn
0022-1198issn
1556-4029pii
JFO77journal_volume
51pub_type
杂志文章abstract：:This report describes the genetic identification of James "Earthquake McGoon" McGovern, a WWII fighter ace who perished in Laos while providing supplies to French troops during the French Indochina war. Because reference samples were unavailable for all of the potential casualties, testing of the entire mitochondrial ...
journal_title：Journal of forensic sciences
pub_type： 历史文章,杂志文章
doi：10.1111/j.1556-4029.2007.00506.x
更新日期：2007-09-01 00:00:00
abstract：:An ELISA method for the detection of salivary amylase in dried stains using a monoclonal anti-human salivary amylase antibody was developed. Studies demonstrated the assay to be sensitive down to 0.0002 Sigma units and showed a linear response between absorbance and salivary amylase activity between 0.002 and 0.2 unit...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2005-07-01 00:00:00
abstract：:The short tandem repeat systems (STRs) HumvWA, HumFXIIIB, and HumFES/FPS were amplified in a triplex polymerase chain reaction (PCR) on blood samples from 100 unrelated Yemenians and 100 unrelated Egyptians. The samples were analyzed by native horizontal discontinual electrophoresis. No deviations from Hardy-Weinberg ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1998-07-01 00:00:00
abstract：:Ignitable liquid residues recovered from a fire scene will often show signs of weathering as a result of exposure to the heat of the fire. In addition, when the substrate is rich in organic matter, both weathering and microbial degradation may be observed. In this study, 20 μL aliquots of fresh gasoline samples were i...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2011.01989.x
更新日期：2012-01-01 00:00:00
abstract：:In this work, we present a measuring methodology for long bones of the limbs (humerus, femur, and tibia) of human corpses. Measurements of cadaveric height and long bone lengths were conducted on 72 corpses (20 females and 52 males) from the School of Medicine at the National Autonomous University of Mexico. Additiona...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12459
更新日期：2014-09-01 00:00:00
abstract：:The effect of temperature on microbial fermentation in blood was studied. Specimens of human blood from a blood bank were inoculated with Candida albicans, an organism capable of causing fermentation. A preservative was added to a portion of the inoculated specimens. These inoculated specimens, as well as uninoculated...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:The current clandestine methodology for the manufacture of illicit cocaine hydrochloride utilizes microwave heating in order to dry the finished product. This study addresses the effects this step has on the occluded solvents present in newly prepared cocaine hydrochloride. Nine 1-kilogram-sized batches of cocaine hyd...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2000-09-01 00:00:00
abstract：:Comparing skeletal structures between antemortem and postmortem chest radiographs is widely used by forensic specialists from many disciplines to positively identify unknown decedents. However, validity assessments of this method have been fairly limited. This study had three objectives: 1) to quantify the reliability...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2002-07-01 00:00:00
abstract：:The fingernails of Mary Sullivan, the last victim of the Boston Strangler, were examined to determine if any genetic information about the murderer could be obtained. The nails were extremely friable necessitating the development of new techniques for isolating and purifying DNA. DNA yields from nails were optimized b...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2003-03-01 00:00:00
abstract：:A case of multiple animal bite wounds is presented along with the techniques used to apprehend the culprits. The victim was a 13 month-old black female who had in excess of 80 puncture wounds. Initially the wounds were thought to be caused by a mechanical instrument but, after an autopsy, were determined to be caused ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1975-04-01 00:00:00
abstract：:The scientific community and the judicial system are different components of society with different structures and functions. Nevertheless, science can contribute relevant and useful information to judicial deliberations if the inherent limitations of that information are understood. These limitations stem from the wa...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2001-07-01 00:00:00
abstract：:This project evaluated the utility of the frontonasal suture for estimating age at death. Utilizing human remains of known age at death with varying degrees of fusion, curated at the American Museum of Natural History in New York City and the Smithsonian Institution's National Museum of Natural History in Washington, ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2012.02193.x
更新日期：2013-01-01 00:00:00
abstract：:Human ecchinococcosis also known as hydatid disease is a zoonotic infection caused by the tapeworm Ecchinococcus with 2-3 Million cases worldwide. We hereby report a 6 years period study of Sudden death due to hydatidosis aiming to analyze the epidemiological criteria, death circumstances, and autopsy observations att...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12172
更新日期：2013-09-01 00:00:00
abstract：:The paper is concerned with the strength of DNA evidence when a suspect is identified via a search through a database of the DNA profiles of known individuals. Consideration of the appropriate likelihood ratio shows that in this setting the DNA evidence is (slightly) stronger than when a suspect is identified by other...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
