The fingernails of Mary Sullivan: developing reliable methods for selectively isolating endogenous and exogenous DNA from evidence.

Abstract:

:The fingernails of Mary Sullivan, the last victim of the Boston Strangler, were examined to determine if any genetic information about the murderer could be obtained. The nails were extremely friable necessitating the development of new techniques for isolating and purifying DNA. DNA yields from nails were optimized by using a NaOH-based preparation technique, which was simple, efficient, and minimized handling. Methods for selectively and thoroughly removing exogenous material on nails were also developed through use of a species-specific PCR assay, wherein mitochondrial DNA from the nail could easily be differentiated from DNA of contaminating cells.

journal_name

J Forensic Sci

authors

Cline RE,Laurent NM,Foran DR

keywords:

subject

Has Abstract

pub_date

2003-03-01 00:00:00

pages

328-33

issue

2

eissn

0022-1198

issn

1556-4029

journal_volume

48

pub_type

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