Abstract：
:It has been a challenge to extract DNA from bones previously soaked in water, burned, or buried for a long time, due to the reduced quality and quantity of DNA in the bone samples. The dramatic degradation of the DNA and the presence of PCR inhibitors in the collagen significantly complicate the process of DNA identification in dated and charred bones. In this article, we present a novel strategy to obtain DNA from bones based on the use of cetyltrimethylammonium bromide (CTAB) lysis buffer and isoamyl alcohol-chloroform extraction with subsequent DNA purification using the DNA IQ System, or alternatively the QIAquick system. When applied to bones soaked, burned or buried for up to nine years, this method increases the purity and yield of DNA with respect to the traditional phenol-chloroform method and significantly improves multiplex STR genotyping using fluorescence-based methods. The results of this research will assist forensic scientists in the identification of DNA from victims whose bodies underwent significant trauma or burning, precluding the utilization of traditional forensic DNA identification techniques.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
Ye J,Ji A,Parra EJ,Zheng X,Jiang C,Zhao X,Hu L,Tu Zkeywords:
subject
Has Abstractpub_date
2004-07-01 00:00:00pages
754-9issue
4eissn
0022-1198issn
1556-4029journal_volume
49pub_type
杂志文章abstract：:This study extends previous work on the sorption of explosives to the hair matrix. Specifically, we have studied the interaction of 2,4,6-trinitrotoluene (TNT) and triacetone triperoxide (TATP) as a function of chemical pretreatment with acetonitrile, neutral and alkaline hydrogen peroxide, methanolic KOH and potassiu...
journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
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journal_title：Journal of forensic sciences
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doi：
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