abstract：:Pharmacokinetic studies of psilocybin in humans have shown the rapid dephosphorylation of psilocybin to psilocin with further conversion to 4-hydroxy-tryptophole (4HT) and 4-hydroxyindole-3-acetic acid (4HIAA) in plasma. Our study shows that psilocin also undergoes conjugation and can be found in the urine as the psil...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2001-05-01 00:00:00
abstract：:An improved gas chromatographic/mass spectrometric (GC/MS) assay is described for the quantitation of codeine and morphine as trimethylsyl (TMS) derivatives. The TMS derivatization of ketone-containing opiates results in the formation of multiple derivatives. Some of these products have retention times close to those ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1998-11-01 00:00:00
abstract：:We evaluated the homogeneity of drug concentrations in muscle in 14 cadavers, comprising 11 drug overdoses and three cases of chronic therapeutic drug use. Analyses were performed on samples from twelve named muscles and femoral venous blood. Standard analytical techniques and instrumentation were used throughout. The...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:The in vitro accuracy and precision of four infrared breath-alcohol analyzers, the Alcotest 7010, BAC Verifier, Intoxilyzer 5000, and Intoximeter 3000, were studied with a protocol adapted from portions of the U.S. Department of Transportation's (DOT) "Standard for Devices to Measure Breath Alcohol." Statistical evalu...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：:Illegal trade in snake parts has increased enormously. In spite of strict protection under wildlife act, a large number of snakes are being killed ruthlessly in India for venom and skin. Here, an interesting case involving confiscation of crystallized dried snake venom and subsequent DNA-based species identification i...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2011.02049.x
更新日期：2012-05-01 00:00:00
abstract：POPULATION:Blood samples were collected from a total of 84 healthy and unrelated Halakki (44) and Kunabhi (40) populations, with their informed written consent. The geographic location of the sampled area is shown in Fig. 1. Both the populations are endogamous, and they belong to Dravidian linguistic family. Halakki is...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2005.00038.x
更新日期：2006-01-01 00:00:00
abstract：:In the era of Daubert and other judicial rulings pertaining to the acceptability of forensic evidence, it is increasingly important that experts are able to testify that their methods have been scientifically tested and that error rates and other factors relating to reliability have been published. The purpose of this...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2003-11-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2010.01431.x
更新日期：2010-09-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章,评审
doi：
更新日期：1995-03-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 历史文章,杂志文章
doi：10.1111/j.1556-4029.2009.01210.x
更新日期：2010-01-01 00:00:00
abstract：:We present three cases of fatal dog maulings of infants placed in mobile infant swings, a phenomenon not previously described in the literature. In each case, the victim was left in a mobile swing, unsupervised by an adult, and the attacking dog was a family pet. Case 1 involved an 18-day-old male infant attacked by a...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2006.00070.x
更新日期：2006-03-01 00:00:00
abstract：:Since forensic facial approximations are used to promote recognition of a deceased person, an accurate forensic facial approximation (FFA) should be easily recognized as the person to whom the skull belonged (target individual). However, the accuracy of FFAs has been previously assessed by the direct comparison of an ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2002-03-01 00:00:00
abstract：:The primary objective of this paper is to report on the successful implementation of forensic facial approximation in a real case in the forensic context. A three-dimensional (3D) facial approximation protocol of the skull was performed with free software, applying techniques in a virtual environment that have already...
journal_title：Journal of forensic sciences
pub_type：
doi：10.1111/1556-4029.14587
更新日期：2021-01-01 00:00:00
abstract：:Experiments were performed to determine the extent of cross-contamination of DNA resulting from secondary transfer due to fingerprint brushes used on multiple items of evidence. Analysis of both standard and low copy number (LCN) STR was performed. Two different procedures were used to enhance sensitivity, post-PCR cl...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12911
更新日期：2016-01-01 00:00:00
abstract：:Advances in the technology employed for the manufacture of glass have resulted in a final glass product with little variability in terms of its physical and optical properties. For example, the refractive index of Australian float glass tends to lie between 1.5189 and 1.5194. It has therefore become necessary to compl...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2007.00644.x
更新日期：2008-03-01 00:00:00
abstract：:This study evaluated the reliability of Widmark calculations, based on breath ethanol reading (BrACs), for estimating the amount of alcohol consumed. A standard ethanol dose (males 0.51 g/kg; females 0.43 g/kg) was given to 115 college seniors, and BrACs were measured for two hours. Calculations of ethanol dose were p...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
