Abstract:
:The fingernails of Mary Sullivan, the last victim of the Boston Strangler, were examined to determine if any genetic information about the murderer could be obtained. The nails were extremely friable necessitating the development of new techniques for isolating and purifying DNA. DNA yields from nails were optimized by using a NaOH-based preparation technique, which was simple, efficient, and minimized handling. Methods for selectively and thoroughly removing exogenous material on nails were also developed through use of a species-specific PCR assay, wherein mitochondrial DNA from the nail could easily be differentiated from DNA of contaminating cells.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
Cline RE,Laurent NM,Foran DRkeywords:
subject
Has Abstractpub_date
2003-03-01 00:00:00pages
328-33issue
2eissn
0022-1198issn
1556-4029journal_volume
48pub_type
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